Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,246,070 A→C 6.3% L864R (CTC→CGC)  PP_4623 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,246,0700AC 6.3% 199.3 / 3.6 63L864R (CTC→CGC) PP_4623hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/31);  new base C (2/2);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

CGGCATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACGGATAAAGGTTGCACGCCGCGT  >  NC_002947/5245924‑5246211
                                                                                                                                                  |                                                                                                                                             
cGGCATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAgg                                                                                                                                             >  3:332237/1‑149 (MQ=255)
       cAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCg                                                                                                                                        >  8:43077/1‑147 (MQ=255)
         gCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGc                                                                                                                                    >  5:79612/1‑149 (MQ=255)
           accaccCGAATGCCTTCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAAGGGCTCGCTGTCTTGGAAATAACCTGCCTAGTCGTGCTCAGGCAAAGTCCTTGCCAGCTCGCTTAGGGTCCGCAGCGt                                                                                                                                  <  2:111279/149‑1 (MQ=255)
            ccaccCCAATGCCATCGCCGGCTCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTg                                                                                                                                 <  6:360215/149‑1 (MQ=255)
            ccaccCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGCGGACCCTCAGCgag                                                                                                                                 >  4:91945/1‑147 (MQ=255)
             caccCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGCGGACCCTCAGCgagc                                                                                                                                <  3:91945/149‑4 (MQ=255)
             caccCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTgg                                                                                                                                >  6:19726/1‑149 (MQ=255)
              accCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTggg                                                                                                                               <  5:391033/149‑1 (MQ=255)
                        cATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTc                                                                                                                      >  5:40653/1‑148 (MQ=255)
                           cGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGCGGACCCTCAGCgag                                                                                                                                 >  6:51375/1‑132 (MQ=255)
                           cGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGCGGACCCTCAGCgag                                                                                                                                 <  5:51375/134‑3 (MQ=255)
                             ccGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTc                                                                                                                <  4:332237/149‑1 (MQ=255)
                                gcgcgcAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTgg                                                                                                             <  6:79612/149‑1 (MQ=255)
                                 cgcgcAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGc                                                                                                            <  7:43077/149‑1 (MQ=255)
                                  gcgcAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTggg                                                                                                                               >  6:322608/1‑129 (MQ=255)
                                  gcgcAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTggg                                                                                                                               <  5:322608/129‑1 (MQ=255)
                                      aGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATa                                                                                                       <  2:138601/149‑1 (MQ=255)
                                       gTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAg                                                                                                      <  6:245965/149‑1 (MQ=255)
                                        ttGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATTACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGa                                                                                                     <  5:366791/149‑1 (MQ=255)
                                           ctctTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTgggg                                                                                                                              >  1:179841/1‑121 (MQ=255)
                                           ctctTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTgggg                                                                                                                              <  2:179841/121‑1 (MQ=255)
                                              ttCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCaa                                                                                                 <  5:19726/147‑1 (MQ=255)
                                              ttCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAg                                                                                                >  8:186627/1‑148 (MQ=255)
                                                cGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAg                                                                                                <  7:186627/146‑1 (MQ=255)
                                                cGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGc                                                                                             >  4:306323/1‑149 (MQ=255)
                                                   tCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTc                                                                                           >  3:48390/1‑148 (MQ=255)
                                                    cAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTc                                                                                           >  5:160426/1‑147 (MQ=255)
                                                         cGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTcg                                                                                    <  4:48390/149‑1 (MQ=255)
                                                          gTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCt                                                                                                               <  8:64092/121‑1 (MQ=255)
                                                          gTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCt                                                                                                               >  7:64092/1‑121 (MQ=255)
                                                          gTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCa                                                                                                           >  4:44855/1‑125 (MQ=255)
                                                          gTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCa                                                                                                           <  3:44855/125‑1 (MQ=255)
                                                               aGCGCGACTGGCTCGATAACGTCACGCGGTCTAGGAAATAACCTGCCAAGTCGTGCTCACGCCAAGACCTTGCCAGCTCGCTGAGGGTCCGCAGCCTCGGGCATTGCTCGATTTCTGGCAGATAGAACAAGGTCTCAAACTCGCGGTCa                                                                              >  4:115174/1‑149 (MQ=255)
                                                                gcgcGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCAc                                                                             <  6:40653/149‑1 (MQ=255)
                                                                             gATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTca                                                                 <  3:306323/148‑1 (MQ=255)
                                                                               tAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTc                                                                                     <  8:101157/126‑1 (MQ=255)
                                                                               tAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTc                                                                                     >  7:101157/1‑126 (MQ=255)
                                                                                  cGGGACGCGGTCTTGGCAATAACCTGCCATGTCGTGCTCAGGCCAAGTCCTTGCCAGCGCGCTGAGGGTCCGCAGCGTGGGGCGTTGCTGGATTTCTGGGTGATAGACCAAGGGCTCAAGCTCGCGGTCACGTGCATGCTTCAGCAGGt                                                           <  5:224555/149‑1 (MQ=255)
                                                                                  cGGCTCGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCTGTCACGAGCATGCTTCAGCAGGt                                                           <  3:115174/149‑1 (MQ=255)
                                                                                  cGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACTAGCATGCTTCAGCAGGt                                                           <  5:143937/149‑1 (MQ=255)
                                                                                    gCACGCGGTCTTGGCAATAACCTGCCATGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCtt                                                                   <  5:384894/139‑1 (MQ=255)
                                                                                    gCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCtt                                                                   >  6:384894/1‑139 (MQ=255)
                                                                                       cgcgGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCtt                                                                   <  8:110323/136‑1 (MQ=255)
                                                                                       cgcgGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCtt                                                                   >  7:110323/1‑136 (MQ=255)
                                                                                                  cAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACggg                                           <  6:251296/149‑1 (MQ=255)
                                                                                                   aaTAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACgggg                                          <  6:160426/149‑1 (MQ=255)
                                                                                                          tGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGt                                   >  1:137671/1‑149 (MQ=255)
                                                                                                          tGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGt                                   >  7:110964/1‑149 (MQ=255)
                                                                                                                        cACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGAGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACGGa                     >  7:332477/1‑149 (MQ=255)
                                                                                                                         aCGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACGGa                     >  7:312618/1‑148 (MQ=255)
                                                                                                                                 tCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAAcc                       >  6:58587/1‑137 (MQ=255)
                                                                                                                                 tCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACg                       <  5:58587/138‑1 (MQ=255)
                                                                                                                                    ttGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAg                                                                                        <  4:272145/70‑1 (MQ=255)
                                                                                                                                    ttGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAg                                                                                        >  3:272145/1‑70 (MQ=255)
                                                                                                                                    ttGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAg                                                                          >  6:247543/1‑84 (MQ=255)
                                                                                                                                    ttGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAg                                                                          <  5:247543/84‑1 (MQ=255)
                                                                                                                                       ccAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACGGATAAAGGTTGCAcgcc      >  1:347257/1‑149 (MQ=255)
                                                                                                                                        cAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACgggg                                          >  2:359322/1‑112 (MQ=255)
                                                                                                                                        cAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACgggg                                          <  1:359322/112‑1 (MQ=255)
                                                                                                                                           cTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCGAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACGGAt                    <  7:196430/131‑1 (MQ=255)
                                                                                                                                           cTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACGGATAAAGGTTGCACGCCGCGt  >  3:220601/1‑149 (MQ=255)
                                                                                                                                           cTCGCTGAGGGTCCGCAGCGTGGGGCCTAGCTCGATTTCTGGCAGATAGACCAAGGGCTCGAGCTCGCGGTCACGAGCATGCTTCACCAGCTAGGCACCTTGAACGGGGCAATCGTCCATCGACAACGGaa                    >  8:196430/1‑130 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
CGGCATCCAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACGGATAAAGGTTGCACGCCGCGT  >  NC_002947/5245924‑5246211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑