Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,097,206 T→A 8.9% intergenic (+197/+95) PP_mr66 → / ← acsA‑I P6/acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,097,2060TA 8.9% 106.3 / 2.6 45intergenic (+197/+95)PP_mr66/acsA‑IP6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (24/17);  new base A (2/2);  total (26/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01

ACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATT‑GCCGGGAAGGGTTTACGCC‑GAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGT  >  NC_002947/5097059‑5097349
                                                                                                                                                   |                                                                                                                                                  
acgGCAAGTGCAGCGTGCCTTGATGCACATAATGCTGACTCCGATGCTCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCtt                                                                                                                                                   <  6:180994/149‑1 (MQ=255)
acgGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTGCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCtt                                                                                                                                                   <  3:6835/149‑1 (MQ=255)
    cAAGTGCCTCGTGCCTTTCTTCACAAAATGCTGACTCCCATGCACCGAAACGCGCCGGACTTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGCCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGccc                                                                                                                                               <  6:376301/149‑1 (MQ=255)
    cAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTCTGCGGGGACGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGccc                                                                                                                                               >  7:184443/1‑149 (MQ=255)
      aGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCcgc                                                                                                                                             >  3:172495/1‑149 (MQ=255)
           aGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAATg                                                                                                                                        >  3:352109/1‑149 (MQ=255)
             cGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCAAGCCcgcttttgca                                                                                                                                      <  1:336497/149‑8 (MQ=255)
                 ccTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGa                                                                                                                                   <  8:192882/148‑1 (MQ=255)
                  cTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAgg                                                                                                                                 >  6:182088/1‑149 (MQ=255)
                         cacaAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAggg                                                                                                                           <  2:205780/148‑1 (MQ=255)
                            aaaaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGa                                                                                                                         >  4:119967/1‑147 (MQ=255)
                                   tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                               >  7:330864/1‑149 (MQ=255)
                                   tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCGAAAGCGTGACGTCAGGGGACAGCtaaa                                                                                                                 >  3:239033/1‑145 (MQ=255)
                                         cGATGCACCGAAACGGGCCGGATTTGTTTCCTGGCGCGCCGAAGCTGTTCGGGGCCGTTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                          <  8:156833/148‑1 (MQ=255)
                                               aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACg                                                                                                    >  2:208417/1‑148 (MQ=255)
                                                   aaaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTc                                                                                               >  3:295230/1‑149 (MQ=255)
                                                     aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgc                                                                                             <  5:341968/149‑1 (MQ=255)
                                                      cGGGCCGGAATTGTTGCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg                                                                                            <  4:252740/149‑1 (MQ=21)
                                                       gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCgg                                                                                           <  6:182577/149‑1 (MQ=21)
                                                        ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg                                                                                          <  1:208417/149‑1 (MQ=255)
                                                             gAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCTGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGc                                                                                     <  8:184443/149‑1 (MQ=255)
                                                                   tttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGGCAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGcgc                                                                               >  5:234692/1‑147 (MQ=255)
                                                                   tttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTc                                                                               >  7:281730/1‑149 (MQ=255)
                                                                    ttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCACGAAAGGGTGAGGTCACGGGACACCA‑TAGCCAGAACTGACCCAATAGCGGGCAAGCCCCCTc                                                                               >  4:123206/1‑148 (MQ=255)
                                                                          gcgcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAGGCGTGAGGTGAGGGGGCAGCG‑TAGCCGGAACTGACGCATTCGCGGGCAAGCCCGCTGCTGCAgg                                                                        >  7:334872/1‑149 (MQ=255)
                                                                          gcgcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGACCGCGAATTCGTGAGGTCACGGTGCAGCT‑TAGACTGAACGGCCGCCTTCTCGGGCGACGCCCCTCCTGCAgg                                                                        >  2:210501/1‑149 (MQ=255)
                                                                           cgcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGa                                                                                                                         <  2:326605/100‑1 (MQ=255)
                                                                           cgcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGa                                                                                                                         >  1:326605/1‑100 (MQ=255)
                                                                            gcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAACGCGGGCTTGCCCGCGAATGCGTGAGGGCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAgggg                                                                      <  5:182088/149‑1 (MQ=255)
                                                                              cccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAg                                                                                                                                  >  5:330249/1‑88 (MQ=255)
                                                                              cccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAg                                                                                                                                  <  6:330249/88‑1 (MQ=255)
                                                                               ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTGAGGGGGCAGCG‑TATCCTGAACTGACGCATTAGCGGGCAAGCCCGCTCCTGCAgggggga                                                                   >  7:164396/1‑147 (MQ=255)
                                                                                       gtgCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGTCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATT‑GCCgg                                                           >  2:237238/1‑148 (MQ=255)
                                                                                                       aGAACAGTAACCGATGATTGTCGGTTAACAATGCAAAAGCGGGCTTGCCCGGTAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTCAACTGACGATTTCGCGGGAAAGCCCGCTCCTGCAGGGGGTATT‑GCCGGGAAGGGTTTACGCC‑Gaa                                         <  3:119967/149‑1 (MQ=255)
                                                                                                         aaCAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATT‑GCCGGGAAGGGTTTACGCC‑GAAGc                                       >  2:85486/1‑149 (MQ=255)
                                                                                                          aCAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCTAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATT‑GCCGGGAAGGGTTTACGCC‑GAAGcc                                      <  1:75283/149‑1 (MQ=255)
                                                                                                           cAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACCCATTCGCGGGCAAGCCCCCGCCTGGAGGGGGTATT‑GCCGGGAAAGGTTTACGCC‑GAAga                                       >  4:284290/1‑146 (MQ=255)
                                                                                                                aCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTGAGGGGACAGCT‑TAGCCTGAACTGACGCATTAGCGGGCAACCCCCCGCCGGCAGGGGGGTTT‑GCCGGGAAGGGTTTACCCC‑GAAGCCCGGtt                                 >  4:159086/1‑148 (MQ=255)
                                                                                                                   gatgatTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACCCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATT‑GCCGGGAAGGGTTTACGCC‑CAAGCCACGTTCa                               >  2:263560/1‑147 (MQ=255)
                                                                                                                             gggTAACATTGAAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCCGGGCACAGCT‑TAGCCTGAACTGCCCCATTCGGGGGCAAGCCCGCTCCTGCAGGGGGTATTCGCCGGGAAGGGTTTACGCC‑GAAGCCAGGTTCATCGCCTTGTgg                    <  1:263560/149‑1 (MQ=255)
                                                                                                                                     atcaaaatGCGGGCTTCCCGGCTATTGCTTGTGGGCCGGGCACCGAT‑TCGCTTTAACTCCCGCATGCGGGGCAAAGCCCGCTCCTGCAGGGGGTATT‑GCCGGGAAGGGTTTACGCC‑GAAGCCAGGTTCATCGCCTTGTGGGTATCGATc           <  3:123206/145‑1 (MQ=255)
                                                                                                                                      tGCAAAACCGGGCTTGCCCGCTAATGCGGTCGGTCCGGGGACAGCT‑TAGTCTTAACTGCCCCATTGGGGGGCAAGCCCGCTCCTGCAGGGGGTATT‑GCCGGGAAGGGTTTACGCCGGAAGCCAGGTTCATCGCCTTGTGGGTATCGATc           <  1:237238/149‑1 (MQ=255)
                                                                                                                                        cattcgCGGGCAAGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATT‑GCCGGGAAGGGTTTACGCC‑GAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAgg        >  2:190824/6‑149 (MQ=255)
                                                                                                                                         aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATT‑GCCGGGAAGGGTTTACGCC‑GAAGCCAGGTTCATCGCCGTGTGGGTATCGATCa          >  7:199273/1‑146 (MQ=255)
                                                                                                                                         aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATT‑GCCGGGAAGGGGTTACGCC‑GAAGGCAGGTTCATCGCCTTGTGGGTATCGATCAGGt       >  2:6814/1‑149 (MQ=255)
                                                                                                                                          ttcgCGGGCAAGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATT‑GCCGGGAAGGGTTTACGCC‑GAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg      >  2:103772/4‑149 (MQ=255)
                                                                                                                                              cGGGCAAGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATT‑GCCGGGAAGGGTTTACGCC‑GAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGt  <  1:190824/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACGGCAAGTGCAGCGTGCCTTGATGCACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATT‑GCCGGGAAGGGTTTACGCC‑GAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGT  >  NC_002947/5097059‑5097349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑