Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,463,400	T→A	7.4%	intergenic (‑51/+48)	PP_4799 ← / ← lipA	muramoyltetrapeptide carboxypeptidase/lipoyl synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,463,400	0	T	A	7.4%	285.1 / 9.7	95	intergenic (‑51/+48)	PP_4799/lipA	muramoyltetrapeptide carboxypeptidase/lipoyl synthase
Reads supporting (aligned to +/- strand):  ref base T (44/44);  new base A (3/4);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

TCAAGCCGCGCCGCGCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTT  >  NC_002947/5463252‑5463548                                                                                                                                                      |                                                                                                                                                       tCAAGCCGCGCCGCGCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCt                                                                                                                                                        <  2:98588/149‑1 (MQ=255)     gccgcgccgcGCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTggg                                                                                                                                                    <  7:59495/149‑1 (MQ=255)      ccgcgccgcgCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGgc                                                                                                                                                   >  1:167262/1‑149 (MQ=255)         cgccgcgccCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGgcgcg                                                                                                                                                <  8:190250/149‑1 (MQ=255)           ccgcgccCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCa                                                                                                                                              >  2:160865/1‑149 (MQ=255)            cgcgccCGCCGGGGCGACAATGGCAAATCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGgc                                                                                                                                                   <  4:68738/143‑1 (MQ=255)            cgcgccCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCGCGCCCAAGGgcggcat                                                                                                                                              >  6:254041/1‑145 (MQ=255)            cgcgccCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGgc                                                                                                                                                   >  3:68738/1‑143 (MQ=255)             gcgccCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATc                                                                                                                                            <  1:160865/149‑1 (MQ=255)              cgcccgccGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCg                                                                                                                                           <  2:154397/149‑1 (MQ=255)              cgcccgccGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCg                                                                                                                                           >  2:136110/1‑149 (MQ=255)              cgcccgccGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCg                                                                                                                                           <  8:306448/149‑1 (MQ=255)                cccgccGGGGCGACAATGGCAAACGATGCGTTGGCCGTCAGCGCGGCTGGCAGTGTGGGTTGGAAGGTTTTGGCGCTGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGGCTATGCGCGCCCAAGGGCG‑GCATCGGc                                                                                                                                         <  8:62914/149‑1 (MQ=255)                cccgccGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCGCGCCCAAGGGCG‑GCATCGGc                                                                                                                                         <  5:254041/149‑1 (MQ=255)                   gccgGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCGCGCCCAAGGGCG‑GCATCGGc                                                                                                                                         <  4:127534/146‑1 (MQ=255)                   gccgGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCGCGCCCAAGGGCG‑GCATCGGc                                                                                                                                         >  3:127534/1‑146 (MQ=255)                      ggggCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCtgt                                                                                                                                   <  3:144785/149‑1 (MQ=255)                       gggCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCtgtg                                                                                                                                  <  4:318709/149‑1 (MQ=255)                        ggCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCGCGCCCAAGGGcg‑gcatcggcatttttgt                                                                                                                                 <  4:59602/149‑7 (MQ=38)                           gACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTc                                                                                                                              <  6:172596/149‑1 (MQ=255)                                tGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGctc                                                                                                                         >  7:359939/1‑149 (MQ=255)                                  gCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCAt                                                                                                                                             <  3:257365/127‑1 (MQ=255)                                  gCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCAt                                                                                                                                             >  4:257365/1‑127 (MQ=255)                                    aaaaCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTa                                                                                                                       >  5:114108/1‑147 (MQ=255)                                    aaaaCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTa                                                                                                                       <  6:114108/147‑1 (MQ=255)                                     aaaCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGc                                                                                                                    >  8:179802/1‑149 (MQ=255)                                     aaaCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGc                                                                                                                    <  5:58681/149‑1 (MQ=255)                                     aaaCATGCGCTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGc                                                                                                                    <  5:53036/149‑1 (MQ=255)                                         aTGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTaga                                                                                                                <  2:167262/149‑1 (MQ=255)                                         aTGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTaga                                                                                                                <  6:143238/149‑1 (MQ=255)                                           gCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTagag                                                                                                               >  1:277954/1‑148 (MQ=255)                                            cGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCa                                                                                                             <  3:80258/149‑1 (MQ=255)                                             gTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCAt                                                                                                            <  6:222262/149‑1 (MQ=255)                                                  ccGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTgg                                                                                                                                                     >  2:26007/1‑103 (MQ=255)                                                   cGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCa                                                                                                     <  5:29498/149‑1 (MQ=255)                                                     gCATCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGAGCAGTACATCGCGGCTCATTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCaga                                                                                                   <  8:206801/149‑1 (MQ=255)                                                      cAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGc                                                                                                                                         >  7:135756/1‑111 (MQ=255)                                                      cAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGc                                                                                                                                         <  8:135756/111‑1 (MQ=255)                                                      cAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCagag                                                                                                  <  7:92953/149‑1 (MQ=255)                                                          gcgGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCtt                                                                                               >  5:109577/1‑148 (MQ=255)                                                                 gCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCtgt                                                                                                                                   >  5:364775/1‑106 (MQ=255)                                                                 gCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCtgt                                                                                                                                   <  6:364775/106‑1 (MQ=255)                                                                 gCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTg                                                                                        <  2:283211/148‑1 (MQ=255)                                                                   aGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCAGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCt                                                                                     >  5:23565/1‑149 (MQ=255)                                                                       tgggtTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGt                                                                                 >  3:264222/1‑149 (MQ=255)                                                                         ggtTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTgg                                                                               <  6:109577/149‑1 (MQ=255)                                                                           ttGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGc                                                                             >  1:314576/1‑149 (MQ=255)                                                                           ttGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGc                                                                             >  3:373585/1‑149 (MQ=255)                                                                              gAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCctg                                                                          >  8:185230/1‑149 (MQ=255)                                                                              gAAGGTATTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCctg                                                                          >  4:82699/1‑149 (MQ=255)                                                                               aaGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCctgc                                                                         <  7:185230/149‑1 (MQ=255)                                                                                 ggTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCAGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGCCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCctgctg                                                                       >  3:190162/1‑149 (MQ=255)                                                                                 gcttttGGCGCAGTCCATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCctgctg                                                                       <  8:335066/147‑1 (MQ=255)                                                                                  gTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTgg                                                                      >  2:4541/1‑149 (MQ=255)                                                                                          gcAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTg                                                              >  8:98253/1‑149 (MQ=255)                                                                                           cAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAg                                                                                                                      >  6:382210/1‑93 (MQ=255)                                                                                           cAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAg                                                                                                                      <  5:382210/93‑1 (MQ=255)                                                                                            aGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGt                                                            <  1:136110/149‑1 (MQ=255)                                                                                            aGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGt                                                            <  4:264222/149‑1 (MQ=255)                                                                                                 aTCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCg                                                                >  2:355351/1‑140 (MQ=255)                                                                                                 aTCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCg                                                                <  1:355351/140‑1 (MQ=255)                                                                                                 aTCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTa                                                           <  4:43369/145‑1 (MQ=255)                                                                                                 aTCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTa                                                           >  3:43369/1‑145 (MQ=255)                                                                                                 aTCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaa                                                       <  8:359939/149‑1 (MQ=255)                                                                                                 aTCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaa                                                       >  6:30447/1‑149 (MQ=255)                                                                                                   cgcgGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaag                                                      >  6:277157/1‑148 (MQ=255)                                                                                                   cgcgGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATAGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaag                                                      >  5:162273/1‑148 (MQ=255)                                                                                                    gcgGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAAAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaagaa                                                    >  6:217960/1‑149 (MQ=255)                                                                                                      ggCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACg                                                  <  3:82699/149‑1 (MQ=255)                                                                                                         tCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTg                                                              >  8:41715/1‑134 (MQ=255)                                                                                                         tCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTg                                                              <  7:41715/134‑1 (MQ=255)                                                                                                         tCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCAc                                               >  7:35834/1‑149 (MQ=255)                                                                                                           cTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCAc                                               <  5:30447/147‑1 (MQ=255)                                                                                                               cAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTGGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCg‑g                                        >  3:15602/1‑149 (MQ=255)                                                                                                                aGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCg‑gg                                       <  4:253101/149‑1 (MQ=255)                                                                                                                    cATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAAAGCACCAGCG‑GGc                                      >  3:111977/1‑146 (MQ=255)                                                                                                                            acaaacaaAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACg                           >  2:305969/1‑149 (MQ=255)                                                                                                                              aaacaaaAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGtt                         <  7:290588/149‑1 (MQ=255)                                                                                                                              aaacaaaAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGtt                         >  2:40237/1‑149 (MQ=255)                                                                                                                               aacaaaAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTc                        >  3:260188/1‑149 (MQ=255)                                                                                                                                acaaaAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCt                       >  4:99391/1‑149 (MQ=255)                                                                                                                                acaaaAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCt                       >  8:80677/1‑149 (MQ=255)                                                                                                                                 caaaAATGCCGATGC‑CGCCGTTGGGCGGGCATGGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCtt                      <  2:277954/149‑1 (MQ=255)                                                                                                                                    aaaTGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTg                     >  1:303358/1‑148 (MQ=255)                                                                                                                                     aaTGCGGATGC‑CGCCCTTGGGCGCGCATCGTCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCATAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAg                  <  6:23565/149‑1 (MQ=255)                                                                                                                                       tgccgatgc‑cgCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGcc                >  5:349870/1‑149 (MQ=255)                                                                                                                                        gccgatgc‑cgCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGcc                >  8:314125/1‑148 (MQ=255)                                                                                                                                           gatgc‑cgCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATc            <  5:277157/149‑1 (MQ=255)                                                                                                                                             tgcgcgccCAAGGGCG‑GCATCGGCATCAGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCtt          >  3:212674/5‑149 (MQ=255)                                                                                                                                               c‑cgccCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGAGGCCAGAAGCAACGTTCTTGAAGCCCATCtt          <  1:40237/148‑1 (MQ=255)                                                                                                                                               c‑cgccCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCTTGt        <  7:80677/149‑1 (MQ=255)                                                                                                                                                 cgccCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCTTGt        >  6:185651/1‑148 (MQ=255)                                                                                                                                                  gccCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTaa      >  5:323897/1‑149 (MQ=255)                                                                                                                                                    ccTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAAcc    <  5:185651/149‑1 (MQ=255)                                                                                                                                                     cTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCg                                                                <  3:353422/89‑1 (MQ=255)                                                                                                                                                     cTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCg                                                                >  4:353422/1‑89 (MQ=255)                                                                                                                                                      ttGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCtt  >  5:41563/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                       TCAAGCCGCGCCGCGCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTT  >  NC_002947/5463252‑5463548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑