Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 358,085 A→G 13.1% intergenic (+53/‑77) tdcG‑I → / → PP_5441 L‑serine dehydratase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947358,0850AG13.1% 110.8 / 8.0 49intergenic (+53/‑77)tdcG‑I/PP_5441L‑serine dehydratase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (19/22);  new base G (3/3);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.98e-01

ATCCGCACCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTT  >  NC_002947/357940‑358232
                                                                                                                                                 |                                                                                                                                                   
aTCCGCACCATGCGCGATACCGGCGCCGATATGCACTACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGTTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAAc                                                                                                                                                   <  5:152220/148‑1 (MQ=255)
aTCCGCACCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAAc                                                                                                                                                   <  4:362863/148‑1 (MQ=255)
 tCCGCACCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACtg                                                                                                                                                 >  3:78416/1‑149 (MQ=255)
   cGCACCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACtgtg                                                                                                                                               <  3:341486/149‑1 (MQ=255)
   cGCACCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGATATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACtgt                                                                                                                                                <  2:104831/148‑1 (MQ=255)
             gcgATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTg                                                                                                                                      >  4:347811/1‑148 (MQ=255)
             gcgATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTg                                                                                                                                      <  3:347811/148‑1 (MQ=255)
             gcgATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGt                                                                                                                                     <  8:43200/149‑1 (MQ=255)
               gATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGATTGCACAGTTCCTGTAg                                                                                                                                   <  7:257833/149‑1 (MQ=37)
                 tACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGa                                                                                                                                 >  4:237559/1‑149 (MQ=255)
                   ccGGCTCAGATATGCACGAAAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGc                                                                                                                               <  7:254157/149‑1 (MQ=25)
                   ccGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGc                                                                                                                               <  8:209533/149‑1 (MQ=37)
                    cGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCCCTCCTACAGGTACTGTGGAATCCCGGGAGGGGCg                                                                                                                              >  3:357340/1‑149 (MQ=255)
                    cGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCACTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCg                                                                                                                              >  5:181933/1‑149 (MQ=255)
                         ccGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCtt                                                                                                                         >  4:302261/1‑149 (MQ=255)
                         ccGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCtt                                                                                                                         <  3:237559/149‑1 (MQ=255)
                         ccGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCtt                                                                                                                         <  7:145563/149‑1 (MQ=255)
                         ccGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCtt                                                                                                                         <  8:100871/149‑1 (MQ=255)
                            atatGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGcc                                                                                                                      >  5:349935/1‑149 (MQ=255)
                               tGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCcg                                                                                                                    <  6:349935/148‑1 (MQ=255)
                                      cAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAgg                                                                                                            >  1:281785/1‑149 (MQ=255)
                                      cAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAAATGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAgg                                                                                                            >  4:86638/1‑149 (MQ=255)
                                       atgTACAACGCGACCGCGCGCGGCGGCCTGGCGGTCAGCGCTATCGTGTGCTGATTTTCTTAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGCAACTGTGCAATCCCTGTCGGCGGGGGCTTGCCCGCGAAGAGGc                                                                                                           <  4:78416/147‑1 (MQ=37)
                                              aaGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGATTGCACAGTTCCTGTAGGa                                                                                                                                 >  2:332396/1‑120 (MQ=25)
                                               agagACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAgg                                                                                                   <  3:302261/149‑1 (MQ=255)
                                               agagACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCGGCGTCAGGGACTGTGGGATGCCGGTTGGAGCGGGCTTGGCCGCGAAGAGGGCCGTGAgg                                                                                                   >  3:208494/1‑149 (MQ=255)
                                                      tCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCGACAGGAACTGTGCAATGCCTGTAGGCGCGGGCGTGGCCGCGCAGAGGCCCTTGCGGctgctg                                                                                             >  3:367743/1‑148 (MQ=37)
                                                         cgcgGCGGCCTGGCGGGCCGCGCTATCGAGTGCTTATTTTCTTAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCAACAGGAACTGTGCAATCCCTGTAGGAGGGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAgg                                                                                         <  2:281785/149‑1 (MQ=37)
                                                            ggcggcCTGGCGGTCAGCACTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGc                                                                                      <  6:181933/149‑1 (MQ=255)
                                                                                         cTGATTTTCTTAGCTGACGTTCTCGGCCTCTTGGCGGGCAAGCCGGGTCCTACAGGAACTGTGCAATCCCTGTAGTAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTg                                                         <  3:228346/149‑1 (MQ=38)
                                                                                           gATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCg                                                       >  7:224917/1‑149 (MQ=255)
                                                                                                       tGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGGAATCCCAGGAGGAGCGGGCTTGCCCGCGAAGCGGCCCGTGAGGCTGATGCCGGTGCACCACAACCGCCGATGCCTCTTTATGGTGCGGTGATGGGCGcc                                           >  8:208767/1‑149 (MQ=37)
                                                                                                       tGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGcc                                           >  5:179431/1‑149 (MQ=255)
                                                                                                                      ctTCGCGGGCAAGCCCCCTACAACAGGAACAGTGCAATACCAGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATTGTGCGGGGATGGGCGCCCCCCCAACCCAtagt                            >  8:144985/1‑149 (MQ=37)
                                                                                                                        tCGCGGGCAAGCCCGCTCCTACAGGCACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGAtcgtcg                          >  3:344867/1‑149 (MQ=255)
                                                                                                                         cgcgGCCACCCCCGCACCTACAGCAACTGTGCAATCCCTGTAGGAGGGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGAtcgtc                           <  7:144985/147‑1 (MQ=255)
                                                                                                                           cgGGCAAGCCCGCGCCTCCAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGAt                       <  3:86638/149‑1 (MQ=255)
                                                                                                                                aaGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTcgc                  >  6:149398/1‑149 (MQ=255)
                                                                                                                                  gCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCGGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGa                >  1:371811/1‑149 (MQ=255)
                                                                                                                                   cccGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGAt               >  2:284501/1‑149 (MQ=37)
                                                                                                                                   cccGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGAt               >  6:324449/1‑149 (MQ=255)
                                                                                                                                    ccGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTAAGGTGCGGTGAt                                                  <  2:127391/113‑1 (MQ=255)
                                                                                                                                    ccGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTAAGGTGCGGTGAt                                                  >  1:127391/1‑113 (MQ=255)
                                                                                                                                          cTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAgtgt         <  5:149398/148‑1 (MQ=255)
                                                                                                                                          cTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAgt           <  6:216019/146‑1 (MQ=255)
                                                                                                                                          cTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAgt           >  5:216019/1‑146 (MQ=255)
                                                                                                                                          cTACAGGAACTGGGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGtgtg        <  7:208767/149‑1 (MQ=255)
                                                                                                                                             cAGGACCTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGc     <  4:367743/149‑1 (MQ=255)
                                                                                                                                                gAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGtt  >  6:204336/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ATCCGCACCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTT  >  NC_002947/357940‑358232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑