| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,552,024 | C→A | 14.3% | intergenic (+118/+63) | pydA → / ← rutR | NADP‑dependent dihydropyrimidine dehydrogenase subunit PreA/transcriptional regulator, TetR family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,552,024 | 0 | C | A | 14.3% | 71.7 / 5.3 | 28 | intergenic (+118/+63) | pydA/rutR | NADP‑dependent dihydropyrimidine dehydrogenase subunit PreA/transcriptional regulator, TetR family |
| Reads supporting (aligned to +/- strand): ref base C (14/10); new base A (2/2); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01 | |||||||||||
GCGTAACCCCTACCGTGAGGCCAGTTGAAGCGGGTCACCGCCTGAACAGCTGACGCGGCCCCTGTGGGAGCGGCCTTGTGTCGCGATCGGGCCGCACAGCGGCCCCAGCAGTCTTTGCCGCGATACAGACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCGGATCACGGCTCCAGCCCGATCCCCCGCAAGATCACGCTGGTCACCGTCTGCACCGCATTCTCGAAGGCCATGTCAGACAGCACCTCG > NC_002947/4551879‑4552171 | gcgTAACCCCTACCGTGAGGCCAGTTGAAGCGGGTCACCGCCTGAACAGCTGACGCGGCCCCTGTGGGAGCGGCCTTGTGTCGCGATCGGGCCGCACAGCGGCCCCAGCAGTCTTTGCCGCGATACAGACATCGAGGGGCCGCTTCgcg > 1:111678‑M2/1‑146 (MQ=255) cgTAACCCCTACCGTGAGGCCAGTTGAAGCGGGTCACCGCCTGAACAGCTGACGCGGCCCCTGTGGGAGCGGCCTTGTGTCGCGATCGGGCCGCACAGCGGCCCCAGCAGTCTTTGCCGCGATACAGACATCGAGGGGCCGCTTCgcgg > 7:42723‑M2/1‑145 (MQ=255) taagcgcTACGGTGCGGCACGTTGAAGCGGGTCAGCGCCTGAACAGCTGACGCGGCCCCTGTGGGAGGGGCCTTGTGTCGCGATCGGGCCGCACAGCGGCCCCAGCAGTCTTTGCCGCGTTACAGACATCGAGGGGCCGCTTCGCGGcc < 7:292508/143‑1 (MQ=38) tCGGGCCGCACAGCGGCCCCAGCAGTCTTTGCCGCGATACAGACATCGAGGGGCCGCTTCgcggcccgatcgcgacacaaggccgctcccacaggggccgcgtcagctgttcaggcggtgacccgcttcaacaggcctcacggaagggg < 8:54124‑M2/149‑90 (MQ=255) gggCCGCACAGCGGCCCCAGCAGTCTTTGCCGCGATACAGACATCGAGGGGCCGCTTCgcggcccgatcgcgacacaaggccgctcccacaggggccgcgtcagctgttcaggcggtgacccgcttcaactggcctcacggtaggggt < 3:307190‑M2/148‑91 (MQ=255) ggCCGCACAGCGGCCCCAGCAGTCTTTGCCGCGATACAGACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCGGATCACGGCTCCAGCCCGATCCCCCGc > 8:233865/1‑144 (MQ=255) acaGCGGCCCCAGCAGTCTTTGCCGCGATACAGACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCGGATCACGGCTCCAGCCCGATCCCCCGCAAGATCACGCt < 7:233865/149‑1 (MQ=255) accGGCCACAGCAGTCTTTGCCGCGATACAGACATCGAGGGGCCGCTTCCCGGCCCCATCGCGACACAAGGCCCCTCCCACAAAAAGACCACGTCCACCTGGAATCCCGGATCACGGCTCCAGCCCGATCCCCCACAAGAACACGCAgg > 8:115456/3‑149 (MQ=255) aGCGGCCCCAGCAGTCTTTGCCGCGATACAGACATCGAGGGGCCGCTTCgcggcccgatcgcgacacaaggccgctcccacaggggccgcgtcagctgttcaggcggtgacccgcttcaactggcctcacggtaggggttacgcggatc > 4:307190‑M2/1‑49 (MQ=255) gcagTCTTTGCCGCGATACAGACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCGGATCACg < 5:55757/106‑1 (MQ=255) gcagTCTTTGCCGCGATACAGACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCGGATCACg > 6:55757/1‑106 (MQ=255) cagTCTTTGCCGCGATACAGACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCGGATCACGGCTCCCGCCCGATCCCCCGCAAGATCACGCTGGTCACCGTCTg > 1:291875/1‑148 (MQ=255) gCCGCGATACAGACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGAcc < 4:57215/71‑1 (MQ=255) gCCGCGATACAGACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCGGATCACGGCTCCAGCCCGATCCCCCGCAAGATCACGCTGGTCACCGTCTGCAcc > 1:204504/1‑144 (MQ=255) gCCGCGATACAGACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCGGATCACGGCTCCAGCCCGATCCCCCGCAAGATCACGCTGGTCACCGTCTGCAcc < 2:204504/144‑1 (MQ=255) aGACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCGGATCACGGCTCCAGCCCGATCCCCCGCAAGATCACGCTGGTCACCGTCTGCACCGCATTCTCGAAGGcc > 4:175284/1‑149 (MQ=255) aGACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCGGATCACGGCTCCAGCCCGATCCCCCGCAAGATCACGCTGGTCACCGTCTGCACCGCATTCTCGAAGGc > 3:327604/1‑148 (MQ=255) gACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCGGATc > 7:141080/1‑83 (MQ=255) gACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCGGATc < 8:141080/83‑1 (MQ=255) gACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCGGATCACGGCTCCAGCCCGATCCCCCGCAAGATCACGCTGGTCACCGTCTGCACCGCATTCTCGAAGGCCa > 7:73210/1‑149 (MQ=255) aTCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACAACGTCCACCTGGAATGCAGGATCACGGCTCCAGCCCGATCCCACGCAAGATCACGCTGGTCACCGTCTGCACCGCATTCTCGAAGGCCATg > 5:49966/1‑148 (MQ=255) cGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCg > 4:201405/1‑74 (MQ=255) cGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCg < 3:201405/74‑1 (MQ=255) gggCCGCGAAGCGGCCCGATCGCGACACAAGGCCGCTCCCAC‑AAAAGACCACGTCCACCTGGAATGCCGGATCACGGCTCCAGCCCGATCCCCCGCAAGATCACGCTGGTCACCGTCTGCACCGCATTCTCGAAgg > 6:135765/1‑136 (MQ=39) gggCCGCGAAGCGGCCCGATCGCGACACAAGGCCGCTCCCAC‑AAAAGACCACGTCCACCTGGAATGCCGGATCACGGCTCCAGCCCGATCCCCCGCAAGATCACGCTGGTCACCGTCTGCACCGCATTCTCGAAgg < 5:135765/136‑1 (MQ=39) ggCCGCACAgcggcccgatcgcgacacaaggccgctcccacaggggccgcgtcagctgttcaggcggtgacccgcttcaactggcctcacggtaggggttacgcggatcctgcg > 5:126991‑M2/1‑9 (MQ=255) ggCCGCACAgcggcccgatcgcgacacaaggccgctcccacaggggccgcgtcagctgttcaggcggtgacccgcttcaactggcctcacggtaggggttacgcggatcctgcg < 6:126991‑M2/114‑106 (MQ=255) cggttCGCGGCCCGATCGCGACTCAAGGCCGCTCCCACAAAATGACCACGTCCACCTGGAATGCCGGATCACGGCTCCAGCCCGATCCCCCGCAAGATCACGCTGGTCACCGTCTGCACGGCATTCTCGAAGGCCATGTCAGACAGCAc < 7:115456/146‑1 (MQ=255) cgcggcccgatcgcgacacaaggccgctcccacaggggccgcgtcagctgttcaggcggggacccgcttcaactggcctcacggtaggggttacgcggatcctgcgtccagttcaggtacggcttgccggtatcctgtgcctccattt > 7:54124‑M2/1‑1 (MQ=255) | GCGTAACCCCTACCGTGAGGCCAGTTGAAGCGGGTCACCGCCTGAACAGCTGACGCGGCCCCTGTGGGAGCGGCCTTGTGTCGCGATCGGGCCGCACAGCGGCCCCAGCAGTCTTTGCCGCGATACAGACATCGAGGGGCCGCTTCGCGGCCCGATCGCGACACAAGGCCGCTCCCACAAAAAGACCACGTCCACCTGGAATGCCGGATCACGGCTCCAGCCCGATCCCCCGCAAGATCACGCTGGTCACCGTCTGCACCGCATTCTCGAAGGCCATGTCAGACAGCACCTCG > NC_002947/4551879‑4552171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |