Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,213,894 A→G 9.7% T42A (ACC→GCC)  ybaO → LRP‑like transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,213,8940AG 9.7% 106.8 / 3.0 41T42A (ACC→GCC) ybaOLRP‑like transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (17/20);  new base G (2/2);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

AATCGTCTGGCCTTGGGGTAATCTGTGAAATATTTTCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTGGGGCTCACCGTGTTCGTCCAGCTCAAGACCCAGCGCCACGACAGCGCCTGGCTGGA  >  NC_002947/5213747‑5214042
                                                                                                                                                   |                                                                                                                                                    
caTCGCCTGGCCTTGTGGTAATCTGTGCAATATTTTCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTccac                                                                                                                                                     <  6:324978/148‑1 (MQ=255)
         gCCTTGGGGTAATCTGTGAAATATTTTCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTg                                                                                                                                            >  1:156686/1‑149 (MQ=255)
           cTTGGGGTAATCTGTGAAATATTTTCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCt                                                                                                                                          >  8:308528/1‑149 (MQ=255)
                    aTCTGTGAAATATTTTCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCgg                                                                                                                                  <  7:260083/148‑1 (MQ=255)
                    aTCTGTGAAATATTTTCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCgg                                                                                                                                  <  6:139670/148‑1 (MQ=255)
                          gAAATATTTTCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGc                                                                                                                                    >  4:182346/1‑140 (MQ=255)
                          gAAATATTTTCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGc                                                                                                                                    <  3:182346/140‑1 (MQ=255)
                           aaaTATTTTCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAgcg                                                                                                                          >  3:175354/1‑149 (MQ=255)
                           aaaTATTTTCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAgc                                                                                                                           <  6:238043/148‑1 (MQ=255)
                              tatTTTCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCt                                                                                                                       <  2:372601/149‑1 (MQ=255)
                              tatTTTCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCt                                                                                                                       <  8:112380/149‑1 (MQ=255)
                                ttttCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACTGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGGCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTg                                                                                                                      <  7:173315/148‑1 (MQ=255)
                                  ttCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTgga                                                                                                                    <  3:339939/148‑1 (MQ=255)
                                  ttCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTgga                                                                                                                    <  7:145688/148‑1 (MQ=255)
                                           ggCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGc                                                                                                          <  7:308528/149‑1 (MQ=255)
                                              cAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTAc                                                                                                       <  2:156686/149‑1 (MQ=255)
                                                           ggACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCcgc                                                                                           >  4:3971/1‑148 (MQ=255)
                                                            gACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCgg                                                                                                                                  <  2:228955/108‑1 (MQ=255)
                                                            gACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCgg                                                                                                                                  >  1:228955/1‑108 (MQ=255)
                                                               aGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGc                                                                                      >  1:8381/1‑149 (MQ=255)
                                                                     gACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCctgct                                                                                <  3:3971/149‑1 (MQ=255)
                                                                      aCCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCctgctg                                                                               >  3:82301/1‑149 (MQ=255)
                                                                           cacaTTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGAccc                                                                          <  2:8381/149‑1 (MQ=255)
                                                                            acaTTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGAcccc                                                                         >  2:147358/1‑149 (MQ=255)
                                                                                     cccTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTg                                                                <  5:74522/149‑1 (MQ=255)
                                                                                           tACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCGCCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTg                                                          >  7:361065/1‑149 (MQ=255)
                                                                                           tACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCGCCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTg                                                          >  7:19757/1‑149 (MQ=255)
                                                                                              aGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTgggg                                                       <  4:175354/149‑1 (MQ=255)
                                                                                                        cTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTGGGGCTCa                                                   <  5:153800/143‑1 (MQ=255)
                                                                                                        cTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTGGGGCTCa                                                   >  6:153800/1‑143 (MQ=255)
                                                                                                             tctcGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTGGGGCTCACCGTGTTCGTc                                        >  7:313307/1‑149 (MQ=255)
                                                                                                             tctcGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTGGGGCTCACCGTGTTCGTc                                        >  3:44271/1‑149 (MQ=255)
                                                                                                                 gCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCGCCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTGGGGCTCACCGTGTTCGTcc                                       <  8:19757/146‑1 (MQ=255)
                                                                                                                 gCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCGCCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTGGGGCTCACCGTGTTCGTcc                                       <  8:361065/146‑1 (MQ=255)
                                                                                                                 gCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTGGGGCTCACCGTGTTCGTc                                        <  8:313307/145‑1 (MQ=255)
                                                                                                                             ggCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTGGGGCTCACCGTGTTCGTCCAGCTCAAGACCCAgc                        >  4:256690/1‑149 (MQ=255)
                                                                                                                                       gTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGTCGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGTACGCCTGGGGTTGGGGCTCACCGTGTTCGTCCAg                                     <  5:29944/126‑1 (MQ=255)
                                                                                                                                       gTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCATGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTGGGGCTCACCGTCTTCGTCAAg                                     >  6:29944/1‑126 (MQ=255)
                                                                                                                                          aaCCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGATGGGGCTCACCGTGTTCGTCCAGCTCAAGACCCAGCGCCACGACAgcgc           >  1:13004/1‑149 (MQ=255)
                                                                                                                                             cTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTGGGGCTCACCGTGTTCGTCCAGCTCAAGACCCAGCGCAACGACAGATCctg        >  6:283911/1‑149 (MQ=255)
                                                                                                                                              tGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTggg                                                              <  2:115797/94‑1 (MQ=255)
                                                                                                                                              tGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTggg                                                              >  1:115797/1‑94 (MQ=255)
                                                                                                                                                   accacACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTGGGGCTCACCGTGTTCGTCCAGCTCAAGACCCAGCGCCACGACAGCGCctggctg    >  3:137256/1‑147 (MQ=255)
                                                                                                                                                   accacACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTGGGGCTCACCGTGTTCGTCCAGCTCAAGACCCAGCGCCACGACAGCGCCTGGCTGGa  <  3:256690/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AATCGTCTGGCCTTGGGGTAATCTGTGAAATATTTTCCTGCACGGCCAGCGCGCGATATGGACAGTTTCGACCAGCACATTCTTACCCTGCTACAGCGTGATGCCTCAATCTCGCTGAAGGACCTGGCCGAGGCCGTCAACCTGTCCACCACACCGTGCTGGAAGCGGGTCAAGCGCCTGGAGGAAGACGGCTACATCCTCGGCCGCGTCGCCCTGCTGGACCCCGAACGCCTGGGGTTGGGGCTCACCGTGTTCGTCCAGCTCAAGACCCAGCGCCACGACAGCGCCTGGCTGGA  >  NC_002947/5213747‑5214042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑