Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,809,113 T→A 8.6% L223Q (CTG→CAG)  dsbD‑II → thiol/disulfide interchange protein 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,809,1130TA 8.6% 156.3 / 3.2 55L223Q (CTG→CAG) dsbD‑IIthiol/disulfide interchange protein 2
Reads supporting (aligned to +/- strand):  ref base T (22/28);  new base A (2/3);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCCTGCTCGTTGCCGATGCTGCCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTCTTCGAACTGCAACTGCCTGCCGCCCTGCGCGACCGCCTGGACCGCGCCGGGC  >  NC_002947/4808966‑4809259
                                                                                                                                                     |                                                                                                                                                  
cccccTGCTCGTTGCCGATGCTGCCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCctgctg                                                                                                                                                   >  1:196274/1‑149 (MQ=255)
 ccccTGCTCGTTGCCGATGCTGCCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTgg                                                                                                                                                  <  7:275962/149‑1 (MQ=255)
 ccccTGCTCGTTGCCGATGCTGCCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTgg                                                                                                                                                  >  5:254245/1‑149 (MQ=255)
 ccccTGCTCGTTGCCGATGCTGCCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCAATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTgg                                                                                                                                                  <  1:294693/149‑1 (MQ=255)
  cccTGCTCGTTGCCGATGCTGCCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTggg                                                                                                                                                 >  8:287583/1‑149 (MQ=255)
   ccTGCTCGTTGCCGATGCTGCCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGt                                                                                                                                                <  6:254245/149‑1 (MQ=255)
    cTGCTCGTTGCCGATGCTGCCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTg                                                                                                                                               >  1:139982/1‑149 (MQ=255)
               cGATGCTGCCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAg                                                                                                                                    >  2:192088/1‑149 (MQ=255)
                 aTGCTGCCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGc                                                                                                                                  <  4:282310/149‑1 (MQ=255)
                 aTGCTGCCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCAGCAGGCTGGCACCCAGCAggg                                                                                                                                  <  3:360831/149‑2 (MQ=255)
                    ctgcCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCt                                                                                                                                <  4:239092/148‑1 (MQ=255)
                     tgcCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTgg                                                                                                                              >  6:153128/1‑149 (MQ=255)
                     tgcCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTgg                                                                                                                              <  2:54227/149‑1 (MQ=255)
                       ccGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCt                                                                                                                            >  6:105691/1‑149 (MQ=255)
                       ccGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCt                                                                                                                            >  7:51635/1‑149 (MQ=255)
                        ggATCGTTGCGGGGGTGGTGGGGGGAAATGGCGCATGTGCCGGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTg                                                                                                                           <  2:376599/148‑1 (MQ=255)
                            ccTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTgcag                                                                                                                        >  1:296869/1‑148 (MQ=255)
                             cTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGCCAGGCCTgcagg                                                                                                                       <  4:25003/148‑2 (MQ=255)
                                    ggCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTgg                                                                                                               <  7:27584/149‑1 (MQ=255)
                                            cTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTggg                                                                                                       <  6:151631/149‑1 (MQ=255)
                                            cTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTggg                                                                                                       <  5:24672/149‑1 (MQ=255)
                                            cTGGGCAATGGCGCCAGGGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTggg                                                                                                       <  8:343269/149‑1 (MQ=255)
                                                   aTGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTg                                                                                                >  7:283562/1‑149 (MQ=255)
                                                   aTGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCCGCAGGCCTGGCTGCAGCAACCCTGGCTGCCCAGCAGccag                                                                                                <  2:196274/149‑3 (MQ=255)
                                                     ggCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGc                                                                                              >  4:48525/1‑149 (MQ=255)
                                                      gcgcCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCAGGCTGCCCAGCAGCCAgggt                                                                                             <  2:139982/149‑3 (MQ=255)
                                                          cAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGc                                                                                         >  3:186403/1‑149 (MQ=255)
                                                          cAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGc                                                                                         <  1:192088/149‑1 (MQ=255)
                                                            gTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTg                                                                                       >  7:114289/1‑149 (MQ=255)
                                                            agtgccGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTg                                                                                       >  5:162678/5‑149 (MQ=255)
                                                                ccGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGCGTGGGCTGGGGGT‑GGTTGCTGCCCTCATGGGTGCCAGCCTGCAGGCATGGCTCCAGCAACCATGGCTGCTGGGCAGCATGGCGGGGCTGTTc                                                                                    >  1:108415/1‑149 (MQ=255)
                                                                   gcgcGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGtgc                                                                                >  4:353290/1‑149 (MQ=255)
                                                                       ggctggctGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCAGGCTGGCACCCAGCAgg                                                                                                                                   >  2:34657/1‑94 (MQ=255)
                                                                       ggctggctGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GCTTGCTGCCCTGCAGGCTGGCACCCAGCAgg                                                                                                                                   <  1:34657/94‑1 (MQ=255)
                                                                            gctgctGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGccc                                                                       >  2:338705/1‑149 (MQ=255)
                                                                              tgctgGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTg                                                                     >  8:167951/1‑149 (MQ=255)
                                                                              tgctgGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTg                                                                     >  1:371716/1‑149 (MQ=255)
                                                                              tgctgGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCAGGCTGGCACCCAGCAgggc                                                                                                                                 <  5:93495/89‑3 (MQ=38)
                                                                              tgctgGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCAGGCTGGCACCCAGCAgggc                                                                                                                                 >  6:93495/1‑87 (MQ=38)
                                                                                ctgGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTg                                                                     <  3:353290/147‑1 (MQ=255)
                                                                                   gCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGa                                                                 <  4:340471/148‑1 (MQ=255)
                                                                                        ggTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCg                                                           <  2:296869/149‑1 (MQ=255)
                                                                                         gTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCgg                                                          <  7:167951/149‑1 (MQ=255)
                                                                                          tCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGc                                                         <  8:283562/149‑1 (MQ=255)
                                                                                          tCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGc                                                         <  8:114289/149‑1 (MQ=255)
                                                                                              tgtgCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTc                                                      <  6:162678/148‑1 (MQ=255)
                                                                                              tgtgCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTCt                                                     <  2:108415/149‑1 (MQ=255)
                                                                                                tgCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCGGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTCtt                                                    <  6:298346/148‑1 (MQ=255)
                                                                                                       cATGGCCCTGGTGTACGC‑CGGGCTGGGGGTAGGTTGCTGCCCTGCTGGGTGCCCGCCTGCAGGCCTGGCTGCAGCACCCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTCTTCGaactg                                             >  1:34698/1‑149 (MQ=255)
                                                                                                             ccTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTCTTCGaactgcaactgc                                      <  6:12903/149‑1 (MQ=255)
                                                                                                                 gtgtACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGccc                                                                       <  3:14848/112‑1 (MQ=255)
                                                                                                                 gtgtACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGccc                                                                       >  4:14848/1‑112 (MQ=255)
                                                                                                                               tGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTCTTCGAACTGCAACTGCCTGCCGCCCTGCGCGAc                     >  8:129114/1‑149 (MQ=255)
                                                                                                                                        gtTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTCTTCGAACTGCAACTGCCTGCCGCCCTGCGCGACCGCCTGGa             <  3:335411/149‑1 (MQ=255)
                                                                                                                                             tgcCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTCTTCGAACTGCAACTGCCTGCCGCCCTGCGCGACCGCCTGGACcgcg        >  3:347069/1‑149 (MQ=255)
                                                                                                                                               cccTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTCTTCGAACTGCAACTGCCTGCCGCCCTGCGCGACCGCCTGGACCGCGcc      <  3:48525/149‑1 (MQ=255)
                                                                                                                                                 ctgctgGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTCTTCGaactgcaactgc                                      <  7:307338/115‑1 (MQ=255)
                                                                                                                                                 ctgctgGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTCTTCGaactgcaactgc                                      >  8:307338/1‑115 (MQ=255)
                                                                                                                                                  tgctgGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTCTTCGAACTGCAACTGCCTGCCGCCCTGCGCGACCGCCTGGACCGCGCCggg   >  2:165491/1‑149 (MQ=255)
                                                                                                                                                     tgGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTCTTCGAACTGCAACTGCCTGCCGCCCTGCGCGACCGCCTGGACCGCGCCGGGc  >  1:364667/1‑147 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCCCCTGCTCGTTGCCGATGCTGCCGATCCTTGCCGGGCTGGTGCTGGGCAATGGCGCCAGTGCCCGGCGCGGCTGGCTGCTGGCCGGGGTCTATGTGCTGAGCATGGCCCTGGTGTACGC‑CGGGCTGGGGGT‑GGTTGCTGCCCTGCTGGGTGCCAGCCTGCAGGCCTGGCTGCAGCAACCCTGGCTGCTGGGCAGCCTGGCGGGGCTGTTCGTGCTGCTGGCCCTGCCGATGTTCGGCGTCTTCGAACTGCAACTGCCTGCCGCCCTGCGCGACCGCCTGGACCGCGCCGGGC  >  NC_002947/4808966‑4809259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑