Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,197,502 G→A 6.8% T361M (ACG→ATG)  PP_4578 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,197,5020GA 6.8% 177.3 / 2.1 61T361M (ACG→ATG) PP_4578MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (28/28);  new base A (2/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.16e-01

ATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTGACCGGCATCTGCGTGTACAACAGCACAATGACGAAAGAGCCCACGGC  >  NC_002947/5197358‑5197647
                                                                                                                                                |                                                                                                                                                 
aTGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTggg                                                                                                                                               <  8:320236/149‑1 (MQ=255)
 tGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTgggg                                                                                                                                              <  4:56346/149‑1 (MQ=255)
      tAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAg                                                                                                                                         >  4:209682/1‑149 (MQ=255)
            acCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGt                                                                                                                                   >  6:25989/1‑149 (MQ=255)
              cGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGt                                                                                                                                   >  5:386681/1‑147 (MQ=255)
              cGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCa                                                                                                                                 <  2:212543/149‑1 (MQ=255)
               gCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAg                                                                                                                                <  3:209682/149‑1 (MQ=255)
                cAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAg                                                                                                                                >  1:292587/1‑148 (MQ=255)
                    aaaCACGCAGATACCCAGGCCCAGCCTAACATTCTGGCCGAGCATGCCGATCATAAACGGAAACAGCGCGGCAATGACCTTGACGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAAct                                                                                                                           >  2:49048/1‑148 (MQ=255)
                           ccGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCAt                                                                                                                    <  2:38295/149‑1 (MQ=255)
                             gATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGc                                                                                                                  <  2:292587/149‑1 (MQ=255)
                               tACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCg                                                                                                                <  5:25989/149‑1 (MQ=255)
                                  ccAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCt                                                                                                              >  1:368474/1‑148 (MQ=255)
                                  ccAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCt                                                                                                              >  5:270158/1‑148 (MQ=255)
                                   cAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGa                                                                                                            <  6:255910/149‑1 (MQ=255)
                                           gCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATAccc                                                                                                    <  2:368474/149‑1 (MQ=255)
                                              gAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAg                                                                                                 >  7:192591/1‑149 (MQ=255)
                                                aCATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGc                                                                                               <  7:296848/149‑1 (MQ=255)
                                                        gCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCgaaga                                                                                          <  6:270158/146‑1 (MQ=255)
                                                           gAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGAGAAGAAGccc                                                                                     >  2:354998/1‑148 (MQ=255)
                                                             gCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAAc                                                                                  <  4:171727/149‑1 (MQ=255)
                                                                tgcagcacatcaACCTAAACACCCCCGCAATGACCTTGCCGATGTTGTAACCGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACggg                                                                               <  6:386681/142‑1 (MQ=255)
                                                                   cGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAgg                                                                            >  1:343675/1‑149 (MQ=255)
                                                                       catcaACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCa                                                                        >  8:318728/1‑149 (MQ=255)
                                                                         tcaACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGt                                                                                                                                   <  6:267195/88‑1 (MQ=255)
                                                                         tcaACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGt                                                                                                                                   >  5:267195/1‑88 (MQ=255)
                                                                           aaCGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCAGAATGCGCGTGGGGAACAGTTCGGTCTGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCca                                                                    <  1:49048/149‑1 (MQ=255)
                                                                             cGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCcaca                                                                  <  7:318728/149‑1 (MQ=255)
                                                                               gAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCgaagaa                                                                                         >  6:333215/1‑124 (MQ=255)
                                                                               gAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCgaagaa                                                                                         <  5:333215/124‑1 (MQ=255)
                                                                                   cAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCg                                                                                              <  6:215564/115‑1 (MQ=255)
                                                                                   cAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCg                                                                                              >  5:215564/1‑115 (MQ=255)
                                                                                   cAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCa                                                            <  2:343675/149‑1 (MQ=255)
                                                                                    aGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATAc                                                                                                      >  3:85546/1‑106 (MQ=255)
                                                                                    aGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATAc                                                                                                      <  4:85546/106‑1 (MQ=255)
                                                                                    aGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAg                                                                                                 <  8:351237/111‑1 (MQ=255)
                                                                                    aGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAg                                                                                                 >  7:351237/1‑111 (MQ=255)
                                                                                       tcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCcac                                                                   <  2:272568/137‑1 (MQ=255)
                                                                                       tcgGCAAGGTCATTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCcac                                                                   >  1:272568/2‑138 (MQ=255)
                                                                                       tcgGCAAGGTCATTGCCGATGTTGTAACAAAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGATGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGc                                                         >  8:309583/2‑148 (MQ=255)
                                                                                           cAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCg                                                    >  3:304177/1‑149 (MQ=255)
                                                                                                aCCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTGAc                                               >  7:311406/1‑149 (MQ=255)
                                                                                                 ccTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGc                                                         <  2:306916/138‑1 (MQ=255)
                                                                                                 ccTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCACCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGc                                                         >  1:306916/1‑138 (MQ=255)
                                                                                                      ccGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGaa                                                                                                                                            >  4:7960/1‑50 (MQ=255)
                                                                                                      ccGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGaa                                                                                                                                            <  3:7960/50‑1 (MQ=255)
                                                                                                          tgttgtAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTGACCGGCATCTGc                                     <  8:192591/149‑1 (MQ=255)
                                                                                                            ttgtAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTGACCGGCATCTGCgt                                   <  2:34045/149‑1 (MQ=255)
                                                                                                              gtAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTGACCGGCATCTGCgtg                                  >  3:372538/1‑148 (MQ=255)
                                                                                                               tAACAGAAGCCCTGCCCCGAACCCCGAATGCGCATGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAgg                                                                                                <  1:20196/85‑1 (MQ=255)
                                                                                                               tAACAGAAGCCCTGCCCCGAACCCCGAATGCGCATGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAgg                                                                                                >  2:20196/1‑85 (MQ=255)
                                                                                                               tAACAGAAGCCCTGCCCCGAACCCCGAATGCGCATGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAgg                                                                                                >  2:14337/1‑85 (MQ=255)
                                                                                                               tAACAGAAGCCCTGCCCCGAACCCCGAATGCGCATGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAgg                                                                                                <  1:14337/85‑1 (MQ=255)
                                                                                                                aaCAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAg                                                                             <  6:83024/103‑1 (MQ=255)
                                                                                                                aaCAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAg                                                                             >  5:83024/1‑103 (MQ=255)
                                                                                                                aaCAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTGACCGGCATCTGCGTGTa                                >  5:236780/1‑148 (MQ=255)
                                                                                                                  cAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTGACCGGCATCTGCGTGTacaa                             >  5:172354/1‑149 (MQ=255)
                                                                                                                   aGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTGACCGGCATCTGCGTGTacaac                            <  8:216905/149‑1 (MQ=255)
                                                                                                                   aGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTGACCGGCATCTGCGTGTacaa                             >  7:216905/1‑148 (MQ=255)
                                                                                                                                   accccgaaTGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTGACCGGCATCTGCGTGTACAACAGCACAATGACGa               >  4:466/1‑146 (MQ=255)
                                                                                                                                        gaaTGCGCCTGGTGAACAGTTCGGTCATAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTGACCGGCATCTGCGTGTACAACAGCACAATGACGAAAGAGccc       <  6:172354/149‑1 (MQ=255)
                                                                                                                                              gcgTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTGACCGGCATCTGCGTGTACAACAGCACAATGACGAAAGAGCCCACGGc  >  5:263464/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ATGCCGTAGGACACCGCAGAAAACACGCCGATACCCAGGCCCAGCGGAACATTCTGGCCGAGCATGCCGATCATCAACGGAAACAGCGCGGCAATGACCTTGCCGATGTTGTAACAGAAGCCCTGCCCCGAACCCCGAATGCGCGTGGGGAACAGTTCGGTCAGAAACGCCCCCATGCCGCTGAAGATACCCGAGGCGAAGAAGCCCAACGGGAGGCCCAGCCACAGCATCACGCCATCGCTGACCGGCATCTGCGTGTACAACAGCACAATGACGAAAGAGCCCACGGC  >  NC_002947/5197358‑5197647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑