Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,208,682 G→A 8.8% D9D (GAC→GAT) ‡ PP_4589 ← D‑isomer specific 2‑hydroxyacid dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,208,6820GA 8.8% 190.4 / 6.8 68D9D (GAC→GAT) ‡PP_4589D‑isomer specific 2‑hydroxyacid dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (27/35);  new base A (3/3);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.46e-01

TTGGCGCAGCAGACTGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCACCTTGGTG  >  NC_002947/5208535‑5208828
                                                                                                                                                    |                                                                                                                                                  
ttGGCGCAGCAGACTGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGt                                                                                                                                                   >  2:64677/1‑149 (MQ=255)
  ggCGCAGCAGACTGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTc                                                                                                                                                  <  1:220076/148‑1 (MQ=255)
   gcgcAGCAGACTGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCAt                                                                                                                                                >  6:127654/1‑149 (MQ=255)
     gcagcaGACTGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGc                                                                                                                                              <  6:69298/149‑1 (MQ=255)
     gcagcaGACTGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGc                                                                                                                                              >  2:229391/1‑149 (MQ=255)
                gCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCa                                                                                                                                    <  6:42107/148‑1 (MQ=255)
                 ccAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAg                                                                                                                                   >  5:28138/1‑148 (MQ=255)
                             gCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGt                                                                                                                       >  6:132409/1‑148 (MQ=255)
                                ggCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGa                                                                                                                   >  8:175391/1‑149 (MQ=255)
                                   ccAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACgg                                                                                                                >  8:110696/1‑149 (MQ=255)
                                       ccACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTAcg                                                                                                                               <  4:291852/130‑1 (MQ=255)
                                       ccACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTAcg                                                                                                                               >  3:291852/1‑130 (MQ=255)
                                       ccACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTc                                                                                                            >  3:77230/1‑149 (MQ=255)
                                         acacCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCGACTGTTCAGCACTTCCAGTTCTGGCGCGCCGCCCCGCAAACCCGCGGCGTAACTCGCATGGTAATGCTCAGCGAGCAGTCCGCGCATGTTCAGACGGGGGCg                                                                                                           >  1:84795/1‑149 (MQ=255)
                                          cacCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCT‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGtt                                                                                                         <  1:229391/149‑1 (MQ=255)
                                           acCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGACCATGCTcga                                                                                                                                          >  1:351510/1‑113 (MQ=255)
                                           acCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGACCATGCTcga                                                                                                                                          <  2:351510/115‑3 (MQ=255)
                                            ccGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTgc                                                                                                       <  6:271190/149‑1 (MQ=255)
                                             cGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTgcg                                                                                                      <  2:230526/149‑1 (MQ=255)
                                              gggCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTgcgg                                                                                                     <  1:225253/149‑1 (MQ=255)
                                              gggCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTgcgg                                                                                                     <  1:64677/149‑1 (MQ=255)
                                                  aCTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCgca                                                                                                 <  2:306052/149‑1 (MQ=255)
                                                     gCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCgcag                                                                                                >  3:161006/1‑147 (MQ=255)
                                                          gCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGctct                                                                                         >  5:6645/1‑149 (MQ=255)
                                                           ccTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCtt                                                                                        <  7:175391/149‑1 (MQ=255)
                                                            cTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTc                                                                                       <  2:369267/149‑1 (MQ=255)
                                                            cTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTc                                                                                       <  2:317695/149‑1 (MQ=255)
                                                               gCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCggg                                                                                    >  8:29418/1‑149 (MQ=255)
                                                               gCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCg                                                                                      <  4:102773/147‑1 (MQ=255)
                                                                  ggCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAg                                                                                 <  4:132993/149‑1 (MQ=255)
                                                                     cAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTg                                                                              >  8:23253/1‑149 (MQ=255)
                                                                          cGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCg                                                                          <  7:110696/148‑1 (MQ=255)
                                                                          cGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCg                                                                          <  4:161006/148‑1 (MQ=255)
                                                                           ggCAGCGTCGCCG‑CTGGGCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGCCGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGAt                                                                        <  8:205286/149‑1 (MQ=255)
                                                                           ggCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGAt                                                                        >  3:33697/1‑149 (MQ=255)
                                                                           ggCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGAt                                                                        >  3:55366/1‑149 (MQ=255)
                                                                              agagTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTa                                                                     >  6:338052/1‑149 (MQ=255)
                                                                              agagTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTa                                                                     <  7:23253/149‑1 (MQ=255)
                                                                               gagTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTAc                                                                    <  7:8380/149‑1 (MQ=255)
                                                                               gagTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTAc                                                                    <  5:132409/149‑1 (MQ=255)
                                                                                  tCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCa                                                                 <  4:55366/149‑1 (MQ=255)
                                                                                  tCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCa                                                                 >  6:310869/1‑149 (MQ=255)
                                                                                    gccg‑cTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCAt                                                                <  4:57889/148‑1 (MQ=255)
                                                                                                  aCTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGcggcgg                                                   <  4:77230/148‑1 (MQ=255)
                                                                                                     tCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATc                                               >  5:93565/1‑149 (MQ=255)
                                                                                                        aGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGc                                            <  7:29418/149‑1 (MQ=255)
                                                                                                        aGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGc                                            <  2:84795/149‑1 (MQ=255)
                                                                                                                 gcggcggcgCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGcggcgg                                                   >  8:91607/1‑133 (MQ=255)
                                                                                                                 gcggcggcgCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGcggcgg                                                   <  7:91607/133‑1 (MQ=255)
                                                                                                                 gcggcggcgCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAg                                   >  3:273444/1‑149 (MQ=255)
                                                                                                                     cggcgCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCtt                               <  4:348363/149‑1 (MQ=255)
                                                                                                                       gcgcgcAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTgg                             >  6:139874/1‑149 (MQ=255)
                                                                                                                           gcAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCttt                         >  7:340360/1‑149 (MQ=255)
                                                                                                                               aaGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAg                     <  4:273444/149‑1 (MQ=255)
                                                                                                                                aGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAgg                    <  5:127654/149‑1 (MQ=255)
                                                                                                                                 gcgcGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGt                   >  2:280096/1‑149 (MQ=255)
                                                                                                                                           tCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCtt                               <  6:9645/127‑1 (MQ=255)
                                                                                                                                           tCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCtt                               >  5:9645/1‑127 (MQ=255)
                                                                                                                                           tCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCAc         >  6:214032/1‑149 (MQ=255)
                                                                                                                                           ctgAGCATGACCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCg                       >  6:184610/3‑135 (MQ=255)
                                                                                                                                           ctgAGCATGACCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCg                       <  5:184610/133‑1 (MQ=255)
                                                                                                                                           ctgAGCATGACCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCAc         <  5:302706/147‑1 (MQ=255)
                                                                                                                                           ctgAGCATGACCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCAc         >  6:302706/3‑149 (MQ=255)
                                                                                                                                             gAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCACCt       >  7:359126/1‑149 (MQ=255)
                                                                                                                                               gCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCACCTTg     <  5:139874/149‑1 (MQ=255)
                                                                                                                                                cATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCACCTTgg    <  6:6645/149‑1 (MQ=255)
                                                                                                                                                 aTGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCACCTTgg    <  8:359126/148‑1 (MQ=255)
                                                                                                                                                  tGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGGTCTCCAGCACCTTGGTg  <  4:33697/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTGGCGCAGCAGACTGGCCAGCAAGTCCGGCTGGCCCAGCCACACCGGGCACTGCGGTGCCTGGCGGGCCAGTTCGGCAGAGTCGCCG‑CTGGTCAGCACTTCCAGTTCTGGCGCGGCGGCGCGCAAAAGCGCGGCGTATCGAGCATGGTCATGCTCAGCGATCAGTACGCGCATGGTCAGACGGGGTCGTTGCGGCGCAGCAGCTCTTCGGGCAGGTGCTCGATGTACTCATCTTCCAGCGGCGGCATCTGCAGGTGGTAGCCTTGGCTTTCGAGGTTCTCCAGCACCTTGGTG  >  NC_002947/5208535‑5208828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑