Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,139,134 T→C 7.4% C76R (TGC→CGC)  PP_4524 → sodium‑solute symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,139,1340TC 7.4% 153.7 / 2.5 54C76R (TGC→CGC) PP_4524sodium‑solute symporter
Reads supporting (aligned to +/- strand):  ref base T (23/27);  new base C (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

GAAAACCCATGAAGACTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCT  >  NC_002947/5138986‑5139280
                                                                                                                                                    |                                                                                                                                                  
gAAAACGCATGAAGACTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCtgt                                                                                                                                                    <  5:367001/149‑1 (MQ=255)
gAAAACCCATGAAGACTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCTCCACAGTACTCTGTTGCGCTTCCACCGTCGGCACCTTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGACtgt                                                                                                                                                    <  5:270638/149‑1 (MQ=255)
gAAAACCCATGAAGACTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCtgt                                                                                                                                                    <  7:193511/149‑1 (MQ=255)
     ccgaTGAAGACTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGGATTTCCGGCTTCTGGCTGTGCGCa                                                                                                                                               <  2:78886/146‑1 (MQ=255)
          gAAGACTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCcct                                                                                                                                          >  4:269868/1‑149 (MQ=255)
             gACTACGTGGTTGCCGGGCGCAATCTCGGCCCGGTGCTGTAGATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCccttg                                                                                                                                        <  7:331043/148‑3 (MQ=255)
              aCTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCcctgggc                                                                                                                                      <  2:82892/149‑1 (MQ=255)
               cTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGCGCACAGccagaagcc                                                                                                                                     <  3:219976/149‑8 (MQ=255)
                  ccTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCcctgggcctgg                                                                                                                                  <  3:269868/149‑1 (MQ=255)
                  ccTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCcctgggcctgg                                                                                                                                  <  2:276506/149‑1 (MQ=255)
                   cTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCcctgggcctggg                                                                                                                                 >  8:164117/1‑149 (MQ=255)
                      gTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCctgggcctgggc                                                                                                                                <  8:229645/147‑1 (MQ=255)
                      gTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGcat                                                                                                                              >  5:289319/1‑149 (MQ=255)
                      gTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCCCAGGCCCAGGGCTGCGcac                                                                                                                              <  4:271263/149‑2 (MQ=255)
                         gCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGcatcat                                                                                                                           >  5:223929/1‑149 (MQ=255)
                            gggCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCtgt                                                                                                                                                    >  1:275583/1‑121 (MQ=255)
                            gggCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCtgt                                                                                                                                                    <  2:275583/121‑1 (MQ=255)
                                    tctcGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCa                                                                                                                      >  1:214267/1‑143 (MQ=255)
                                    tctcGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCa                                                                                                                      <  2:214267/143‑1 (MQ=255)
                                          cccGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTcc                                                                                                          >  2:12356/1‑149 (MQ=255)
                                            cGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTc                                                                                                        <  4:18962/149‑1 (MQ=255)
                                             ggTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCg                                                                                                       >  5:74788/1‑149 (MQ=255)
                                             ggTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCACCATCAACCTGTTCCTCg                                                                                                       <  7:164117/149‑1 (MQ=255)
                                              gTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGc                                                                                                      <  2:18684/149‑1 (MQ=255)
                                                    tACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCaaa                                                                                                  <  6:74788/147‑1 (MQ=255)
                                                     aCATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGCGCACAGccagaa                                                                                                                                        <  7:333232/108‑5 (MQ=255)
                                                     aCATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGCGCACAGccagaa                                                                                                                                        >  8:333232/1‑104 (MQ=255)
                                                     aCATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGCGCACAGccagaa                                                                                                                                        >  2:320444/1‑104 (MQ=255)
                                                      cATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGcatca                                                                                                                            <  1:136628/119‑1 (MQ=255)
                                                      cATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGcatca                                                                                                                            >  2:136628/1‑119 (MQ=255)
                                                       aTGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAAc                                                                                                                 >  7:221441/1‑129 (MQ=255)
                                                       aTGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAAc                                                                                                                 <  8:221441/129‑1 (MQ=255)
                                                        tGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCgctg                                                                                            <  5:136512/149‑1 (MQ=255)
                                                         gggCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCgctgc                                                                                           >  4:140476/1‑149 (MQ=255)
                                                           gCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGctgctg                                                                                         <  6:223929/149‑1 (MQ=255)
                                                               cATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGctgcgcc                                                                                     >  4:54557/1‑149 (MQ=255)
                                                                   gCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCAGCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGctgcgcctgc                                                                                  <  3:283975/148‑1 (MQ=255)
                                                                   gCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGctgcgcctgc                                                                                  <  1:169542/148‑1 (MQ=255)
                                                                      accaccGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCAt                                                                              >  6:131067/1‑149 (MQ=255)
                                                                            gTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCAc                                                                        <  5:131067/149‑1 (MQ=255)
                                                                              aCTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCg                                                                      >  5:289999/1‑149 (MQ=255)
                                                                                tCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCCTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGt                                                                     >  3:200338/1‑148 (MQ=255)
                                                                                    tGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGAccc                                                                <  6:289999/149‑1 (MQ=255)
                                                                                       cgcTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAgg                                                             <  3:54557/149‑1 (MQ=255)
                                                                                            ccACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAg                                                              >  8:326385/1‑143 (MQ=255)
                                                                                            ccACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAg                                                              <  7:326385/143‑1 (MQ=255)
                                                                                              aCCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCCTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGa                                                      <  4:200338/149‑1 (MQ=255)
                                                                                                     gCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGc                                               <  1:12356/149‑1 (MQ=255)
                                                                                                               ccTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCa                                     <  6:289319/149‑1 (MQ=255)
                                                                                                                      tACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCAGCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGTCCCAGGTACTGGAGCAACGCTACAACCCCACCGCACg                              >  1:293714/1‑149 (MQ=255)
                                                                                                                          tCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACgccag                          >  3:268463/1‑149 (MQ=255)
                                                                                                                          tCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGCACTGGAGCAACGCTACAACCCCACCGCACgccag                          >  7:347578/1‑149 (MQ=255)
                                                                                                                           ccACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCATCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCCCgccagg                         >  1:168668/1‑149 (MQ=255)
                                                                                                                            cACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCCTCACCGTGACCCAGGTACTGGAg                                                     <  7:203207/120‑1 (MQ=255)
                                                                                                                            cACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCCTCACCGTGACCCAGGTACTGGAg                                                     >  8:203207/1‑120 (MQ=255)
                                                                                                                               ggCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACgccaggccag                     >  6:24112/1‑149 (MQ=255)
                                                                                                                                      ccGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGt               >  5:353252/1‑148 (MQ=255)
                                                                                                                                                gCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGc       <  4:268463/146‑1 (MQ=255)
                                                                                                                                                gCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTgg    >  7:72450/1‑149 (MQ=255)
                                                                                                                                                  tgtgCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCt  >  4:127623/1‑149 (MQ=255)
                                                                                                                                                    tgCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCt  <  3:140476/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GAAAACCCATGAAGACTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCT  >  NC_002947/5138986‑5139280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑