Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,726,926 C→G 10.4% P152P (CCG→CCC)  brnQ ← branched‑chain amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,726,9260CG10.4% 139.5 / 6.2 48P152P (CCG→CCC) brnQbranched‑chain amino acid transporter
Reads supporting (aligned to +/- strand):  ref base C (20/23);  new base G (3/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

CACCCAGGGTATCCATGGTCAGGTAGCCATCAGAGAAGCCCTTGGAGAACGCAGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCACCGGTCAGCGGTGCAACGCCCACTTCGAACGACACGG  >  NC_002947/4726781‑4727074
                                                                                                                                                 |                                                                                                                                                    
cACCCAGGGTATCCATGGTCAGGTAGCCATCAGAGAAGCCCTTGGAGAACGCAGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGt                                                                                                                                                   <  7:132677/149‑1 (MQ=255)
 aCCCAGGGTATCCATGGTCAGGTAGCCATCAGAGAAGCCCTTGGAGAACGCAGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTg                                                                                                                                                  >  3:243454/1‑149 (MQ=255)
  cccAGGGTATCCATGGTCAGGTAGCCATCAGAGAAGCCCTTGGAGAACGCAGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGc                                                                                                                                                 <  2:166794/149‑1 (MQ=255)
           tCCATGGTCAGGTAGCCATCAGAGAAGCCCTTGGAGAACGCAGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCg                                                                                                                                        <  5:63044/149‑1 (MQ=255)
                    aGGTAGCCATCAGAGAAGCCCTTGGAGAACGCAGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAAc                                                                                                                                                      <  8:128352/126‑1 (MQ=255)
                    aGGTAGCCATCAGAGAAGCCCTTGGAGAACGCAGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAAc                                                                                                                                                      >  7:128352/1‑126 (MQ=255)
                      gTAGCCATCAGAGAAGCCCTTGGAGAACGCAGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGgtgt                                                                                                                             <  6:361918/149‑1 (MQ=255)
                            aTCAGAGAAGCCCTTGGAGAACGCAGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGc                                                                                                                        >  2:44262/1‑148 (MQ=255)
                             tCAGAGTAGCCCTTGGAGAACGCAGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGGGCGAAGGCAGCGATGCCCAGTACGGCCAGGTCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCat                                                                                                                      <  2:283200/149‑2 (MQ=255)
                                gagaAGCCCTTGGAGAACGCAGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCa                                                                                                                                   <  6:368499/133‑1 (MQ=255)
                                gagaAGCCCTTGGAGAACGCAGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCa                                                                                                                                   >  5:368499/1‑133 (MQ=255)
                                       ccTTGGAGAACGCAGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAggg                                                                                                            <  1:273403/149‑1 (MQ=255)
                                                    aGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACgg                                                                                               >  5:333158/1‑149 (MQ=255)
                                                           gcaTAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCac                                                                                        <  7:319824/149‑1 (MQ=255)
                                                            caTAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAAGGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCac                                                                                        >  5:96800/1‑148 (MQ=255)
                                                            caTAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAAGGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCac                                                                                        >  5:91347/1‑148 (MQ=255)
                                                            caTAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGgtgt                                                                                                                             >  5:361360/1‑111 (MQ=255)
                                                            caTAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGgtgt                                                                                                                             <  6:361360/111‑1 (MQ=255)
                                                                         tgCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTa                                                                            <  3:93219/147‑1 (MQ=255)
                                                                            gTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCa                                                                       <  6:343408/149‑1 (MQ=255)
                                                                               aCCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAAGGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGc                                                                    <  6:96800/149‑1 (MQ=255)
                                                                               aCCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAAGGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGc                                                                    <  6:91347/149‑1 (MQ=255)
                                                                               aCCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGc                                                                    >  8:22414/1‑149 (MQ=255)
                                                                                  aaTGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGt                                                                 <  6:333158/149‑1 (MQ=255)
                                                                                      cTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAgg                                                                                                             >  5:366027/1‑101 (MQ=255)
                                                                                      cTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAgg                                                                                                             <  6:366027/101‑1 (MQ=255)
                                                                                       tACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAgg                                                                     >  4:43068/1‑140 (MQ=255)
                                                                                       tACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAgg                                                                     <  3:43068/140‑1 (MQ=255)
                                                                                       tACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGAt                                                             >  5:264104/1‑148 (MQ=255)
                                                                                         ccGGCGGGCAGCGGGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGaa                                                          >  2:159524/1‑149 (MQ=255)
                                                                                         ccGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGaa                                                          <  7:22414/149‑1 (MQ=255)
                                                                                          cggcggGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCg                                                                                                                           <  8:216404/83‑1 (MQ=255)
                                                                                          cggcggGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCg                                                                                                                           >  7:216404/1‑83 (MQ=255)
                                                                                                     gcgAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAggg                                                                                                            >  4:269741/1‑87 (MQ=255)
                                                                                                     gcgAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAggg                                                                                                            <  3:269741/87‑1 (MQ=255)
                                                                                                             aGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCAc                                      >  8:160524/1‑149 (MQ=255)
                                                                                                              gCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCAcc                                     <  5:26400/149‑1 (MQ=255)
                                                                                                                     ccAGTACGGCCAGGTCAATGATCTTCAACGGTGTCAGGAAGTGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTTTAGATGAACAGCGCCAGCGGGCTTTCACCGGTCa                                <  5:49708/147‑1 (MQ=255)
                                                                                                                     ccAGTACGGCCAGGGCAATGATCTTCAAGGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCACCGGTCAGc                              >  1:216120/1‑149 (MQ=255)
                                                                                                                     ccAGTACGGCCAGGGCAATGATCTTCAACGGTGTCAGGAAGTGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACCGCCAGCACGACGATGAAGTACACCACGCTGTAGAAGAACAGCGCCAGCGGGCTTTCACCGGGCa                                >  6:49708/1‑147 (MQ=255)
                                                                                                                        gTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCGACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCACCGGTCAGCGGt                           <  2:232492/149‑1 (MQ=255)
                                                                                                                        gTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCACCGGTCAGCGGt                           <  8:284026/149‑1 (MQ=255)
                                                                                                                             gCCAGGGCAAAGATCATCACCGGTTCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCACCGCTGACCGGTGaaag                      <  6:264104/149‑2 (MQ=255)
                                                                                                                              ccAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCACCGGTCAGCGGTGCAACg                     >  2:282183/1‑149 (MQ=255)
                                                                                                                               cAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCACCGGTCAGCGGTGCAACGc                    >  4:64521/1‑149 (MQ=255)
                                                                                                                                  ggCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCa                                       >  7:348648/1‑127 (MQ=255)
                                                                                                                                  ggCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCa                                       <  8:348648/127‑1 (MQ=255)
                                                                                                                                     aaTGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCACCGGTCAGCGGTGCAACGCCCACtt              >  7:183624/1‑149 (MQ=255)
                                                                                                                                     aaTGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCACCGGTCAGCGGTGCAACGCCCACtt              >  6:242849/1‑149 (MQ=255)
                                                                                                                                              cAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCACCGGTCAGCGGTGCAACGCCCACTTCGAACGaca     >  6:360991/1‑149 (MQ=255)
                                                                                                                                                 cGGTGCAAGGAAGCGGCCCACGGTTTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATTAAGTACACCAGGCTGTAGATTAACAGCGCCAGCGGGCTTTCACCGGTCAGCTGTGCAACGCCCACTTCGAACGACACgg  <  2:216120/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CACCCAGGGTATCCATGGTCAGGTAGCCATCAGAGAAGCCCTTGGAGAACGCAGCGGCAGCATAGGCAGGCTGTGCGTCACCAATGCTACCGGCGGGCAGCGCGAAGGCAGCGATGCCCAGTACGGCCAGGGCAATGATCTTCAACGGTGCCAGGAAGCGGCCCACGGTGTCGAGCAACTTGCCAGGGTACATGGACACGGCCAGCACGACGATGAAGTACACCAGGCTGTAGATGAACAGCGCCAGCGGGCTTTCACCGGTCAGCGGTGCAACGCCCACTTCGAACGACACGG  >  NC_002947/4726781‑4727074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑