Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,119,196 G→C 5.3% E324Q (GAG→CAG)  PP_4505 → sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,119,1960GC 5.3% 246.9 / 3.0 76E324Q (GAG→CAG) PP_4505sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (37/35);  new base C (2/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01

CCTGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGTGATCAG  >  NC_002947/5119051‑5119344
                                                                                                                                                   |                                                                                                                                                     
ccTGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAgg                                                                                                                                                    >  3:75110/1‑149 (MQ=255)
    gAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGgcg                                                                                                                                                  >  6:308072/1‑147 (MQ=255)
             aTCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGttt                                                                                                                                       <  8:298129/149‑1 (MQ=255)
                aGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAAGCCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGttt                                                                                                                                       >  1:94155/1‑147 (MQ=255)
                aGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAg                                                                                                                                    >  6:222522/1‑149 (MQ=255)
                  cGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCt                                                                                                                                  >  6:385070/1‑149 (MQ=255)
                  cGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCt                                                                                                                                  >  1:355739/1‑149 (MQ=255)
                  cGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCt                                                                                                                                  >  2:374218/1‑149 (MQ=255)
                  cGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTCCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCt                                                                                                                                  >  2:67742/1‑149 (MQ=255)
                            gCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGt                                                                                                                          <  4:75110/147‑1 (MQ=255)
                            gCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGt                                                                                                                          <  2:243312/147‑1 (MQ=255)
                             cccTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGttt                                                                                                                                       <  8:168055/133‑1 (MQ=255)
                             cccTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGttt                                                                                                                                       >  7:168055/1‑133 (MQ=255)
                                        gTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGAt                                                                                                            <  2:355739/149‑1 (MQ=255)
                                           gATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATc                                                                                                           >  3:151326/1‑147 (MQ=255)
                                             tGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGc                                                                                                       >  7:327289/1‑149 (MQ=255)
                                                   aCAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAgg                                                                                                                            <  1:348982/122‑1 (MQ=255)
                                                   aCAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCCCCAGGAAGCGCAGCCAGGTTTTAGCATTCAgg                                                                                                                            >  2:348982/1‑122 (MQ=255)
                                                     aaGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGtt                                                                                               <  5:222522/149‑1 (MQ=255)
                                                      aGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTg                                                                                              <  1:374218/149‑1 (MQ=255)
                                                       gCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGa                                                                                             <  2:133374/149‑1 (MQ=255)
                                                         ccATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGAc                                                                                            >  3:46456/1‑148 (MQ=255)
                                                          cATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGtt                                                                                                                         >  6:232667/1‑118 (MQ=255)
                                                          cATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGtt                                                                                                                         <  5:232667/118‑1 (MQ=255)
                                                           aTGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACacc                                                                                         <  1:102753/149‑3 (MQ=255)
                                                              ccGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACctg                                                                                      <  5:385070/149‑1 (MQ=255)
                                                              ccGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACct                                                                                       <  5:308072/148‑1 (MQ=255)
                                                                 aa‑CCGGTAGACCTAAATGACCTACTCGGCAGAGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGGGAGGAGGCGCAGCCAGGGTTTACGATGCAGGGTAGGCCAACGCTAATCGAGCGTGCCGTTGACAAActgctg                                                                                   >  6:233303/1‑149 (MQ=255)
                                                                  a‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCtgctgc                                                                                  <  2:96007/149‑1 (MQ=255)
                                                                       gTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCaa                                                                              >  4:256714/1‑149 (MQ=255)
                                                                        tCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTg                                                                                              >  4:333107/1‑132 (MQ=255)
                                                                        tCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTg                                                                                              <  3:333107/132‑1 (MQ=255)
                                                                          gACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCCGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGc                                                                          >  1:377761/1‑149 (MQ=255)
                                                                          gACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGc                                                                          >  2:308993/1‑149 (MQ=255)
                                                                             cTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCt                                                                       >  7:111577/1‑149 (MQ=255)
                                                                                 aTGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTTAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTgcgc                                                                   <  6:247976/149‑1 (MQ=255)
                                                                                    cccTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGcc                                                                         >  2:120006/1‑140 (MQ=255)
                                                                                    cccTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGCAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGcc                                                                         <  1:120006/140‑1 (MQ=255)
                                                                                      cTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGc                                                                                                                      >  1:258109/1‑94 (MQ=255)
                                                                                      cTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGc                                                                                                                      <  2:258109/94‑1 (MQ=255)
                                                                                      cTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCAGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCaa                                                              >  8:131748/1‑149 (MQ=255)
                                                                                       tACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGGAATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGt                                                                                                     >  1:265067/1‑111 (MQ=255)
                                                                                       tACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGgcgga                                                                                                                                                >  1:113769/1‑65 (MQ=255)
                                                                                       tACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGgcgc                                                                                                                                                 <  2:113769/66‑1 (MQ=255)
                                                                                       tACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGt                                                                                                     <  2:265067/110‑1 (MQ=255)
                                                                                          tCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCcg                                                          >  3:195292/1‑149 (MQ=255)
                                                                                            ggCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCcggc                                                        >  4:43904/1‑149 (MQ=255)
                                                                                             gCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCcggcg                                                       <  7:261418/149‑1 (MQ=255)
                                                                                              cAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCcggcgg                                                      <  8:327289/149‑1 (MQ=255)
                                                                                                  gTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGc                                                    >  2:229465/1‑147 (MQ=255)
                                                                                                  gTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCa                                                  >  4:142474/1‑149 (MQ=255)
                                                                                                  gTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCa                                                  <  4:354741/149‑1 (MQ=255)
                                                                                                   tGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAg                                                 >  2:99858/1‑149 (MQ=255)
                                                                                                    gcgcAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGc                                                >  8:13140/1‑149 (MQ=255)
                                                                                                           gACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGa                                         <  3:142474/149‑1 (MQ=255)
                                                                                                            aCGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAg                                        <  4:151326/149‑1 (MQ=255)
                                                                                                            aCGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAg                                        <  2:51004/149‑1 (MQ=255)
                                                                                                            aCGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAg                                        <  3:256714/149‑1 (MQ=255)
                                                                                                             cggccTGCTGAGCGCGCTGG‑ATCAGGACGTGCGGCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAAGCTGCTGCGCAA‑TGGCCTGCGCTTCAACCCGGCGGTCCAGCCGGTCGAg                                        <  6:116419/145‑1 (MQ=255)
                                                                                                             cGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAACTGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAg                                        <  1:67742/149‑1 (MQ=255)
                                                                                                              gCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGt                                      >  6:129658/1‑149 (MQ=255)
                                                                                                                ccTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGt                                      >  5:12486/1‑147 (MQ=255)
                                                                                                                       aGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGcgccgc                             <  7:13140/149‑1 (MQ=255)
                                                                                                                              cGG‑ATCAGGACGTGCGCCCGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAg                      >  5:232440/1‑149 (MQ=255)
                                                                                                                               gg‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGc                     <  4:46456/149‑1 (MQ=255)
                                                                                                                                  aTCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTGGGCCATCGCTGATCGAGCGTGCCGTTGACATCCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCTGTCGAGGTCAGCGCCGCGCGCGAGCAg                   <  5:233303/149‑1 (MQ=255)
                                                                                                                                     aGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGAc                <  1:308993/149‑1 (MQ=255)
                                                                                                                                      ggACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCcggcgg                                                      >  4:85888/1‑110 (MQ=255)
                                                                                                                                      ggACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCcggcgg                                                      <  3:85888/110‑1 (MQ=255)
                                                                                                                                      ggACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGAcc               >  3:335761/1‑149 (MQ=255)
                                                                                                                                      ctggctGCGCCTCCAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGAcc               >  7:53988/6‑149 (MQ=255)
                                                                                                                                          ctGCGCCTCCAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGAc                >  8:103119/2‑144 (MQ=255)
                                                                                                                                          ctGCGCCTCCAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGAc                <  7:103119/143‑1 (MQ=255)
                                                                                                                                           ccgatcTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATc          <  7:96397/144‑1 (MQ=255)
                                                                                                                                            gcgcCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCgtgtga         >  5:366896/1‑144 (MQ=255)
                                                                                                                                                cTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGTGAt     <  7:131748/149‑1 (MQ=255)
                                                                                                                                                   gAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATc          >  8:96397/1‑141 (MQ=255)
                                                                                                                                                   gAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGTGATCAg  >  7:129996/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCTGGAAGACCTGATCAGCGAAATCCTCGCCCTGGCCCGGGTGGATGCCGAACAAGCCCATGCCGAA‑CCGGTCGACCTCAATGCCCTACTCGGCAGCGTGCGCAAGGACGCCCTGCTGAGCGCGCCGG‑ATCAGGACGTGCGCCTGGAGGCGCAGCCAGGTTTGAGCTTGCAGGGTTGGCCAACGCTGATCGAGCGTGCCGTTGACAACCTGCTGCGCAA‑TGCCCTGCGCTTCAACCCGGCGGGCCAGCCGGTCGAGGTCAGCGCCGCGCGCGAGCAGGACCGCATCGTGATCAG  >  NC_002947/5119051‑5119344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑