Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,364,420	C→G	7.3%	G72R (GGT→CGT)	folP ←	dihydropteroate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,364,420	0	C	G	7.3%	196.4 / 2.6	64	G72R (GGT→CGT)	folP	dihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base C (32/27);  new base G (2/3);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGG  >  NC_002947/5364273‑5364568                                                                                                                                                    |                                                                                                                                                      tCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCAccc                                                                                                                                                      <  8:42603/149‑1 (MQ=255)     cccGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCAAGACTGCACGCGCACCCGGgc                                                                                                                                                  >  1:352555/1‑149 (MQ=255)         gCCCCCAGGCGCTCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCTAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGt                                                                                                                                              <  2:305307/149‑1 (MQ=255)         gCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGt                                                                                                                                              <  4:304314/149‑1 (MQ=255)         gCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGt                                                                                                                                              <  5:64017/149‑1 (MQ=255)         gCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGt                                                                                                                                              <  3:170565/149‑1 (MQ=255)         gCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCAAGACTGCACGCGCACCCGGGCGCGt                                                                                                                                              <  2:352555/149‑1 (MQ=255)           ccccAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTgg                                                                                                                                            >  8:59919/1‑149 (MQ=255)           ccccAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTgg                                                                                                                                            <  4:36303/149‑1 (MQ=255)             ccAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCCCGGGTGCGCGTgcag                                                                                                                                          <  2:308764/149‑4 (MQ=255)             ccAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGAc                                                                                                                                          >  5:16856/1‑149 (MQ=255)                    cgcgGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCAcc                                                                                                                                                       <  8:356851/129‑1 (MQ=255)                    cgcgGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCAcc                                                                                                                                                       >  7:356851/1‑129 (MQ=255)                     gcgGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCAcc                                                                                                                                 <  4:366466/149‑1 (MQ=255)                     gcgGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCAcc                                                                                                                                 >  3:346173/1‑149 (MQ=255)                      cgGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCAcc                                                                                                                                 >  6:95558/1‑148 (MQ=255)                               gCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATc                                                                                                                       <  5:95558/149‑1 (MQ=255)                                cATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCa                                                                                                                      <  5:344548/149‑1 (MQ=255)                                  tGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAAc                                                                                                                    <  3:64902/149‑1 (MQ=255)                                        cagGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTgcg                                                                                                              >  1:282619/1‑149 (MQ=255)                                         agGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTgcgc                                                                                                             >  8:210293/1‑149 (MQ=255)                                               ggAGGTATGGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCcggcgg                                                                                                       >  3:330163/1‑149 (MQ=255)                                                        gACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATc                                                                                               <  7:210293/148‑1 (MQ=255)                                                         aCCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGc                                                                                             >  1:326048/1‑149 (MQ=255)                                                            gAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTc                                                                                          <  6:16856/149‑1 (MQ=255)                                                                  aCCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGt                                                                                     <  4:330163/148‑1 (MQ=255)                                                                  aCCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTg                                                                                    >  3:87068/1‑149 (MQ=255)                                                                    cACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGgc                                                                                  >  8:94618/1‑149 (MQ=255)                                                                        tCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAg                                                                              >  4:238692/1‑149 (MQ=255)                                                                          gAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTg                                                                                    <  4:361521/141‑1 (MQ=255)                                                                          gAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTg                                                                                    >  3:361521/1‑141 (MQ=255)                                                                           aGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGc                                                                           <  7:94618/149‑1 (MQ=255)                                                                            ggCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGcc                                                                          <  2:231611/149‑1 (MQ=255)                                                                                   gTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTcgc                                                                   >  1:104874/1‑149 (MQ=255)                                                                                    ttGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCAACGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTcgcg                                                                  >  1:83132/1‑149 (MQ=255)                                                                                        tGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGCCTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                                                              >  7:154029/1‑149 (MQ=255)                                                                                        tGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                                                              >  1:101563/1‑149 (MQ=255)                                                                                             tCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGaa                                                         >  6:109930/1‑149 (MQ=255)                                                                                               gACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCCCCGCGATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGCAGc                                                       >  3:354453/1‑149 (MQ=255)                                                                                               gACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGaa                                                         <  2:83132/147‑1 (MQ=255)                                                                                                  cATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGAc                                                    >  7:32417/1‑149 (MQ=255)                                                                                                        cgcCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCAt                                              >  6:314787/1‑149 (MQ=255)                                                                                                         gcCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATc                                             >  2:229290/1‑149 (MQ=255)                                                                                                          ccACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCg                                            <  1:229290/149‑1 (MQ=255)                                                                                                            acacGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCg                                            >  6:377120/1‑147 (MQ=255)                                                                                                                gCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAg                                      <  4:346173/149‑1 (MQ=255)                                                                                                                gCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAg                                      >  5:93369/1‑149 (MQ=255)                                                                                                                   gtgcagtctcGGTGACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGaa                                   >  7:192410/8‑149 (MQ=255)                                                                                                                    cAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACTGCCATCGGAGAAGGaa                                   <  7:59919/148‑1 (MQ=255)                                                                                                                      gCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATc                                 >  3:45002/1‑148 (MQ=255)                                                                                                                      gCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATc                                 <  4:45002/148‑1 (MQ=255)                                                                                                                       ctcctcGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCgg                               <  1:38394/149‑1 (MQ=255)                                                                                                                        tcctcGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCggg                              >  7:96171/1‑149 (MQ=255)                                                                                                                          ctcGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCggggg                            >  6:182303/1‑149 (MQ=255)                                                                                                                            cGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAAt                                  >  8:256186/1‑141 (MQ=255)                                                                                                                            cGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAAt                                  <  7:256186/141‑1 (MQ=255)                                                                                                                             ggTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGt                           <  3:238692/147‑1 (MQ=255)                                                                                                                             ggTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGt                           <  5:109930/147‑1 (MQ=255)                                                                                                                                 aCCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACAATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGACGCGACCGCCCTCGCAGAAGGAATCGGGGGTGatat                      >  1:248544/1‑149 (MQ=255)                                                                                                                                   cGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTga                   <  5:314787/149‑1 (MQ=255)                                                                                                                                       agtccACGCGCCCGGGTGCGCGTGCAGTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAGCCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTgag                  >  7:242350/5‑146 (MQ=255)                                                                                                                                       agtccACGCGCCCGGGTGCGCGTGCAGTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTgag                  <  8:242350/142‑1 (MQ=255)                                                                                                                                        gtccACGCGCCCGGGTGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCggggg                            <  6:84070/132‑1 (MQ=255)                                                                                                                                        gtccACGCGCCCGGGTGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCggggg                            >  5:84070/4‑135 (MQ=255)                                                                                                                                             cgcgCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCAt         <  3:24036/149‑1 (MQ=255)                                                                                                                                                  aCCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACAt    >  7:6688/1‑149 (MQ=255)                                                                                                                                                  aCCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACAt    >  4:67783/1‑149 (MQ=255)                                                                                                                                                   cccGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATg   <  4:87068/149‑1 (MQ=255)                                                                                                                                                    ccGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATgg  >  2:108362/1‑149 (MQ=255)                                                                                                                                                    ccGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATgg  <  6:93369/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      TCAGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTC‑GCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGG  >  NC_002947/5364273‑5364568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑