Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,064,615	C→T	9.2%	A128T (GCA→ACA) ‡	PP_4461 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,064,615	0	C	T	9.2%	245.1 / 10.6	87	A128T (GCA→ACA) ‡	PP_4461	MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (39/40);  new base T (4/4);  total (43/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTT  >  NC_002947/5064467‑5064761                                                                                                                                                      |                                                                                                                                                   caaaTGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgc                                                                                                                                                    <  3:284854/147‑1 (MQ=255)  gAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcg                                                                                                                                                   >  8:343322/1‑149 (MQ=255)  gAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcg                                                                                                                                                   >  1:191158/1‑149 (MQ=255)   aaTGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcgg                                                                                                                                                  <  1:272108/149‑1 (MQ=255)    aTGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcggc                                                                                                                                                 <  7:189656/149‑1 (MQ=255)      gAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACCACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATACGCCACCTgcggcg                                                                                                                                                >  7:246767/1‑148 (MQ=255)       aGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTACACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGaa                                                                                                                                              >  1:367397/1‑149 (MQ=255)       aGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTACACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGaa                                                                                                                                              <  2:367397/149‑1 (MQ=255)        acAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAAt                                                                                                                                             >  6:126604/2‑149 (MQ=255)          aaGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtgg                                                                                                                                           <  5:119381/149‑3 (MQ=255)            gagCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCtt                                                                                                                                         >  2:53843/1‑149 (MQ=255)             agCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                        <  3:228582/149‑1 (MQ=255)             agCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                        <  5:31840/149‑1 (MQ=255)               cgcCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                        >  5:71471/1‑147 (MQ=255)               cgcCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                      <  7:297842/149‑1 (MQ=255)                    aGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTTCGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACAt                                                                                                                                 <  6:286989/149‑1 (MQ=255)                      gCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACAtc                                                                                                                                >  6:171909/1‑148 (MQ=255)                          ctaGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCa                                                                                                                             <  4:225716/147‑1 (MQ=255)                             gCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtggg                                                                                                                                          >  6:369139/1‑128 (MQ=255)                             gCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtggg                                                                                                                                          <  5:369139/131‑4 (MQ=255)                             gCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACAt                                                                                                                         >  5:119173/1‑148 (MQ=255)                             gCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACAt                                                                                                                         >  2:123240/1‑148 (MQ=255)                               gAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGAc                                                                                                                      <  4:205478/149‑1 (MQ=255)                               gAGGCCAGAAAAGCTCCCCCAGAGGAGCTCACGCTATGGAAACTTGCTAAGTATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCGTTGGAGACATCTCAACATGAc                                                                                                                      <  8:246767/149‑1 (MQ=255)                                 ggCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGAc                                                                                                                      >  1:337925/1‑147 (MQ=255)                                 ggCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTa                                                                                                                    <  5:350185/149‑1 (MQ=255)                                  gCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAg                                                                                                                   >  8:164264/1‑149 (MQ=255)                                   caaGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAg                                                                                                                   >  4:237587/3‑148 (MQ=255)                                    cAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAg                                                                                                                   >  3:68057/1‑147 (MQ=255)                                     aGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtg                                                                                                                                            >  3:216250/1‑120 (MQ=255)                                     aGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtg                                                                                                                                            <  4:216250/121‑2 (MQ=255)                                     aGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAg                                                                                                                   >  2:314735/1‑146 (MQ=255)                                     aGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAg                                                                                                                <  1:314735/149‑1 (MQ=255)                                      gCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAg                                                                                                                >  2:133325/1‑148 (MQ=255)                                           gcgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                        <  8:294510/119‑1 (MQ=255)                                           gcgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                        >  7:294510/1‑119 (MQ=255)                                            cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                         <  7:164264/149‑1 (MQ=255)                                            cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                         <  4:68057/149‑1 (MQ=255)                                            cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                         <  2:118014/149‑1 (MQ=255)                                            cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                         <  1:123240/149‑1 (MQ=255)                                            cgcCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                         <  6:71471/149‑1 (MQ=255)                                                cccAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCAc                                                                                                     <  6:119173/149‑1 (MQ=255)                                                cccAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCAc                                                                                                     <  7:142189/149‑1 (MQ=255)                                                                ctctGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGtctc                                                                                     <  5:126604/149‑1 (MQ=255)                                                                 tctGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGTGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATa                                                                                          <  2:70396/143‑1 (MQ=255)                                                                 tctGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGGGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATa                                                                                          >  1:70396/1‑143 (MQ=255)                                                                    ggAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTAt                                                                                 >  8:33804/1‑149 (MQ=255)                                                                       aaCTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCACCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGtt                                                                              >  6:222163/1‑149 (MQ=255)                                                                          ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcgg                                                                                                                                                  >  4:120184/1‑78 (MQ=255)                                                                          ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTgcgg                                                                                                                                                  <  3:120184/78‑1 (MQ=255)                                                                          ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGa                                                                                                                       <  3:333263/105‑1 (MQ=255)                                                                          ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGa                                                                                                                       >  4:333263/1‑105 (MQ=255)                                                                          ttCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGAGAATCCTTGGAGACATATCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTgg                                                                            >  8:109964/1‑148 (MQ=255)                                                                           tCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGaa                                                                          >  8:276545/1‑149 (MQ=255)                                                                            ccAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGaa                                                                          >  7:282789/1‑148 (MQ=255)                                                                             cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGTCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGtt                                                                              <  8:171549/143‑1 (MQ=255)                                                                             cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                      <  1:64114/87‑1 (MQ=255)                                                                             cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                      >  2:64114/1‑87 (MQ=255)                                                                             cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTAGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAAt                                                                         >  1:345278/1‑148 (MQ=255)                                                                             cAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGACACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGtt                                                                              >  7:171549/1‑143 (MQ=255)                                                                              aaaGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAAt                                                                         >  5:93090/1‑147 (MQ=255)                                                                                 gAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGac                                                                    <  3:149708/149‑1 (MQ=255)                                                                                 gAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGac                                                                    <  7:343322/149‑1 (MQ=255)                                                                                  aaTCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGacc                                                                   <  1:53843/149‑1 (MQ=255)                                                                                  aaTCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGac                                                                    >  6:182385/1‑148 (MQ=255)                                                                                    tCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaa                                                                 <  5:171909/149‑1 (MQ=255)                                                                                       cTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCGATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTg                                                                             <  2:343882/134‑1 (MQ=255)                                                                                       cTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTg                                                                             >  1:343882/1‑134 (MQ=255)                                                                                       cTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaatac                                                              <  2:337925/149‑1 (MQ=255)                                                                                        ttACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaatac                                                              >  5:289946/1‑148 (MQ=255)                                                                                             aCCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACcaataccaatac                                                        >  7:119966/1‑149 (MQ=255)                                                                                                tGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGc                                                     >  5:124155/1‑149 (MQ=255)                                                                                                 gCAGGAGACCATTCCACGATATAAGAGTGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                         >  1:3529/1‑97 (MQ=255)                                                                                                 gCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                         <  2:3529/96‑1 (MQ=255)                                                                                                     gagaCCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGAtt                                                >  4:343534/1‑149 (MQ=255)                                                                                                      agaCCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTg                                               <  1:133325/149‑1 (MQ=255)                                                                                                          cATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCAt                                           >  1:75851/1‑149 (MQ=255)                                                                                                          cATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGAAATCAGACCAATACCAATACTGCCGATTGCCAt                                           <  2:191158/149‑1 (MQ=255)                                                                                                               cACGATATAAGA‑TGCAGAGGTGCACCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCaaa                                      <  5:222163/149‑1 (MQ=255)                                                                                                                    tataAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATc                                 <  6:93090/149‑1 (MQ=255)                                                                                                                      taAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCATAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGt                               <  3:237587/149‑1 (MQ=255)                                                                                                                            tGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGc                                                     <  8:11043/122‑1 (MQ=255)                                                                                                                            tGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGc                                                     >  7:11043/1‑122 (MQ=255)                                                                                                                                 agGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATc                     <  6:124155/149‑1 (MQ=255)                                                                                                                                    tGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATg                                          <  2:321287/125‑1 (MQ=255)                                                                                                                                    tGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATg                                          >  1:321287/1‑125 (MQ=255)                                                                                                                                     caaggaTTCGCCGCAGGTGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCgg                 >  2:17825/6‑149 (MQ=255)                                                                                                                                      ccccATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCggg                >  5:341716/1‑149 (MQ=255)                                                                                                                                      ccacATTAGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCggg                >  4:273925/4‑149 (MQ=255)                                                                                                                                        ccATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCt              >  5:254777/1‑149 (MQ=255)                                                                                                                                        ccATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTAGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCt              >  1:294783/1‑149 (MQ=255)                                                                                                                                          aTTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTg            >  2:214165/1‑149 (MQ=255)                                                                                                                                           ttCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTgc           <  3:343534/149‑1 (MQ=255)                                                                                                                                              gCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTgcgc         <  5:182385/148‑1 (MQ=255)                                                                                                                                                  ccTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCt    <  2:294783/149‑1 (MQ=255)                                                                                                                                                      cggcgAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCttt  <  7:33804/147‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                   GGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCTCACGCTCTGGAAACTTCCAAAGAATCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGA‑TGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTT  >  NC_002947/5064467‑5064761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑