Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,463,401 T→A 7.4% intergenic (‑52/+47) PP_4799 ← / ← lipA muramoyltetrapeptide carboxypeptidase/lipoyl synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,463,4010TA 7.4% 279.9 / 9.8 95intergenic (‑52/+47)PP_4799/lipAmuramoyltetrapeptide carboxypeptidase/lipoyl synthase
Reads supporting (aligned to +/- strand):  ref base T (44/44);  new base A (3/4);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.95e-01

GCCGCGCCGCGCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTT  >  NC_002947/5463256‑5463548
                                                                                                                                                  |                                                                                                                                                     
gccgcgccgcGCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTggg                                                                                                                                                    <  7:59495/149‑1 (MQ=255)
 ccgcgccgcgCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGgc                                                                                                                                                   >  1:167262/1‑149 (MQ=255)
    cgccgcgccCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGgcgcg                                                                                                                                                <  8:190250/149‑1 (MQ=255)
      ccgcgccCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCa                                                                                                                                              >  2:160865/1‑149 (MQ=255)
       cgcgccCGCCGGGGCGACAATGGCAAATCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGgc                                                                                                                                                   <  4:68738/143‑1 (MQ=255)
       cgcgccCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCGCGCCCAAGGgcggcat                                                                                                                                              >  6:254041/1‑145 (MQ=255)
       cgcgccCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGgc                                                                                                                                                   >  3:68738/1‑143 (MQ=255)
        gcgccCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATc                                                                                                                                            <  1:160865/149‑1 (MQ=255)
         cgcccgccGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCg                                                                                                                                           <  8:306448/149‑1 (MQ=255)
         cgcccgccGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCg                                                                                                                                           >  2:136110/1‑149 (MQ=255)
         cgcccgccGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCg                                                                                                                                           <  2:154397/149‑1 (MQ=255)
           cccgccGGGGCGACAATGGCAAACGATGCGTTGGCCGTCAGCGCGGCTGGCAGTGTGGGTTGGAAGGTTTTGGCGCTGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGGCTATGCGCGCCCAAGGGCG‑GCATCGGc                                                                                                                                         <  8:62914/149‑1 (MQ=255)
           cccgccGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCGCGCCCAAGGGCG‑GCATCGGc                                                                                                                                         <  5:254041/149‑1 (MQ=255)
              gccgGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCGCGCCCAAGGGCG‑GCATCGGc                                                                                                                                         >  3:127534/1‑146 (MQ=255)
              gccgGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCGCGCCCAAGGGCG‑GCATCGGc                                                                                                                                         <  4:127534/146‑1 (MQ=255)
                 ggggCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCtgt                                                                                                                                   <  3:144785/149‑1 (MQ=255)
                  gggCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCtgtg                                                                                                                                  <  4:318709/149‑1 (MQ=255)
                   ggCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCGCGCCCAAGGGcg‑gcatcggcatttttgt                                                                                                                                 <  4:59602/149‑7 (MQ=38)
                      gACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTc                                                                                                                              <  6:172596/149‑1 (MQ=255)
                           tGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGctc                                                                                                                         >  7:359939/1‑149 (MQ=255)
                             gCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCAt                                                                                                                                             >  4:257365/1‑127 (MQ=255)
                             gCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCAt                                                                                                                                             <  3:257365/127‑1 (MQ=255)
                               aaaaCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTa                                                                                                                       <  6:114108/147‑1 (MQ=255)
                               aaaaCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTa                                                                                                                       >  5:114108/1‑147 (MQ=255)
                                aaaCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGc                                                                                                                    <  5:58681/149‑1 (MQ=255)
                                aaaCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGc                                                                                                                    >  8:179802/1‑149 (MQ=255)
                                aaaCATGCGCTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGc                                                                                                                    <  5:53036/149‑1 (MQ=255)
                                    aTGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTaga                                                                                                                <  2:167262/149‑1 (MQ=255)
                                    aTGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTaga                                                                                                                <  6:143238/149‑1 (MQ=255)
                                      gCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTagag                                                                                                               >  1:277954/1‑148 (MQ=255)
                                       cGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCa                                                                                                             <  3:80258/149‑1 (MQ=255)
                                        gTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCAt                                                                                                            <  6:222262/149‑1 (MQ=255)
                                             ccGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTgg                                                                                                                                                     >  2:26007/1‑103 (MQ=255)
                                              cGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCa                                                                                                     <  5:29498/149‑1 (MQ=255)
                                                gCATCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGAGCAGTACATCGCGGCTCATTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCaga                                                                                                   <  8:206801/149‑1 (MQ=255)
                                                 cAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGc                                                                                                                                         <  8:135756/111‑1 (MQ=255)
                                                 cAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGc                                                                                                                                         >  7:135756/1‑111 (MQ=255)
                                                 cAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCagag                                                                                                  <  7:92953/149‑1 (MQ=255)
                                                     gcgGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCtt                                                                                               >  5:109577/1‑148 (MQ=255)
                                                            gCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCtgt                                                                                                                                   >  5:364775/1‑106 (MQ=255)
                                                            gCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCtgt                                                                                                                                   <  6:364775/106‑1 (MQ=255)
                                                            gCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTg                                                                                        <  2:283211/148‑1 (MQ=255)
                                                              aGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCAGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCt                                                                                     >  5:23565/1‑149 (MQ=255)
                                                                  tgggtTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGt                                                                                 >  3:264222/1‑149 (MQ=255)
                                                                    ggtTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTgg                                                                               <  6:109577/149‑1 (MQ=255)
                                                                      ttGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGc                                                                             >  3:373585/1‑149 (MQ=255)
                                                                      ttGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGc                                                                             >  1:314576/1‑149 (MQ=255)
                                                                         gAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCctg                                                                          >  8:185230/1‑149 (MQ=255)
                                                                         gAAGGTATTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCctg                                                                          >  4:82699/1‑149 (MQ=255)
                                                                          aaGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCctgc                                                                         <  7:185230/149‑1 (MQ=255)
                                                                            ggTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCAGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGCCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCctgctg                                                                       >  3:190162/1‑149 (MQ=255)
                                                                            gcttttGGCGCAGTCCATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCctgctg                                                                       <  8:335066/147‑1 (MQ=255)
                                                                             gTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTgg                                                                      >  2:4541/1‑149 (MQ=255)
                                                                                     gcAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTg                                                              >  8:98253/1‑149 (MQ=255)
                                                                                      cAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAg                                                                                                                      >  6:382210/1‑93 (MQ=255)
                                                                                      cAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAg                                                                                                                      <  5:382210/93‑1 (MQ=255)
                                                                                       aGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGt                                                            <  1:136110/149‑1 (MQ=255)
                                                                                       aGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGt                                                            <  4:264222/149‑1 (MQ=255)
                                                                                            aTCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCg                                                                >  2:355351/1‑140 (MQ=255)
                                                                                            aTCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCg                                                                <  1:355351/140‑1 (MQ=255)
                                                                                            aTCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTa                                                           <  4:43369/145‑1 (MQ=255)
                                                                                            aTCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTa                                                           >  3:43369/1‑145 (MQ=255)
                                                                                            aTCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaa                                                       <  8:359939/149‑1 (MQ=255)
                                                                                            aTCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaa                                                       >  6:30447/1‑149 (MQ=255)
                                                                                              cgcgGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaag                                                      >  6:277157/1‑148 (MQ=255)
                                                                                              cgcgGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATAGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaag                                                      >  5:162273/1‑148 (MQ=255)
                                                                                               gcgGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAAAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaagaa                                                    >  6:217960/1‑149 (MQ=255)
                                                                                                 ggCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACg                                                  <  3:82699/149‑1 (MQ=255)
                                                                                                    tCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTg                                                              >  8:41715/1‑134 (MQ=255)
                                                                                                    tCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTg                                                              <  7:41715/134‑1 (MQ=255)
                                                                                                    tCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCAc                                               >  7:35834/1‑149 (MQ=255)
                                                                                                      cTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCAc                                               <  5:30447/147‑1 (MQ=255)
                                                                                                          cAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTGGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCg‑g                                        >  3:15602/1‑149 (MQ=255)
                                                                                                           aGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCg‑gg                                       <  4:253101/149‑1 (MQ=255)
                                                                                                               cATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAAAGCACCAGCG‑GGc                                      >  3:111977/1‑146 (MQ=255)
                                                                                                                       acaaacaaAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACg                           >  2:305969/1‑149 (MQ=255)
                                                                                                                         aaacaaaAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGtt                         <  7:290588/149‑1 (MQ=255)
                                                                                                                         aaacaaaAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGtt                         >  2:40237/1‑149 (MQ=255)
                                                                                                                          aacaaaAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTc                        >  3:260188/1‑149 (MQ=255)
                                                                                                                           acaaaAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCt                       >  4:99391/1‑149 (MQ=255)
                                                                                                                           acaaaAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCt                       >  8:80677/1‑149 (MQ=255)
                                                                                                                            caaaAATGCCGATGC‑CGCCGTTGGGCGGGCATGGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCtt                      <  2:277954/149‑1 (MQ=255)
                                                                                                                               aaaTGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTg                     >  1:303358/1‑148 (MQ=255)
                                                                                                                                aaTGCGGATGC‑CGCCCTTGGGCGCGCATCGTCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCATAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAg                  <  6:23565/149‑1 (MQ=255)
                                                                                                                                  tgccgatgc‑cgCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGcc                >  5:349870/1‑149 (MQ=255)
                                                                                                                                   gccgatgc‑cgCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGcc                >  8:314125/1‑148 (MQ=255)
                                                                                                                                      gatgc‑cgCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATc            <  5:277157/149‑1 (MQ=255)
                                                                                                                                        tgcgcgccCAAGGGCG‑GCATCGGCATCAGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCtt          >  3:212674/5‑149 (MQ=255)
                                                                                                                                          c‑cgccCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGAGGCCAGAAGCAACGTTCTTGAAGCCCATCtt          <  1:40237/148‑1 (MQ=255)
                                                                                                                                          c‑cgccCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCTTGt        <  7:80677/149‑1 (MQ=255)
                                                                                                                                            cgccCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCTTGt        >  6:185651/1‑148 (MQ=255)
                                                                                                                                             gccCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTaa      >  5:323897/1‑149 (MQ=255)
                                                                                                                                               ccTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAAcc    <  5:185651/149‑1 (MQ=255)
                                                                                                                                                cTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCg                                                                <  3:353422/89‑1 (MQ=255)
                                                                                                                                                cTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCg                                                                >  4:353422/1‑89 (MQ=255)
                                                                                                                                                 ttGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCtt  >  5:41563/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCCGCGCCGCGCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATA‑GCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCG‑GGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTT  >  NC_002947/5463256‑5463548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑