| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,654,891 | T→C | 9.8% | intergenic (+43/‑215) | aceA → / → PP_4118 | isocitrate lyase/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,654,891 | 0 | T | C | 9.8% | 167.9 / 8.1 | 61 | intergenic (+43/‑215) | aceA/PP_4118 | isocitrate lyase/membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (23/32); new base C (3/3); total (26/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.77e-01 | |||||||||||
GGAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGCGATTGGGCGCAGGC > NC_002947/4654743‑4655038 | ggAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCt > 3:83032/1‑149 (MQ=255) aaGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTc < 8:132039/148‑1 (MQ=255) aGTGGGCACTGGCTACTTCGACGACATGACGACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAATAAGAGCAGTTCCAGTGATAGTGTGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCg < 3:241456/149‑1 (MQ=255) gTGGCCACTGGCTACATCGACGACATGAACACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCgg < 6:240160/149‑1 (MQ=255) aCTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCttt < 4:174885/149‑1 (MQ=255) aCTTCCACGACATCACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTa < 5:316800/149‑1 (MQ=255) cTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACAGACCGCTTCGACCGAAGAAGAGCAGTTCCACTGATAGAGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTTAAAGCTCCGGGCCTTTGTTTTTAc > 7:354292/1‑149 (MQ=255) gacgacATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCga < 4:254380/149‑1 (MQ=255) cATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAg > 2:233554/1‑149 (MQ=255) aTGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAgg < 3:186722/149‑1 (MQ=255) aTGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAgg > 8:61221/1‑149 (MQ=255) gACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGtt > 4:242281/1‑132 (MQ=255) gACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGtt < 3:242281/132‑1 (MQ=255) ccGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAgggccgggc < 4:83032/149‑1 (MQ=255) aGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGc > 5:13097/1‑148 (MQ=255) gcggcgCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAAt < 2:267906/149‑1 (MQ=255) cggcgCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAAtt < 8:363177/149‑1 (MQ=255) ggcgCTTCGTCGGTGCCTGCACTGTCCGTTTCGACCTAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACTAATGCCCTGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGACAGGGCCTGGCCTGCTGGCCAAttt < 8:354292/149‑1 (MQ=255) cgcTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAAtttt > 6:258383/1‑148 (MQ=255) cTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTAc < 1:233554/149‑1 (MQ=255) tcgGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGcc > 4:24263/1‑149 (MQ=255) gTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGtt < 6:13097/149‑1 (MQ=255) tGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTgg > 2:322700/1‑120 (MQ=255) tGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTgg < 1:322700/120‑1 (MQ=255) aCCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTcc < 5:258383/147‑1 (MQ=255) gACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTAcc < 7:61221/148‑1 (MQ=255) aCCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGt < 3:3746/79‑1 (MQ=255) aCCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGt > 4:3746/1‑79 (MQ=255) ccGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCga < 6:389598/88‑1 (MQ=255) ccGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCga > 5:389598/1‑88 (MQ=255) cGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTAt > 2:115710/1‑149 (MQ=255) aagaagAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTc > 2:168224/1‑149 (MQ=255) gaagaGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTcgc < 4:216128/149‑1 (MQ=255) aagaGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTcgcg < 4:333800/149‑1 (MQ=255) gCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTa < 3:24263/149‑1 (MQ=255) aGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGAGCTTTCACAAGCCCCGGGCCttt > 7:292946/1‑64 (MQ=14) aGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGAGCTTTCACAAGCCCCGGGCCttt < 8:292946/64‑1 (MQ=14) gTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAAc > 7:146138/1‑149 (MQ=255) cACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCg > 3:267715/1‑148 (MQ=255) aCTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCa > 6:118733/1‑149 (MQ=255) aCTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCa < 1:19824/149‑1 (MQ=255) ttGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATc < 1:168224/149‑1 (MQ=255) gTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCTGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGggag < 5:234596/149‑1 (MQ=255) ttGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGggag > 6:358564/1‑148 (MQ=255) gAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGggag > 4:177728/1‑146 (MQ=255) aaCAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGgaga < 3:177728/149‑1 (MQ=255) aaCAAAGGCCCGGAGCTTTCACAAGCCCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCa < 7:328712/90‑1 (MQ=21) aaCAAAGGCCCGGAGCTTTCACAAGCCCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCa > 8:328712/1‑90 (MQ=21) aCAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGac < 3:298128/149‑1 (MQ=255) aCAAAGGCCCGGAGCTTTCACAAGCCCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCgg > 7:319315/1‑116 (MQ=21) aCAAAGGCCCGGAGCTTTCACAAGCCCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCgg < 8:319315/116‑1 (MQ=21) cAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTc < 1:335931/95‑1 (MQ=255) cAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTc > 2:335931/1‑95 (MQ=255) aaaGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGaca > 7:205309/1‑148 (MQ=255) aaGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGttt > 2:355205/1‑92 (MQ=255) aaGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGttt < 1:355205/92‑1 (MQ=255) ccGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCg < 8:205309/149‑1 (MQ=255) gggCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCAGCTACCGTATTCGCGGGTAAACCCGCAACTACACTGATCGGAAGCAGACACACATCGGc > 6:98698/1‑148 (MQ=255) cTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGCGAtt < 8:146138/149‑1 (MQ=255) ttGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGCGATTg > 8:6764/1‑149 (MQ=255) tgtgAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGCGATTgg > 6:341227/1‑149 (MQ=255) cTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGCGATTGGGCGCAGGc < 5:118733/149‑1 (MQ=255) | GGAAGTGGGCACTGGCTACTTCGACGACATGACCACCGTGATCCAGGGCGGCGCTTCGTCGGTGACTGCACTGACCGGTTCGACCGAAGAAGAGCAGTTCCACTGATAGTGGGTTGCCGTTGAACAAAGGCCCGGGGCTTGTGAAAGCTCCGGGCCTTTGTTTTTACCCGAGAAAGGGCCGGGCCTGCTGGCCAATTTTTACCAGCCGTTCACCGTTTCCGCCGCTACCGTATTCGCGGGTAAACCCGCACCTACACTGATCGGGAGGAGACACACATCGGCGATTGGGCGCAGGC > NC_002947/4654743‑4655038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |