Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,399,517 G→A 8.1% L125F (CTC→TTC)  PP_3878 ← minor capsid protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,399,5170GA 8.1% 212.1 / 6.3 74L125F (CTC→TTC) PP_3878minor capsid protein C
Reads supporting (aligned to +/- strand):  ref base G (29/39);  new base A (3/3);  total (32/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

CAGTGCAAGCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTC  >  NC_002947/4399370‑4399662
                                                                                                                                                   |                                                                                                                                                 
cAGTGCAAGCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAg                                                                                                                                                   <  2:161246/148‑1 (MQ=255)
 aGTGCAAGCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGca                                                                                                                                                 <  3:14227/149‑1 (MQ=255)
   tGCAAGCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAAcaca                                                                                                                                               <  4:22807/149‑1 (MQ=255)
    gCAAGCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACaa                                                                                                                                              >  8:87552/1‑149 (MQ=255)
     cAAGCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAAt                                                                                                                                             <  1:110871/149‑1 (MQ=255)
     cAAGCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAAt                                                                                                                                             <  1:25851/149‑1 (MQ=255)
      aaGCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAACACATtg                                                                                                                                            >  5:337278/1‑149 (MQ=255)
      aaGCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAACACATtg                                                                                                                                            >  1:138849/1‑149 (MQ=255)
       aGCAGAGGCATTCCGGTACCCCCCTGAGTACGCCATGATGTTAACCAGGCCGGTGATTGGTTTGATCTTGGGCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAAtgt                                                                                                                                           <  4:304993/149‑1 (MQ=255)
        gCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAAtgtg                                                                                                                                          >  6:229220/1‑149 (MQ=255)
         cAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAACACATtgtgc                                                                                                                                         <  6:337278/149‑2 (MQ=255)
             cGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGa                                                                                                                                     <  3:338510/149‑1 (MQ=255)
             cGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAACACATTGTGCTCGa                                                                                                                                     <  2:138849/149‑1 (MQ=255)
              gCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTc                                                                                                                                       >  4:87622/1‑146 (MQ=255)
              gCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTc                                                                                                                                       <  3:87622/146‑1 (MQ=255)
                 aTCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCAt                                                                                                                                 <  4:218033/149‑1 (MQ=255)
                 aTCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCAt                                                                                                                                 >  8:137428/1‑149 (MQ=255)
                  tCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATg                                                                                                                                <  2:101347/149‑1 (MQ=255)
                    aGGTAACCCCCTGAGTACGACATGAAGTTAACCAGGCCGTTGATTGGTTTTATCTTGGTCGCTGCCCGAATGTGCGCCGCCAGTGCAAAAGCGCCGAGGGCGCTGCCGCCTGGTCTGTCAATGTCGAGCACAATGTGTTCGACGATggg                                                                                                                              <  6:295591/149‑1 (MQ=255)
                       tAATCCGCTGAGTACGGCATGAAGTTAACGCGGCCGGTGAGTTGGTTGTTCTTGGTCGCGGCACGAATGTCCGCCGCCAGTTCAAAATCGCCTACGGCGCTGCCTCCTGGGCTGTCAATGTCGAGCACAATGTATTCGACCATGGGGTc                                                                                                                           <  2:319983/149‑1 (MQ=255)
                       tAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTc                                                                                                                           <  2:246126/149‑1 (MQ=255)
                          cccccTGAGTACGCAATGAAGTTAACCTGCCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGCCGCCGCTGCCGCCCGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGt                                                                                                                        <  1:110700/149‑1 (MQ=255)
                           ccccTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGt                                                                                                                        >  6:131402/1‑148 (MQ=255)
                             ccTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGAt                                                                                                                     >  7:51693/1‑149 (MQ=255)
                                 aGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGc                                                                                                                  <  8:298920/148‑1 (MQ=255)
                                      gCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTAtt                                                                                                            >  3:61873/1‑149 (MQ=255)
                                      gCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTAt                                                                                                             >  3:103174/1‑148 (MQ=255)
                                        cATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTAtt                                                                                                            >  4:138565/1‑147 (MQ=255)
                                         aTGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAg                                                                                                         >  6:3722/1‑149 (MQ=255)
                                          tGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAgg                                                                                                        <  5:192728/149‑1 (MQ=255)
                                                 aaCCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGtt                                                                                                                                        <  6:211525/110‑1 (MQ=255)
                                                 aaCCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGtt                                                                                                                                        >  5:211525/1‑110 (MQ=255)
                                                 aaCCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTc                                                                                                 >  3:193047/1‑149 (MQ=255)
                                                    cAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGgctgcgag                                                                                                >  3:277201/1‑144 (MQ=255)
                                                       gCCGGTTATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATg                                                                                                                    <  7:212185/124‑1 (MQ=255)
                                                       gCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATg                                                                                                                    >  8:212185/1‑124 (MQ=255)
                                                        ccGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGccc                                                                                          >  3:91666/1‑149 (MQ=255)
                                                        ccGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGccc                                                                                          <  5:207055/149‑1 (MQ=255)
                                                          ggTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCtt                                                                                        <  7:87552/149‑1 (MQ=255)
                                                           gTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTc                                                                                       <  4:193047/149‑1 (MQ=255)
                                                            tGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCa                                                                                      >  8:321266/1‑149 (MQ=255)
                                                              aTTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCa                                                                                      >  2:172012/1‑147 (MQ=255)
                                                                tGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAATGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGc                                                                                  >  1:267874/1‑149 (MQ=255)
                                                                 ggTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATg                                                                                                                    >  1:124723/1‑114 (MQ=255)
                                                                 ggTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATg                                                                                                                    <  2:124723/114‑1 (MQ=255)
                                                                 ggTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCGAGCAGCCCtgaataag                                                                                  <  4:277201/148‑8 (MQ=255)
                                                                  gTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTg                                                                                >  4:354513/1‑149 (MQ=255)
                                                                      gATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTc                                                                             <  5:229220/148‑1 (MQ=255)
                                                                            ggTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATg                                                                          <  3:138565/145‑1 (MQ=255)
                                                                             gTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGtctc                                                                     >  4:217536/1‑149 (MQ=255)
                                                                                gcgGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCg                                                                    >  2:296112/1‑147 (MQ=255)
                                                                                 cgGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAg                                                                 >  2:252657/1‑149 (MQ=255)
                                                                                   gCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGc                                                               <  4:103174/149‑1 (MQ=255)
                                                                                         aTGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCaa                                                         <  5:131402/149‑1 (MQ=255)
                                                                                                    cAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCcgc                                              <  7:137428/149‑1 (MQ=255)
                                                                                                    cAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCcgc                                              <  4:91666/149‑1 (MQ=255)
                                                                                                      gTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGa                                            <  3:354513/149‑1 (MQ=255)
                                                                                                          aaaaGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTca                                        <  8:51693/149‑1 (MQ=255)
                                                                                                           gcaGCGCCGTCGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTc                                                                                                 >  7:25700/3‑91 (MQ=255)
                                                                                                           gcaGCGCCGTCGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTc                                                                                                 <  8:25700/89‑1 (MQ=255)
                                                                                                                gcCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGaa                                  <  2:267874/149‑1 (MQ=255)
                                                                                                                 ccGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAAc                                 <  7:321266/149‑1 (MQ=255)
                                                                                                                 ccGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAAc                                 <  5:3722/149‑1 (MQ=255)
                                                                                                                      ggCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCAt                            <  3:217536/149‑1 (MQ=255)
                                                                                                                         gcTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGa                         <  7:280073/149‑1 (MQ=255)
                                                                                                                                 cTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATc                 <  1:296112/149‑1 (MQ=255)
                                                                                                                                   gggCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTgg                                                                               >  4:135276/1‑85 (MQ=255)
                                                                                                                                   gggCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTgg                                                                               <  3:135276/85‑1 (MQ=255)
                                                                                                                                        gTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCca          >  1:324406/1‑149 (MQ=255)
                                                                                                                                         ccatggTCGAACACATTGTGCTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCcac         >  8:250671/6‑149 (MQ=255)
                                                                                                                                          cAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTAATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCcaca        >  7:128175/1‑149 (MQ=255)
                                                                                                                                            aTGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACAcc      <  4:61873/149‑1 (MQ=255)
                                                                                                                                                cGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCCGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTc  >  8:231813/1‑149 (MQ=255)
                                                                                                                                                 gAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCcacgc       <  2:30923/143‑3 (MQ=255)
                                                                                                                                                 gAGCACAATGTGTTCGACCATGGGGTCAGTCATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCcacgc       >  1:30923/1‑141 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CAGTGCAAGCAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTC  >  NC_002947/4399370‑4399662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑