Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,064,612	C→A	8.3%	G129C (GGT→TGT) ‡	PP_4461 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,064,612	0	C	A	8.3%	293.4 / 13.9	96	G129C (GGT→TGT) ‡	PP_4461	MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (45/43);  new base A (4/4);  total (49/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01

GCAGGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCT  >  NC_002947/5064464‑5064759                                                                                                                                                      |                                                                                                                                                    gcagGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTTCGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCAcc                                                                                                                                                     >  5:286989/1‑149 (MQ=255) gcagGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCAcc                                                                                                                                                     >  3:225716/1‑149 (MQ=255)  cagGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCt                                                                                                                                                    >  2:272108/1‑149 (MQ=255)  cagGAATGAGCAAGAGCGCCGAGGCTACTACCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCt                                                                                                                                                    >  4:149708/1‑149 (MQ=255)    caaaTGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgc                                                                                                                                                  <  3:284854/147‑1 (MQ=255)     gAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcg                                                                                                                                                 >  1:191158/1‑149 (MQ=255)     gAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcg                                                                                                                                                 >  8:343322/1‑149 (MQ=255)      aaTGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcgg                                                                                                                                                <  1:272108/149‑1 (MQ=255)       aTGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcggc                                                                                                                                               <  7:189656/149‑1 (MQ=255)         gAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACCACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATACGCCACCTgcggcg                                                                                                                                              >  7:246767/1‑148 (MQ=255)          aGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTACACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGaa                                                                                                                                            <  2:367397/149‑1 (MQ=255)          aGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTACACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGaa                                                                                                                                            >  1:367397/1‑149 (MQ=255)           acAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAAt                                                                                                                                           >  6:126604/2‑149 (MQ=255)             aaGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtgg                                                                                                                                         <  5:119381/149‑3 (MQ=255)               gagCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCtt                                                                                                                                       >  2:53843/1‑149 (MQ=255)                agCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                      <  5:31840/149‑1 (MQ=255)                agCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                      <  3:228582/149‑1 (MQ=255)                  cgcCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                      >  5:71471/1‑147 (MQ=255)                  cgcCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                    <  7:297842/149‑1 (MQ=255)                       aGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTTCGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACAt                                                                                                                               <  6:286989/149‑1 (MQ=255)                         gCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACAtc                                                                                                                              >  6:171909/1‑148 (MQ=255)                             ctaGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCa                                                                                                                           <  4:225716/147‑1 (MQ=255)                                gCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtggg                                                                                                                                        >  6:369139/1‑128 (MQ=255)                                gCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtggg                                                                                                                                        <  5:369139/131‑4 (MQ=255)                                gCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACAt                                                                                                                       >  5:119173/1‑148 (MQ=255)                                gCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACAt                                                                                                                       >  2:123240/1‑148 (MQ=255)                                  gAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGAc                                                                                                                    <  4:205478/149‑1 (MQ=255)                                  gAGGCCAGAAAAGCTCCCCCAGAGGAGCTCACGCTATGGAAACTTGCTAAGTATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCGTTGGAGACATCTCAACATGAc                                                                                                                    <  8:246767/149‑1 (MQ=255)                                    ggCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGAc                                                                                                                    >  1:337925/1‑147 (MQ=255)                                    ggCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTa                                                                                                                  <  5:350185/149‑1 (MQ=255)                                     gCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAg                                                                                                                 >  8:164264/1‑149 (MQ=255)                                      caaGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAg                                                                                                                 >  4:237587/3‑148 (MQ=255)                                       cAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAg                                                                                                                 >  3:68057/1‑147 (MQ=255)                                        aGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtg                                                                                                                                          <  4:216250/121‑2 (MQ=255)                                        aGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtg                                                                                                                                          >  3:216250/1‑120 (MQ=255)                                        aGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAg                                                                                                                 >  2:314735/1‑146 (MQ=255)                                        aGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAg                                                                                                              <  1:314735/149‑1 (MQ=255)                                         gCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAg                                                                                                              >  2:133325/1‑148 (MQ=255)                                              gcgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                      >  7:294510/1‑119 (MQ=255)                                              gcgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                      <  8:294510/119‑1 (MQ=255)                                               cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                       <  4:68057/149‑1 (MQ=255)                                               cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                       <  1:123240/149‑1 (MQ=255)                                               cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                       <  6:71471/149‑1 (MQ=255)                                               cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                       <  7:164264/149‑1 (MQ=255)                                               cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                       <  2:118014/149‑1 (MQ=255)                                                   cccAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCAc                                                                                                   <  7:142189/149‑1 (MQ=255)                                                   cccAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCAc                                                                                                   <  6:119173/149‑1 (MQ=255)                                                                   ctctGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGtctc                                                                                   <  5:126604/149‑1 (MQ=255)                                                                    tctGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGTGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATa                                                                                        <  2:70396/143‑1 (MQ=255)                                                                    tctGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGGGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATa                                                                                        >  1:70396/1‑143 (MQ=255)                                                                       ggAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTAt                                                                               >  8:33804/1‑149 (MQ=255)                                                                          aaCTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCACCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGtt                                                                            >  6:222163/1‑149 (MQ=255)                                                                             ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcgg                                                                                                                                                >  4:120184/1‑78 (MQ=255)                                                                             ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcgg                                                                                                                                                <  3:120184/78‑1 (MQ=255)                                                                             ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGa                                                                                                                     <  3:333263/105‑1 (MQ=255)                                                                             ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGa                                                                                                                     >  4:333263/1‑105 (MQ=255)                                                                             ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGAGAATCCTTGGAGACATATCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTgg                                                                          >  8:109964/1‑148 (MQ=255)                                                                              tCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGaa                                                                        >  8:276545/1‑149 (MQ=255)                                                                               ccAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGaa                                                                        >  7:282789/1‑148 (MQ=255)                                                                                cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGTCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGtt                                                                            <  8:171549/143‑1 (MQ=255)                                                                                cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                    >  2:64114/1‑87 (MQ=255)                                                                                cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                    <  1:64114/87‑1 (MQ=255)                                                                                cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTAGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAAt                                                                       >  1:345278/1‑148 (MQ=255)                                                                                cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGACACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGtt                                                                            >  7:171549/1‑143 (MQ=255)                                                                                 aaaGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAAt                                                                       >  5:93090/1‑147 (MQ=255)                                                                                    gAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGac                                                                  <  3:149708/149‑1 (MQ=255)                                                                                    gAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGac                                                                  <  7:343322/149‑1 (MQ=255)                                                                                     aaTCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGacc                                                                 <  1:53843/149‑1 (MQ=255)                                                                                     aaTCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGac                                                                  >  6:182385/1‑148 (MQ=255)                                                                                       tCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaa                                                               <  5:171909/149‑1 (MQ=255)                                                                                          cTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCGATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTg                                                                           <  2:343882/134‑1 (MQ=255)                                                                                          cTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTg                                                                           >  1:343882/1‑134 (MQ=255)                                                                                          cTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaatac                                                            <  2:337925/149‑1 (MQ=255)                                                                                           ttACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaatac                                                            >  5:289946/1‑148 (MQ=255)                                                                                                aCCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACcaataccaatac                                                      >  7:119966/1‑149 (MQ=255)                                                                                                   tGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGc                                                   >  5:124155/1‑149 (MQ=255)                                                                                                    gCAGGAGACCATTCCACGATATAAGAGTGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                       >  1:3529/1‑97 (MQ=255)                                                                                                    gCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                       <  2:3529/96‑1 (MQ=255)                                                                                                        gagaCCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGAtt                                              >  4:343534/1‑149 (MQ=255)                                                                                                         agaCCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTg                                             <  1:133325/149‑1 (MQ=255)                                                                                                             cATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCAt                                         >  1:75851/1‑149 (MQ=255)                                                                                                             cATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGAAATCAGACCAATACCAATACTGCCGATTGCCAt                                         <  2:191158/149‑1 (MQ=255)                                                                                                                  cACGATATAAGA‑TGCAGAGGTGCACCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCaaa                                    <  5:222163/149‑1 (MQ=255)                                                                                                                       tataAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATc                               <  6:93090/149‑1 (MQ=255)                                                                                                                         taAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCATAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGt                             <  3:237587/149‑1 (MQ=255)                                                                                                                               tGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGc                                                   >  7:11043/1‑122 (MQ=255)                                                                                                                               tGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGc                                                   <  8:11043/122‑1 (MQ=255)                                                                                                                                    agGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATc                   <  6:124155/149‑1 (MQ=255)                                                                                                                                       tGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATg                                        <  2:321287/125‑1 (MQ=255)                                                                                                                                       tGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATg                                        >  1:321287/1‑125 (MQ=255)                                                                                                                                        caaggaTTCGCCGCAGGTGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCgg               >  2:17825/6‑149 (MQ=255)                                                                                                                                         ccccATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCggg              >  5:341716/1‑149 (MQ=255)                                                                                                                                         ccacATTAGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCggg              >  4:273925/4‑149 (MQ=255)                                                                                                                                           ccATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCt            >  5:254777/1‑149 (MQ=255)                                                                                                                                           ccATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTAGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCt            >  1:294783/1‑149 (MQ=255)                                                                                                                                             aTTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTg          >  2:214165/1‑149 (MQ=255)                                                                                                                                              ttCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTgc         <  3:343534/149‑1 (MQ=255)                                                                                                                                                 gCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTgcgc       <  5:182385/148‑1 (MQ=255)                                                                                                                                                     ccTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCt  <  2:294783/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    GCAGGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCT  >  NC_002947/5064464‑5064759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑