| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,295,528 | A→T | 6.7% | intergenic (+59/+67) | PP_4668 → / ← PP_4669 | LysR family transcriptional regulator/OmpA family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,295,528 | 0 | A | T | 6.7% | 182.3 / 2.8 | 60 | intergenic (+59/+67) | PP_4668/PP_4669 | LysR family transcriptional regulator/OmpA family protein |
| Reads supporting (aligned to +/- strand): ref base A (27/29); new base T (2/2); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
CTGTGGGATTACATCCGCGGGGTGACCGAGGCGAATGCGCCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCGGCGCTGGTGCGGTTGTCCGCCACCGGCTGG > NC_002947/5295383‑5295673 | ctgTGGGATTACATCCGCGGGGTGACCGAGGCGAATGCGCCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGc < 5:76619/149‑1 (MQ=255)ctgTGGGATTACATCCGCGGGGTGACCGAGGCGAATGCGCCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCGGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGc > 8:104539/1‑149 (MQ=255) gggATTACATCCGCGGGGTGACCGAGGCGAATGCGCCGTTGTTCATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGt < 2:316970/149‑1 (MQ=255) tACATCCGCGGGGTGACCGAGGCGAATGCGCCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCa < 6:338242/148‑1 (MQ=255) aTCCGCGGGGTGACCGAGGCGAATGCGCCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGaca > 4:212954/1‑149 (MQ=255) gggTGACCGAGGCGAATGCGCCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGtt < 8:1997/149‑1 (MQ=255) gTGACCGAGGCGAATGCGCCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGc < 5:390409/149‑1 (MQ=255) gTGACCGAGGCGAATGCGCCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGc > 7:339021/1‑149 (MQ=255) tGACCGAGGCGAATGCGCCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCa > 8:81978/1‑135 (MQ=255) tGACCGAGGCGAATGCGCCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCa < 7:81978/135‑1 (MQ=255) ccGAGGCGAATGCGCCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCcgc > 6:169415/1‑149 (MQ=255) gAGGCGAATGCGCCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCgcgc < 1:337279/149‑1 (MQ=255) gcCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTg > 7:324106/1‑116 (MQ=255) gcCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTg < 8:324106/116‑1 (MQ=255) gcCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGc > 7:357413/1‑149 (MQ=255) ttgttgATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTAtg > 3:164214/1‑149 (MQ=255) gATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGa > 5:44742/1‑147 (MQ=255) gATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGa > 5:162467/1‑147 (MQ=255) gCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTAt > 2:267349/1‑137 (MQ=255) gCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGccc > 7:218645/1‑147 (MQ=255) gCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGccc > 7:163277/1‑147 (MQ=255) gCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCCAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTAt < 1:267349/137‑1 (MQ=255) aaCCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAgcg < 3:212954/149‑1 (MQ=255) cccGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAgcggc < 6:162467/149‑1 (MQ=255) ccGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGCAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAgcggcg > 7:348518/1‑149 (MQ=255) ccGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAgcggcg < 4:355344/149‑1 (MQ=255) ccGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAgcggc > 4:145900/1‑148 (MQ=255) cgcgAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGcga < 8:357413/149‑1 (MQ=255) agaTGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTAtgt < 4:298159/128‑1 (MQ=255) agaTGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTAtgt > 3:298159/1‑128 (MQ=255) agaTGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGAt < 8:218645/149‑1 (MQ=255) gcgcTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAg > 1:37950/1‑148 (MQ=255) cccAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGc > 3:177531/1‑148 (MQ=255) ccAGGACTGATGGGCCGGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTa < 7:104539/149‑1 (MQ=255) aTGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGAt < 4:319629/147‑1 (MQ=255) aTGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGAt < 6:44742/147‑1 (MQ=255) tGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGa < 3:78762/149‑1 (MQ=255) ccTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACAcc < 3:145900/149‑1 (MQ=255) cATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACgg > 5:115203/1‑149 (MQ=255) aTTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGt > 8:158251/1‑149 (MQ=255) ccGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTtct < 6:323450/148‑1 (MQ=255) aGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGt < 2:37950/149‑1 (MQ=255) ccccccTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTAc < 8:163277/149‑1 (MQ=255) cccccTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACg > 1:194106/1‑149 (MQ=255) ccccTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGc > 8:17821/1‑149 (MQ=255) tGTAGACGCGGTCTGGCCCGCGAACACCGGCGATGCCGGTGCAAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGGGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCgg < 8:339021/149‑1 (MQ=255) gTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCg > 3:39034/1‑87 (MQ=255) gTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCg < 4:39034/87‑1 (MQ=255) tAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCGgcg > 7:102011/1‑149 (MQ=255) gCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCGGCGCTGGTGCgg < 6:115203/149‑1 (MQ=255) cccGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCGGCGCTGGTGCGGTTGTc < 7:158251/149‑1 (MQ=255) tctggcacCGGCTTCGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCGGCGCTGGTGCGGTTGTCCGc > 3:127411/6‑149 (MQ=255) tggcacCGGCTTCGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCGGCGCTGGTGCgg < 8:59002/136‑1 (MQ=255) tggcacCGGCTTCGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCGGCGCTGGGGCgg > 7:59002/4‑139 (MQ=255) aaCACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCGGCGCTGGTGCGGTTGTCCGCCAc < 5:169415/149‑1 (MQ=255) gcacCGGCTTCGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCGGCGCTGGTGCGGTTGTCCGCCAc < 4:127411/147‑1 (MQ=255) acCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCGGCGCTGGTGCGGTTGTCCGCCACCgg > 3:215990/1‑149 (MQ=255) cGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCg > 6:147349/1‑121 (MQ=255) cGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCg < 5:147349/121‑1 (MQ=255) gCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCGGCGCTGGTGCGGTTGTCCGCCACCggctgg > 6:63642/1‑149 (MQ=255) | CTGTGGGATTACATCCGCGGGGTGACCGAGGCGAATGCGCCGTTGTTGATGGGGCAAACCCGCGAGATGCGCTTTGCCCAGGACTGATGGGCCTGCGCATTTCCGAGCCCCCCTGTAGAAGCGGGCTTGCCCGCGAACACCGGCGAAGCCGGTGCCAGACACCGCGTTGCCCGCGCCTACAGGGGGCTATGTGACAGCCCGAGCGGCGACGATCAGTAGGACGCTACCACGATGGACACCCGACGGTTCTCGGTACGCCCGGCGCTGGTGCGGTTGTCCGCCACCGGCTGG > NC_002947/5295383‑5295673 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |