Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,738,324	C→T	100%	G16S (GGC→AGC)	sdhA ←	succinate dehydrogenase flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,738,324	0	C	T	100.0%	170.9 / NA	50	G16S (GGC→AGC)	sdhA	succinate dehydrogenase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/29);  total (21/29)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTCGGCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCCACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAATCGCAGTTGCCG  >  NC_002947/4738183‑4738467                                                                                                                                               |                                                                                                                                                 ggTCGGCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCgatg                                                                                                                                           <  7:216927/149‑1 (MQ=255) ggTCGGCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCgatg                                                                                                                                           <  2:299258/149‑1 (MQ=255)  gTCGGCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCACATGCCGGCGCCGCCGCTACCgatga                                                                                                                                          >  8:61832/1‑149 (MQ=255)   tCGGCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCgatgat                                                                                                                                         <  1:178803/149‑1 (MQ=255)   tCGGCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCgatgat                                                                                                                                         >  3:321064/1‑149 (MQ=255)   tCGGCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCgatga                                                                                                                                          >  2:272950/1‑148 (MQ=255)   tCGGCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCgatga                                                                                                                                          <  1:272950/148‑1 (MQ=255)        cGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGTTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCATTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGACGCTACCGATGATGATgg                                                                                                                                    <  4:252521/149‑1 (MQ=255)        cGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATgg                                                                                                                                    >  7:295421/1‑149 (MQ=255)           aGCGAGGGCGCAGGGTATGCCGCCCGGGGCGGATACGGTGGGCGACCGGGTCGGGAAGACCTTGGGGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGt                                                                                                                                 <  4:112292/149‑1 (MQ=255)            gCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTc                                                                                                                                >  4:252489/1‑149 (MQ=255)               aTGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGaa                                                                                                                             <  5:13722/149‑1 (MQ=255)               aTGGCGCAGGGGATGCCGCCCTGGGCGGATACGGTGTGCGATCGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGaa                                                                                                                             <  8:204364/149‑1 (MQ=255)                tGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAg                                                                                                                            <  8:295421/149‑1 (MQ=255)                       ggTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATgg                                                                                                                     <  7:354390/149‑1 (MQ=255)                           aTGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTg                                                                                                                  <  3:137069/148‑1 (MQ=255)                             gccgccCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGaa                                                                                                               >  5:19638/1‑149 (MQ=255)                              ccgccCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAAt                                                                                                              <  5:106203/149‑1 (MQ=255)                                 cccTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCt                                                                                                           <  1:57356/149‑1 (MQ=255)                                      ggCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGc                                                                                                        <  3:166903/147‑1 (MQ=255)                                         ggATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATg                                                                                                    >  8:215672/1‑148 (MQ=255)                                            tACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATc                                                                                                <  6:19638/149‑1 (MQ=255)                                             aCGGTGTGCGATCGGGTCGGGAAGAGCTTGGTGACTAAGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATc                                                                                                <  5:13377/148‑1 (MQ=255)                                                gtgtGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGa                                                                                             >  4:38408/1‑148 (MQ=255)                                                  gtgCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCACATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATAc                                                                                          <  7:61832/149‑1 (MQ=255)                                                    gCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATAccc                                                                                        >  4:290260/1‑149 (MQ=255)                                                       aaCGGGTCGGGAAGACCTTGGTGACTACGGCAGTCGTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATAccc                                                                                        >  1:56516/1‑147 (MQ=255)                                                            gTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAAt                                                                                <  4:321064/149‑1 (MQ=255)                                                             tCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATc                                                                               >  5:211895/1‑149 (MQ=255)                                                               gggCAGACCTGGGTGACTACGGCACTC‑TTGTGGCCGCCTTGAGCCAGGTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTg                                                                             <  4:49171/149‑1 (MQ=255)                                                               gggAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTg                                                                             <  4:222321/149‑1 (MQ=255)                                                                 gAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGca                                                                           <  4:87710/149‑1 (MQ=255)                                                                  aaGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTgg                                                                                                                 >  2:58803/1‑110 (MQ=255)                                                                              agtACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGaa                                                              <  4:59536/147‑1 (MQ=255)                                                                               cTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGaa                                                              <  7:215672/148‑1 (MQ=255)                                                                                    gCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGc                                                       >  2:248772/1‑149 (MQ=255)                                                                                           ttGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TACGCGAACAt                                                 >  2:303800/1‑149 (MQ=255)                                                                                           ttGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACAt                                                 >  2:103385/1‑149 (MQ=255)                                                                                             gtgGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCg                                               >  3:189191/1‑149 (MQ=255)                                                                                                gccgccTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCGCaa                                            >  2:18247/1‑149 (MQ=255)                                                                                                                tGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCGCAACGCCGCATACCGCCTg                            >  1:322942/1‑149 (MQ=255)                                                                                                                 gCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCGCAACGCCGCATACCGCCTg                            <  3:252489/148‑1 (MQ=255)                                                                                                                 gCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCGCAACGCCGCATACCGCCTg                            <  2:56516/148‑1 (MQ=255)                                                                                                                       cTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAgg                     <  3:290260/149‑1 (MQ=255)                                                                                                                         gcgcgcATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCGc                                              >  6:243163/1‑122 (MQ=255)                                                                                                                         gcgcgcATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCGc                                              <  5:243163/122‑1 (MQ=255)                                                                                                                          cgcgcATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGa                    <  6:211895/147‑1 (MQ=255)                                                                                                                              cATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCg                                               <  4:14645/116‑1 (MQ=255)                                                                                                                              cATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCg                                               >  3:14645/1‑116 (MQ=255)                                                                                                                                  ccGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAATCg           >  7:190546/1‑148 (MQ=255)                                                                                                                                         cgccgctACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTTAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAATCGCAGTTGc    <  1:103385/149‑1 (MQ=255)                                                                                                                                           ccgctACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAATCGCAGTTGCCg  >  1:193329/1‑148 (MQ=255)                                                                                                                                               |                                                                                                                                                 GGTCGGCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTC‑TTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCCACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAG‑TAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAATCGCAGTTGCCG  >  NC_002947/4738183‑4738467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑