Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,654,878	G→A	9.1%	intergenic (+30/‑228)	aceA → / → PP_4118	isocitrate lyase/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,654,878	0	G	A	9.1%	187.1 / 6.7	66	intergenic (+30/‑228)	aceA/PP_4118	isocitrate lyase/membrane protein
Reads supporting (aligned to +/- strand):  ref base G (25/35);  new base A (3/3);  total (28/38)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-02

GGCGCACCAGCAGGAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGC  >  NC_002947/4654731‑4655024                                                                                                                                                    |                                                                                                                                                   ggCGCACCAGCAGGAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCgggg                                                                                                                                                   >  3:333800/1‑149 (MQ=255)    gcACCAGCAGGAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCtt                                                                                                                                                <  6:125136/149‑1 (MQ=255)      aCCAGCAGGAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGTCGCTTCGTCGGTGACTGCGCTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTtgt                                                                                                                                              <  7:51116/149‑1 (MQ=255)      aCCAGCAGGAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTtgt                                                                                                                                              <  4:130576/149‑1 (MQ=255)      aCCAGCAGGAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTtgt                                                                                                                                              >  3:130576/1‑149 (MQ=255)           cagGAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAg                                                                                                                                         >  7:363177/1‑149 (MQ=255)             ggAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTtgt                                                                                                                                              >  7:118389/1‑142 (MQ=255)             ggAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTtgt                                                                                                                                              <  8:118389/142‑1 (MQ=255)             ggAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCt                                                                                                                                       >  3:83032/1‑149 (MQ=255)               aaGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTc                                                                                                                                      <  8:132039/148‑1 (MQ=255)                aGTGGGCACTGGCTACTTCGACGACATGACGACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAATAAGAGCAGTTCCAGTGATAGTGTGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCg                                                                                                                                    <  3:241456/149‑1 (MQ=255)                 gTGGCCACTGGCTACATCGACGACATGAACACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCgg                                                                                                                                   <  6:240160/149‑1 (MQ=255)                       aCTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCttt                                                                                                                             <  4:174885/149‑1 (MQ=255)                              aCTTCCACGACATCACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTa                                                                                                                      <  5:316800/149‑1 (MQ=255)                               cTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACAGACCGCTTCGACCGAAGAAGAGCAGTTCCACTGATAGAGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTTAAAGCTCCGGGCCTTTGTTTTTAc                                                                                                                     >  7:354292/1‑149 (MQ=255)                                   gacgacATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCga                                                                                                                 <  4:254380/149‑1 (MQ=255)                                        cATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAg                                                                                                            >  2:233554/1‑149 (MQ=255)                                         aTGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAgg                                                                                                           >  8:61221/1‑149 (MQ=255)                                         aTGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAgg                                                                                                           <  3:186722/149‑1 (MQ=255)                                           gACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGtt                                                                                                                          <  3:242281/132‑1 (MQ=255)                                           gACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGtt                                                                                                                          >  4:242281/1‑132 (MQ=255)                                                ccGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAgggccgggc                                                                                                    <  4:83032/149‑1 (MQ=255)                                                       ccAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGc                                                                                                                                        >  3:357900/1‑106 (MQ=255)                                                       ccAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGc                                                                                                                                        <  4:357900/106‑1 (MQ=255)                                                         aGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGc                                                                                            >  5:13097/1‑148 (MQ=255)                                                            gcggcgCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAAt                                                                                        <  2:267906/149‑1 (MQ=255)                                                             cggcgCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAAtt                                                                                       <  8:363177/149‑1 (MQ=255)                                                              ggcgCTTCGTCGGTGCCTGCACTGTCCGTTTCGACCTAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACTAATGCCCTGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGACAGGGCCTGGCCTGCTGGCCAAttt                                                                                      <  8:354292/149‑1 (MQ=255)                                                                cgcTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAAtttt                                                                                     >  6:258383/1‑148 (MQ=255)                                                                  cTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTAc                                                                                  <  1:233554/149‑1 (MQ=255)                                                                       tcgGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGcc                                                                             >  4:24263/1‑149 (MQ=255)                                                                          gTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGtt                                                                          <  6:13097/149‑1 (MQ=255)                                                                                    tGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTgg                                                                                             <  1:322700/120‑1 (MQ=255)                                                                                    tGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTgg                                                                                             >  2:322700/1‑120 (MQ=255)                                                                                      aCCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTcc                                                                <  5:258383/147‑1 (MQ=255)                                                                                              gACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTAcc                                                       <  7:61221/148‑1 (MQ=255)                                                                                               aCCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGt                                                                                                                           >  4:3746/1‑79 (MQ=255)                                                                                               aCCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGt                                                                                                                           <  3:3746/79‑1 (MQ=255)                                                                                                ccGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCga                                                                                                                 >  5:389598/1‑88 (MQ=255)                                                                                                ccGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCga                                                                                                                 <  6:389598/88‑1 (MQ=255)                                                                                                 cGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTAt                                                   >  2:115710/1‑149 (MQ=255)                                                                                                   aagaagAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTc                                                 >  2:168224/1‑149 (MQ=255)                                                                                                     gaagaGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTcgc                                               <  4:216128/149‑1 (MQ=255)                                                                                                      aagaGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTcgcg                                              <  4:333800/149‑1 (MQ=255)                                                                                                          gCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTa                                          <  3:24263/149‑1 (MQ=255)                                                                                                            aGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGAGCTTTCACAAGCCCCGGGCCttt                                                                                                                             <  8:292946/64‑1 (MQ=14)                                                                                                            aGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGAGCTTTCACAAGCCCCGGGCCttt                                                                                                                             >  7:292946/1‑64 (MQ=14)                                                                                                             gTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAAc                                       >  7:146138/1‑149 (MQ=255)                                                                                                                 cACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCg                                    >  3:267715/1‑148 (MQ=255)                                                                                                                  aCTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCa                                  <  1:19824/149‑1 (MQ=255)                                                                                                                  aCTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCa                                  >  6:118733/1‑149 (MQ=255)                                                                                                                              ttGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATc                      <  1:168224/149‑1 (MQ=255)                                                                                                                                   gTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCTGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGggag                 <  5:234596/149‑1 (MQ=255)                                                                                                                                    ttGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGggag                 >  6:358564/1‑148 (MQ=255)                                                                                                                                      gAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGggag                 >  4:177728/1‑146 (MQ=255)                                                                                                                                       aaCAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGgaga             <  3:177728/149‑1 (MQ=255)                                                                                                                                       aaCAAAGGCCCGGAGCTTTCACAAGCCCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCa                                                                        <  7:328712/90‑1 (MQ=21)                                                                                                                                       aaCAAAGGCCCGGAGCTTTCACAAGCCCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCa                                                                        >  8:328712/1‑90 (MQ=21)                                                                                                                                        aCAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGac            <  3:298128/149‑1 (MQ=255)                                                                                                                                        aCAAAGGCCCGGAGCTTTCACAAGCCCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCgg                                             >  7:319315/1‑116 (MQ=21)                                                                                                                                        aCAAAGGCCCGGAGCTTTCACAAGCCCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCgg                                             <  8:319315/116‑1 (MQ=21)                                                                                                                                         cAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTc                                                                 >  2:335931/1‑95 (MQ=255)                                                                                                                                         cAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTc                                                                 <  1:335931/95‑1 (MQ=255)                                                                                                                                          aaaGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGaca           >  7:205309/1‑148 (MQ=255)                                                                                                                                           aaGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGttt                                                                  >  2:355205/1‑92 (MQ=255)                                                                                                                                           aaGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGttt                                                                  <  1:355205/92‑1 (MQ=255)                                                                                                                                                ccGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCg    <  8:205309/149‑1 (MQ=255)                                                                                                                                                   gggCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCAGCTACCGTATTCGCGGGTAAACCCGCAACTACACTGATCGGAAGCAGACACACATCGGc  >  6:98698/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GGCGCACCAGCAGGAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGC  >  NC_002947/4654731‑4655024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑