Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,195,989 C→T 8.8% V176I (GTA→ATA)  ybgJ ← allophanate hydrolase subunit 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,195,9890CT 8.8% 196.4 / 6.6 68V176I (GTA→ATA) ybgJallophanate hydrolase subunit 1
Reads supporting (aligned to +/- strand):  ref base C (31/31);  new base T (4/2);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01

CCACCCATGGCCAGGAATTCGCTGCGCGAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACAC  >  NC_002947/5195847‑5196136
                                                                                                                                               |                                                                                                                                                    
ccaccCATGGCCAGGAATTCGCTGCGCGAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCgg                                                                                                                                                <  1:247323/149‑1 (MQ=255)
     cATGGCCAGGAATTCGCTGCGCGAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAgg                                                                                                                                           <  1:139613/149‑1 (MQ=255)
      aTGGCCAGGAATTCGCTGCGCGAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGgc                                                                                                                                          >  5:87642/1‑149 (MQ=255)
          ccAGGAATTCGCTGCGCGAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGgccgcc                                                                                                                                      <  4:19127/149‑1 (MQ=255)
               aaTTCGCTGCGCGAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTg                                                                                                                                 <  6:87642/149‑1 (MQ=255)
                 ttCGCTGCGCGAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGCGAAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTg                                                                                                                                 >  1:274808/1‑148 (MQ=255)
                 ttCGCTGCGCGAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTg                                                                                                                                 <  2:274808/147‑1 (MQ=255)
                 ttCGCTGCGCGAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGgc                                                                                                                               >  6:352181/1‑149 (MQ=255)
                      tGCGCGAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCg                                                                                                                          >  2:122076/1‑149 (MQ=255)
                        cgcgAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCgg                                                                                                                         <  3:255220/148‑1 (MQ=255)
                        cgcgAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCgg                                                                                                                         <  5:324095/148‑1 (MQ=255)
                        cgcgAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCg                                                                                                                          <  3:9191/147‑1 (MQ=255)
                          cgAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCaa                                                                                                                      <  5:340696/149‑1 (MQ=255)
                            aaaCCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATa                                                                                                                    <  5:306710/149‑1 (MQ=255)
                                cGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGccgccg                                                                                                                                     >  4:281186/1‑128 (MQ=255)
                                cGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGccgccg                                                                                                                                     <  3:281186/128‑1 (MQ=255)
                                 ggCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACTCTCACGGTCGAACAACCGAACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGTCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCAc                                                                                                               <  2:363560/149‑1 (MQ=255)
                                  gCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACg                                                                                                              >  7:238735/1‑149 (MQ=255)
                                     cGAAGCGCAGCCGGTCACCGGGTTGC‑AAGAGGGTGTATCGCTGACGCTCACGGTCGACCAACCGCCCCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTg                                                                                                           <  4:307681/149‑1 (MQ=255)
                                      gAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCACGGCAATACCCACGCTGc                                                                                                          >  1:281837/1‑149 (MQ=255)
                                        ttgcaaCCCGGTGACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGccc                                                                                                        >  2:75241/6‑149 (MQ=255)
                                              cccGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCcgccgc                                                                                                   >  3:101085/1‑148 (MQ=255)
                                                  gTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCAcgc                                                                                              >  8:230145/1‑149 (MQ=255)
                                                     aCCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCTGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCt                                                                                           >  1:198360/1‑149 (MQ=255)
                                                     aCCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCgg                                                                                                                         <  3:139173/119‑1 (MQ=255)
                                                     aCCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCgg                                                                                                                         >  4:139173/1‑119 (MQ=255)
                                                              c‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCg                                                                                                                                                 >  8:4043/1‑86 (MQ=255)
                                                              c‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCg                                                                                                                                                 <  7:4043/86‑1 (MQ=255)
                                                                   agGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACt                                                                              <  8:238735/149‑1 (MQ=255)
                                                                    ggCTGTAACCCTCCCGCTCACGGGCGAACCACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTc                                                                             <  2:312548/149‑1 (MQ=255)
                                                                       tGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACt                                                                              <  3:144417/145‑1 (MQ=255)
                                                                       tGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACt                                                                              >  4:144417/1‑145 (MQ=255)
                                                                        gTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGAc                                                                         >  6:138965/1‑149 (MQ=255)
                                                                         tAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGAc                                                                         >  6:35197/1‑148 (MQ=255)
                                                                             ccTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAg‑gg                                                                   >  4:296018/1‑149 (MQ=255)
                                                                                 actctcacgGTCGAACAACCGCAGCGGGGTGCGCCCGATCTGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTg                                                                <  2:281837/145‑1 (MQ=255)
                                                                                 acgctcacgGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTg                                                                <  5:138965/148‑1 (MQ=255)
                                                                                  cgctcacgGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGGGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGc                                                              <  1:75241/149‑1 (MQ=255)
                                                                                     tcacgGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAg                                                           >  7:271906/1‑149 (MQ=255)
                                                                                             gAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATa                                                                                                                    <  2:312005/85‑1 (MQ=255)
                                                                                             gAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAGCGCCTGGCCAgccctcgt                                                    <  3:160767/149‑8 (MQ=255)
                                                                                               acaacCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCtcgtc                                                  >  7:52850/1‑148 (MQ=255)
                                                                                               acaacCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCtcgtc                                                  >  7:187365/1‑148 (MQ=255)
                                                                                                 aacCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGAc                                               >  3:165688/1‑149 (MQ=255)
                                                                                                   ccGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGAc                                               >  7:327532/1‑147 (MQ=255)
                                                                                                        ccGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGccc                                        <  8:271906/149‑1 (MQ=255)
                                                                                                         cGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCa                                       >  2:340149/1‑149 (MQ=255)
                                                                                                                  cccGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCg                               >  5:136944/1‑148 (MQ=255)
                                                                                                                     gATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAAc                           >  7:136928/1‑149 (MQ=255)
                                                                                                                          ggTTCCAGCCACCCGGCGATACTGCCGGGTAGGCTGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGt                      <  2:198360/149‑1 (MQ=255)
                                                                                                                          ggTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGt                      <  8:327532/149‑1 (MQ=255)
                                                                                                                          ggTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGt                      <  6:136944/149‑1 (MQ=255)
                                                                                                                            ttCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCtcgtc                                                  <  4:115359/119‑1 (MQ=255)
                                                                                                                            ttCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCtcgtc                                                  >  3:115359/1‑119 (MQ=255)
                                                                                                                            ttCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGc                    >  1:298075/1‑149 (MQ=255)
                                                                                                                                  ctaCCCGGCAGTATCGCCGGGTGGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGc                                 >  1:75270/3‑130 (MQ=255)
                                                                                                                                  ctaCCCGGCAGTATCGCCGGGTGGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGc                                 <  2:75270/128‑1 (MQ=255)
                                                                                                                                  ctaCCCGGCAGTATCGCCGGGTGGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGcc              >  8:65633/3‑149 (MQ=255)
                                                                                                                                  ctaCCCGGCAGTATCGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAgg                                                          <  2:273432/103‑1 (MQ=255)
                                                                                                                                  ctaCCCGGCAGTATCGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAgg                                                          >  1:273432/3‑105 (MQ=255)
                                                                                                                                  ccACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGTGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGc               <  5:368393/148‑1 (MQ=255)
                                                                                                                                  ccACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGTGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGc               >  6:368393/1‑148 (MQ=255)
                                                                                                                                    aCCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGcc              >  7:145124/1‑147 (MQ=255)
                                                                                                                                    aCCCGGCAGTATCGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGcc              >  8:310013/1‑147 (MQ=255)
                                                                                                                                      ccGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAgg          <  3:296018/149‑1 (MQ=255)
                                                                                                                                         gCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAgg          <  5:323915/146‑1 (MQ=255)
                                                                                                                                         gCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAgg          >  6:323915/1‑146 (MQ=255)
                                                                                                                                           gATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCaaa     >  6:368010/1‑149 (MQ=255)
                                                                                                                                               cTGCCGGGTAGGCCGCCGTCTGGCGCTCGTCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAacac  <  4:101085/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CCACCCATGGCCAGGAATTCGCTGCGCGAAACCGGCACGAAGCGCACCCGGTCACCGGGTTGC‑AAGAGGCTGTAACCCTCACGCTCACGGTCGAACAACCGCACCGGGGTGCGCCCGATCAGGTTCCAGCCACCCGGCGATACTGCCGGGTAGGCCGCCGTCTGGCGCTCGGCAATACCCACGCTGCCCGCCGCCACGCGCTTGCGTGGGGTACTCAGACGAG‑GGCTGGCCAGGCGCTCGTCGACCAGGCCCATGAAGCCGAAACCCGGTGCGAAGCCCAGGGCAAACAC  >  NC_002947/5195847‑5196136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑