Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,927,502 T→C 9.5% H127R (CAC→CGC) ‡ cheBA ← chemotaxis response regulator protein‑glutamate methylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,927,5020TC 9.5% 170.1 / 8.0 63H127R (CAC→CGC) ‡cheBAchemotaxis response regulator protein‑glutamate methylesterase
Reads supporting (aligned to +/- strand):  ref base T (26/31);  new base C (3/3);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

CGAGGCCGCCGGCGTCGGGGCCGCTGCACGGGCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGCGGGCGCCTTCATGGGTCAGCGACGAGAACATC  >  NC_002947/4927354‑4927645
                                                                                                                                                    |                                                                                                                                               
cGAGGCCGCCGGCGTCGGGGCCGCTGCACGGGCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGgtgt                                                                                                                                                 <  2:162645/149‑1 (MQ=255)
cGAGGCCGCCGGCGTCGGGGCCGCTGCACGGGCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGgtgt                                                                                                                                                 <  1:61481/149‑1 (MQ=255)
    gccgccGGCGTCGGGGCCGCTGCACGGGCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCAc                                                                                                                                             >  3:374154/1‑149 (MQ=255)
         cgGCGTCGGGGCCGCTGCACGGGCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTtct                                                                                                                                        >  5:80754/1‑149 (MQ=255)
          ggCGTCGGGGCCGCTGCACGGGCAGGCGCTGCCGGGCACGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTtctc                                                                                                                                       <  7:22607/149‑1 (MQ=255)
                 gggCCGCGGCACGGGCAGGCGAGGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGTCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCTTAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACGTTCTCGCACAGc                                                                                                                                <  8:275797/149‑1 (MQ=255)
                   gCCGCTGCACGGGCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCaa                                                                                                                              >  4:151338/1‑149 (MQ=255)
                   gCCGCTGCACGGGCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCaa                                                                                                                              >  6:84223/1‑149 (MQ=255)
                     cGCTGCACGGGCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGTGCGAGAAGGTGCACACCATTTCGCGCAGCAAcc                                                                                                                            <  6:59346/149‑2 (MQ=38)
                     cGCTGCACGGGCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACt                                                                                                                            >  2:185229/1‑149 (MQ=255)
                          cACGGGCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTg                                                                                                                       <  5:144578/149‑1 (MQ=255)
                          cACGGGCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTg                                                                                                                       <  6:112989/149‑1 (MQ=255)
                          cACGGGCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGGTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTg                                                                                                                       <  5:179765/149‑1 (MQ=255)
                            cGGGCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGAc                                                                                                                     <  3:151338/149‑1 (MQ=255)
                               gCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCtt                                                                                                                  >  3:233320/1‑149 (MQ=255)
                                 aGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGt                                                                                                                <  1:296041/149‑1 (MQ=255)
                                 aGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGt                                                                                                                <  4:115380/149‑1 (MQ=255)
                                  ggCGCAGACGCGCCCGGCTGCGCCTGGCTGGTAAAGGTGCTGGTCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGt                                                                                                                >  4:233037/1‑148 (MQ=255)
                                   gcgcTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCa                                                                                                              <  4:374154/149‑1 (MQ=255)
                                      cTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAggg                                                                                                           <  6:80754/149‑1 (MQ=255)
                                      cTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAggg                                                                                                           <  1:191125/149‑1 (MQ=255)
                                       tGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTtctc                                                                                                                                       <  8:144936/120‑1 (MQ=255)
                                       tGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTtctc                                                                                                                                       >  7:144936/1‑120 (MQ=255)
                                            ggCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTgcgc                                                                                                     <  4:370025/149‑1 (MQ=255)
                                                 gTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGAt                                                                                                >  6:6687/1‑149 (MQ=255)
                                                      cgcCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTc                                                                                             <  1:177343/147‑1 (MQ=255)
                                                            gctggTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGt                                                                                     >  5:351087/1‑149 (MQ=255)
                                                                  aaaGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCg                                                                               >  8:49225/1‑149 (MQ=255)
                                                                     ggTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAAc                                                                                                                             <  1:21126/100‑1 (MQ=255)
                                                                     ggTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAAc                                                                                                                             >  2:21126/1‑100 (MQ=255)
                                                                       tGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAgg                                                                          <  5:6687/149‑1 (MQ=255)
                                                                         cTGGCCGGCGCTGGCGCGGCGACCGTCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACNTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGt                                                                         <  5:100231/148‑1 (MQ=255)
                                                                         cTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAgg                                                                          >  4:150677/1‑147 (MQ=255)
                                                                            gCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTc                                                                     <  4:129821/149‑1 (MQ=255)
                                                                                    tGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCga                                                                                                   >  6:356614/1‑111 (MQ=255)
                                                                                    tGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCga                                                                                                   <  5:356614/111‑1 (MQ=255)
                                                                                      gcgcGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGcc                                                            <  1:185229/148‑1 (MQ=255)
                                                                                      gcgcGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCg                                                           <  5:84223/149‑1 (MQ=255)
                                                                                        gcggcgACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTgc                                                                                                       >  4:133294/1‑103 (MQ=255)
                                                                                           gcgACCGGCTCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATTGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTTCGCGAGATGTCATCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATc                                                      <  8:330642/149‑1 (MQ=255)
                                                                                                cGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATCTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAgcg                                                 >  2:235115/1‑149 (MQ=255)
                                                                                                          cTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAggg                                        <  4:233320/148‑1 (MQ=255)
                                                                                                            ggCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTACTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGa                                     >  4:203848/1‑149 (MQ=255)
                                                                                                             gCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGAc                                    >  4:236483/1‑149 (MQ=255)
                                                                                                             gCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGAc                                    >  3:335847/1‑149 (MQ=255)
                                                                                                               aTAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAgc                                                  >  2:310222/1‑133 (MQ=255)
                                                                                                               aTAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAgc                                                  <  1:310222/133‑1 (MQ=255)
                                                                                                                   cTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGCGGgc                              <  3:157288/149‑1 (MQ=255)
                                                                                                                         aTCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGCGGGCGCCTTc                        <  3:236483/149‑1 (MQ=255)
                                                                                                                         aTCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGCGGGCGCCTTc                        <  6:351087/149‑1 (MQ=255)
                                                                                                                            cggcggTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAATTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGCGGGCGCCTTCAt                      <  3:233037/148‑1 (MQ=255)
                                                                                                                              gcggTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGCGGGCGCCTTCATggg                   >  7:236022/1‑149 (MQ=255)
                                                                                                                                 gTTGCTGTGCGAGAAGGTGCACACCATTTCGCGCAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGCGGGCGCCTTCATGGGt                  >  2:94306/1‑147 (MQ=38)
                                                                                                                                 gTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGCGGGCGCCTTCATGGGt                  >  4:332626/1‑147 (MQ=255)
                                                                                                                                    gctgcGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGCGGGCGCCTTCATGGGTCAGc              >  7:101843/1‑148 (MQ=255)
                                                                                                                                    gctgcGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGAGGGCGCCTTCATGGGTCAGcg             >  4:32011/1‑149 (MQ=255)
                                                                                                                                      tgtgcgAGAAGGTGCACACCATTTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGttttt                                                                                 <  2:19543/76‑1 (MQ=21)
                                                                                                                                      tgtgcgAGAAGGTGCACACCATTTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGttttt                                                                                 >  1:25393/4‑79 (MQ=21)
                                                                                                                                      tgtgcgAGAAGGTGCACACCATTTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGttttt                                                                                 <  2:25393/76‑1 (MQ=21)
                                                                                                                                      tgtgcgAGAAGGTGCACACCATTTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGttttt                                                                                 >  1:19543/4‑79 (MQ=21)
                                                                                                                                      tgcGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGAGCGTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATGCAGCGCGTCGAGGGTGTCGAGGGCGACTTCATGGGTCAGcgac           >  6:282908/1‑149 (MQ=255)
                                                                                                                                          cgAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGCGGGCGCCTTCATGGGTCAGCGACgaga       <  4:293927/149‑1 (MQ=255)
                                                                                                                                               tGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGCGGGCGCCTTCATGGGTCAGCGACGAGAACAt   >  2:225284/1‑148 (MQ=255)
                                                                                                                                               tGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGCGGGCGCCTTCATGGGTCAGCGACGAGAACATc  <  8:236022/149‑1 (MQ=255)
                                                                                                                                               tGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGCGGGCGCCTTCATGGGTCAGCGACGAGAACATc  <  3:332626/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CGAGGCCGCCGGCGTCGGGGCCGCTGCACGGGCAGGCGCTGCCGGGCGCGTCTGCGCCTGGCTGGTAAAGGTGCTGGCCGGCGCTGGCGCGGCGACCGGCGCCGTTCTGGCATAGCTGCCGATCCGGCGGTTGCTGCGCGAAATGGTGTGCACCTTCTCGCACAGCAACTGCTTGACCTTGTCAGGGTTGCGCGAGATGTCCTCGAAGTTTTTCGGCAGGTAGTCCACTGCGCCGGCATCCAGCGCGTCGAGGGTGACGCGGGCGCCTTCATGGGTCAGCGACGAGAACATC  >  NC_002947/4927354‑4927645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑