Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,469,180 A→T 14.8% L273H (CTC→CAC)  mrdB ← murein transpeptidase factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,469,1800AT14.8% 126.7 / 13.0 54L273H (CTC→CAC) mrdBmurein transpeptidase factor
Reads supporting (aligned to +/- strand):  ref base A (25/21);  new base T (4/4);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

TACAAAAAAAGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCAACGGGTCG  >  NC_002947/5469035‑5469326
                                                                                                                                                  |                                                                                                                                                  
taCAAAAAAAGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCa                                                                                                                                                 >  3:243765/1‑149 (MQ=255)
        aaGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCgatg                                                                                                                                         <  4:243765/149‑1 (MQ=255)
         aGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGTGCTCGGCgagga                                                                                                                                        <  1:268551/149‑1 (MQ=255)
             aTGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGaa                                                                                                                                    <  3:335545/149‑1 (MQ=255)
               ggTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGt                                                                                                                                  >  2:55777/1‑149 (MQ=255)
                 tCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACg                                                                                                                                               <  8:233279/134‑1 (MQ=255)
                 tCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACg                                                                                                                                               >  7:233279/1‑134 (MQ=255)
                 tCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGatgatg                                                                                                                                      >  1:127580/1‑143 (MQ=255)
                 tCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGatgatg                                                                                                                                      <  2:127580/143‑1 (MQ=255)
                             cAAGGAGCTTGCCGAACTGGGTGT‑CGCCGAGGGGGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTcc                                                                                                                    <  2:149504/149‑1 (MQ=255)
                                gcagcTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGc                                                                                                                 <  4:316409/149‑1 (MQ=255)
                                  agcTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCaa                                                                                                               <  7:32752/149‑1 (MQ=255)
                                    cTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGa                                                                                                                                                    <  5:345810/110‑1 (MQ=255)
                                    cTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGa                                                                                                                                                    >  6:345810/1‑110 (MQ=255)
                                      tGCCGAACAGGGTCTGCGCC‑AGGGCGGTGATCACCAGGCCACGCCCGAGCAGCAGCAGGTAGACAATCAGCAGCACGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGACGGAGTGGCTATCCGCCAAAAAg                                                                                                           >  4:277894/1‑149 (MQ=255)
                                         cGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGTGCTCGGCgag                                                                                                                                          <  2:109142/115‑2 (MQ=255)
                                         cGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGTGCTCGGCgag                                                                                                                                          >  1:109142/1‑114 (MQ=255)
                                         cGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTc                                                                                                         >  5:376104/1‑148 (MQ=255)
                                          gAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTc                                                                                                         >  5:272317/1‑147 (MQ=255)
                                          gAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCa                                                                                                       <  6:272317/149‑1 (MQ=255)
                                                ggTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCg                                                                                                                                <  3:250301/118‑1 (MQ=255)
                                                ggTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCg                                                                                                                                >  4:250301/1‑118 (MQ=255)
                                                  tCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGAc                                                                                               >  4:173410/1‑149 (MQ=255)
                                                   cTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACt                                                                                              >  6:146442/1‑149 (MQ=255)
                                                        cc‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCgatgat                                                                                                                                       >  2:159992/1‑103 (MQ=255)
                                                            gggCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGcc                                                                                       >  8:268520/1‑148 (MQ=255)
                                                             ggCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGcc                                                                                       <  6:376104/147‑1 (MQ=255)
                                                                         cAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCtt                                                                         >  5:142512/1‑149 (MQ=255)
                                                                           ggCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTg                                                                       <  6:142512/149‑1 (MQ=255)
                                                                                           cagcagGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAACCAGCCAGCCTTTGCCAAACACcccgccc                                                        >  5:339822/1‑148 (MQ=255)
                                                                                              cagGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGc                                                    <  1:55777/149‑1 (MQ=255)
                                                                                                ggTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCg                                                  <  7:268520/149‑1 (MQ=255)
                                                                                                 gTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGa                                                 >  4:76692/1‑149 (MQ=255)
                                                                                                       aaTCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATcgcc                                            >  1:263044/1‑148 (MQ=255)
                                                                                                       aaTCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATcgcc                                            <  2:263044/148‑1 (MQ=255)
                                                                                                           agcagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACCGCGATGATGAAGGCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGGCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCt                                        <  5:123175/148‑1 (MQ=255)
                                                                                                            gcatcagGCAGATGCCTAGCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGAGTGGCTTTCCGCCAAAATGTCCAGGTGCGACTGCGTGCGAAGGCCCCAACCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCATTg                                      >  8:349318/1‑149 (MQ=255)
                                                                                                             cagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGGGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCACGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTgg                                     <  5:105223/149‑1 (MQ=255)
                                                                                                              agcagGCAGATGCCTACCAGGCCGAATTCCTCGGCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCGGGCAAAAATTCGAGTTGCGACTGTGTTCGAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGa                                    <  5:265843/149‑1 (MQ=255)
                                                                                                                                 ggCCGAATTCCTCGCCGAGCACGGCGATGATGTTGTCGGTGTGGCTTTCCGTCAAATAGTCGAGGTGCGACTGCGTGCGAAGCAGCCATCCTTTGCCAAACACCCCGCCCGAGGCGATCGCCGCCTTGGACTGGATGATGTTCCAACCg                 <  7:106458/149‑1 (MQ=255)
                                                                                                                                  gCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGCGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCgg                >  8:65575/1‑149 (MQ=255)
                                                                                                                                  gCCGAATTACTCGCCGAGCACGGCGATGATGAATTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTCCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCgg                >  8:70714/1‑149 (MQ=255)
                                                                                                                                   ccGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTTTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGt               <  7:349318/149‑1 (MQ=255)
                                                                                                                                    cGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCaa                                                                   >  4:258715/1‑96 (MQ=255)
                                                                                                                                    cGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCaa                                                                   <  3:258715/96‑1 (MQ=255)
                                                                                                                                         tcatCGCCGTGCTCGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGtt                        <  1:178441/131‑1 (MQ=255)
                                                                                                                                         tcatCGCCGTGCTCGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGtt                        >  2:178441/4‑134 (MQ=255)
                                                                                                                                          catCGCCGTGCTCGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGAGCGCCGCCTTGGACTGGATGATGTTCAAACCGGTGCCCAAc        >  3:214787/3‑149 (MQ=255)
                                                                                                                                           cTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCAACg       >  7:235221/1‑149 (MQ=255)
                                                                                                                                            tCGCCGTGCTCGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACt                                  <  1:261676/121‑1 (MQ=255)
                                                                                                                                            tCGCCGTGCTCGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACt                                  >  2:261676/1‑121 (MQ=255)
                                                                                                                                            tCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGCCTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTGGGACTGGATGATGTTACAACCGGTGCCCAACgg      >  3:216598/1‑149 (MQ=255)
                                                                                                                                            tCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCAACgg      >  7:207476/1‑149 (MQ=255)
                                                                                                                                              gCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCgccgcc                                         >  1:285549/1‑112 (MQ=255)
                                                                                                                                              gCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCgccgcc                                         <  2:285549/112‑1 (MQ=255)
                                                                                                                                                cGAGCCCGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGCTCGCCGCCTTGGACTGAATGATGTTCCAACCGGTGCCCAACGGGTCg  >  4:47297/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TACAAAAAAAGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCC‑TGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCAACGGGTCG  >  NC_002947/5469035‑5469326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑