| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,530,025 | T→A | 23.3% | intergenic (+31/‑137) | PP_4018 → / → topB | acetyltransferase/DNA topoisomerase III |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,530,025 | 0 | T | A | 23.3% | 47.8 / 12.1 | 30 | intergenic (+31/‑137) | PP_4018/topB | acetyltransferase/DNA topoisomerase III |
| Reads supporting (aligned to +/- strand): ref base T (15/8); new base A (3/4); total (18/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01 | |||||||||||
AGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGCGCCTGT > NC_002947/4529885‑4530171 | aGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCcgcg < 3:333599/149‑1 (MQ=255) cacgccacgcCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAagag < 8:109198/149‑1 (MQ=255) cacgccacgcCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTAAACCCGCGAaga < 7:108261/148‑1 (MQ=255) cacgccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTAGCGGGTTTACCCGCGAAGAGG‑CCgg > 3:286327/1‑149 (MQ=255) gTTCTATGAGCGGCTGGTCTTTCGCTTAGTCAGCGAGTAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTAAACCCGCGaa < 5:41061/135‑1 (MQ=255) gTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTACCCGGGATAACCCGCGaa > 6:41061/1‑135 (MQ=255) ttCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTAAACCCGCGAaga < 1:88288/136‑1 (MQ=255) ttCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTAAACCCGCGAaga > 2:88288/1‑136 (MQ=255) tGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGata > 2:88823/1‑148 (MQ=255) ggCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGtt > 8:27050/1‑114 (MQ=255) ggCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGtt < 7:27050/114‑1 (MQ=255) accgtggAAATCCACGTAGCTGGCATCCCGCATTTCCACATGGTGCGCGTCTGGCTGGCCCTGATCCATCACCTTTTTTGGCCTCTTCGCGGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCt < 1:308382/143‑1 (MQ=255) aGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTg > 1:157191/1‑148 (MQ=255) cTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGTTTTACCCGCTAAGAGGCCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACatccatcc < 1:88823/148‑1 (MQ=255) cATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGc > 5:196348/1‑149 (MQ=255) cGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGcccac > 3:50766/1‑147 (MQ=255) cgACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATcc > 3:88697/1‑149 (MQ=255) aTCACGGGCCCTGGCCTCTCCGCGGGTTAACCCGGTAATAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGAt < 3:254801/149‑1 (MQ=255) ccTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATTTACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAg > 2:1174/1‑149 (MQ=255) cTGGCCTCTTCGCGGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTccc > 1:311039/1‑149 (MQ=255) tGGCCTCTTCGCGGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTcccc > 2:32399/1‑149 (MQ=255) gCCTCTTCGCGGGGTAACCCGCGAAGAGG‑CCGGCTCAGGCGATATAAATACCCGCTGACAAAAAACCTGTACATCCAACCAGATAAAACATGCCTCTGCGCCCCCCCTTGCGCCTCCAATGGCCCCCCACCCCGAGGAAGCGGCCCCgg > 2:190961/1‑149 (MQ=255) ctctTCGCTGGTTTACCCGCGAATAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGcc < 7:83392/149‑1 (MQ=255) ctTCGCGGGTAAACCCGCGAAGAGG‑CCAGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGcc > 4:370677/1‑120 (MQ=255) ctTCGCGGGTAAACCCGCGAAGAGG‑CCAGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGcc < 3:370677/120‑1 (MQ=255) tCGCGGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGc < 7:215471/135‑1 (MQ=255) tCGCGGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGc > 8:215471/1‑135 (MQ=255) cgcgGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCAt < 4:88697/146‑1 (MQ=255) cgcgGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATgcg > 6:63663/1‑149 (MQ=255) gcgGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATgcgc > 7:193279/1‑149 (MQ=255) gcgGGTAAACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATgcgc > 5:307718/1‑149 (MQ=255) gTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGCGCCTGt > 5:293207/1‑149 (MQ=255) | AGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGC‑GCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGG‑CCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGCGCCTGT > NC_002947/4529885‑4530171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |