Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 48,873 C→A 8.1% D168Y (GAC→TAC) ‡ czcA‑I ← cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294748,8730CA 8.1% 219.5 / 6.0 70D168Y (GAC→TAC) ‡czcA‑Ication efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (35/29);  new base A (3/3);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

ACGCTGCGACCAGGTCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTCTGGCAGCT  >  NC_002947/48726‑49016
                                                                                                                                                   |                                                                                                                                               
aCGCTGCGACCAGGTCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTcc                                                                                                                                                <  6:379461/149‑1 (MQ=255)
       gACCAGGTCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCa                                                                                                                                         >  8:346192/1‑149 (MQ=255)
         ccAGGTCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCAcg                                                                                                                                       >  1:214581/1‑149 (MQ=255)
         ccAGGTCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGGACTGGATCatc                                                                                                                                       <  6:341277/149‑3 (MQ=255)
         ccAGGTCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGGACTGGATCatc                                                                                                                                       <  2:366790/149‑3 (MQ=255)
           aGGTCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGGACTGGATCatcaa                                                                                                                                     <  7:286469/149‑5 (MQ=255)
                 ttGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAggtc                                                                                                                               >  6:58193/1‑149 (MQ=255)
                        tCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCggg                                                                                                                        <  5:162624/149‑1 (MQ=255)
                        tCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCggg                                                                                                                        <  5:332003/149‑1 (MQ=255)
                        tCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCggg                                                                                                                        >  8:146189/1‑149 (MQ=255)
                        tCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCggg                                                                                                                        <  7:295915/149‑1 (MQ=255)
                          aGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCgggg                                                                                                                       <  5:208275/148‑1 (MQ=255)
                             ttGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTa                                                                                                                   >  3:370970/1‑149 (MQ=255)
                                   gtagCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTg                                                                                                                                              <  1:254731/116‑1 (MQ=255)
                                   gtagCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTg                                                                                                                                              >  2:254731/1‑116 (MQ=255)
                                       ccAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGAGCAGGTCGGTCGGGGTGTACGGCGTACCg                                                                                                         >  7:89781/1‑149 (MQ=255)
                                               tttGGATCCGGCGCAACCAGGAACTGCTTGGCTTAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCct                                                                                                     <  6:206201/145‑1 (MQ=255)
                                               tttGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCct                                                                                                     >  5:206201/1‑145 (MQ=255)
                                                 ttgATCCGGCGCAACCAGGACCTGCTTGGCATCACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCttt                                                                                                 <  8:312129/145‑1 (MQ=255)
                                                 tGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCttt                                                                                                 <  5:58193/147‑1 (MQ=255)
                                                     tCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACcgc                                                                                           >  2:347328/1‑149 (MQ=255)
                                                            cAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGGCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCt                                                                                    <  7:123518/149‑1 (MQ=255)
                                                             aaCCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCgcgc                                                                                         >  7:281800/1‑143 (MQ=255)
                                                             aaCCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCgcgc                                                                                         <  8:281800/143‑1 (MQ=255)
                                                              aCCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTc                                                                                  <  4:370970/149‑1 (MQ=255)
                                                                cAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCggg                                                                                                                        >  7:101927/1‑109 (MQ=255)
                                                                cAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCggg                                                                                                                        <  8:101927/109‑1 (MQ=255)
                                                                   gAACTGCTTAGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCtt                                                                             >  7:340599/1‑149 (MQ=255)
                                                                    aaCTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCtt                                                                             >  5:340924/1‑148 (MQ=255)
                                                                     aCTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCa                                                                           >  3:244286/1‑149 (MQ=255)
                                                                         cTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGc                                                                                                               <  5:9277/109‑1 (MQ=255)
                                                                         cTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGc                                                                                                               >  6:9277/1‑109 (MQ=255)
                                                                         cTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAg                                                                       >  2:271541/1‑149 (MQ=255)
                                                                         cTTGGCATAACCGACTATTGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCGGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACa                                                                        <  7:332776/148‑1 (MQ=255)
                                                                            ggCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTc                                                                     <  7:346192/148‑1 (MQ=255)
                                                                                 aaCCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAg                                                                >  8:135028/1‑148 (MQ=255)
                                                                                   ccgccgATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAgg                                                               >  3:358123/1‑147 (MQ=255)
                                                                                    cgccgATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAgg                                                               >  4:282137/1‑146 (MQ=255)
                                                                                         aTGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGAtctc                                                       <  1:271541/149‑1 (MQ=255)
                                                                                          tGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCg                                                      >  1:309320/1‑149 (MQ=255)
                                                                                           ggTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTc                                                                            >  1:36840/1‑126 (MQ=255)
                                                                                           ggTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTc                                                                            <  2:36840/126‑1 (MQ=255)
                                                                                             aaTTGATCTCGGCCACACCCGGGACGATACGAAGCAGACCCTAGATGAACCAGTCCAGGATCACGCCCAGGTCGGTCGGCGTCTACCGCGTACCATCCTCTTTGACCTCCCCATGGTCCGCTTCACCAGTCCACCGGAAGATCTCGCCg                                                   >  8:250558/2‑149 (MQ=255)
                                                                                               ttGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCg                                                      >  1:371160/1‑144 (MQ=255)
                                                                                               ttGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCg                                                      <  2:371160/144‑1 (MQ=255)
                                                                                                 gATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAgg                                                <  8:340599/148‑1 (MQ=255)
                                                                                                   tctcGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCg                                             >  7:71520/1‑149 (MQ=255)
                                                                                                       ggCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTa                                          <  1:347328/148‑1 (MQ=255)
                                                                                                       ggCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAg                                         >  4:110543/1‑149 (MQ=255)
                                                                                                       ggCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCAAGGCCGGTa                                          <  2:214581/148‑1 (MQ=255)
                                                                                                           acacCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTa                                          >  3:262128/1‑144 (MQ=255)
                                                                                                           acacCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTa                                          >  3:267159/1‑144 (MQ=255)
                                                                                                           acacCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTa                                          <  4:262128/144‑1 (MQ=255)
                                                                                                           acacCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTa                                          <  4:267159/144‑1 (MQ=255)
                                                                                                               ccGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGa                                 >  8:153417/1‑149 (MQ=255)
                                                                                                                 gggACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGAcc                               >  4:336611/1‑149 (MQ=255)
                                                                                                                    aCGTTACGCAGCTGAGGCTTTATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCAt                            <  7:146189/149‑1 (MQ=255)
                                                                                                                       ttACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGAc                         >  2:297451/1‑149 (MQ=255)
                                                                                                                         aCGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACg                        <  6:340924/148‑1 (MQ=255)
                                                                                                                           gCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGcc                     >  1:380942/1‑149 (MQ=255)
                                                                                                                              gCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCctct                  <  7:153417/149‑1 (MQ=255)
                                                                                                                                    tgcgcgtgagCCAGGACTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACAcc             >  5:148172/7‑148 (MQ=255)
                                                                                                                                      ttGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTc          >  6:168966/1‑149 (MQ=255)
                                                                                                                                        gatgatCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTCt         <  2:380942/148‑1 (MQ=255)
                                                                                                                                        gatgatCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTCTg        >  3:83489/1‑149 (MQ=255)
                                                                                                                                        cgtgatCCAGGACTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTCTg        >  7:120605/3‑149 (MQ=255)
                                                                                                                                        cgtgatCCAGGACTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACAGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTCTg        >  6:304528/3‑149 (MQ=255)
                                                                                                                                         atgatCCAGTCCTGTATCACGCGCAGGTAGGTCGGGGTGTACGGCGGACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTCTgg       <  8:89781/149‑1 (MQ=255)
                                                                                                                                           gatCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACCCCTTCTGGct     >  1:356731/1‑148 (MQ=255)
                                                                                                                                              ccAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCACGCCGAGGCCGGTAGATACCGGACCCATGACCGCCTATACACCTACTGGCAGCt  >  6:308385/1‑149 (MQ=255)
                                                                                                                                               cAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTCTGGCAGCt  <  5:61982/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
ACGCTGCGACCAGGTCATTGAGTGTCAGCTTGTAGGTAGCCAGGCGCTTTGGATCCGGCGCAACCAGGAACTGCTTGGCATAACCGCCGATGGTATTGATCTCGGCCACACCCGGGACGTTACGCAGCTGAGGCTTGATGATCCAGTCCTGGATCACGCGCAGGTCGGTCGGGGTGTACGGCGTACCGTCCTCTTTGACCGCGCCATCTTCGGCTTCAACAGTCCACAGGAAGATCTCGCCGAGGCCGGTAGATACCGGACCCATGACGGCCTCTACACCTTCTGGCAGCT  >  NC_002947/48726‑49016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑