Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,543,592 C→G 9.0% S9S (TCC→TCG)  rnz → ribonuclease Z

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,543,5920CG 9.0% 227.8 / 11.9 78S9S (TCC→TCG) rnzribonuclease Z
Reads supporting (aligned to +/- strand):  ref base C (36/35);  new base G (3/4);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.99e-01

CAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGA  >  NC_002947/4543448‑4543737
                                                                                                                                                |                                                                                                                                                 
cAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTccgccg                                                                                                                                               >  8:165810/1‑149 (MQ=255)
   cAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCgggg                                                                                                                                            >  4:149490/1‑149 (MQ=255)
    aaGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGt                                                                                                                                           >  1:114094/1‑149 (MQ=255)
       cTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCCCGGCGGAGGTGcc                                                                                                                                        <  3:31614/149‑1 (MQ=255)
          aTCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCccac                                                                                                                                     >  6:262597/1‑149 (MQ=255)
          aTCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCCCGGCGGAGGTGccaa                                                                                                                                      <  5:234494/148‑3 (MQ=255)
              ggCGACAGGATAACTAGCGGGCGGTCGAGGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAg                                                                                                                                 >  1:212113/1‑149 (MQ=255)
               gCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAgg                                                                                                                                <  7:165810/149‑1 (MQ=255)
                cGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGc                                                                                                                               <  2:212113/149‑1 (MQ=255)
                   cAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACg                                                                                                                            >  7:260799/1‑149 (MQ=255)
                      gATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCaa                                                                                                                         >  5:182514/1‑149 (MQ=255)
                         aaCTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTCGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGt                                                                                                                      <  8:310213/149‑1 (MQ=255)
                          aCTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTc                                                                                                                     <  4:141780/149‑1 (MQ=255)
                            tAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAg                                                                                                                   >  4:315144/1‑149 (MQ=255)
                            tAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCAGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAg                                                                                                                   >  3:227054/1‑149 (MQ=255)
                                 ggcggTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCca                                                                                                              >  8:99024/1‑149 (MQ=255)
                                    ggTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGTCCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAg                                                                                                           >  1:189844/1‑149 (MQ=255)
                                    ggTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAg                                                                                                           >  1:42798/1‑149 (MQ=255)
                                    ggTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAg                                                                                                           <  7:59425/149‑1 (MQ=255)
                                        gACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGt                                                                                                       <  7:99024/149‑1 (MQ=255)
                                               ggTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGaa                                                                                                <  2:42798/149‑1 (MQ=255)
                                               ggTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGaa                                                                                                <  2:114094/149‑1 (MQ=255)
                                                     aaTCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGc                                                                                          >  5:42235/1‑149 (MQ=255)
                                                      aTCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCg                                                                                         >  4:76777/1‑149 (MQ=255)
                                                         cGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGaa                                                                                                >  6:86391/1‑139 (MQ=255)
                                                         cGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGaa                                                                                                <  5:86391/139‑1 (MQ=255)
                                                          ggCAAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAg                                                                                     >  3:33315/1‑149 (MQ=255)
                                                             tAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAgc                                                                                                                  <  7:23075/117‑1 (MQ=255)
                                                             tAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAgc                                                                                                                  >  8:23075/1‑117 (MQ=255)
                                                              aaGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAg                                                                                                                                 <  4:80793/101‑1 (MQ=255)
                                                              aaGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAg                                                                                                                                 >  3:80793/1‑101 (MQ=255)
                                                                ggTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTg                                                                               <  3:76777/149‑1 (MQ=255)
                                                                    tgCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTAt                                                                           <  3:149490/149‑1 (MQ=255)
                                                                     gcgcCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATc                                                                          <  4:33315/149‑1 (MQ=255)
                                                                      cgcCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCt                                                                         <  4:108946/149‑1 (MQ=255)
                                                                      cgcCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCt                                                                         <  1:514/149‑1 (MQ=255)
                                                                          ccGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTc                                                                     <  6:42235/149‑1 (MQ=255)
                                                                             tATCACCTTGGCCGCTTCAGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGAt                                                                  <  4:227054/149‑1 (MQ=255)
                                                                               tCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGAtt                                                                 <  8:260799/148‑1 (MQ=255)
                                                                                 aCCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCCCCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTgcg                                                              >  3:15272/1‑149 (MQ=255)
                                                                                  ccTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCTGCCACTGGTATCTGGTCGATTgcgg                                                             >  2:120071/1‑149 (MQ=255)
                                                                                       gCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAg                                                        <  3:315144/149‑1 (MQ=255)
                                                                                        ccGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAgg                                                       >  1:358085/1‑149 (MQ=255)
                                                                                           cTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCAc                                                    <  3:124417/149‑1 (MQ=255)
                                                                                                ggCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTa                                                                            <  4:123659/120‑1 (MQ=255)
                                                                                                ggCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTa                                                                            >  3:123659/1‑120 (MQ=255)
                                                                                                 gCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCa                                              <  5:262597/149‑1 (MQ=255)
                                                                                                   cTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCAcc                                                   >  1:36076/1‑142 (MQ=255)
                                                                                                   cTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCAcc                                                   <  2:36076/142‑1 (MQ=255)
                                                                                                    tGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAAt                                                                                                                        >  6:119592/1‑72 (MQ=255)
                                                                                                    tGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTAt                                                                           >  1:318186/1‑117 (MQ=255)
                                                                                                    tGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTAt                                                                           <  2:318186/117‑1 (MQ=255)
                                                                                                    tGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCg                                           >  5:248225/1‑149 (MQ=255)
                                                                                                     gAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGc                                          >  5:244464/1‑149 (MQ=255)
                                                                                                        aCAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGATGCCAGCGGCAGCCACTGGt                                                                             <  4:338814/111‑1 (MQ=255)
                                                                                                        aCAGGAGCCTGACCATCGACCTGCACTTCCTTGGCACCTCCCCCTGCCTGCCCACCAACGCACGCAATGACAGCGCCACAGCCGTGATCCAAGCCAGCGGCAGCCACtcga                                                                             >  3:338814/1‑108 (MQ=255)
                                                                                                          aGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCCCCCAGCACCGCctgct                                     >  6:268039/1‑149 (MQ=255)
                                                                                                                     cATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGc                          >  7:347427/1‑149 (MQ=255)
                                                                                                                         gACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTc                      <  6:244464/149‑1 (MQ=255)
                                                                                                                           ccTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGa                    >  3:340316/1‑149 (MQ=255)
                                                                                                                            ctgctgTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGATGCCAGCGGCTGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGAt                   <  1:120071/149‑1 (MQ=255)
                                                                                                                            ctgctgTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGcc                                         >  2:236945/1‑127 (MQ=255)
                                                                                                                            ctgctgTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGcc                                         <  1:236945/127‑1 (MQ=255)
                                                                                                                              gctgTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTg                                                                        <  4:364275/94‑1 (MQ=255)
                                                                                                                              gctgTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTg                                                                        >  3:364275/1‑94 (MQ=255)
                                                                                                                               ctgTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGGCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATc                  <  6:182514/147‑1 (MQ=255)
                                                                                                                                  ttggtgggCACCCCGGCGGAGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCctgct                                     <  1:111125/119‑1 (MQ=255)
                                                                                                                                  ttggtgggCACCCCGGCGGAGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCctgct                                     >  2:111125/7‑125 (MQ=255)
                                                                                                                                    ggtgggCACCCCGGCGGAGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGAcc           >  3:107661/5‑149 (MQ=255)
                                                                                                                                     gtgggCACCCCGGCGGAGGTGCCAACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCcgc               <  1:194399/141‑1 (MQ=255)
                                                                                                                                     gtgggCACCCCGGCGGAGGTGCCAACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCcgc               >  2:194399/4‑144 (MQ=255)
                                                                                                                                      ttGGCACCTCCGCCGGGGTGCCCACCAAGTCACGCTATGTCTGCGCCACAGCCGTGATCGTAGAGATCGGCAGCAACTGGTATGTGGTCGATTGCGGCTaa                                                         <  2:15891/101‑1 (MQ=255)
                                                                                                                                      ttGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGaa                                                         >  1:15891/1‑101 (MQ=255)
                                                                                                                                        ggCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTAGATTGCGGCGAAGGCACCCATCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgc        <  2:358085/148‑1 (MQ=255)
                                                                                                                                             cTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgcgcg     >  7:294696/1‑146 (MQ=255)
                                                                                                                                             cTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgcgcg     <  8:294696/146‑1 (MQ=255)
                                                                                                                                             cTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGa  >  7:12384/1‑149 (MQ=255)
                                                                                                                                              tCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATcc                 >  6:49682/1‑133 (MQ=255)
                                                                                                                                              tCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATcc                 <  5:49682/133‑1 (MQ=255)
                                                                                                                                               ccgccgGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCctgc                                      <  4:269918/111‑1 (MQ=255)
                                                                                                                                               ccgccgGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCctgc                                      >  3:269918/1‑111 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGA  >  NC_002947/4543448‑4543737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑