Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,665,001 C→G 7.0% R45R (CGC→CGG)  nuoI → NADH‑quinone oxidoreductase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,665,0010CG 7.0% 224.4 / 4.9 69R45R (CGC→CGG) nuoINADH‑quinone oxidoreductase subunit I
Reads supporting (aligned to +/- strand):  ref base C (31/33);  new base G (3/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTGACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACC  >  NC_002947/4664858‑4665137
                                                                                                                                               |                                                                                                                                        
aTTTGACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGGTAGcg                                                                                                                                     >  1:299654/1‑149 (MQ=255)
   tGACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACcgcg                                                                                                                                   <  4:197668/148‑1 (MQ=255)
   tGACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACcgcg                                                                                                                                   <  3:175459/148‑1 (MQ=255)
          tGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATc                                                                                                                           <  8:296466/149‑1 (MQ=255)
            ttCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCGGCCGCGGTAGCGCGGCGGCaggta                                                                                                                         >  7:98710/1‑145 (MQ=255)
              cAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGGGTTCTCCCACGGGTTCCGCCAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTc                                                                                                                        <  5:299361/148‑1 (MQ=255)
               aaGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCGGCCGCGGTAGCGCGGCGGCaggtacac                                                                                                                      <  8:98710/149‑8 (MQ=255)
                           gACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCTCGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGAccc                                                                                                          <  7:48275/149‑1 (MQ=255)
                           gACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGAccc                                                                                                          <  3:343276/149‑1 (MQ=255)
                               tCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGGCCCCGAt                                                                                                      >  5:316781/1‑149 (MQ=255)
                                cGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATg                                                                                                     >  5:4929/1‑149 (MQ=255)
                                 gTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATgg                                                                                                    >  3:244072/1‑149 (MQ=255)
                                    aaGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGc                                                                                                   >  4:62949/1‑147 (MQ=255)
                                       ggcaccggcaccCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATCCCCCGAAGAACCCGTGTACCTGCCGCCGGGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCgagga                                                                                              <  2:71930/149‑1 (MQ=255)
                                         caccggcaccCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAgc                                                                                            <  5:93726/149‑1 (MQ=255)
                                          accggcaccCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAgcg                                                                                           >  5:187075/1‑149 (MQ=255)
                                            cggcaccCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCt                                                                                         <  2:228805/149‑1 (MQ=255)
                                            cggcaccCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCt                                                                                         <  6:69855/149‑1 (MQ=255)
                                                accCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCg                                                                                      >  7:49121/1‑148 (MQ=255)
                                                   cAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGGCCCCGATGGCGAGGAGCGCTGCGTAGc                                                                                  <  6:316781/149‑1 (MQ=255)
                                                     gCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGt                                                                                <  6:4929/149‑1 (MQ=255)
                                                            aGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAAcc                                                                          >  6:21085/1‑148 (MQ=255)
                                                                  gCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTgcgc                                                                   >  7:106546/1‑149 (MQ=255)
                                                                    aaTGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATc                                                                                                                           >  6:244151/1‑91 (MQ=255)
                                                                    aaTGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATc                                                                                                                           <  5:244151/91‑1 (MQ=255)
                                                                          gtTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCt                                                           >  2:57289/1‑149 (MQ=255)
                                                                           ttCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCt                                                           <  8:49121/148‑1 (MQ=255)
                                                                              tcCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGccc                                                       >  6:25478/1‑149 (MQ=255)
                                                                                ccACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCt                                                                                                                      >  3:275039/1‑84 (MQ=255)
                                                                                ccACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCt                                                                                                                      <  4:275039/84‑1 (MQ=255)
                                                                                ccACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCgg                                                     >  1:318879/1‑149 (MQ=255)
                                                                                  aCGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTg                                                   <  8:106546/149‑1 (MQ=255)
                                                                                    gggTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTggg                                                 <  2:318879/149‑1 (MQ=255)
                                                                                       ttCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTg                                              <  5:25478/149‑1 (MQ=255)
                                                                                         ccGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGc                                                             <  8:90938/132‑1 (MQ=255)
                                                                                         ccGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGc                                                             >  7:90938/1‑132 (MQ=255)
                                                                                          cGCAAGCGCGACACCCTGCAATCCCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGt                                                                <  5:172271/128‑1 (MQ=255)
                                                                                          cGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGt                                                                >  6:172271/1‑128 (MQ=255)
                                                                                          cGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCAt                                           >  6:170618/1‑149 (MQ=255)
                                                                                                 gcgACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTg                                    <  6:187075/149‑1 (MQ=255)
                                                                                                        ccTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCgag                                                                                                <  1:229933/82‑1 (MQ=255)
                                                                                                        ccTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCgag                                                                                                >  2:229933/1‑82 (MQ=255)
                                                                                                               tACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCgaga                       >  5:9029/1‑148 (MQ=255)
                                                                                                                  cccGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCgag                                                                                                >  4:304823/1‑72 (MQ=255)
                                                                                                                  cccGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCgag                                                                                                <  3:304823/72‑1 (MQ=255)
                                                                                                                  cccGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCg                          <  5:371221/142‑1 (MQ=255)
                                                                                                                  cccGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCg                          >  6:371221/1‑142 (MQ=255)
                                                                                                                  cccGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACa                     >  2:362034/1‑147 (MQ=255)
                                                                                                                   ccGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACagag                  >  3:277105/1‑149 (MQ=255)
                                                                                                                    cGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGc                            >  8:90372/1‑138 (MQ=255)
                                                                                                                    cGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGc                            <  7:90372/138‑1 (MQ=255)
                                                                                                                    cGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACagag                  >  1:281622/1‑148 (MQ=255)
                                                                                                                    cGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACagag                  >  8:204878/1‑148 (MQ=255)
                                                                                                                     gaagaaCCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTgcgc                                                                   <  5:141135/98‑1 (MQ=255)
                                                                                                                     gaagaaCCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTgcgc                                                                   >  6:141135/1‑98 (MQ=255)
                                                                                                                      aagaaCCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGAc               >  7:211294/1‑149 (MQ=255)
                                                                                                                         aaCCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTgc                                                                     >  2:280422/1‑92 (MQ=255)
                                                                                                                         aaCCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGa                <  7:195450/145‑1 (MQ=255)
                                                                                                                         aaCCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGa                >  8:195450/1‑145 (MQ=255)
                                                                                                                            ccGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGcc                           <  4:176169/131‑1 (MQ=255)
                                                                                                                            ccGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGcc                           >  3:176169/1‑131 (MQ=255)
                                                                                                                            ccGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGc         <  4:244072/149‑1 (MQ=255)
                                                                                                                            ccGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGc         <  2:281622/149‑1 (MQ=255)
                                                                                                                              gtgtACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTg       <  7:204878/149‑1 (MQ=255)
                                                                                                                                   ccTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTAcc  <  1:57289/149‑1 (MQ=255)
                                                                                                                                         gccgcGGTAGCGCGGCGGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTg       <  7:273789/138‑1 (MQ=255)
                                                                                                                                         gccgcGGTAGCGCGGCGGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTg       >  8:273789/1‑138 (MQ=255)
                                                                                                                                           cgcgCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTg                                                   <  6:78236/92‑1 (MQ=255)
                                                                                                                                           cgcgCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTg                                                   >  5:78236/1‑92 (MQ=255)
                                                                                                                                               |                                                                                                                                        
ATTTGACCCATGTTCAAGTATATCGGCGACATCGTTAAGGGCACCGGCACCCAGCTGCGCAGCCTGGCAATGGTGTTCTCCCACGGGTTCCGCAAGCGCGACACCCTGCAATACCCCGAAGAACCCGTGTACCTGCCGCCGCGCTACCGCGGCCGCATCGTCCTCACCCGCGACCCCGATGGCGAGGAGCGCTGCGTAGCGTGCAACCTCTGCGCGGTGGCCTGCCCGGTGGGCTGCATTTCGCTGCAGAAGGCCGAGACAGAGGACGGCCGCTGGTACC  >  NC_002947/4664858‑4665137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑