Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,505,522	C→G	5.6%	A99G (GCC→GGC)	PP_3997 →	glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,505,522	0	C	G	5.6%	228.8 / 3.8	71	A99G (GCC→GGC)	PP_3997	glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (32/35);  new base G (2/2);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

AGACACCCAGCTCGGTGCCTTCCTCATGCTGCTGCGGCACAAGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCGTATGTATACAGAGCAACTCCTCGACCTGCTGCAGATTC  >  NC_002947/4505382‑4505665                                                                                                                                              |                                                                                                                                                 aGACACCCAGCTCGGTGCCTTCCTCATGCTGCTGCGGCACAAGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCaaga                                                                                                                                          <  1:162634/149‑1 (MQ=255)  gACACCCAGCTCGGTGCCTTCCTCATGCTGCTGCGGCACAAGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCaagaa                                                                                                                                         <  5:209228/149‑1 (MQ=255)    cacCCAGCTCGGTGCCTTCCTCATGCTGCTGCGGCACAAGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGc                                                                                                                                       >  2:142905/1‑149 (MQ=255)            tCGGTGCCTTCCTCATGCTGCTGCGGCACAAGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTg                                                                                                                               >  7:107845/1‑149 (MQ=255)                  tcTTCCTCATGCTGCTGCGGCACAAGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCTGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTg                                                                                                                          <  2:283321/147‑1 (MQ=255)                      ccTCATGCTGCTGCGGCACAAGGAAGAAAGCGCCGATGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTAc                                                                                                                      <  1:234873/148‑1 (MQ=255)                           tgctgctgcGGCACAAGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACctgctg                                                                                                                <  3:241860/149‑1 (MQ=255)                            gctgctgcGGCACAAGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTgg                                                                                                               >  7:91579/1‑149 (MQ=255)                               gctgcGGCACAAGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGCCCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGgccg                                                                                                            >  6:306826/1‑149 (MQ=255)                                      cacaAGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGGCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTg                                                                                                     >  2:226674/1‑149 (MQ=255)                                      cacaAGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGGCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTg                                                                                                     >  2:221205/1‑149 (MQ=255)                                      cacaAGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTg                                                                                                     >  1:136748/1‑149 (MQ=255)                                      cacaAGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTg                                                                                                     >  4:134374/1‑149 (MQ=255)                                         aaGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCt                                                                                                  >  5:61084/1‑149 (MQ=255)                                          aGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTg                                                                                                 <  8:91579/149‑1 (MQ=255)                                          aGGAAGAAAGCGCCGAAGAGCTGGCCGGCGTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTCGACTGGCCCACGTACGCCGGCAAGAAGCGGCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTg                                                                                                 <  2:356075/149‑1 (MQ=255)                                            gaagaaAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGc                                                                                               >  6:157269/1‑149 (MQ=255)                                             aagaaAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGc                                                                                               >  5:179750/1‑148 (MQ=255)                                                aaaGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGAGCCA‑CGCATCGCCGATGACCTGGACTGGCCCACGTACGCCGGCAAGAACCGTCACCTGCCCAGGTACctcc                                                                                                                  >  2:175409/1‑124 (MQ=255)                                                aaaGCGCCGAAGAGCTGGCCGGCTTCAACGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCGGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACctgc                                                                                                                  <  1:175409/126‑1 (MQ=255)                                                    cgcCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATg                                                                                      <  4:57043/149‑1 (MQ=255)                                                     gcCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATgg                                                                                     >  6:357182/1‑149 (MQ=255)                                                      ccGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCAGCGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATgg                                                                                     >  1:289389/1‑149 (MQ=255)                                                      ccGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGc                                                                                    <  3:134374/149‑1 (MQ=255)                                                       cGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCAc                                                                                                                                  <  1:247319/103‑1 (MQ=255)                                                       cGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCAc                                                                                                                                  >  2:247319/1‑103 (MQ=255)                                                        gAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGt                                                                                  <  8:107845/149‑1 (MQ=255)                                                              cTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTg                                                                                                 >  8:188655/1‑129 (MQ=255)                                                              cTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTg                                                                                                 <  7:188655/129‑1 (MQ=255)                                                                 gCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTc                                                                          <  1:50783/148‑1 (MQ=255)                                                                            cGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCAcggcg                                                              >  3:321143/1‑149 (MQ=255)                                                                             gAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGt                                                                                                      >  3:3105/1‑109 (MQ=255)                                                                             gAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGt                                                                                                      <  4:3105/109‑1 (MQ=255)                                                                               ggCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCAcggcggcg                                                           <  2:136748/149‑1 (MQ=255)                                                                                gCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCAcggcggcgg                                                          >  6:141899/1‑149 (MQ=255)                                                                                 ccTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACggcggcggc                                                         >  2:11269/1‑149 (MQ=255)                                                                                      cgcgcCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGGCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGcac                                                     <  1:221205/148‑1 (MQ=255)                                                                                      cgcgcCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGGCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGcac                                                     <  1:226674/148‑1 (MQ=255)                                                                                             aCCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGcc                                             >  2:12265/1‑149 (MQ=255)                                                                                                 gCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGc                                                                    <  3:314302/122‑1 (MQ=255)                                                                                                 gCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGc                                                                    >  4:314302/1‑122 (MQ=255)                                                                                                 gCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGc                                         >  4:250376/1‑149 (MQ=255)                                                                                                  cAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTTGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCg                                        >  1:292446/1‑149 (MQ=255)                                                                                                  cAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGc                                         <  1:142905/148‑1 (MQ=255)                                                                                                  cAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCg                                        >  8:98168/1‑149 (MQ=255)                                                                                                    ggCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCgta                                      <  2:289389/149‑1 (MQ=255)                                                                                                     gcgcCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCT‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCgtat                                     <  5:141899/149‑1 (MQ=255)                                                                                                     gcgcCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCACTCAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCgta                                      <  1:289529/149‑1 (MQ=255)                                                                                                     gcgcCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCgtat                                     <  5:157269/149‑1 (MQ=255)                                                                                                       gcCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCgtatgt                                   <  3:94103/149‑1 (MQ=255)                                                                                                       gcCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCgtatg                                    >  3:336090/1‑148 (MQ=255)                                                                                                        ccA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGgcggcggcg                                                        >  5:30278/1‑127 (MQ=255)                                                                                                        ccA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGgcggcggcg                                                        <  6:30278/127‑1 (MQ=255)                                                                                                         cA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGc                                              <  5:64275/136‑1 (MQ=255)                                                                                                         cA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGc                                              >  6:64275/1‑136 (MQ=255)                                                                                                             gCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCGTATGTATACa                              <  4:321143/149‑1 (MQ=255)                                                                                                                tCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTTGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCGTATGTATACagag                           <  2:292446/149‑1 (MQ=255)                                                                                                                 cGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCgg                                           <  1:149943/132‑1 (MQ=255)                                                                                                                   ccGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCGTATGTATACAGAGCaa                        >  5:14997/1‑149 (MQ=255)                                                                                                                    cGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGca                                                      >  6:27064/1‑118 (MQ=255)                                                                                                                    cGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGca                                                      <  5:27064/118‑1 (MQ=255)                                                                                                                          ccTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGTCGGCGGCGCACACACTGCCGGGCGTATGTATACAGCGCAActcctc                  >  8:143246/1‑148 (MQ=255)                                                                                                                            tGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCGTATGTATACAGAGCAACTCCTCGAc               >  6:64076/1‑149 (MQ=255)                                                                                                                              gACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCGTATGTATACAGAGCAACTCCTCGAcc              <  6:179750/148‑1 (MQ=255)                                                                                                                              gACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCGTATGTATACAGAGCAACTCCTCGAc               <  5:306826/147‑1 (MQ=255)                                                                                                                                 tGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCGTATGTATACagag                           >  2:17509/1‑132 (MQ=255)                                                                                                                                 tGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCGTATGTATACagag                           <  1:17509/132‑1 (MQ=255)                                                                                                                                   gCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGc                                         <  6:229852/116‑1 (MQ=255)                                                                                                                                   gCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCGTATGTATACAGAGCAACTCCTCGACctgctg         >  7:81160/1‑148 (MQ=255)                                                                                                                                     ccACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCGTATGTATTCAGAGCAACTCCTCGACCTGCTGCAg      <  1:11269/149‑1 (MQ=255)                                                                                                                                         gTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCGTATGTATACAGAGCAACTCCTCGACCTGCTGCAGATTc  <  3:250376/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                 AGACACCCAGCTCGGTGCCTTCCTCATGCTGCTGCGGCACAAGGAAGAAAGCGCCGAAGAGCTGGCCGGCTTCACCGAGGCCTTGCGCGCCCACCTGCAGGCGCCA‑CGCATCGCCGTTGACCTGGACTGGCCCACGTACGCCGGCAAGAAGCGTCACCTGCCCTGGTACCTGCTGGCCGCCAAGTGCCTGGCCA‑ACAATGGCGTGCGCATTCTGATGCACGGCGGCGGCGCACACACTGCCGGGCGTATGTATACAGAGCAACTCCTCGACCTGCTGCAGATTC  >  NC_002947/4505382‑4505665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑