Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,257,689 A→C 7.9% V38G (GTG→GGG)  PP_4634 ← alpha/beta family hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,257,6890AC 7.9% 194.5 / 6.1 63V38G (GTG→GGG) PP_4634alpha/beta family hydrolase
Reads supporting (aligned to +/- strand):  ref base A (26/32);  new base C (3/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GGAGGCCTGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCA  >  NC_002947/5257541‑5257836
                                                                                                                                                    |                                                                                                                                                   
ggaggCCTGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCa                                                                                                                                                     >  5:37253/1‑149 (MQ=255)
 gaggCCTGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGAAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCAc                                                                                                                                                    <  4:111694/149‑1 (MQ=255)
       tGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCgg                                                                                                                                              >  1:83557/1‑149 (MQ=255)
        ggAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCggg                                                                                                                                             >  7:1425/1‑149 (MQ=255)
         gAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCggg                                                                                                                                             <  7:158304/148‑1 (MQ=255)
         gAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGgc                                                                                                                                            >  2:81308/1‑149 (MQ=255)
            aCCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCt                                                                                                                                         <  3:111482/149‑1 (MQ=255)
             ccACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCggg                                                                                                                                             <  5:317132/144‑1 (MQ=255)
             ccACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCggg                                                                                                                                             >  6:317132/1‑144 (MQ=255)
                   aGCGAAGCCGGCCGGGATTCGCCGATGCCACGGCAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGAt                                                                                                                                   >  5:69902/1‑148 (MQ=255)
                    gCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCGGGGGATCa                                                                                                                                 <  3:339406/149‑1 (MQ=255)
                          ccggccggGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTgg                                                                                                                           <  2:219738/149‑1 (MQ=255)
                             gccggGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCg                                                                                                                        >  2:217953/1‑149 (MQ=255)
                              ccggGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAACGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGt                                                                                                                       >  1:250086/1‑149 (MQ=255)
                                gggATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTgg                                                                                                                     <  1:81308/149‑1 (MQ=255)
                                 ggATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGgc                                                                                                                    <  2:30903/149‑1 (MQ=255)
                                       gCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCcag                                                                                                               <  1:217953/148‑1 (MQ=255)
                                       gCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCcag                                                                                                               <  1:368791/148‑1 (MQ=255)
                                        ccGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCcagca                                                                                                             <  2:83557/149‑1 (MQ=255)
                                        ccGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCcag                                                                                                               >  3:24870/1‑147 (MQ=255)
                                         cGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCcagcag                                                                                                            <  6:37253/149‑1 (MQ=255)
                                           aTGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGaa                                                                                                          <  4:33171/149‑1 (MQ=255)
                                             gCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAgc                                                                                                        >  1:69020/1‑149 (MQ=255)
                                               cACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAgccg                                                                                                      >  3:339079/1‑149 (MQ=255)
                                                      gTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAgg                                                                                                <  3:202563/148‑1 (MQ=255)
                                                             gTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATcc                                                                                        >  5:293977/1‑149 (MQ=255)
                                                             gTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATcc                                                                                        >  6:127541/1‑149 (MQ=255)
                                                               cAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGt                                                                                      <  7:203638/149‑1 (MQ=255)
                                                                 gCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCg                                                                                    >  6:351619/1‑149 (MQ=255)
                                                                  cacaTCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATTATCACAAGCGGGCTCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCAGCCGAGGCTGTATACGTCgg                                                                                   >  6:214212/1‑149 (MQ=255)
                                                                    caTCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGcc                                                                                 >  7:28327/1‑149 (MQ=255)
                                                                     aTCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCcg                                                                                >  8:30152/1‑149 (MQ=255)
                                                                      tCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCt                                                                                              >  8:268463/1‑134 (MQ=255)
                                                                      tCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCt                                                                                              <  7:268463/134‑1 (MQ=255)
                                                                         cAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCggg                                                                            <  5:351619/149‑1 (MQ=255)
                                                                            ccGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTg                                                                         >  5:359886/1‑149 (MQ=255)
                                                                             cGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTgg                                                                        <  6:293977/149‑1 (MQ=255)
                                                                              gTGGGCGAACAGGTAGTCTGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGACAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGc                                                                       <  5:214212/149‑1 (MQ=255)
                                                                                     aaCAGGTAGTCGGCAAAGCGGGCGTAATATCGGCAGCGTACCGAAGTGGCAGCGGTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATgt                                                                <  2:100870/149‑2 (MQ=255)
                                                                                      aCAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATggg                                                               <  8:1425/149‑1 (MQ=255)
                                                                                      aCAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATggg                                                               <  5:127541/149‑1 (MQ=255)
                                                                                          gTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGaa                                                                                                          <  1:41386/102‑1 (MQ=255)
                                                                                          gTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGaa                                                                                                          >  2:41386/1‑102 (MQ=255)
                                                                                               cGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAAt                                                                  >  7:307630/1‑137 (MQ=255)
                                                                                               cGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAAt                                                                  <  8:307630/137‑1 (MQ=255)
                                                                                                 gCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTg                                                    >  8:357225/1‑149 (MQ=255)
                                                                                                  cAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTgg                                                   <  2:69020/149‑1 (MQ=255)
                                                                                                   aaaGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGa                                                  <  1:286918/149‑1 (MQ=255)
                                                                                                    aaGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTa                                                                                           >  6:122479/1‑107 (MQ=255)
                                                                                                    aaGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTa                                                                                           <  5:122479/107‑1 (MQ=255)
                                                                                                        gggCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAgg                                             <  6:69902/149‑1 (MQ=255)
                                                                                                             tAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGCGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACt                                        <  1:49602/149‑1 (MQ=255)
                                                                                                                  gCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATgg                                   <  2:250086/149‑1 (MQ=255)
                                                                                                                      cAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCa                               >  1:67337/1‑149 (MQ=255)
                                                                                                                                           ttGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCa           >  7:145463/1‑148 (MQ=255)
                                                                                                                                           ttGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCa           >  2:342397/1‑148 (MQ=255)
                                                                                                                                           aacctgatCCCCAGCGCCCGCTGGTGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATg                                                                 <  7:335616/90‑1 (MQ=37)
                                                                                                                                           aacctgatCCCCAGCGCCCGCTGGTGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATg                                                                 >  8:335616/5‑94 (MQ=37)
                                                                                                                                             cctgatCCCCAGCGCCCGCTGGTGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttg                     >  2:354061/3‑136 (MQ=255)
                                                                                                                                             cctgatCCCCAGCGCCCGCTGGTGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCtgttg                     <  1:354061/134‑1 (MQ=255)
                                                                                                                                              atgatCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAAc       >  5:231555/1‑149 (MQ=255)
                                                                                                                                              atgatCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAAc       <  4:339079/149‑1 (MQ=255)
                                                                                                                                               tgatCCCCAGCGCCCGCTGGTGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAAc       >  7:310969/1‑148 (MQ=255)
                                                                                                                                                 atCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAg    <  6:231555/149‑1 (MQ=255)
                                                                                                                                                    aCCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCa  >  1:90543/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGAGGCCTGGAAACCACGCAGCGAAGCCGGCCGGGATTCGCCGATGCCACGGTAGTCGTAGGTCAGCACATCGCAGCCGTGGGCGAACAGGTAGTCGGCAAAGCGGGCGTAATAGCGGCAGCGTACCGAAGTGGCAGCGTTGATGATCACCAGCGGGCGCTGGGGATCAGGTTGGGCGTGGCGCCAGCAGAAGCCGCCGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCA  >  NC_002947/5257541‑5257836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑