Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,732,495 G→C 5.5% A89G (GCC→GGC)  sucB ← 2‑oxoglutarate dehydrogenase dihydrolipoyltranssuccinylase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,732,4950GC 5.5% 228.4 / 2.6 72A89G (GCC→GGC) sucB2‑oxoglutarate dehydrogenase dihydrolipoyltranssuccinylase subunit
Reads supporting (aligned to +/- strand):  ref base G (36/32);  new base C (2/2);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

ACGCGACCGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACAACCTTGTCAGTCT  >  NC_002947/4732354‑4732642
                                                                                                                                             |                                                                                                                                                    
aCGCGACCGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCcgc                                                                                                                                               >  4:32657/1‑149 (MQ=255)
aCGCGACCGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCcg                                                                                                                                                <  8:54377/148‑1 (MQ=255)
  gcgACCGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCgg                                                                                                                                             >  5:168487/1‑149 (MQ=255)
  gcgACCGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCgg                                                                                                                                             >  8:336862/1‑149 (MQ=255)
    gACCGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGTGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCgg                                                                                                                                             >  6:231816/1‑147 (MQ=255)
     aCCGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAg                                                                                                                                          <  4:73749/149‑1 (MQ=255)
       cGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCCGCTGCGCCTGCCGCGGCAgcc                                                                                                                                        >  3:339189/1‑147 (MQ=255)
       cGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGgc                                                                                                                                        <  3:248081/149‑1 (MQ=255)
       cGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGCCTGCCGCGGCAg                                                                                                                                          <  8:20047/147‑1 (MQ=255)
       cGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGCCTGCCGCGGCAg                                                                                                                                          >  7:20047/1‑147 (MQ=255)
             tACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGc                                                                                                                                   <  7:336862/148‑1 (MQ=255)
                     ccTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCCGCTGCGCCTGCCGCGGCAGCCGCACCTgcagctgc                                                                                                                          <  4:339189/149‑4 (MQ=38)
                           aCGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGTGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTc                                                                                                                    <  5:231816/149‑1 (MQ=255)
                              gTAGCCAGGTCGAGGCCGGTGGCTTCAGCCCGCTTGCGCGCGGCCGGGGCGGCGATCGGGGCATCTTCGCCGGCGGCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAAc                                                                                                                 <  4:49195/149‑1 (MQ=255)
                                   cAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATc                                                                                                             >  7:268712/1‑148 (MQ=255)
                                         gATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAAcc                                                                                                        >  2:259079/1‑147 (MQ=255)
                                         gATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCa                                                                                                      <  8:268712/149‑1 (MQ=255)
                                            gCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGc                                                                                                    >  2:276839/1‑148 (MQ=255)
                                                ttttCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATtc                                                                                               >  4:66329/1‑149 (MQ=255)
                                                    cttcAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGc                                                                                                                                   >  3:251009/1‑109 (MQ=255)
                                                    cttcAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGc                                                                                                                                   <  4:251009/109‑1 (MQ=255)
                                                    cttcAGCCAGCTGGCGCGCGGCCGGGGCGGCGATCGGGTGATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGTGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATtcgtc                                                                                            <  4:64049/148‑1 (MQ=255)
                                                     ttcAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATtcgtcg                                                                                           >  5:164858/1‑148 (MQ=255)
                                                       cagccagcTTGCGCGCGGCGGGGGCTGAGATCGGGTCATCTTCGCCGGGGTCAGCGGCAGCTGGGGCCGGAGCAGGTGCAGGTGGGGGTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATtcgtcg                                                                                           <  8:236535/146‑1 (MQ=255)
                                                            agcTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAg                                                                                                                                          <  8:288479/94‑1 (MQ=255)
                                                            agcTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAg                                                                                                                                          >  7:288479/1‑94 (MQ=255)
                                                             gcTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATtc                                                                                               <  2:334207/136‑1 (MQ=255)
                                                             gcTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATtc                                                                                               >  1:334207/1‑136 (MQ=255)
                                                             gcTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACg                                                                                 <  5:166606/149‑1 (MQ=255)
                                                              cTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACgg                                                                                >  5:362109/1‑149 (MQ=255)
                                                               ttGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGgt                                                                               >  5:106654/1‑149 (MQ=255)
                                                                    cgcgGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGtcgc                                                                          >  4:80697/1‑149 (MQ=255)
                                                                       ggCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGtcg                                                                           >  4:300842/1‑145 (MQ=255)
                                                                       ggCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGtcg                                                                           <  3:300842/145‑1 (MQ=255)
                                                                         ccGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGtcgccctcg                                                                     <  1:259079/149‑1 (MQ=255)
                                                                            gggCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGtcgccctcgccc                                                                  <  3:80697/149‑1 (MQ=255)
                                                                             ggCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCg                                                                                                                         >  6:177773/1‑94 (MQ=255)
                                                                             ggCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCg                                                                                                                         <  5:177773/94‑1 (MQ=255)
                                                                                tGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGa                                                              >  8:87990/1‑149 (MQ=255)
                                                                                 gCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑Ga                                                                                   >  2:195358/1‑127 (MQ=255)
                                                                                 gCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑Ga                                                                                   <  1:195358/127‑1 (MQ=255)
                                                                                  cGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACg                                                            <  6:168487/149‑1 (MQ=255)
                                                                                        ggTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCg                                                      >  8:37572/1‑149 (MQ=255)
                                                                                        ggTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCg                                                      >  6:346911/1‑149 (MQ=255)
                                                                                          tCATCTTCGCCGGCGGCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGcc                                                    <  4:36176/149‑1 (MQ=255)
                                                                                            aTCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGccc                                                   <  3:32657/148‑1 (MQ=255)
                                                                                             tCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAg                                                 >  5:302400/1‑149 (MQ=255)
                                                                                                 cGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGc                                                <  6:164858/146‑1 (MQ=255)
                                                                                                 cGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACg                                             >  4:8223/1‑149 (MQ=255)
                                                                                                        gTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCgg                                      >  3:366724/1‑149 (MQ=255)
                                                                                                        gTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGAAAATCGTCGGACAC‑GACGGTGTCGCCCTCCCCCTTGACGATGTCTCCCAGCACGCCAACgg                                      >  4:245161/1‑149 (MQ=255)
                                                                                                           aGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCa                                    <  6:362109/148‑1 (MQ=255)
                                                                                                           aGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAg                                   >  2:377768/1‑149 (MQ=255)
                                                                                                             gggCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTa                                 <  3:245161/148‑1 (MQ=255)
                                                                                                                cagcagCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGcc                              <  1:8804/149‑1 (MQ=255)
                                                                                                                 agcagcGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGc                               >  6:227146/1‑147 (MQ=255)
                                                                                                                    aggggCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTa                          <  7:107554/146‑1 (MQ=255)
                                                                                                                    agcGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTa                          >  6:86333/1‑149 (MQ=255)
                                                                                                                     gcGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAGCCACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATc                                        <  1:270055/135‑1 (MQ=255)
                                                                                                                     gcGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATc                                        >  2:270055/1‑134 (MQ=255)
                                                                                                                        gCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGATCCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTc                      <  6:302400/149‑1 (MQ=255)
                                                                                                                               cagcTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACa               >  5:144086/1‑149 (MQ=255)
                                                                                                                                    gCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACAACCtt          >  7:266000/1‑149 (MQ=255)
                                                                                                                                     cAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACAACCtt          <  5:86333/148‑1 (MQ=255)
                                                                                                                                      aGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGGCAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACAACCTTg         >  8:151337/1‑148 (MQ=255)
                                                                                                                                      aGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACAACCTTGt        <  6:106654/149‑1 (MQ=255)
                                                                                                                                      aGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACAACCTTGt        >  5:360324/1‑149 (MQ=255)
                                                                                                                                        gTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCg                                       >  4:88469/1‑116 (MQ=255)
                                                                                                                                        gTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCg                                       <  3:88469/116‑1 (MQ=255)
                                                                                                                                        gTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAg                            <  2:128628/127‑1 (MQ=255)
                                                                                                                                        gTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAg                            >  1:128628/1‑127 (MQ=255)
                                                                                                                                          gCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACAACCTTGTCAGt    >  3:19212/1‑149 (MQ=255)
                                                                                                                                            ggCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACAACCTTGTCAGtct  <  5:346911/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
ACGCGACCGCCTTTACCGGTACCTGCAACGGTAGCCAGGTCGATGCCGTTTTCTTCAGCCAGCTTGCGCGCGGCCGGGGCTGCGATCGGGTCATCTTCGCCGGCGTCAGCGGCAGCAGCGGCCGGAGCAGCTGCAGGTGCGGCTGCCGCGGCAGGCGCAGCGGCAGCACCGCCCTCAACGATCGAACCCAGCAATTCGTCGGACAG‑GACGGTGTCGCCCTCGCCCTTGACGATGTCGCCCAGCACGCCATCGGCAGTAGCCAGTACTTCCAGGACAACCTTGTCAGTCT  >  NC_002947/4732354‑4732642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑