Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,761,543	C→A	5.4%	P68T (CCC→ACC)	pvdN →	pyoverdine biosynthesis‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,761,543	0	C	A	5.4%	290.9 / 3.9	90	P68T (CCC→ACC)	pvdN	pyoverdine biosynthesis‑like protein
Reads supporting (aligned to +/- strand):  ref base C (39/46);  new base A (2/3);  total (41/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

CCCTTGGCCGGCCAACTGCTGGGCAGCGCCCAGGCGGCGCCGCGCACGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCTTGAAGTCAAGCCACGC  >  NC_002947/4761396‑4761686                                                                                                                                                      |                                                                                                                                                 cccTTGGCCGGCCAACTGCTGGGCAGCGCCCAGGCGGCGCCGCGCCCGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGccc                                                                                                                                                 >  8:118973/1‑149 (MQ=255) cccTTGGCCGGCCAACTGCTGGGCAGCGCCCAGGCGGCGCCGCGCACGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGccc                                                                                                                                                 >  6:145898/1‑149 (MQ=255)        ccggccAACTGCTGGGCAGCGCCCAGGCGGCGCCGCGCACGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTgcgc                                                                                                                                         >  5:94021/1‑149 (MQ=255)                ctgctgGGCAGCGCCCAGGCGGCGCCGCGCACGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGAt                                                                                                                                 <  6:94021/149‑1 (MQ=255)                      ggCAGCGCCCAGGCGGCGCCGCGCACGCGCTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCAC‑GGGGcgcgggtgcg                                                                                                                                  >  1:23136/1‑137 (MQ=255)                      ggCAGCGCCCAGGCGGCGCCGCGCACGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCaa                                                                                                                            <  1:162462/148‑1 (MQ=255)                      ggCAGCGCCCAGGCGGCGCCGCGCACGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAg                                                                                                                           >  8:122647/1‑149 (MQ=255)                      ggCAGCGCCCAGGCGGCGCCGCGCACGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCAC‑GGGGcgcgggtgcg                                                                                                                                  <  2:23136/142‑7 (MQ=255)                      ggCAGCGCCCAGGCGGCGCCGCGCACGC‑CTCCGGCCACGGATGCCTGGAGCGCTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAg                                                                                                                           >  4:165861/1‑149 (MQ=255)                       gCAGCGCCCAGGCGGCGCCGCGCACGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGc                                                                                                                          <  3:373203/149‑1 (MQ=255)                       gCAGCGCCCAGGCGGCGCCGCGCACGC‑CTCCGGCCACGGATGCCTGGAGCGCTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGc                                                                                                                          <  3:165861/149‑1 (MQ=255)                        cAGCGCCCAGGCGGCGCCGCGCGCGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGcc                                                                                                                         >  3:41409/1‑149 (MQ=255)                        cAGCGCCCAGGCGGCGCCGCGCACGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGcc                                                                                                                         >  2:89333/1‑149 (MQ=255)                           cgcCCAGGCGGCGCCGCGCACGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTc                                                                                                                      <  2:57012/149‑1 (MQ=255)                                        ccgcGCACGC‑CTCCGGCGACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATGGCAAGCCTTCGCGCGCACCGCGa                                                                                                         >  8:241350/1‑149 (MQ=255)                                         cgcgCACGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGAc                                                                                                        <  3:216195/149‑1 (MQ=255)                                           cgcACGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCa                                                                                                      <  2:263525/149‑1 (MQ=255)                                             cACGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAac                                                                                                    <  7:336304/149‑1 (MQ=255)                                              aCGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAaca                                                                                                   >  7:58884/1‑149 (MQ=255)                                                gC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACAcc                                                                                                 <  5:145898/149‑1 (MQ=255)                                                   cTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCCACGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACAccc                                                                                                <  1:89333/149‑1 (MQ=255)                                                           aCGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTg                                                                                       >  1:372839/1‑149 (MQ=255)                                                           aCGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTg                                                                                       <  2:294074/149‑1 (MQ=255)                                                                   atGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAg                                                                                <  5:152071/147‑1 (MQ=255)                                                                     ggAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCCCTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTgg                                                                             <  4:170806/149‑1 (MQ=255)                                                                      gAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTggg                                                                            <  4:41409/149‑1 (MQ=255)                                                                      gAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTg                                                                              <  5:307664/147‑1 (MQ=255)                                                                            ttttCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGTGCACAACTCGACCAacac                                                                                                  >  1:203144/1‑121 (MQ=255)                                                                            ttttCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGTGCACAACTCGACCAacac                                                                                                  <  2:203144/121‑1 (MQ=255)                                                                            ttttCGCCAACTGTTCGAGCTCGACCCCCACAACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTACGCGCACAACTATACCAACAACCGCCACGCTGCGTCGAGTGGGCCAcc                                                                       >  5:367389/1‑148 (MQ=255)                                                                                cGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGc                                                                                             <  6:175096/122‑1 (MQ=255)                                                                                cGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGc                                                                                             >  5:175096/1‑122 (MQ=255)                                                                                  ccAACTGTTTGAGCTCGACCCCCACTACGCGCACTTCGCCGACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGc                                                                 >  7:117802/1‑148 (MQ=255)                                                                                  ccAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGc                                                                 >  7:40547/1‑148 (MQ=255)                                                                                    aaCTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGc                                                                 >  4:128764/1‑146 (MQ=255)                                                                                    aaCTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGa                                                               <  3:128764/148‑1 (MQ=255)                                                                                    aaCTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAg                                                              <  8:40547/149‑1 (MQ=255)                                                                                     aCTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGt                                                             <  3:307869/149‑1 (MQ=255)                                                                                         ttCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCACCCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACCCCCAGAGCGAGTgg                                                           >  1:103542/1‑148 (MQ=255)                                                                                         ttCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTgg                                                           >  2:273672/1‑147 (MQ=255)                                                                                         ttCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGaa                                                         <  2:190726/149‑1 (MQ=255)                                                                                         ttCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTAGACAAACACCCGGCAAGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGaa                                                         >  6:273881/1‑149 (MQ=255)                                                                                             aGCGCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGATTGGGACACCCAGAGCGAGTGGAAGCAc                                                     <  7:241350/149‑1 (MQ=255)                                                                                              gCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACAccc                                                                      <  6:127060/131‑1 (MQ=255)                                                                                              gCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACAccc                                                                      >  5:127060/1‑131 (MQ=255)                                                                                              gCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACg                                                    <  8:58884/149‑1 (MQ=255)                                                                                                   aCCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGAGAATCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGcc                                               >  3:156326/1‑149 (MQ=255)                                                                                                     ccccACTACCCGCACTTCGACAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGCTCGCAAGCCTTCCCGCACAACTAGACCAACACCCGGCACCCTGCCTCGACTGGGACAACCACAGCGAGTGGAAGCACGaacgc                                               >  8:314513/1‑144 (MQ=255)                                                                                                      cccACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAg                                            >  3:108771/1‑149 (MQ=255)                                                                                                       ccACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAgg                                           >  6:122002/1‑149 (MQ=255)                                                                                                        cACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGt                                          <  2:103542/149‑1 (MQ=255)                                                                                                         aCTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTg                                         <  4:246883/149‑1 (MQ=255)                                                                                                          cTACGCGCACTTCGCCAACTTCCTCATCTCCTCGCA‑CACGCGCCC‑AGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGAGAGTGGAAGCACGAAGCCGAGGTGc                                        <  6:367389/149‑1 (MQ=255)                                                                                                              gcgcACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTg                                     >  3:211430/1‑148 (MQ=255)                                                                                                              gcgcACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGa                                    <  4:211430/149‑1 (MQ=255)                                                                                                                gcACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGTCCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAAt                                  >  1:121146/1‑149 (MQ=255)                                                                                                                gcACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAAt                                  <  7:122647/149‑1 (MQ=255)                                                                                                                gcACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAAt                                  >  6:310766/1‑149 (MQ=255)                                                                                                                gcACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAAt                                  >  1:17063/1‑149 (MQ=255)                                                                                                                 cACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATg                                 >  8:74030/1‑149 (MQ=255)                                                                                                                    ttCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGAc                                                                                                        <  1:287845/75‑1 (MQ=255)                                                                                                                    ttCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGAc                                                                                                        >  2:287845/1‑75 (MQ=255)                                                                                                                    ttCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTgg                                                                             >  3:345634/1‑102 (MQ=255)                                                                                                                    ttCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTgg                                                                             <  4:345634/102‑1 (MQ=255)                                                                                                                      cGCCGACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATgggc                              <  8:117802/147‑1 (MQ=255)                                                                                                                       gCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATgggcgg                            <  8:136676/148‑1 (MQ=255)                                                                                                                        ccAACTTCCTCATCGCCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGaa                                                         >  6:259736/1‑118 (MQ=255)                                                                                                                        ccAACTTCCTCATCGCCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGaa                                                         <  5:259736/118‑1 (MQ=255)                                                                                                                          aaCTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCg                        <  2:372839/149‑1 (MQ=255)                                                                                                                          aaCTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGAGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCg                        >  5:271150/1‑149 (MQ=255)                                                                                                                            cTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACAcc                                                                       >  6:121675/1‑100 (MQ=255)                                                                                                                            cTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACAcc                                                                       <  5:121675/100‑1 (MQ=255)                                                                                                                               ccTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGa                                             <  7:11089/123‑1 (MQ=255)                                                                                                                               ccTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGa                                             >  8:11089/1‑123 (MQ=255)                                                                                                                               ccTCATCACCTCGCA‑CCCGCGCAC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGATTGGAAGCACGAAGCCGAGGTGGGTGAATGGGCGGGCAGCTAt                    <  7:314513/148‑1 (MQ=255)                                                                                                                                cTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATc                   <  1:273672/148‑1 (MQ=255)                                                                                                                                cTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCt                  >  5:38354/1‑149 (MQ=255)                                                                                                                                 tcatcaCCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGCTCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCtt                 >  2:293073/1‑149 (MQ=255)                                                                                                                                 tcatcaCCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCtt                 >  5:17916/1‑149 (MQ=255)                                                                                                                                  catcaCCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGATCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGCCGGGCCGCTATCTTg                <  5:273881/149‑1 (MQ=255)                                                                                                                                    tcaCCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCTTGaa              <  5:310766/149‑1 (MQ=255)                                                                                                                                        cTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGc                       <  1:222759/136‑1 (MQ=255)                                                                                                                                        cTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGc                       >  2:222759/1‑136 (MQ=255)                                                                                                                                         tCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCTTGAAGTc           >  1:276943/1‑147 (MQ=255)                                                                                                                                           gCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCTTGAAGTCaa         <  6:17916/147‑1 (MQ=255)                                                                                                                                           gCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCTTGAAGTCaa         <  5:122002/147‑1 (MQ=255)                                                                                                                                           gCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCTTGAAGTCAAg        <  7:74030/148‑1 (MQ=255)                                                                                                                                              cttcgcgCAC‑GGGGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCTTGAAGTCaa         >  2:302321/4‑145 (MQ=255)                                                                                                                                              cttcgcgCAC‑GGGGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCTTGAAGTCaa         <  1:302321/142‑1 (MQ=255)                                                                                                                                                ccGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCTTGAAGTCAAGCCACg   <  4:108771/149‑1 (MQ=255)                                                                                                                                                 cgcgCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCTTGAAGTCAAGCCACGc  <  7:118973/149‑1 (MQ=255)                                                                                                                                                   cgcCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCACCACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCTTGAAGTCAAGCCACGc  >  5:60217/1‑147 (MQ=255)                                                                                                                                                      |                                                                                                                                                 CCCTTGGCCGGCCAACTGCTGGGCAGCGCCCAGGCGGCGCCGCGCACGC‑CTCCGGCCACGGATGCCTGGAGCGGTTTTCGCCAACTGTTCGAGCTCGACCCCCACTACGCGCACTTCGCCAACTTCCTCATCACCTCGCA‑CCCGCGCCC‑CGTGCGCGAAGCGATCGCAAGCCTTCGCGCACAACTCGACCAACACCCGGCACGCTGCGTCGAGTGGGACACCCAGAGCGAGTGGAAGCACGAAGCCGAGGTGCGTGAATGGGCGGGCCGCTATCTTGAAGTCAAGCCACGC  >  NC_002947/4761396‑4761686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑