Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,003,016 A→T 6.1% R267R (CGA→CGT)  PP_4409 → site‑specific recombinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,003,0160AT 6.1% 191.8 / 2.4 65R267R (CGA→CGT) PP_4409site‑specific recombinase
Reads supporting (aligned to +/- strand):  ref base A (28/33);  new base T (2/2);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

ACTGGACAAGGTTACCGACCTCATCACCAAGGCAAGCTGCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCTGGAACCTTATGCCAGCCTCTTTTTTC  >  NC_002947/5002878‑5003164
                                                                                                                                          |                                                                                                                                                    
aCTGGACAAGGTTACCGACCTCATCACCAAGGCAAGCTGCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTc                                                                                                                                            <  5:157009/149‑1 (MQ=255)
    gACAAGGTTACCTACCTCATCACCAAGGCAAGCTGCTATCGAAACTAATGTTTCTACGCACTTATTGCAGCAACAAATTCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGt                                                                                                                                        <  5:273433/149‑1 (MQ=255)
    gACAAGGTTACCGACCTCATCACCAAGGCAAGCTGCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAAAACTGTGGCGCGACATAAACTTCTCGTGATGTGAAGtttatgtcgc                                                                                                                                        <  1:270611/149‑8 (MQ=255)
     aCAAGGTTACCGACCTCATCACCAAGGCAAGCTGCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGt                                                                                                                                        >  3:346796/1‑148 (MQ=255)
     aCAAGGTTACCGACCTCATCACCAAGGCAAGCTGCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGAGCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTc                                                                                                                                       <  7:342501/149‑1 (MQ=255)
             aCCGACCTCATCACCAAGGCAAGCTGCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCTCGTGATGTGAAGtttat                                                                                                                                             <  1:116335/135‑3 (MQ=255)
             aCCGACCTCATCACCAAGGCAAGCTGCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCTCGTGATGTGAAGtttat                                                                                                                                             >  2:116335/1‑133 (MQ=255)
               cGACCTCATCACCAAGGCAAGCTGCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTg                                                                                                                                         >  6:166379/1‑137 (MQ=255)
               cGACCTCATCACCAAGGCAAGCTGCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTg                                                                                                                                         <  5:166379/137‑1 (MQ=255)
                      atcaCCAAGGCAAGCTTCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCTCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGAc                                                                                                                       <  1:366723/148‑1 (MQ=255)
                         aCCAAGGCAAGCTGCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACTAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCaa                                                                                                                   >  3:280742/1‑149 (MQ=255)
                          ccAAGGCAAGCTGCTATCGAACCTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGGGCAATTCTGAAGCTGATCAAAAACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTGTTGTCAACCCCCAAACCCGACTAAAt                                                                                                                  >  3:306810/1‑149 (MQ=255)
                          ccAAGGCAAGCTGCTATCGAAACTCATGATTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACACGACTCAAt                                                                                                                  >  7:150916/1‑149 (MQ=255)
                              gccAAGCTGCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTg                                                                                                              <  5:333506/147‑1 (MQ=255)
                                 aaGCTGCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTgg                                                                                                             >  1:147062/1‑147 (MQ=255)
                                 aaGCTGCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTgg                                                                                                             <  2:147062/147‑1 (MQ=255)
                                      gctATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCt                                                                                                      >  1:12773/1‑149 (MQ=255)
                                       ctATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGc                                                                                                       <  4:346796/147‑1 (MQ=255)
                                         aTCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTAt                                                                                                   >  2:131551/1‑149 (MQ=255)
                                          tCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATc                                                                                                  >  2:180396/1‑149 (MQ=255)
                                            gAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTAATGCTTATCGt                                                                                                <  3:170233/149‑1 (MQ=255)
                                             aaaCTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTg                                                                                               >  1:322684/1‑149 (MQ=255)
                                             aaaCTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTg                                                                                               <  7:345613/149‑1 (MQ=255)
                                             aaaCTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTg                                                                                               >  7:300618/1‑149 (MQ=255)
                                              aaCTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTgg                                                                                              >  5:64804/1‑149 (MQ=255)
                                              aaCTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTgg                                                                                              <  1:281456/149‑1 (MQ=255)
                                                   aTGATTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGa                                                                                         <  8:150916/149‑1 (MQ=255)
                                                            cGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGaaa                                                                                   <  5:154790/146‑1 (MQ=255)
                                                            cGCACTTACTCCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATCCTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATc                                                                                 <  4:306810/148‑1 (MQ=255)
                                                               aCTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTg                                                                                                              <  6:149669/116‑1 (MQ=255)
                                                               aCTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTg                                                                                                              >  5:149669/1‑116 (MQ=255)
                                                               aCTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTg                                                                                               <  6:176178/131‑1 (MQ=255)
                                                               aCTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTg                                                                                               >  5:176178/1‑131 (MQ=255)
                                                               aCTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGt                                                                                       <  6:157503/139‑1 (MQ=255)
                                                               aCTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGt                                                                                       >  5:157503/1‑139 (MQ=255)
                                                               aCTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGc                                                                             >  7:132272/1‑149 (MQ=255)
                                                                   aTTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGaa                                                                          >  4:175899/1‑148 (MQ=255)
                                                                    ttGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGaaac                                                                        <  8:132272/149‑1 (MQ=255)
                                                                    ttGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGaaac                                                                        <  2:322684/149‑1 (MQ=255)
                                                                    ttGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGGGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGaaa                                                                         <  2:50663/148‑1 (MQ=255)
                                                                          caacaaATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTc                                                                  <  1:131551/149‑1 (MQ=255)
                                                                             caaATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGaa                                                               <  4:280742/149‑1 (MQ=255)
                                                                                   cgcgcAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATgg                                                            >  2:361450/1‑146 (MQ=255)
                                                                                   cgcgcAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGaa                                                          <  1:361450/148‑1 (MQ=255)
                                                                                    gcgcAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAAACCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGACACCAACTCGAATGGCAgg                                                        >  4:295193/1‑149 (MQ=255)
                                                                                               aaGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTgg                                                                                                             >  8:16761/1‑85 (MQ=255)
                                                                                               aaGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTgg                                                                                                             <  7:16761/85‑1 (MQ=255)
                                                                                                     aTCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGa                                                                               >  1:295666/1‑109 (MQ=255)
                                                                                                     aTCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGa                                                                               <  2:295666/109‑1 (MQ=255)
                                                                                                      tCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTa                                      >  7:323416/1‑149 (MQ=255)
                                                                                                         aaTACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCa                                   >  1:170842/1‑149 (MQ=255)
                                                                                                                tgGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCt                            >  5:127599/1‑149 (MQ=255)
                                                                                                                  gcgcGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCTg                           <  6:64804/148‑1 (MQ=255)
                                                                                                                    gcgACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCTGGa                         <  2:6029/148‑1 (MQ=255)
                                                                                                                    gcgACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCTGGa                         >  1:6029/1‑148 (MQ=255)
                                                                                                                      aagaaaaaCTTCTCGTGATGTGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCTGGAAcc                      >  3:101736/6‑149 (MQ=255)
                                                                                                                       aCATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTa                                      <  7:329310/132‑1 (MQ=255)
                                                                                                                        cATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCTGGAACCtt                    <  6:127599/149‑1 (MQ=255)
                                                                                                                         aTAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAAAAAACTAGAATGGAAGGGTCGGGGCACACACCTTACCATTATGAGGGAACATTa                   >  5:343172/1‑149 (MQ=255)
                                                                                                                          tAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCTGGAACCTTAt                  >  2:117528/1‑149 (MQ=255)
                                                                                                                           aaaCTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCTGGAACCTTATg                 <  8:300618/149‑1 (MQ=255)
                                                                                                                            aaCTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCTGGAACCTTATGc                <  3:175899/149‑1 (MQ=255)
                                                                                                                                    aTCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCTGGAACCTTATGCCAGCctct        >  2:354616/1‑149 (MQ=255)
                                                                                                                                     tCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCTGGAACCTTATGCCAGCCTCtt       <  1:354616/149‑1 (MQ=255)
                                                                                                                                        cGAGAAGTTTTTCTTGTCAACCCCCATACCCTCCTCACTCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCTGGAACCTTATGCCAGCCTCttttt    <  3:295193/149‑1 (MQ=255)
                                                                                                                                          agaAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCTGGAACCTTATGCCAGCCTCTTTTTTc  <  5:323208/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                    
ACTGGACAAGGTTACCGACCTCATCACCAAGGCAAGCTGCTATCGAAACTCATGTTTCTACGCACTTATTGCAGCAACAAATGCGCGCAATTCTGAAGCTGATCAAATACTGTGGCGCGACATAAACTTCACATCACGAGAAGTTTTTCTTGTCAACCCCCATACCCGACTCAATCCTGGTGATGCTTATCGTGGGCTGAGTGAAATCGAGCGAAACAAACTCGAATGGAAGGGTCGGGGCACCCCACTTACCATTATGCTGGAACCTTATGCCAGCCTCTTTTTTC  >  NC_002947/5002878‑5003164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑