Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,064,613	T→G	8.3%	A128A (GCA→GCC) ‡	PP_4461 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,064,613	0	T	G	8.3%	276.4 / 13.4	95	A128A (GCA→GCC) ‡	PP_4461	MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (43/44);  new base G (4/4);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

CAGGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCT  >  NC_002947/5064465‑5064759                                                                                                                                                      |                                                                                                                                                   cagGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCt                                                                                                                                                    >  2:272108/1‑149 (MQ=255) cagGAATGAGCAAGAGCGCCGAGGCTACTACCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCt                                                                                                                                                    >  4:149708/1‑149 (MQ=255)   caaaTGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgc                                                                                                                                                  <  3:284854/147‑1 (MQ=255)    gAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcg                                                                                                                                                 >  1:191158/1‑149 (MQ=255)    gAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcg                                                                                                                                                 >  8:343322/1‑149 (MQ=255)     aaTGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcgg                                                                                                                                                <  1:272108/149‑1 (MQ=255)      aTGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcggc                                                                                                                                               <  7:189656/149‑1 (MQ=255)        gAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACCACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATACGCCACCTgcggcg                                                                                                                                              >  7:246767/1‑148 (MQ=255)         aGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTACACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGaa                                                                                                                                            >  1:367397/1‑149 (MQ=255)         aGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTACACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGaa                                                                                                                                            <  2:367397/149‑1 (MQ=255)          acAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAAt                                                                                                                                           >  6:126604/2‑149 (MQ=255)            aaGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtgg                                                                                                                                         <  5:119381/149‑3 (MQ=255)              gagCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCtt                                                                                                                                       >  2:53843/1‑149 (MQ=255)               agCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                      <  5:31840/149‑1 (MQ=255)               agCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                      <  3:228582/149‑1 (MQ=255)                 cgcCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                      >  5:71471/1‑147 (MQ=255)                 cgcCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                    <  7:297842/149‑1 (MQ=255)                      aGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTTCGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACAt                                                                                                                               <  6:286989/149‑1 (MQ=255)                        gCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACAtc                                                                                                                              >  6:171909/1‑148 (MQ=255)                            ctaGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCa                                                                                                                           <  4:225716/147‑1 (MQ=255)                               gCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtggg                                                                                                                                        >  6:369139/1‑128 (MQ=255)                               gCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtggg                                                                                                                                        <  5:369139/131‑4 (MQ=255)                               gCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACAt                                                                                                                       >  5:119173/1‑148 (MQ=255)                               gCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACAt                                                                                                                       >  2:123240/1‑148 (MQ=255)                                 gAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGAc                                                                                                                    <  4:205478/149‑1 (MQ=255)                                 gAGGCCAGAAAAGCTCCCCCAGAGGAGCTCACGCTATGGAAACTTGCTAAGTATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCGTTGGAGACATCTCAACATGAc                                                                                                                    <  8:246767/149‑1 (MQ=255)                                   ggCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGAc                                                                                                                    >  1:337925/1‑147 (MQ=255)                                   ggCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTa                                                                                                                  <  5:350185/149‑1 (MQ=255)                                    gCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAg                                                                                                                 >  8:164264/1‑149 (MQ=255)                                     caaGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAg                                                                                                                 >  4:237587/3‑148 (MQ=255)                                      cAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAg                                                                                                                 >  3:68057/1‑147 (MQ=255)                                       aGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtg                                                                                                                                          <  4:216250/121‑2 (MQ=255)                                       aGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtg                                                                                                                                          >  3:216250/1‑120 (MQ=255)                                       aGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAg                                                                                                                 >  2:314735/1‑146 (MQ=255)                                       aGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAg                                                                                                              <  1:314735/149‑1 (MQ=255)                                        gCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAg                                                                                                              >  2:133325/1‑148 (MQ=255)                                             gcgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                      >  7:294510/1‑119 (MQ=255)                                             gcgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                      <  8:294510/119‑1 (MQ=255)                                              cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                       <  4:68057/149‑1 (MQ=255)                                              cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                       <  1:123240/149‑1 (MQ=255)                                              cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                       <  7:164264/149‑1 (MQ=255)                                              cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                       <  2:118014/149‑1 (MQ=255)                                              cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                       <  6:71471/149‑1 (MQ=255)                                                  cccAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCAc                                                                                                   <  6:119173/149‑1 (MQ=255)                                                  cccAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCAc                                                                                                   <  7:142189/149‑1 (MQ=255)                                                                  ctctGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGtctc                                                                                   <  5:126604/149‑1 (MQ=255)                                                                   tctGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGTGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATa                                                                                        <  2:70396/143‑1 (MQ=255)                                                                   tctGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGGGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATa                                                                                        >  1:70396/1‑143 (MQ=255)                                                                      ggAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTAt                                                                               >  8:33804/1‑149 (MQ=255)                                                                         aaCTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCACCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGtt                                                                            >  6:222163/1‑149 (MQ=255)                                                                            ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcgg                                                                                                                                                <  3:120184/78‑1 (MQ=255)                                                                            ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcgg                                                                                                                                                >  4:120184/1‑78 (MQ=255)                                                                            ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGa                                                                                                                     <  3:333263/105‑1 (MQ=255)                                                                            ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGa                                                                                                                     >  4:333263/1‑105 (MQ=255)                                                                            ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGAGAATCCTTGGAGACATATCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTgg                                                                          >  8:109964/1‑148 (MQ=255)                                                                             tCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGaa                                                                        >  8:276545/1‑149 (MQ=255)                                                                              ccAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGaa                                                                        >  7:282789/1‑148 (MQ=255)                                                                               cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGTCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGtt                                                                            <  8:171549/143‑1 (MQ=255)                                                                               cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                    >  2:64114/1‑87 (MQ=255)                                                                               cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                    <  1:64114/87‑1 (MQ=255)                                                                               cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTAGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAAt                                                                       >  1:345278/1‑148 (MQ=255)                                                                               cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGACACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGtt                                                                            >  7:171549/1‑143 (MQ=255)                                                                                aaaGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAAt                                                                       >  5:93090/1‑147 (MQ=255)                                                                                   gAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGac                                                                  <  3:149708/149‑1 (MQ=255)                                                                                   gAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGac                                                                  <  7:343322/149‑1 (MQ=255)                                                                                    aaTCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGacc                                                                 <  1:53843/149‑1 (MQ=255)                                                                                    aaTCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGac                                                                  >  6:182385/1‑148 (MQ=255)                                                                                      tCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaa                                                               <  5:171909/149‑1 (MQ=255)                                                                                         cTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCGATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTg                                                                           <  2:343882/134‑1 (MQ=255)                                                                                         cTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTg                                                                           >  1:343882/1‑134 (MQ=255)                                                                                         cTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaatac                                                            <  2:337925/149‑1 (MQ=255)                                                                                          ttACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaatac                                                            >  5:289946/1‑148 (MQ=255)                                                                                               aCCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACcaataccaatac                                                      >  7:119966/1‑149 (MQ=255)                                                                                                  tGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGc                                                   >  5:124155/1‑149 (MQ=255)                                                                                                   gCAGGAGACCATTCCACGATATAAGAGTGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                       >  1:3529/1‑97 (MQ=255)                                                                                                   gCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                       <  2:3529/96‑1 (MQ=255)                                                                                                       gagaCCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGAtt                                              >  4:343534/1‑149 (MQ=255)                                                                                                        agaCCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTg                                             <  1:133325/149‑1 (MQ=255)                                                                                                            cATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCAt                                         >  1:75851/1‑149 (MQ=255)                                                                                                            cATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGAAATCAGACCAATACCAATACTGCCGATTGCCAt                                         <  2:191158/149‑1 (MQ=255)                                                                                                                 cACGATATAAGA‑TGCAGAGGTGCACCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCaaa                                    <  5:222163/149‑1 (MQ=255)                                                                                                                      tataAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATc                               <  6:93090/149‑1 (MQ=255)                                                                                                                        taAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCATAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGt                             <  3:237587/149‑1 (MQ=255)                                                                                                                              tGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGc                                                   >  7:11043/1‑122 (MQ=255)                                                                                                                              tGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGc                                                   <  8:11043/122‑1 (MQ=255)                                                                                                                                   agGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATc                   <  6:124155/149‑1 (MQ=255)                                                                                                                                      tGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATg                                        <  2:321287/125‑1 (MQ=255)                                                                                                                                      tGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATg                                        >  1:321287/1‑125 (MQ=255)                                                                                                                                       caaggaTTCGCCGCAGGTGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCgg               >  2:17825/6‑149 (MQ=255)                                                                                                                                        ccccATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCggg              >  5:341716/1‑149 (MQ=255)                                                                                                                                        ccacATTAGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCggg              >  4:273925/4‑149 (MQ=255)                                                                                                                                          ccATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCt            >  5:254777/1‑149 (MQ=255)                                                                                                                                          ccATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTAGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCt            >  1:294783/1‑149 (MQ=255)                                                                                                                                            aTTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTg          >  2:214165/1‑149 (MQ=255)                                                                                                                                             ttCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTgc         <  3:343534/149‑1 (MQ=255)                                                                                                                                                gCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTgcgc       <  5:182385/148‑1 (MQ=255)                                                                                                                                                    ccTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCt  <  2:294783/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                   CAGGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCT  >  NC_002947/5064465‑5064759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑