Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	416,956	G→A	5.9%	P222P (CCG→CCA)	waaG →	lipopolysaccharide glucosyltransferase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	416,956	0	G	A	5.9%	205.6 / 2.8	68	P222P (CCG→CCA)	waaG	lipopolysaccharide glucosyltransferase I
Reads supporting (aligned to +/- strand):  ref base G (32/32);  new base A (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CCGTGCGCCGGCCAATGCCGCCGAGATCCGCGCTGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGGCCGCAGCGATATCCCGCGCTTCCTGCTGGGCGCCG  >  NC_002947/416809‑417104                                                                                                                                                     |                                                                                                                                                     ccGTGCGCCGGCCAATGCCGCCGAGATCCGCGCTGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGt                                                                                                                                                     <  7:162165/149‑1 (MQ=255)    tGCGCCGGACAATGCCGCCGAGATCCGCTCTGAGTTCCGCAAGGAATTCAACCTGGGGGACGACGACCTTCTGCTGGTGAATATTGGCTCAGGATTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAg                                                                                                                                                  <  7:128860/149‑1 (MQ=255)      cgcCGGCCAATGCCGCCGAGATCCGCGCTGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGcc                                                                                                                                                <  6:112579/149‑1 (MQ=255)      cgcCGGCCAATGCCGCCGAGATCCGCGCTGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGc                                                                                                                                                 <  8:7930/148‑1 (MQ=255)          ggCCAATGCCGCCGAGATCCGCGCTGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTgc                                                                                                                                            <  1:211243/149‑1 (MQ=255)           gCCAATGCCGCCGAGATCCGCGCTGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCACGCCTCAAGGCACTGGCTGCGCTGCCGTCa                                                                                                                                                   <  1:220900/142‑1 (MQ=255)           gCCAATGCCGCCGAGATCCGCGCTGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCa                                                                                                                                                   >  2:220900/1‑141 (MQ=255)              aaTGCCGCCGAGATCCGCGCTGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCaa                                                                                                                                        <  5:197758/149‑1 (MQ=255)               ttGCGGCCGAGATCCGGGCTGAGTTCCGCAAGGAATTCAACGTGGGGGATGACGAGCTGGTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCATGCGCaaa                                                                                                                                       <  8:362373/148‑1 (MQ=255)                    gccgAGATCCGCTCTGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCAc                                                                                                                                  <  4:337104/149‑1 (MQ=255)                    gccgAGATCCGCGCTGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCAc                                                                                                                                  <  8:312405/149‑1 (MQ=255)                       gagaTCCGCGCTGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAg                                                                                                                               >  1:51153/1‑149 (MQ=255)                             cgcgCTGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGc                                                                                                                             >  3:138805/1‑145 (MQ=255)                             cgcgCTGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGc                                                                                                                             <  4:138805/145‑1 (MQ=255)                                  tGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGa                                                                                                                    >  7:112714/1‑149 (MQ=255)                                        ccGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGc                                                                                                               >  3:256063/1‑148 (MQ=255)                                          gCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCTCAAACGCACCAGGCTGATGGTGATCGGCCAg                                                                                                            <  1:437/149‑1 (MQ=255)                                          gCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCATCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAg                                                                                                            >  6:61485/1‑149 (MQ=255)                                          gCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCATCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAg                                                                                                            >  6:55777/1‑149 (MQ=255)                                                aaTTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGgacgac                                                                                                      >  6:318605/1‑149 (MQ=255)                                                        cTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGgacga                                                                                                       >  7:84593/1‑140 (MQ=255)                                                        cTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGgacga                                                                                                       <  8:84593/140‑1 (MQ=255)                                                        cTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAg                                                                                                >  6:256249/1‑147 (MQ=255)                                                        cTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAg                                                                                                <  5:256249/147‑1 (MQ=255)                                                                  acgacCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCATCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTg                                                                                    <  5:61485/149‑1 (MQ=255)                                                                  acgacCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCATCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTg                                                                                    <  5:55777/149‑1 (MQ=255)                                                                             ctgGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTAc                                                                         >  1:319455/1‑149 (MQ=255)                                                                                gTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCt                                                                      >  5:303308/1‑149 (MQ=255)                                                                                  gCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTgg                                                                    >  2:95086/1‑149 (MQ=255)                                                                                            tCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGa                                                          >  5:216221/1‑149 (MQ=255)                                                                                             cAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGAc                                                         <  2:51153/149‑1 (MQ=255)                                                                                                gCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAg                                                                                                <  4:180635/107‑1 (MQ=255)                                                                                                gCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAg                                                                                                >  3:180635/1‑107 (MQ=255)                                                                                                    cAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGt                                                    <  5:88075/147‑1 (MQ=255)                                                                                                    cAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGt                                                    >  6:88075/1‑147 (MQ=255)                                                                                                    cAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTg                                                   >  7:317026/1‑148 (MQ=255)                                                                                                     aaGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAg                                                                               >  6:72562/1‑119 (MQ=255)                                                                                                     aaGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAg                                                                               <  5:72562/119‑1 (MQ=255)                                                                                                      aGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCa                                                                                                             <  3:11005/88‑1 (MQ=255)                                                                                                      aGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCa                                                                                                             >  4:11005/1‑88 (MQ=255)                                                                                                      aGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCAATGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATCGTGATCGGCCAGGACGACCCCCACGTATTCACGCTGCACAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAg                                                >  4:295209/1‑149 (MQ=255)                                                                                                       gACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGt                                               >  3:158400/1‑149 (MQ=255)                                                                                                       gACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGt                                               >  5:107164/1‑149 (MQ=255)                                                                                                          cAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATg                                                                                                                        >  3:121500/1‑73 (MQ=255)                                                                                                          cAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATg                                                                                                                        <  4:121500/73‑1 (MQ=255)                                                                                                          cAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGAGCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTtc                                            <  3:295209/149‑1 (MQ=255)                                                                                                            aaGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTtctc                                          <  5:318605/149‑1 (MQ=255)                                                                                                                      ccGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGTCCg                                  >  8:116834/1‑147 (MQ=255)                                                                                                                      ccGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGGCCg                                  >  6:192055/1‑147 (MQ=255)                                                                                                                       cGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCt                                                                      <  4:319304/110‑1 (MQ=255)                                                                                                                       cGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCt                                                                      <  4:324763/110‑1 (MQ=255)                                                                                                                       cGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCt                                                                      >  3:319304/1‑110 (MQ=255)                                                                                                                       cGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCt                                                                      >  3:324763/1‑110 (MQ=255)                                                                                                                       cGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTTGGCGACCAGGTGCAGTTTCTCAAGGGCCg                                  >  3:353517/1‑146 (MQ=255)                                                                                                                       cGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTTGGCGACCAGGTGCAGTTTCTCAAGGGCCGCa                                <  4:353517/148‑1 (MQ=255)                                                                                                                         cA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGGCCGCAGc                              >  5:48049/1‑148 (MQ=255)                                                                                                                            gCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGGCCGCAGCGat                           <  6:216221/149‑1 (MQ=255)                                                                                                                              cTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGGCCGCAGCGatat                         >  7:46007/1‑149 (MQ=255)                                                                                                                              cTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGGCCGCAGCGatat                         >  2:257335/1‑149 (MQ=255)                                                                                                                               tCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGGCCGCAGCGATATc                        >  4:34858/1‑149 (MQ=255)                                                                                                                                      aCTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAgg                                                                                                                              <  5:275660/40‑1 (MQ=255)                                                                                                                                      aCTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAgg                                                                                                                              >  6:275660/1‑40 (MQ=255)                                                                                                                                       gaggCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGGCCGCAGCGATATCCCgcgc                  <  4:264962/145‑1 (MQ=255)                                                                                                                                          gctgcgctgcCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGGCCGCAGCGATATCCCGCGCTTc               <  6:303308/147‑1 (MQ=255)                                                                                                                                           ctgcgctgcCGGCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGGCCGCAGCGATATCCCGCGCTTCctg            <  2:211180/149‑1 (MQ=255)                                                                                                                                            tgcgctgcCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTtct                                           <  7:82461/117‑1 (MQ=255)                                                                                                                                            tgcgctgcCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTtct                                           >  8:82461/1‑117 (MQ=255)                                                                                                                                               gctgcCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGGCCGCAGCGATATCCCGCGCTTCCTGCTgg        <  8:112714/149‑1 (MQ=255)                                                                                                                                                     gTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTggg                                                                   <  4:42680/84‑1 (MQ=255)                                                                                                                                                     gTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTggg                                                                   >  3:42680/1‑84 (MQ=255)                                                                                                                                                     gTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGGCCGCAGCGATATCCCGCGCTTCCTGCTGGGCGCCg  >  6:241773/1‑149 (MQ=255)                                                                                                                                                     gTCAGCCCTGCGCAAACGCACCAGGCTGATCGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGGCCGCAGCGATATCCCGCGCTTCCTGCTGGGCGCCg  >  1:30738/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CCGTGCGCCGGCCAATGCCGCCGAGATCCGCGCTGAGTTCCGCAAGGAATTCAACCTGGGGGATGACGACCTGCTGCTGGTGCAGATTGGCTCAGGCTTCAAGACCAAAGGCGTGGACCGCA‑GCCTCAAGGCACTGGCTGCGCTGCCGTCAGCCCTGCGCAAACGCACCAGGCTGATGGTGATCGGCCAGGACGACCCCAAGGTATTCCAGCTGCAAAGCGCTACCCTGGGCTTGGGCGACCAGGTGCAGTTTCTCAAGGGCCGCAGCGATATCCCGCGCTTCCTGCTGGGCGCCG  >  NC_002947/416809‑417104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑