Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,246,074	T→C	6.4%	T863A (ACC→GCC) ‡	PP_4623 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,246,074	0	T	C	6.4%	186.0 / 2.4	62	T863A (ACC→GCC) ‡	PP_4623	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/31);  new base C (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

CAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACGGATAAAGGTTGCACGCCGCGT  >  NC_002947/5245931‑5246211                                                                                                                                                |                                                                                                                                          cAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCg                                                                                                                                        >  8:43077/1‑147 (MQ=255)   gCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGc                                                                                                                                    >  5:79612/1‑149 (MQ=255)     accaccCGAATGCCTTCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAAGGGCTCGCTGTCTTGGAAATAACCTGCCTAGTCGTGCTCAGGCAAAGTCCTTGCCAGCTCGCTTAGGGTCCGCAGCGt                                                                                                                                  <  2:111279/149‑1 (MQ=255)      ccaccCCAATGCCATCGCCGGCTCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTg                                                                                                                                 <  6:360215/149‑1 (MQ=255)      ccaccCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGCGGACCCTCAGCgag                                                                                                                                 >  4:91945/1‑147 (MQ=255)       caccCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGCGGACCCTCAGCgagc                                                                                                                                <  3:91945/149‑4 (MQ=255)       caccCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTgg                                                                                                                                >  6:19726/1‑149 (MQ=255)        accCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTggg                                                                                                                               <  5:391033/149‑1 (MQ=255)                  cATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTc                                                                                                                      >  5:40653/1‑148 (MQ=255)                     cGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGCGGACCCTCAGCgag                                                                                                                                 >  6:51375/1‑132 (MQ=255)                     cGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGCGGACCCTCAGCgag                                                                                                                                 <  5:51375/134‑3 (MQ=255)                       ccGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTc                                                                                                                <  4:332237/149‑1 (MQ=255)                          gcgcgcAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTgg                                                                                                             <  6:79612/149‑1 (MQ=255)                           cgcgcAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGc                                                                                                            <  7:43077/149‑1 (MQ=255)                            gcgcAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTggg                                                                                                                               >  6:322608/1‑129 (MQ=255)                            gcgcAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTggg                                                                                                                               <  5:322608/129‑1 (MQ=255)                                aGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATa                                                                                                       <  2:138601/149‑1 (MQ=255)                                 gTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAg                                                                                                      <  6:245965/149‑1 (MQ=255)                                  ttGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATTACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGa                                                                                                     <  5:366791/149‑1 (MQ=255)                                     ctctTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTgggg                                                                                                                              >  1:179841/1‑121 (MQ=255)                                     ctctTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTgggg                                                                                                                              <  2:179841/121‑1 (MQ=255)                                        ttCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCaa                                                                                                 <  5:19726/147‑1 (MQ=255)                                        ttCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAg                                                                                                >  8:186627/1‑148 (MQ=255)                                          cGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAg                                                                                                <  7:186627/146‑1 (MQ=255)                                          cGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGc                                                                                             >  4:306323/1‑149 (MQ=255)                                             tCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTc                                                                                           >  3:48390/1‑148 (MQ=255)                                              cAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTc                                                                                           >  5:160426/1‑147 (MQ=255)                                                   cGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTcg                                                                                    <  4:48390/149‑1 (MQ=255)                                                    gTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCt                                                                                                               <  8:64092/121‑1 (MQ=255)                                                    gTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCt                                                                                                               >  7:64092/1‑121 (MQ=255)                                                    gTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCa                                                                                                           >  4:44855/1‑125 (MQ=255)                                                    gTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCa                                                                                                           <  3:44855/125‑1 (MQ=255)                                                         aGCGCGACTGGCTCGATAACGTCACGCGGTCTAGGAAATAACCTGCCAAGTCGTGCTCACGCCAAGACCTTGCCAGCTCGCTGAGGGTCCGCAGCCTCGGGCATTGCTCGATTTCTGGCAGATAGAACAAGGTCTCAAACTCGCGGTCa                                                                              >  4:115174/1‑149 (MQ=255)                                                          gcgcGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCAc                                                                             <  6:40653/149‑1 (MQ=255)                                                                       gATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTca                                                                 <  3:306323/148‑1 (MQ=255)                                                                         tAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTc                                                                                     <  8:101157/126‑1 (MQ=255)                                                                         tAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTc                                                                                     >  7:101157/1‑126 (MQ=255)                                                                            cGGGACGCGGTCTTGGCAATAACCTGCCATGTCGTGCTCAGGCCAAGTCCTTGCCAGCGCGCTGAGGGTCCGCAGCGTGGGGCGTTGCTGGATTTCTGGGTGATAGACCAAGGGCTCAAGCTCGCGGTCACGTGCATGCTTCAGCAGGt                                                           <  5:224555/149‑1 (MQ=255)                                                                            cGGCTCGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCTGTCACGAGCATGCTTCAGCAGGt                                                           <  3:115174/149‑1 (MQ=255)                                                                            cGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACTAGCATGCTTCAGCAGGt                                                           <  5:143937/149‑1 (MQ=255)                                                                              gCACGCGGTCTTGGCAATAACCTGCCATGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCtt                                                                   <  5:384894/139‑1 (MQ=255)                                                                              gCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCtt                                                                   >  6:384894/1‑139 (MQ=255)                                                                                 cgcgGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCtt                                                                   <  8:110323/136‑1 (MQ=255)                                                                                 cgcgGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCtt                                                                   >  7:110323/1‑136 (MQ=255)                                                                                            cAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACggg                                           <  6:251296/149‑1 (MQ=255)                                                                                             aaTAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACgggg                                          <  6:160426/149‑1 (MQ=255)                                                                                                    tGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGt                                   >  1:137671/1‑149 (MQ=255)                                                                                                    tGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGt                                   >  7:110964/1‑149 (MQ=255)                                                                                                                  cACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGAGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACGGa                     >  7:332477/1‑149 (MQ=255)                                                                                                                   aCGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACGGa                     >  7:312618/1‑148 (MQ=255)                                                                                                                           tCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAAcc                       >  6:58587/1‑137 (MQ=255)                                                                                                                           tCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACg                       <  5:58587/138‑1 (MQ=255)                                                                                                                              ttGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAg                                                                                        <  4:272145/70‑1 (MQ=255)                                                                                                                              ttGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAg                                                                                        >  3:272145/1‑70 (MQ=255)                                                                                                                              ttGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAg                                                                          >  6:247543/1‑84 (MQ=255)                                                                                                                              ttGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAg                                                                          <  5:247543/84‑1 (MQ=255)                                                                                                                                 ccAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACGGATAAAGGTTGCAcgcc      >  1:347257/1‑149 (MQ=255)                                                                                                                                  cAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACgggg                                          >  2:359322/1‑112 (MQ=255)                                                                                                                                  cAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACgggg                                          <  1:359322/112‑1 (MQ=255)                                                                                                                                     cTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCGAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACGGAt                    <  7:196430/131‑1 (MQ=255)                                                                                                                                     cTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACGGATAAAGGTTGCACGCCGCGt  >  3:220601/1‑149 (MQ=255)                                                                                                                                     cTCGCTGAGGGTCCGCAGCGTGGGGCCTAGCTCGATTTCTGGCAGATAGACCAAGGGCTCGAGCTCGCGGTCACGAGCATGCTTCACCAGCTAGGCACCTTGAACGGGGCAATCGTCCATCGACAACGGaa                    >  8:196430/1‑130 (MQ=255)                                                                                                                                                |                                                                                                                                          CAGCACCACCCCAATGCCATCGCCGGCGCGCAGTTGCTCTTCGATCAATGCGTTCAAGCGCGACTGGCTCGATAACGGCACGCGGTCTTGGCAATAACCTGCCAAGTCGTGCTCACGCCAAGTCCTTGCCAGCTCGCTGAGGGTCCGCAGCGTGGGGCCTTGCTCGATTTCTGGCAGATAGACCAAGGGCTCAAGCTCGCGGTCACGAGCATGCTTCAGCAGGTAGGCACCTTGAACGGGGCAATCGTCCATCCACAACGGATAAAGGTTGCACGCCGCGT  >  NC_002947/5245931‑5246211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑