Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,097,413 T→A 6.2% K660M (AAG→ATG)  acsA‑I ← acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,097,4130TA 6.2% 247.7 / 4.9 80K660M (AAG→ATG) acsA‑Iacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (38/37);  new base A (3/2);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

CCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGA  >  NC_002947/5097268‑5097557
                                                                                                                                                 |                                                                                                                                                
cccGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACCCCCGGGTCAGCCAAGGTCGAGATATCGCGCAGGGCGTCGTACTCCCGGGTGGCGATCTTgc                                                                                                                                               >  2:215239/1‑149 (MQ=255)
cccGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTgc                                                                                                                                               >  2:201168/1‑149 (MQ=255)
cccGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTgc                                                                                                                                               <  7:134732/149‑1 (MQ=255)
   gCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCa                                                                                                                                            <  2:86318/149‑1 (MQ=255)
          cAGGGGGTATAGCCGGGAAGGGTTTACGCCGAAGCCAGGATAATAGCGTTGTGGGTATCGTTCAGGTGATGTCCCACACCCGGGTCAGCCAAGCTCGACATATCGCCCAGAGGGTCGGACTCACCGGTGGAGATCTTGCGCAGGATgcg                                                                                                                                     >  6:323991/1‑149 (MQ=255)
                 tATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATg                                                                                                                              <  1:6814/149‑1 (MQ=255)
                  cttGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGa                                                                                                                             <  3:128461/148‑1 (MQ=255)
                     gCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCATGCGCCGCATCCTGCGCAAGATc                                                                                                                           <  2:291452/148‑1 (MQ=255)
                      ccGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCtt                                                                                                                         >  8:187179/1‑149 (MQ=255)
                           aaGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCg                                                                                                                    <  1:201168/149‑1 (MQ=255)
                             gggTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAg                                                                                                                  >  2:234361/1‑149 (MQ=255)
                                tttACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCgg                                                                                                               <  6:80779/149‑1 (MQ=255)
                                tttACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCgg                                                                                                               >  7:352634/1‑149 (MQ=255)
                                tttACGCCGAACCCAGGTTCATCGCCTTGGGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCgg                                                                                                               <  6:33254/149‑1 (MQ=255)
                                      ccGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTc                                                                                                         <  5:200817/149‑1 (MQ=255)
                                         aaGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGc                                                                                                                 >  6:160498/1‑138 (MQ=255)
                                         aaGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGc                                                                                                                 <  5:160498/138‑1 (MQ=255)
                                         aaGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGc                                                                                                      <  1:210501/149‑1 (MQ=255)
                                           gCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAg                                                                                                    >  3:139027/1‑149 (MQ=255)
                                            ccAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGc                                                                                                   >  4:31569/1‑149 (MQ=255)
                                            ccAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCATGCGCCGCATCCTGCGCAAGATc                                                                                                                           <  6:381356/125‑1 (MQ=255)
                                            ccAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCATGCGCCGCATCCTGCGCAAGATc                                                                                                                           >  5:381356/1‑125 (MQ=255)
                                             cAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTCCCACCCCCGGGTCAGCCAAGGTCGAGATATCGCCCCGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGGCTTCGGCAGcc                                                                                                  >  3:269317/1‑149 (MQ=255)
                                                        ccTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCAc                                                                                       >  8:296627/1‑149 (MQ=255)
                                                            gtgGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTgg                                                                                    <  3:31569/148‑1 (MQ=255)
                                                            gtgGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTg                                                                                     <  1:234361/147‑1 (MQ=255)
                                                             tgGGTATCGATCAGGGGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGa                                                                                   <  8:352634/148‑1 (MQ=255)
                                                               ggTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATc                                                                                 <  7:296627/148‑1 (MQ=255)
                                                                gTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCtt                                                                                                          >  2:367154/1‑122 (MQ=255)
                                                                gTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCtt                                                                                                          <  1:367154/122‑1 (MQ=255)
                                                                gTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATca                                                                                >  4:322050/1‑148 (MQ=255)
                                                                 tATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATcaca                                                                              <  3:188661/149‑1 (MQ=255)
                                                                         aGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGTATCACATCCGGCg                                                                       <  8:118961/148‑1 (MQ=255)
                                                                          ggTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGaa                                                                     <  3:333510/149‑1 (MQ=255)
                                                                          ggTGCTGTACCACACCCGGGTCAGCCAAGGTAGAGTTATCGCCCAGGGCGGCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGaa                                                                     <  4:269317/149‑1 (MQ=255)
                                                                           gTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAg                                                                    >  8:111019/1‑149 (MQ=255)
                                                                            tGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAg                                                                    >  1:224830/1‑148 (MQ=255)
                                                                                gTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATc                                                               >  5:326863/1‑149 (MQ=255)
                                                                                 tACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATgc                                                                                                                                      >  1:119715/1‑77 (MQ=255)
                                                                                 tACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATgc                                                                                                                                      <  2:119715/77‑1 (MQ=255)
                                                                                       acCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCgg                                                                                                               <  6:225027/94‑1 (MQ=255)
                                                                                       acCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCgg                                                                                                               >  5:225027/1‑94 (MQ=255)
                                                                                         ccGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGAtc                                                      >  5:34327/1‑149 (MQ=255)
                                                                                            ggTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGAGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTcc                                                   >  1:251364/1‑149 (MQ=255)
                                                                                             gTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCt                                                  >  4:230896/1‑149 (MQ=255)
                                                                                              tCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCtt                                                 <  2:224830/149‑1 (MQ=255)
                                                                                                aGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCTGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTgc                                               <  3:230896/149‑1 (MQ=255)
                                                                                                  ccAAGGTCGAGATATCGCCCAGGGCGTCGTACTCTCCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGACGAAGCAATCGGGCCGATCTCCTTgcgc                                             <  1:215239/149‑1 (MQ=255)
                                                                                                  ccAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGc                                                           <  3:93124/135‑1 (MQ=255)
                                                                                                  ccAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGc                                                           >  4:93124/1‑135 (MQ=255)
                                                                                                  ccAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTgcg                                              >  1:168842/1‑148 (MQ=255)
                                                                                                        tCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAg                                       <  6:34327/149‑1 (MQ=255)
                                                                                                           agaTATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGttc                                    >  8:266845/1‑149 (MQ=255)
                                                                                                            gaTATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGgcgc                                                                                           >  7:359192/1‑93 (MQ=255)
                                                                                                            gaTATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGgcgc                                                                                           <  8:359192/93‑1 (MQ=255)
                                                                                                             atatCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGttctt                                  >  7:311661/1‑149 (MQ=255)
                                                                                                               atCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTcc                                                   >  3:343843/1‑130 (MQ=255)
                                                                                                               atCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTcc                                                   <  4:343843/130‑1 (MQ=255)
                                                                                                               atCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGttctt                                  >  8:55293/1‑147 (MQ=255)
                                                                                                                  gCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGAt                                                                                  <  8:246522/96‑1 (MQ=255)
                                                                                                                  gCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGAt                                                                                  >  7:246522/1‑96 (MQ=255)
                                                                                                                   cccAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTc                            >  7:161768/1‑149 (MQ=255)
                                                                                                                      cgggCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAg                         <  8:188409/148‑1 (MQ=255)
                                                                                                                          cgtcgtACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGgcgc                     <  7:187179/149‑1 (MQ=255)
                                                                                                                           gtcgtACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCa                    <  8:161768/149‑1 (MQ=255)
                                                                                                                              gtACTCAGCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTTCCCGAGCGTGTCTTCGGCAGCCCCGGCGCCCTCTGGATCTCATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCCCCGAGTTCTTCAGCTCCAGGCGCAAt                  <  5:323991/148‑1 (MQ=255)
                                                                                                                                  tCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCTGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTc             >  5:271749/1‑149 (MQ=255)
                                                                                                                                  tCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTc             >  3:84437/1‑149 (MQ=255)
                                                                                                                                    aCCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGc           >  1:16193/1‑149 (MQ=255)
                                                                                                                                    aCCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGc           >  3:275142/1‑149 (MQ=255)
                                                                                                                                        gTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCtt                                                 <  4:126878/107‑1 (MQ=255)
                                                                                                                                        gTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCtt                                                 >  3:126878/1‑107 (MQ=255)
                                                                                                                                          ggCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCt     >  4:207646/1‑149 (MQ=255)
                                                                                                                                           gCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTc    <  8:311661/149‑1 (MQ=255)
                                                                                                                                            cGATCTTGCGCAGTATGCGGCGCATGATCTTGCCGGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAAtt                 <  3:260684/135‑1 (MQ=255)
                                                                                                                                            cGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCACCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAAtt                 >  4:260684/1‑135 (MQ=255)
                                                                                                                                             gATCATGCGCCGCATCCTGCGCAAGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGa  >  4:215688/1‑149 (MQ=255)
                                                                                                                                             gATCATGCGCCGCATCCTGCGCAAGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGa  >  1:292669/1‑149 (MQ=255)
                                                                                                                                               tCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGttcttc                                 <  4:311945/116‑1 (MQ=255)
                                                                                                                                               tCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGttcttc                                 >  3:311945/1‑116 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGCCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGGCGCAATTGCTCGCTGGCCTCGA  >  NC_002947/5097268‑5097557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑