Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,212,488 C→A 7.5% intergenic (+52/+42) PP_4593 → / ← PP_4594 hypothetical protein/cystathionine gamma‑synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,212,4880CA 7.5% 296.6 / 8.0 93intergenic (+52/+42)PP_4593/PP_4594hypothetical protein/cystathionine gamma‑synthase
Reads supporting (aligned to +/- strand):  ref base C (41/45);  new base A (5/2);  total (46/47)
Fisher's exact test for biased strand distribution p-value = 2.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCC  >  NC_002947/5212340‑5212631
                                                                                                                                                     |                                                                                                                                               
cAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGAC‑AGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAcc                                                                                                                                                >  2:103570/1‑149 (MQ=255)
 aaCGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACAcc                                                                                                                                                >  2:283333/1‑149 (MQ=255)
    ggCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACcgtc                                                                                                                                             <  7:19125/149‑1 (MQ=255)
      cACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACcgtcgt                                                                                                                                           <  1:351008/149‑1 (MQ=255)
        ccAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCATGGACAAGATCTAACGGATCATTCATCCGCCCAAATCCTTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTa                                                                                                                                          <  1:108921/149‑1 (MQ=255)
        ccAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAg                                                                                                                                         <  1:86665/149‑1 (MQ=255)
        ccAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAg                                                                                                                                         <  7:157335/149‑1 (MQ=255)
        ccAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGACTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAg                                                                                                                                         >  5:360740/1‑149 (MQ=255)
          aaCGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAg                                                                                                                                         >  4:28048/1‑147 (MQ=255)
            cgacgaAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGAC‑AGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCg                                                                                                                                    <  1:103570/149‑1 (MQ=255)
              acgaAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAATGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCgg                                                                                                                                   <  5:300553/149‑1 (MQ=255)
              acgaAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCgg                                                                                                                                   <  3:135831/149‑1 (MQ=255)
               cgaAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCggg                                                                                                                                  <  3:194897/149‑1 (MQ=255)
                gaAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGt                                                                                                                                 <  3:189720/149‑1 (MQ=255)
                gaAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGt                                                                                                                                 >  3:327140/1‑149 (MQ=255)
                 aaGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGtt                                                                                                                                <  4:47758/149‑1 (MQ=255)
                  aGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGtt                                                                                                                                >  6:225367/1‑148 (MQ=255)
                      gTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTAccc                                                                                                                           <  4:327140/149‑1 (MQ=255)
                      gTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTAccc                                                                                                                           <  8:336234/149‑1 (MQ=255)
                       tCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCcg                                                                                                                          <  2:174354/149‑1 (MQ=255)
                       tCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACAcg                                                                                                                          >  5:45830/1‑149 (MQ=255)
                                     aaGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTGTACCCGCGAAAGGGCCTGCg                                                                                                            >  8:349644/1‑149 (MQ=255)
                                        gAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGcc                                                                                                         <  7:349644/149‑1 (MQ=255)
                                        gAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGcc                                                                                                         >  4:205369/1‑149 (MQ=255)
                                        gAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGcc                                                                                                         <  3:205369/149‑1 (MQ=255)
                                              ttGCTGATCAAGGACAAGATCGAACCGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGCGCCTGCGGCCCTcaca                                                                                                   >  2:308504/1‑149 (MQ=255)
                                                gCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTcacaca                                                                                                 >  7:199828/1‑149 (MQ=255)
                                                gCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTcaca                                                                                                   >  8:101805/1‑147 (MQ=255)
                                                  tGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGc                                                                                                             >  3:96440/1‑135 (MQ=255)
                                                  tGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGc                                                                                                             <  4:96440/135‑1 (MQ=255)
                                                  tGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGCAGGCCCtttcgc                                                                                                  <  6:268857/146‑6 (MQ=255)
                                                  tGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGCAGGCCCtttcgc                                                                                                  >  5:268857/1‑141 (MQ=255)
                                                    aTCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAGAGGccggtgg                                                                                                           >  1:88299/1‑130 (MQ=18)
                                                    aTCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAGAGGccggtgg                                                                                                           <  2:88299/135‑6 (MQ=18)
                                                      cAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGAc                                                                                            >  5:233313/1‑148 (MQ=255)
                                                      cAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGAc                                                                                            >  3:256479/1‑148 (MQ=255)
                                                       aaGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGAc                                                                                            >  4:88695/1‑147 (MQ=255)
                                                         ggACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAgc                                                                                        <  5:237260/149‑1 (MQ=255)
                                                          gACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAgcg                                                                                       >  8:346264/1‑149 (MQ=255)
                                                            cAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCt                                                                                     >  1:9991/1‑149 (MQ=255)
                                                            cAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCt                                                                                     >  7:206661/1‑149 (MQ=255)
                                                               gATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCttt                                                                                   >  6:358411/1‑148 (MQ=255)
                                                               gATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTc                                                                                  <  8:206661/149‑1 (MQ=255)
                                                                    aaCGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCcgc                                                                                                                         <  5:348465/105‑1 (MQ=255)
                                                                    aaCGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCcgc                                                                                                                         >  6:348465/1‑105 (MQ=255)
                                                                          tcattcatCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCgg                                                                                                           >  4:303023/1‑113 (MQ=255)
                                                                          tcattcatCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCgg                                                                                                           >  4:51681/1‑113 (MQ=255)
                                                                          tcattcatCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCgg                                                                                                           <  3:51681/113‑1 (MQ=255)
                                                                          tcattcatCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCgg                                                                                                           <  3:303023/113‑1 (MQ=255)
                                                                            attcatCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATc                                                                       >  2:114002/1‑147 (MQ=255)
                                                                            attcatCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCa                                                                     <  5:10367/149‑1 (MQ=255)
                                                                             ttcatCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAg                                                                    <  6:183548/149‑1 (MQ=255)
                                                                             ttcatCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAg                                                                    >  5:113258/1‑149 (MQ=255)
                                                                               catCCGCCCAAATCC‑TGACTGGCTTCTATTGAATCCACCGGCCTCTTCGCGGGCAAGGCCGCTCCTACACCGTCGTAGGTGCGGGTTTACCCGCGATAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAgg                                                                   <  1:308504/148‑1 (MQ=255)
                                                                                atCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTc                                                                 >  8:63415/1‑149 (MQ=255)
                                                                                   ggCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTc                                                              <  3:88695/148‑1 (MQ=255)
                                                                                   cGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTc                                                              <  1:283333/149‑1 (MQ=255)
                                                                                     cccAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGCCCAGCGCTTTCAACGCCCCCTCCAGGGCCTCGa                                                            >  2:199009/1‑149 (MQ=255)
                                                                                      ccAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGAc                                                           >  5:96277/1‑149 (MQ=255)
                                                                                       cAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGAc                                                           >  3:49545/1‑148 (MQ=255)
                                                                                        aaaTCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGAc                                                           >  6:84354/1‑147 (MQ=255)
                                                                                             c‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCTAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCa                                                    <  8:342403/149‑1 (MQ=255)
                                                                                                 aCAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                                 <  7:346264/149‑1 (MQ=255)
                                                                                                 aCAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                                 <  6:113258/149‑1 (MQ=255)
                                                                                                 aCAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACg                                                  <  6:233313/148‑1 (MQ=255)
                                                                                                   aGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGt                                                >  5:387353/1‑148 (MQ=255)
                                                                                                     gCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAAt                                             >  3:284057/1‑149 (MQ=255)
                                                                                                      cATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATc                                            <  2:9991/149‑1 (MQ=255)
                                                                                                      cATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATc                                            <  7:101805/149‑1 (MQ=255)
                                                                                                          tATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCCAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCATTCAACGCCCCAACCAGGTCCTCCACCAGCTCACGGTAATCCTc                                         >  2:343347/1‑148 (MQ=38)
                                                                                                            ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTAccc                                                                                                                           <  1:364129/64‑1 (MQ=255)
                                                                                                            ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTAccc                                                                                                                           >  2:364129/1‑64 (MQ=255)
                                                                                                            ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATc                                            >  8:250606/1‑143 (MQ=255)
                                                                                                            ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATc                                            <  7:250606/143‑1 (MQ=255)
                                                                                                            ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGa                                       <  3:28048/148‑1 (MQ=255)
                                                                                                             tGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGAt                                      <  1:199009/148‑1 (MQ=255)
                                                                                                                   cACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTAACCGCGAAAGGGCCTGCGGCCCTCACACCGCGACCAGCGCTATCAACGCCCCATCCAGGTCCTCGACCAGGACACGGTAATCCTCGATCCCTAcc                               >  6:77481/1‑149 (MQ=255)
                                                                                                                    aCCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGAAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAcc                               >  4:54870/1‑148 (MQ=255)
                                                                                                                          ctctTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGGCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAccc                        >  6:391006/1‑149 (MQ=255)
                                                                                                                           tctTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCg                       >  6:250568/1‑149 (MQ=255)
                                                                                                                            tttCGCGGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCg                       >  8:206823/2‑148 (MQ=38)
                                                                                                                             ttCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCg                       <  5:225367/147‑1 (MQ=255)
                                                                                                                               cgcgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCgca                     <  5:358411/147‑1 (MQ=255)
                                                                                                                                 cgGGTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGcagcag                 >  3:160673/1‑149 (MQ=37)
                                                                                                                                 cgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGcagcag                 <  1:343347/149‑1 (MQ=255)
                                                                                                                                  gggTAAACCCGCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGACCCCTACCGACAACCGCAGCAgg                <  2:93104/149‑1 (MQ=37)
                                                                                                                                  gggCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAgg                <  7:63415/149‑1 (MQ=255)
                                                                                                                                         cccGCTCCTACGACGGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGc         >  1:105406/1‑149 (MQ=255)
                                                                                                                                         cccGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGc         >  3:209419/1‑149 (MQ=255)
                                                                                                                                            gCTCCTACGACGGTGTAGGAGCGGGCTTGCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGTGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGa      >  8:217819/1‑149 (MQ=38)
                                                                                                                                             cTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGAt     <  4:256479/149‑1 (MQ=255)
                                                                                                                                                cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTa                                               >  1:378001/1‑104 (MQ=255)
                                                                                                                                                cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTa                                               <  2:378001/104‑1 (MQ=255)
                                                                                                                                                cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCTACAACCGCAGCATGTTCTCGCTGATcc   <  8:199828/148‑1 (MQ=255)
                                                                                                                                                cTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATccc  >  4:184911/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
CAACGGCACCAACGACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCC‑TGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCC  >  NC_002947/5212340‑5212631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑