Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,324,514 C→T 6.2% A383V (GCC→GTC)  PP_4684 → kinase‑like domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,324,5140CT 6.2% 193.1 / 2.3 64A383V (GCC→GTC) PP_4684kinase‑like domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (27/33);  new base T (2/2);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GTCGCGCCTGCTGGCCATCACCCATGGCGTTTCGGCGGTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCGGTGGTGCTGGACGCCACCTACCTCAAGCGT  >  NC_002947/5324368‑5324644
                                                                                                                                                  |                                                                                                                                  
gTCGCGCCTGCTGGCCATCACCCATGGCGTTTCGGCGGTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCg                                                                                                                                  <  2:142588/149‑1 (MQ=255)
    cgcCTGCTGGCCATCACCCATGGCGTTTCGGCGGTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGc                                                                                                                                >  2:14401/1‑147 (MQ=255)
       ctgctgGCCATCACCCATGGCGTTTCGGCGGTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCt                                                                                                                           <  1:14401/149‑1 (MQ=255)
          ctgGCCATCACCCATGGCGTTTCGGCGGTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGa                                                                                                                         <  1:167535/148‑1 (MQ=255)
               cATCACCCATGGCGTTTCGGCGGTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCg                                                                                                                   <  7:49812/149‑1 (MQ=255)
                aTCACCCATGGCGTTTCGGCGGTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGGCGTCGGCCTGcggttgtt                                                                                                                     >  8:229655/1‑139 (MQ=255)
                aTCACCCATGGCGTTTCGGCGGTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGGCGTCGGCCTGcggttgtt                                                                                                                     <  7:229655/146‑8 (MQ=255)
                    cccATGGCGTTTCGGCGGTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCata                                                                                                              <  2:136306/149‑1 (MQ=255)
                     ccATGGCGTTTCGGCGGTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCatat                                                                                                             <  3:69895/149‑1 (MQ=255)
                     ccATGGCGTTTCGGCGGTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCata                                                                                                              >  5:261595/1‑148 (MQ=255)
                         ggCGTTTCGGCGGTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACg                                                                                                          <  6:261595/148‑1 (MQ=255)
                               tCGGCGGTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAgg                                                                                                    <  5:201336/148‑1 (MQ=255)
                                  gcggTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGc                                                                                                <  3:378/149‑1 (MQ=255)
                                    ggTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCca                                                                                              <  5:4336/149‑1 (MQ=255)
                                           aaaaGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCata                                                                                                              >  1:367575/1‑126 (MQ=255)
                                           aaaaGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCata                                                                                                              <  2:367575/126‑1 (MQ=255)
                                            aaaGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCcagcgcagc                                                                                       >  4:312936/1‑148 (MQ=255)
                                              aGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGAc                                                                                    <  2:134609/149‑1 (MQ=255)
                                               gTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGAcc                                                                                   >  1:124768/1‑149 (MQ=255)
                                                tCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGAcc                                                                                   >  2:245680/1‑148 (MQ=255)
                                                     tGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTg                                                                                                                          >  1:165698/1‑104 (MQ=255)
                                                     tGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTg                                                                                                                          <  2:165698/104‑1 (MQ=255)
                                                       gCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAgc                                                                            <  3:312936/148‑1 (MQ=255)
                                                             cgcCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGc                                                                      <  4:181206/148‑1 (MQ=255)
                                                              gcCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCAc                                                                    >  5:332761/1‑149 (MQ=255)
                                                              gcCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCAc                                                                    >  1:20265/1‑149 (MQ=255)
                                                                     cGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTag                                                             >  6:169708/1‑149 (MQ=255)
                                                                      gAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTag                                                             >  4:299712/1‑148 (MQ=255)
                                                                        ggCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGACCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTagcag                                                          >  4:341989/1‑149 (MQ=255)
                                                                         gCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACTTATCAGCGCCTGCACGAGGTagcagc                                                         <  1:204558/149‑1 (MQ=255)
                                                                            cTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCa                                                                                                                              <  2:12302/77‑1 (MQ=255)
                                                                            cTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCa                                                                                                                              >  1:12302/1‑77 (MQ=255)
                                                                               ggTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTag                                                             >  3:212790/1‑139 (MQ=255)
                                                                               ggTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTag                                                             <  4:212790/139‑1 (MQ=255)
                                                                                gTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAgcgc                                                                          <  2:200141/125‑1 (MQ=255)
                                                                                gTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAgcgc                                                                          >  1:200141/1‑125 (MQ=255)
                                                                                gTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGtg                                                  <  1:245680/149‑1 (MQ=255)
                                                                                     gTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTgcg                                             >  2:202681/1‑149 (MQ=255)
                                                                                          cgtgcgtTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCg                                        >  4:325260/1‑149 (MQ=255)
                                                                                                  cGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCg                                >  4:191893/1‑149 (MQ=255)
                                                                                                   ggACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCg                                                                                                                   >  1:354199/1‑65 (MQ=255)
                                                                                                   ggACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCg                                                                                                                   <  2:354199/65‑1 (MQ=255)
                                                                                                   ggACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCgg                               >  5:188951/1‑149 (MQ=255)
                                                                                                    gACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTTCGCGCCGGCTTCCCggt                              <  4:45721/149‑1 (MQ=255)
                                                                                                     aCGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCggtg                             <  6:332761/149‑1 (MQ=255)
                                                                                                     aCGGAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCTGGACGCCAGCGCAGCTACCTATCAGCGCCTGCACGAGGTagc                                                            <  6:170081/118‑1 (MQ=255)
                                                                                                      cGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGcc                                         <  5:278610/136‑1 (MQ=255)
                                                                                                      cGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGcc                                         >  6:278610/1‑136 (MQ=255)
                                                                                                       gTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCggtggt                           >  4:163025/1‑149 (MQ=255)
                                                                                                         agagCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCgg                               <  2:199555/143‑1 (MQ=255)
                                                                                                         agagCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCgg                               >  1:199555/1‑143 (MQ=255)
                                                                                                                 aGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTcc                                  >  8:209451/1‑132 (MQ=255)
                                                                                                                 aGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTcc                                  <  7:209451/132‑1 (MQ=255)
                                                                                                                    gcCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCGGTGGTGCTGGACGCCacc              >  2:354584/1‑149 (MQ=255)
                                                                                                                     ccTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCGGTGGTGCTGGACGCCacct             <  2:82577/149‑1 (MQ=255)
                                                                                                                     ccTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCGGTGGTGCTGGACGCCacc              >  1:82577/1‑148 (MQ=255)
                                                                                                                         ttCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCGGTGGTGCTGGACGCCacctac           <  2:20265/147‑1 (MQ=255)
                                                                                                                          tCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCGGTGGTGCTGGACGCCacctacct         <  3:163025/148‑1 (MQ=255)
                                                                                                                            ggCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCGGTGGTGCTGGACGCCACCTACCTCaa      <  3:325260/149‑1 (MQ=255)
                                                                                                                               gAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCGgtggtg                          <  6:29724/126‑1 (MQ=255)
                                                                                                                               gAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCGgtggtg                          >  5:29724/1‑126 (MQ=255)
                                                                                                                                aacaacCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCGGTGGTGCTGGACGCCACCTACCTCAAGCGt  <  5:169708/149‑1 (MQ=255)
                                                                                                                                       gctggCCGGCGTCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCGGTGGTGCTGGACGCCACCTACCTCaa      >  7:273767/4‑138 (MQ=255)
                                                                                                                                       gctggCCGGCGTCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCGGTGGTGCTGGACGCCACCTACCTCaa      <  8:273767/135‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                  
GTCGCGCCTGCTGGCCATCACCCATGGCGTTTCGGCGGTGGGCAAAAGTCACGTGGCCATGCGCCTGGTCGAGGCCCTGGGTGCAGTGCGCGTGCGTTCGGACGTAGAGCGTAAGCGCCTGTTCGGCGAACAACCGCAGGCCGACGCCGGCCAGCTGAGTACCGGCATATACGACCAGGACGCCAGCGCAGCGACCTATCAGCGCCTGCACGAGGTAGCAGCGACCGTGCTGCGCGCCGGCTTCCCGGTGGTGCTGGACGCCACCTACCTCAAGCGT  >  NC_002947/5324368‑5324644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑