Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,789,883 C→G 5.8% V1319V (GTG→GTC)  pvdI ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,789,8830CG 5.8% 223.8 / 3.5 69V1319V (GTG→GTC) pvdInon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (33/31);  new base G (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

TCATGGGCACGCCGATGTTGATCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCTTGCATCTGGCCTTCGCTCAGGCGCTCGCGTTGC  >  NC_002947/4789738‑4790030
                                                                                                                                                  |                                                                                                                                                   
tCATGGGCACGCCGATGTTGATCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACg                                                                                                                                                  >  7:147232/1‑149 (MQ=255)
  aTGGGCACGCCGATGTTGATCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTc                                                                                                                                                >  8:65129/1‑149 (MQ=255)
   tGGGCACGCCGATGTTGATCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTcc                                                                                                                                               <  1:30131/149‑1 (MQ=255)
     ggCACGCCGATGTTGATCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCa                                                                                                                                              <  5:50998/148‑1 (MQ=255)
          gCCGATGTTGATCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGC‑AGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTcc                                                                                                                                       >  5:109195/1‑149 (MQ=255)
           ccGATGTTGATCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTcc                                                                                                                                       >  3:308289/1‑149 (MQ=255)
           ccGATGTTGATCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTcc                                                                                                                                       <  3:105263/149‑1 (MQ=255)
            cGATGTTGATCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCg                                                                                                                                      >  7:137479/1‑149 (MQ=255)
               tGTTGATCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGccc                                                                                                                                   <  8:212278/149‑1 (MQ=255)
                gTTGATCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGCTAGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGccc                                                                                                                                   >  1:68406/1‑149 (MQ=255)
                   gATCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGa                                                                                                                                >  2:35624/1‑148 (MQ=255)
                    aTCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGATGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCCCGTGCCGACGTCCGCCCCGa                                                                                                                                >  8:254759/1‑147 (MQ=255)
                    aTCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGAt                                                                                                                               <  2:135647/148‑1 (MQ=255)
                        tctcGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCtt                                                                                                                          >  4:34211/1‑149 (MQ=255)
                         ctcGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCt                                                                                                                           >  7:196529/1‑147 (MQ=255)
                         ctcGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCt                                                                                                                           <  8:196529/147‑1 (MQ=255)
                            gTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGc                                                                                                                       <  5:48695/148‑1 (MQ=255)
                             ctgctgcTGGCGCGCTACAGCGGC‑AGGCCGAACGGGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAg                                                                                                                     >  2:54670/2‑149 (MQ=255)
                                      tAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGc                                                                                                              <  4:308289/147‑1 (MQ=255)
                                      tAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGGGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCg                                                                                                            <  4:262065/149‑1 (MQ=255)
                                        gcgcgcCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGTGCCGt                                                                                                           <  8:147232/148‑1 (MQ=255)
                                        gcgcgcCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGt                                                                                                           <  8:137479/148‑1 (MQ=255)
                                          gcgcCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGGACGTCTGGACGTGCGcct                                                                                                                                   <  1:69213/122‑2 (MQ=255)
                                          gcgcCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGGACGTCTGGACGTGCGcct                                                                                                                                   >  2:69213/1‑121 (MQ=255)
                                           cgcCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGc                                                                                                       >  1:282021/1‑149 (MQ=255)
                                             ccAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCgg                                                                                                     >  2:144768/1‑149 (MQ=255)
                                                   gc‑agGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCa                                                                                               <  7:65129/149‑1 (MQ=255)
                                                        gCCGAATGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGt                                                                                            >  4:263841/1‑148 (MQ=255)
                                                             aCGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTgc                                                                                      <  6:62568/149‑1 (MQ=255)
                                                               gAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTgcag                                                                                    <  1:35624/149‑1 (MQ=255)
                                                               gAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTgcag                                                                                    >  4:266577/1‑149 (MQ=255)
                                                                  gCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCAc                                                                                 >  3:77877/1‑149 (MQ=255)
                                                                  gCTAGGAAGACGTGGAACAGCGTGGCGTTACAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCAc                                                                                 >  1:28826/1‑149 (MQ=255)
                                                                      ggAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGc                                                                              <  8:15609/148‑1 (MQ=255)
                                                                         aGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGaaa                                                                          <  2:282021/149‑1 (MQ=255)
                                                                         aGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGaaa                                                                          <  1:121273/149‑1 (MQ=255)
                                                                         aGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGaaa                                                                          >  1:296111/1‑149 (MQ=255)
                                                                           aCGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAat                                                                         >  3:183071/1‑148 (MQ=255)
                                                                              tGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAatcatc                                                                     <  3:263841/149‑1 (MQ=255)
                                                                                gAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCtt                                                                   <  6:75695/149‑1 (MQ=255)
                                                                                 aaCAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTc                                                                  <  6:109195/149‑1 (MQ=255)
                                                                                    aGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCTGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAg                                                               <  2:170760/149‑1 (MQ=255)
                                                                                     gCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAgg                                                              <  3:34211/149‑1 (MQ=255)
                                                                                      cGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGt                                                             >  4:324172/1‑149 (MQ=255)
                                                                                      cGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGt                                                             >  5:294516/1‑149 (MQ=255)
                                                                                       gTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTg                                                            >  5:146728/1‑149 (MQ=255)
                                                                                        tGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTgc                                                           >  2:90969/1‑149 (MQ=255)
                                                                                               gCAGCGCACTGCCAGGCTCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGc                                                     <  1:90969/148‑1 (MQ=255)
                                                                                               gCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGc                                                     >  6:43467/1‑148 (MQ=255)
                                                                                                cAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGcc                                                    <  4:183071/148‑1 (MQ=255)
                                                                                                cAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGc                                                     >  3:270719/1‑147 (MQ=255)
                                                                                                        tGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGcc                                             >  2:108819/1‑147 (MQ=255)
                                                                                                          ccAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGc                                         >  3:97055/1‑149 (MQ=255)
                                                                                                          ccAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGc                                         >  6:313960/1‑149 (MQ=255)
                                                                                                           cAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCt                                        <  4:77877/149‑1 (MQ=255)
                                                                                                           cAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCt                                        <  2:296111/149‑1 (MQ=255)
                                                                                                              gcgcCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCGGCCAGTAGCCGAGCTggg                                     <  2:28826/149‑1 (MQ=255)
                                                                                                                gctgcAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCt                                   <  5:24402/146‑1 (MQ=255)
                                                                                                                gccgcAGGTCCGCCGTCACCTGCGCGGGGAGGCGCACGTCCAGACGTCCGCGCCTATAGCTTTGCAGCTCTGGCCGTGGGCGGTCGGc                                                                                                <  5:197527/88‑1 (MQ=255)
                                                                                                                gacgcAGGTCAGACGTCAACTGCGCGGGAAGGCGAAAGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGc                                                                                                >  6:197527/3‑88 (MQ=255)
                                                                                                                 ccgcAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCtt                                  <  1:144768/149‑1 (MQ=255)
                                                                                                                          cgccgTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCTTGCATCTgg                          <  2:68406/148‑1 (MQ=255)
                                                                                                                                 aaCTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCTTGCATCTGGCCTTCGCt                  >  4:345794/1‑149 (MQ=255)
                                                                                                                                    tGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCTTGCATCTGGCCTTCGCTCAg               >  7:165450/1‑149 (MQ=255)
                                                                                                                                         gggCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCTTGCATCTGGCCTTCGCTCAGGCGCt          >  3:100573/1‑149 (MQ=255)
                                                                                                                                             gggCGGACGTCTGGACGTGCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCTTGc                                >  7:328883/2‑123 (MQ=255)
                                                                                                                                             gggCGGACGTCTGGACGTGCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCTTGc                                <  8:328883/122‑1 (MQ=255)
                                                                                                                                             aGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCTTGCATCTGGCCTTCGCTCAGGCGCTcgcg      >  1:236175/1‑149 (MQ=255)
                                                                                                                                             aGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGAAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCTTGCATCTGGCCTTCGCTCAGGCGCTcgcg      >  1:109475/1‑149 (MQ=255)
                                                                                                                                               gcgcACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCTTGCATc                             >  5:78837/1‑124 (MQ=255)
                                                                                                                                                cgcACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCTTGCATCTGGCCTTCGCTCAGGCGCTCGCGTTg   <  5:43467/149‑1 (MQ=255)
                                                                                                                                                 gcACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCTTGCATCTGGCCTTCGCTCAGGCGCTCGCGTTGc  >  6:283910/1‑149 (MQ=255)
                                                                                                                                                 gcACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCTTGCATCTGGCCTTCGCTCAGGCGCTCGCGTTGc  <  1:54670/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TCATGGGCACGCCGATGTTGATCTTCTCGTTGCCGCTGTAGCGCGCCAGCAGC‑AGGCCGAACGAGGCTAGGAAGACGTGGAACAGCGTGGCGTTGCAGCGCACTGCCAGGCGCCGCAGGTCCGCCGTCAACTGCGCGGGCAGGCGCACGTCCAGACGTCCGCCCCGATAGCTTTGCAGCTCGGGCCGTGGGCGGTCGGCAGGTAACTGCAGCACTGCGAAATCATCTTCCAGGTGCGCCTGCCAGTAGCCGAGCTGGGCTTGCATCTGGCCTTCGCTCAGGCGCTCGCGTTGC  >  NC_002947/4789738‑4790030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑