Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,139,137 G→A 7.2% A77T (GCA→ACA)  PP_4524 → sodium‑solute symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,139,1370GA 7.2% 162.5 / 2.3 56A77T (GCA→ACA) PP_4524sodium‑solute symporter
Reads supporting (aligned to +/- strand):  ref base G (24/27);  new base A (2/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

CCCATGAAGACTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATG  >  NC_002947/5138991‑5139284
                                                                                                                                                  |                                                                                                                                                   
ccgaTGAAGACTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGGATTTCCGGCTTCTGGCTGTGCGCa                                                                                                                                                   <  2:78886/146‑1 (MQ=255)
     gAAGACTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCcct                                                                                                                                              >  4:269868/1‑149 (MQ=255)
        gACTACGTGGTTGCCGGGCGCAATCTCGGCCCGGTGCTGTAGATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCccttg                                                                                                                                            <  7:331043/148‑3 (MQ=255)
         aCTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCcctgggc                                                                                                                                          <  2:82892/149‑1 (MQ=255)
          cTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGCGCACAGccagaagcc                                                                                                                                         <  3:219976/149‑8 (MQ=255)
             ccTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCcctgggcctgg                                                                                                                                      <  3:269868/149‑1 (MQ=255)
             ccTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCcctgggcctgg                                                                                                                                      <  2:276506/149‑1 (MQ=255)
              cTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCcctgggcctggg                                                                                                                                     >  8:164117/1‑149 (MQ=255)
                 gTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCctgggcctgggc                                                                                                                                    <  8:229645/147‑1 (MQ=255)
                 gTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGcat                                                                                                                                  >  5:289319/1‑149 (MQ=255)
                 gTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCCCAGGCCCAGGGCTGCGcac                                                                                                                                  <  4:271263/149‑2 (MQ=255)
                    gCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGcatcat                                                                                                                               >  5:223929/1‑149 (MQ=255)
                               tctcGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCa                                                                                                                          >  1:214267/1‑143 (MQ=255)
                               tctcGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCa                                                                                                                          <  2:214267/143‑1 (MQ=255)
                                     cccGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTcc                                                                                                              >  2:12356/1‑149 (MQ=255)
                                       cGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTc                                                                                                            <  4:18962/149‑1 (MQ=255)
                                        ggTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCg                                                                                                           >  5:74788/1‑149 (MQ=255)
                                        ggTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCACCATCAACCTGTTCCTCg                                                                                                           <  7:164117/149‑1 (MQ=255)
                                         gTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGc                                                                                                          <  2:18684/149‑1 (MQ=255)
                                               tACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCaaa                                                                                                      <  6:74788/147‑1 (MQ=255)
                                                aCATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGCGCACAGccagaa                                                                                                                                            <  7:333232/108‑5 (MQ=255)
                                                aCATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGCGCACAGccagaa                                                                                                                                            >  8:333232/1‑104 (MQ=255)
                                                aCATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGCGCACAGccagaa                                                                                                                                            >  2:320444/1‑104 (MQ=255)
                                                 cATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGcatca                                                                                                                                >  2:136628/1‑119 (MQ=255)
                                                 cATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGcatca                                                                                                                                <  1:136628/119‑1 (MQ=255)
                                                  aTGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAAc                                                                                                                     >  7:221441/1‑129 (MQ=255)
                                                  aTGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAAc                                                                                                                     <  8:221441/129‑1 (MQ=255)
                                                   tGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCgctg                                                                                                <  5:136512/149‑1 (MQ=255)
                                                    gggCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCgctgc                                                                                               >  4:140476/1‑149 (MQ=255)
                                                      gCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGctgctg                                                                                             <  6:223929/149‑1 (MQ=255)
                                                          cATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGctgcgcc                                                                                         >  4:54557/1‑149 (MQ=255)
                                                              gCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCAGCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGctgcgcctgc                                                                                      <  3:283975/148‑1 (MQ=255)
                                                              gCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGctgcgcctgc                                                                                      <  1:169542/148‑1 (MQ=255)
                                                                 accaccGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCAt                                                                                  >  6:131067/1‑149 (MQ=255)
                                                                       gTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCAc                                                                            <  5:131067/149‑1 (MQ=255)
                                                                         aCTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCg                                                                          >  5:289999/1‑149 (MQ=255)
                                                                           tCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCCTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGt                                                                         >  3:200338/1‑148 (MQ=255)
                                                                               tGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGAccc                                                                    <  6:289999/149‑1 (MQ=255)
                                                                                  cgcTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAgg                                                                 <  3:54557/149‑1 (MQ=255)
                                                                                       ccACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAg                                                                  >  8:326385/1‑143 (MQ=255)
                                                                                       ccACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAg                                                                  <  7:326385/143‑1 (MQ=255)
                                                                                         aCCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCCTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGa                                                          <  4:200338/149‑1 (MQ=255)
                                                                                                gCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGc                                                   <  1:12356/149‑1 (MQ=255)
                                                                                                          ccTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCa                                         <  6:289319/149‑1 (MQ=255)
                                                                                                                 tACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCAGCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGTCCCAGGTACTGGAGCAACGCTACAACCCCACCGCACg                                  >  1:293714/1‑149 (MQ=255)
                                                                                                                     tCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACgccag                              >  3:268463/1‑149 (MQ=255)
                                                                                                                     tCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGCACTGGAGCAACGCTACAACCCCACCGCACgccag                              >  7:347578/1‑149 (MQ=255)
                                                                                                                      ccACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCATCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCCCgccagg                             >  1:168668/1‑149 (MQ=255)
                                                                                                                       cACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCCTCACCGTGACCCAGGTACTGGAg                                                         <  7:203207/120‑1 (MQ=255)
                                                                                                                       cACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCCTCACCGTGACCCAGGTACTGGAg                                                         >  8:203207/1‑120 (MQ=255)
                                                                                                                          ggCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACgccaggccag                         >  6:24112/1‑149 (MQ=255)
                                                                                                                                 ccGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGt                   >  5:353252/1‑148 (MQ=255)
                                                                                                                                           gCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGc           <  4:268463/146‑1 (MQ=255)
                                                                                                                                           gCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTgg        >  7:72450/1‑149 (MQ=255)
                                                                                                                                             tgtgCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCt      >  4:127623/1‑149 (MQ=255)
                                                                                                                                               tgCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCt      <  3:140476/147‑1 (MQ=255)
                                                                                                                                                  gcAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATg  >  3:11908/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCCATGAAGACTACCTGGTAGCCGGGCGCAATCTCGGCCCGGTGCTGTACATGGGCACCATGGCCACCACCGTACTCGGTGGCGCTTCCACCGTCGGCACCGTGCGCCTGGGCTACGTCCACGGCATTTCCGGCTTCTGGCTGTGCGCAGCCCTGGGCCTGGGCATCATCGCCATCAACCTGTTCCTCGCCAAACCGCTGCTGCGCCTGCGCATCTTCACCGTGACCCAGGTACTGGAGCAACGCTACAACCCCACCGCACGCCAGGCCAGCGCCGTCATCATGCTGGCTTATG  >  NC_002947/5138991‑5139284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑