Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,194,887 T→G 9.4% intergenic (+29/+24) PP_4574 → / ← PP_4575 hypothetical protein/urea amidolyase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,194,8870TG 9.4% 144.1 / 6.4 53intergenic (+29/+24)PP_4574/PP_4575hypothetical protein/urea amidolyase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (27/21);  new base G (2/3);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

AAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAGCG  >  NC_002947/5194742‑5195035
                                                                                                                                                 |                                                                                                                                                      
caGCAGGCCTGGGACTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGc                                                                                                                                                     <  8:152672/148‑1 (MQ=255)
  gCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGccc                                                                                                                                                   <  4:359344/149‑1 (MQ=255)
      gCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGa                                                                                                                                               <  2:20039/149‑1 (MQ=255)
          gggAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAgg                                                                                                                                           <  6:315818/149‑1 (MQ=255)
          gggAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCATTCGCGGGCTCGCCCGCGaaacg                                                                                                                                           >  1:175482/1‑147 (MQ=255)
           ggAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAgg                                                                                                                                           <  7:136452/148‑1 (MQ=255)
            gAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGc                                                                                                                                         >  3:163044/1‑149 (MQ=255)
            gAGTACAAGTCCTCGACCCATCTGTTGCGCTAGCTGATCTTGGACAAGATGTATCCGCTGACACTTCAAGGGTTGGAGTACGTGATGGGCTAACTGTCCAAGTGAGGTTGCCTTTACCTGCCCTTTCGCTGGCTCGCCCGCTaaatgtc                                                                                                                                         <  8:101510/149‑5 (MQ=255)
                  acCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCcagcag                                                                                                                                   >  2:210446/1‑149 (MQ=255)
                  acCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCcagcag                                                                                                                                   >  8:352182/1‑149 (MQ=255)
                  acCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCCAAAGGGCcagta                                                                                                                                    >  1:367345/1‑146 (MQ=255)
                    cGCCTCTACCAATTTGGTGCGCTACTTTTTCGAGTACATTATGTTTCGGCTTACCCTGAATGGTTTGTAGGAGGGTATGCGCTACTTGTCAATGTGAGGTTGCCTGTACGGGCCTTTTCGCGGGCTCGCCCGCTAAAGGGCcagtacag                                                                                                                                 <  2:367345/149‑6 (MQ=255)
                        tCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAACGGGCCAGCCGTCGTAc                                                                                                                             >  8:71430/1‑149 (MQ=255)
                                       cgcgACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAATTGAGGTTGCCTTTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAg                                                                                                               <  4:163044/148‑1 (MQ=255)
                                        gcgACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCGAGCCCGCGaaa                                                                                                                                             <  3:117616/117‑1 (MQ=255)
                                        gcgACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCGAGCCCGCGaaa                                                                                                                                             >  4:117616/1‑117 (MQ=255)
                                           aCCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTaaa                                                                                                          <  1:210446/149‑1 (MQ=255)
                                               gATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAgctg                                                                                                      >  8:74555/1‑149 (MQ=255)
                                                 tCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAgctgct                                                                                                    <  7:74555/149‑1 (MQ=255)
                                                 tCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAgctgc                                                                                                     <  7:75562/148‑1 (MQ=255)
                                                 tCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCGAGCCCGCGaaa                                                                                                                                             <  2:84379/108‑1 (MQ=255)
                                                         aaGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGc                                                                                            >  1:337685/1‑149 (MQ=255)
                                                           gATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTa                                                                                                                              <  7:287590/113‑1 (MQ=255)
                                                           gATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTa                                                                                                                              >  8:287590/1‑113 (MQ=255)
                                                           gATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCa                                                                                                                >  6:67078/1‑127 (MQ=255)
                                                           gATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGc                                                                                            >  1:149932/1‑147 (MQ=255)
                                                                aTCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCt                                                                                     >  8:164142/1‑149 (MQ=255)
                                                                aTCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCt                                                                                     >  3:301369/1‑149 (MQ=255)
                                                                          ccTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTACTGc‑‑ac                                                                         >  5:283880/1‑149 (MQ=255)
                                                                               aaGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCg                                                                    >  3:169224/1‑149 (MQ=255)
                                                                               aaGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCg                                                                    <  7:352182/149‑1 (MQ=255)
                                                                                 gggTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGcc                                                                  <  4:301369/149‑1 (MQ=255)
                                                                                         ggaCGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGc                                                                             <  6:78505/132‑1 (MQ=255)
                                                                                         ggaCGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGc                                                                             >  5:78505/1‑132 (MQ=255)
                                                                                          gaCGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGAc                                                                                                                          <  8:281768/86‑1 (MQ=255)
                                                                                          gaCGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGAc                                                                                                                          >  7:281768/1‑86 (MQ=255)
                                                                                               gATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGcaccac                                                    >  3:31454/1‑149 (MQ=255)
                                                                                                            tCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATg                                       >  8:83728/1‑149 (MQ=255)
                                                                                                                aGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACt                                   >  7:340259/1‑149 (MQ=255)
                                                                                                                      gTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCTCGCGGGCCACCGCCCGGCACCGCCCCCCCCACCCCGGCAGGCACAgcgc                               >  1:316302/1‑149 (MQ=255)
                                                                                                                           cTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgc                        <  7:83728/149‑1 (MQ=255)
                                                                                                                             ggaCCCGCCCGTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgc                      <  8:352029/147‑1 (MQ=255)
                                                                                                                              tACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgc                      <  7:164142/148‑1 (MQ=255)
                                                                                                                                 cGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgccagc                  >  3:68325/1‑149 (MQ=255)
                                                                                                                                  ggCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGca                                                        <  8:41453/110‑1 (MQ=255)
                                                                                                                                  ggCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGca                                                        >  7:41453/1‑110 (MQ=255)
                                                                                                                                  ggCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgccagcg                 >  3:130648/1‑149 (MQ=255)
                                                                                                                                   gCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCACAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgccagcgc                >  8:75062/1‑149 (MQ=255)
                                                                                                                                       tttCGCGGGCGAGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgccagcgcca              >  3:106957/1‑147 (MQ=255)
                                                                                                                                       tttCGCGGGCGAGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgccagcgc                <  4:106957/145‑1 (MQ=255)
                                                                                                                                           gcgGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAgcggcg        <  4:130648/149‑1 (MQ=255)
                                                                                                                                            cgGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGt       >  3:13571/1‑149 (MQ=255)
                                                                                                                                              ggCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGt       >  7:92079/1‑147 (MQ=255)
                                                                                                                                                 tCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAgcg  >  7:29519/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                      
AAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGC‑‑ACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAGCG  >  NC_002947/5194742‑5195035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑