Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,418,167 G→C 10.6% E75Q (GAG→CAG)  PP_3907 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,418,1670GC10.6% 135.8 / 6.4 47E75Q (GAG→CAG) PP_3907hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/20);  new base C (2/3);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

CAGTGGTGAACATTGACCCCAGCGGTCAGCAGGTGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATACCGACCTGCGGGCCACGGCCAACCCCAATCACCTGGTGGCGGCCGGCTGGATCG  >  NC_002947/4418024‑4418314
                                                                                                                                               |                                                                                                                                                   
cAGTGGTGAACATTGACCCCAGCGGTCAGCAGGTGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTc                                                                                                                                                <  7:30192/149‑1 (MQ=255)
   tggtgAACATTGACCCCAGCGGTCAGCAGGTGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCTTACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGc                                                                                                                                             <  1:308791/149‑1 (MQ=255)
     gtgAACATTGACCCCAGCGGTCAGCAGGTGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGc                                                                                                                                             >  2:273880/1‑147 (MQ=255)
      tgAACATTGACCCCAGCGGTCAGCAGGTGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAg                                                                                                                                          <  7:250914/149‑1 (MQ=255)
      tgAACATTGACCCCAGCGGTCAGCAGGTGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGAACTCAGTCCGGcaaa                                                                                                                                          <  3:101438/149‑4 (MQ=255)
        aaCATTGACCCCAGCGGTCAGCAGGTGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGa                                                                                                                                                    >  7:269715/1‑137 (MQ=255)
        aaCATTGACCCCAGCGGTCAGCAGGTGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGa                                                                                                                                                    <  8:269715/137‑1 (MQ=255)
                  ccAGCGGTCAGCAGGTGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAg                                                                                                                              >  7:86358/1‑149 (MQ=255)
                    aGCGGTCAGCAGGTGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGtc                                                                                                                            >  1:340459/1‑149 (MQ=255)
                           agcagGTGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGAACTCAGTCCgg                                                                                                                                              <  1:371415/124‑1 (MQ=255)
                           agcagGTGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGAACTCAGTCCgg                                                                                                                                              >  2:371415/1‑124 (MQ=255)
                               ggTGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTATGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTg                                                                                                                 <  1:273880/149‑1 (MQ=255)
                                 tGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTgcg                                                                                                               >  1:42394/1‑149 (MQ=255)
                                  gCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTacaa                                                                                                                                  <  2:245371/129‑1 (MQ=255)
                                      aTCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGTTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACAATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCgg                                                                                                                                       >  7:270670/1‑120 (MQ=255)
                                          aCAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCATCGTGCTTTGCCGGACTGAGTTCGGCGAGCTGTACAACAAGTCGATCGAGGTTgc                                                                                                                <  4:108480/139‑1 (MQ=255)
                                          aCAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTgc                                                                                                                >  3:108480/1‑139 (MQ=255)
                                            aGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGc                                                                                                                                         <  3:159878/112‑1 (MQ=255)
                                            aGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGc                                                                                                                                         >  4:159878/1‑112 (MQ=255)
                                             ggAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAAc                                                                                                   <  8:159488/149‑1 (MQ=255)
                                              gaagaaCCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGCGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAAc                                                                                                   >  6:316323/1‑148 (MQ=255)
                                                 gaaCCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGAACTCAGTCCGGca                                                                                                                                            <  2:276185/104‑2 (MQ=255)
                                                 gaaCCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGAACTCAGTCCGGca                                                                                                                                            >  1:276185/1‑103 (MQ=255)
                                                            aGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCAc                                                                                    <  3:118345/149‑1 (MQ=255)
                                                                     tACGCCAGGTCGTCTACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGGTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTTCGCCGCAAGGCAACTACCGCGCCGAGCACCtgactgac                                                                           <  7:313289/149‑1 (MQ=255)
                                                                     tACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCtgactgac                                                                           <  3:121481/149‑1 (MQ=255)
                                                                        gCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAg                                                                                                                     <  2:118542/104‑1 (MQ=255)
                                                                        gCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAg                                                                                                                     >  1:118542/1‑104 (MQ=255)
                                                                          cAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGAt                                                                      >  1:159560/1‑149 (MQ=255)
                                                                                gtcgACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTa                                                                                                                                     >  3:31676/1‑80 (MQ=255)
                                                                                gtcgACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTa                                                                                                                                     <  4:31676/80‑1 (MQ=255)
                                                                                gtcgACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAgg                                                                 <  2:159560/148‑1 (MQ=255)
                                                                                       cgGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCtat                                                         <  5:360833/149‑1 (MQ=255)
                                                                                               gATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATAcc                                                      <  4:290659/144‑1 (MQ=255)
                                                                                               gATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATAcc                                                      >  3:290659/1‑144 (MQ=255)
                                                                                                          ccGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATACCGACCTGCGGGCCACgg                                      <  2:42394/149‑1 (MQ=255)
                                                                                                                gACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATACCGACCTGCGGGCCACGGCCAAcc                                >  3:30818/1‑149 (MQ=255)
                                                                                                                  ccATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATACCGACCTGCGGGCCACGGCCAAccc                               >  3:63166/1‑148 (MQ=255)
                                                                                                                   cATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATACCGACCTGCGGGCCACGGCCAAcccc                              >  2:350701/1‑148 (MQ=255)
                                                                                                                         tctcAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATACCGACCTGCGGGCCACGGCCAACCCCAATCAcc                       >  4:355304/1‑149 (MQ=255)
                                                                                                                             aGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATACCGACCTGCGGGCCACGGCCAACCCCAATCACCtggt                   <  3:355304/149‑1 (MQ=255)
                                                                                                                             aGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATACAGACCTGCGGGCCACGGCCAACCCCAATCACCtggt                   >  8:142965/1‑149 (MQ=255)
                                                                                                                                  gCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATACCGACCTGCGGGCCACGGCCAACCCCAATCACCTGGTggcgg              <  1:371855/149‑1 (MQ=255)
                                                                                                                                    tttGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATACCGACCTGCGGGCCACGGCCAACACCAATCACCTGGTGGcggcc            >  3:141956/1‑149 (MQ=255)
                                                                                                                                    tttGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGACGCGACCTATACCGACCTGCGGGCCACGGCCAACCCCAATCACCTGGTGGcggcc            >  4:307920/1‑149 (MQ=255)
                                                                                                                                       gCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATACCGACCTGCGGGCCACGGCCAACCCCAATCACCTGGTGGcggccggc         >  3:309510/1‑149 (MQ=255)
                                                                                                                                              tGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATACCGACCTGCGGGCCACGGCCAACCCCAATCACCTGGTGGCGGCCGGCTGGATCg  >  3:62155/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
CAGTGGTGAACATTGACCCCAGCGGTCAGCAGGTGCTGATCAACAGGAAGAACCTCAAGCAGATCCGCATACGCCAGGTCGTCGACGCGGTTTGCGATATCCCGCACCGGTGGACCATCTATCTCAGCGTGCTTTGCCGGACTGAGTTCGGCGAGCGGTACAACAAGTCGATCGAGGTTGCGCCGCAAGGCAACTACCGCGCCGAGCACCTGACTGACGTGATCGAGGCGACCTATACCGACCTGCGGGCCACGGCCAACCCCAATCACCTGGTGGCGGCCGGCTGGATCG  >  NC_002947/4418024‑4418314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑