Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 446,384 C→G 5.2% A357G (GCC→GGC)  PP_0368 → acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947446,3840CG 5.2% 248.6 / 3.8 77A357G (GCC→GGC) PP_0368acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (35/38);  new base G (2/2);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

GAGGTGGCGTTCCAGGGTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGCCAAGGCATTCATGACCGAAGTCGGTTTC  >  NC_002947/446236‑446532
                                                                                                                                                     |                                                                                                                                                    
gAGGTGGCGTTCCAGGGTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGc                                                                                                                                                      <  8:255047/149‑1 (MQ=255)
gAGGTGGCGTTCCAGGGTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGc                                                                                                                                                      <  1:233069/149‑1 (MQ=255)
gAGGTGGCGTTCCAGGGTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGc                                                                                                                                                      >  2:109474/1‑149 (MQ=255)
    tGGCGTTCCAGGGTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGaa                                                                                                                                                  <  2:109819/149‑1 (MQ=255)
     ggCGTTCCAGGCTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTCACCCGCTCATCGTCCACCC‑GGACGTCCGTCGCATGCTGCTGACCATGAAGGCCTTCGcagcag                                                                                                                                                 >  6:376992/1‑144 (MQ=37)
      gCGTTCCAGGGTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAgg                                                                                                                                                >  6:29317/1‑149 (MQ=255)
      gCGTTCCAGGGTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAg                                                                                                                                                 <  1:266962/148‑1 (MQ=255)
       cGTTCCAGGGTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGaa                                                                                                                                                  >  2:158519/1‑146 (MQ=255)
       cGTTCCAGGGTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGaa                                                                                                                                                  <  1:158519/146‑1 (MQ=255)
        gTTCCAGGGTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGc                                                                                                                                                      >  5:79663/1‑141 (MQ=255)
        gTTCCAGGGTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGc                                                                                                                                                      <  6:79663/141‑1 (MQ=255)
            cAGGTTGGTCTGAAGTACGCCAGTGAGCTCCTGCAGATTCTTTCCCTGACCGTCCCGAAAGCACCGGACAAGGTCGCTGACCCGATAATCGTCCACCC‑GGACGTGCTGCTCATGCTGCTGACCATGAAGTCATTCGGCGAAGGccttca                                                                                                                                          <  2:260032/149‑6 (MQ=25)
            cAGGGTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAAcc                                                                                                                                           <  4:92759/148‑1 (MQ=255)
             aGGGTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACcgc                                                                                                                                         >  4:143183/1‑149 (MQ=255)
                    ccTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATgg                                                                                                                                  >  1:377820/1‑149 (MQ=255)
                    ccTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATgg                                                                                                                                  >  8:132104/1‑149 (MQ=255)
                     cTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGt                                                                                                                                 >  1:56538/1‑149 (MQ=255)
                      tGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCGTGACGGGCCCGAAAGCACCGGACATGGCCGCTGACCCGTTCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCTTCCGCGCGATGGtt                                                                                                                                <  5:376992/149‑1 (MQ=37)
                                  gTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACtt                                                                                                                           <  5:335259/142‑1 (MQ=255)
                                  gTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACtt                                                                                                                           >  6:335259/1‑142 (MQ=255)
                                   tGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCa                                                                                                                   <  7:132104/149‑1 (MQ=255)
                                     aGCGCCTGCAGATGCGTTCCCTGACAGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAg                                                                                                                 <  5:29317/149‑1 (MQ=255)
                                      gcgcCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGc                                                                                                                <  5:292682/149‑1 (MQ=255)
                                         ccTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAgg                                                                                                             >  3:371777/1‑149 (MQ=255)
                                          cTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGt                                                                                                            >  5:188969/1‑149 (MQ=255)
                                          cTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGt                                                                                                            <  6:88770/149‑1 (MQ=255)
                                          cTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGt                                                                                                            >  3:365199/1‑149 (MQ=255)
                                             cAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGa                                                                                                         >  3:267166/1‑149 (MQ=255)
                                              aGATTCGTTCCCTGACCGGCCCGAAAGCACCGGACATTGCCGCTGACCCGATCATCGTCCACAC‑GGACGTGCGTCGCATGCTGATGACCATGAAGGCCTTCGCCGAATGCAAAAGCTCGATGTTTTACTTCACCTCAAAGCTGGTGGAc                                                                                                        <  8:24036/149‑1 (MQ=25)
                                               gATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCA‑GGACGTGCGACGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCAACGCCAAGCAGGTGGAc                                                                                                        >  7:143939/1‑148 (MQ=255)
                                                   cGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGt                                                                                                   <  7:211737/149‑1 (MQ=255)
                                                    cttCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGACCACCC‑TGACGTGCGTCGCATGCTGCTGACCATAAAGGCCTTCGCCGAAGCCAACCCCGCGATGCTTTACATCACACCCAAGCAGGTCCACATAGt                                                                                                   >  2:70797/2‑148 (MQ=255)
                                                     ttCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCa                                                                                                 <  6:372046/149‑1 (MQ=255)
                                                       cccTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCaa                                                                                                <  1:75152/148‑1 (MQ=255)
                                                       cccTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCaa                                                                                                <  3:143183/148‑1 (MQ=255)
                                                        ccTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAg                                                                                               <  4:267166/148‑1 (MQ=255)
                                                         cTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTa                                                                                             <  8:162547/149‑1 (MQ=255)
                                                            aCCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAg                                                                                          <  6:188969/149‑1 (MQ=255)
                                                                  ccGAAAGCACCGGACAAGGCCGATGACCCGATCATCGTCCACCCGGGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTg                                                                                                           >  1:79416/1‑127 (MQ=255)
                                                                  ccGAAAGCACCGGACAAGGCCGATGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTg                                                                                                           <  2:79416/126‑1 (MQ=255)
                                                                     aaaGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACga                                                                                 <  1:109474/149‑1 (MQ=255)
                                                                      aaGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACgaa                                                                                <  4:365199/149‑1 (MQ=255)
                                                                      aaGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACg                                                                                  >  8:90100/1‑147 (MQ=255)
                                                                         cACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACgaagaa                                                                             <  4:371777/149‑1 (MQ=255)
                                                                           ccGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAagaaga                                                                           <  1:70797/149‑1 (MQ=255)
                                                                           ccGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAagaaga                                                                           <  2:377820/149‑1 (MQ=255)
                                                                              gACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAgcg                                                                        <  2:56538/149‑1 (MQ=255)
                                                                                 aaGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAgcgc                                                                       >  3:368510/1‑147 (MQ=255)
                                                                                   ggCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCaag                                                                    >  3:60486/1‑148 (MQ=255)
                                                                                    gCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACgaag                                                                               >  4:169535/1‑136 (MQ=255)
                                                                                    gCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACgaag                                                                               <  3:169535/136‑1 (MQ=255)
                                                                                       gCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGc                                                               >  6:122352/1‑149 (MQ=255)
                                                                                         tGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTg                                                             >  8:121396/1‑149 (MQ=255)
                                                                                           aCCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGAc                                                           >  8:128325/1‑149 (MQ=255)
                                                                                             ccGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCaa                                                                                                >  4:323428/1‑110 (MQ=255)
                                                                                             ccGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCaa                                                                                                <  3:323428/110‑1 (MQ=255)
                                                                                                atcatcGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAgg                                                                >  7:35161/1‑139 (MQ=255)
                                                                                                atcatcGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAgg                                                                <  8:35161/139‑1 (MQ=255)
                                                                                                  catcGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCctg                                                     <  5:234387/148‑1 (MQ=255)
                                                                                                      gTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGt                                                                                              <  8:94157/103‑1 (MQ=255)
                                                                                                      gTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGt                                                                                              >  7:94157/1‑103 (MQ=255)
                                                                                                           ccc‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGTTGGACATCGTCAAGTACAGCCAGGACGATGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTcc                                           >  6:14510/1‑149 (MQ=255)
                                                                                                           ccc‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTcc                                           >  5:89559/1‑149 (MQ=255)
                                                                                                           ccc‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTcc                                           >  8:231591/1‑149 (MQ=255)
                                                                                                           ccc‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTcc                                           <  7:128325/149‑1 (MQ=255)
                                                                                                            cc‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCt                                          <  1:138514/149‑1 (MQ=255)
                                                                                                                       gTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATc                                >  6:47841/1‑149 (MQ=255)
                                                                                                                           cATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGCCa                            <  4:368510/149‑1 (MQ=255)
                                                                                                                                tgctgACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGCCAAGGCa                       >  1:231749/1‑149 (MQ=255)
                                                                                                                                  ctgACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCt                                          >  8:99987/1‑128 (MQ=255)
                                                                                                                                    gACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGcc                             <  2:262370/139‑1 (MQ=255)
                                                                                                                                    gACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGcc                             >  1:262370/1‑139 (MQ=255)
                                                                                                                                    gACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGCCAAGGCATTCa                   >  7:179384/1‑149 (MQ=255)
                                                                                                                                       cATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACgaag                                                                               <  4:134236/86‑1 (MQ=255)
                                                                                                                                       cATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACgaag                                                                               >  3:134236/1‑86 (MQ=255)
                                                                                                                                          ggttgCCTTCGGCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGCCAAGGCAt                      >  4:160055/5‑140 (MQ=255)
                                                                                                                                          ggttgCCTTCGGCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGCCAAGGCAt                      <  3:160055/136‑1 (MQ=255)
                                                                                                                                           gttgCCTTCGGCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGCCAAGGCATTCATGACCg             >  6:299939/4‑148 (MQ=255)
                                                                                                                                            aGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGCCAAGGCATTCATGACCGaa           <  7:231591/149‑1 (MQ=255)
                                                                                                                                                 ttCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGCCAAGGCATTCATGACCGAAGTc        <  4:366363/147‑1 (MQ=255)
                                                                                                                                                 ttCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGCCAAGGCATTCATGACCGAAGTc        >  3:366363/1‑147 (MQ=255)
                                                                                                                                                  tCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGCCAAGGCATTCATGACCGAAGTCGGt     >  7:93756/1‑149 (MQ=255)
                                                                                                                                                   cGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGCCAAGGCATTCATGACCGAAGTCGGt     <  5:47841/148‑1 (MQ=255)
                                                                                                                                                     ccGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGCCAAGGCATTCATGACCGAAGTCGGTTTc  <  4:60486/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAGGTGGCGTTCCAGGGTGGCCTGAAGTACGCCCGTGAGCGCCTGCAGATGCGTTCCCTGACCGGCCCGAAAGCACCGGACAAGGCCGCTGACCCGATCATCGTCCACCC‑GGACGTGCGTCGCATGCTGCTGACCATGAAGGCCTTCGCCGAAGGCAACCGCGCGATGGTTTACTTCACCGCCAAGCAGGTGGACATCGTCAAGTACAGCCAGGACGAAGAAGAGCGCAAGAAGGCTGACGCCCTGCTGGCGTTCCTGACCCCGATCGCCAAGGCATTCATGACCGAAGTCGGTTTC  >  NC_002947/446236‑446532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑