Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,514,661 A→T 8.3% intergenic (+40/+97) mnaT → / ← cbpM L‑amino acid N‑acyltransferase/chaperone modulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,514,6610AT 8.3% 209.7 / 7.9 72intergenic (+40/+97)mnaT/cbpML‑amino acid N‑acyltransferase/chaperone modulatory protein
Reads supporting (aligned to +/- strand):  ref base A (37/29);  new base T (4/2);  total (41/31)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

CTTCGTGGTGACCGGCCAGATGCCGCAGGTGGGGGTTAAGTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCGGCCCAGCCGCTGCTTCAGCATGCTGTTCT  >  NC_002947/5514516‑5514804
                                                                                                                                                 |                                                                                                                                                 
cTTCGTGGTGACCGGCCAGATGCCGCAGGTGGGGGTTAAGTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAagca                                                                                                                                                <  3:207096/149‑1 (MQ=255)
            cGGACAGATGCCGCAGGTGGGGGTTAAGTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAAtt                                                                                                                                  >  3:102820/1‑149 (MQ=255)
             ggCCAGATACCGCAGGTGGGGGTTAAGTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATTGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAAttt                                                                                                                                 >  3:336592/1‑149 (MQ=255)
                cAGATGCCGCAGGTGGGGGTTAAGTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGATTTGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGc                                                                                                                              <  1:298143/149‑1 (MQ=255)
                cAGATGCCGCAGGTGGGGGTTAAGTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGc                                                                                                                              <  4:188644/149‑1 (MQ=255)
                    tGCCGCCGGTGGGGGTTATGTTTGGCCGCTGGCTTGATCTCACCTACATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTg                                                                                                                          <  8:359426/149‑1 (MQ=255)
                      ccGCAGGTGGGGGTTAAGTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGa                                                                                                                        <  5:342324/149‑1 (MQ=255)
                           ggTGGGGGTTAAGTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCaaa                                                                                                                   <  1:68898/149‑1 (MQ=255)
                            gTGGGGGTTAAGTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCa                                                                                                                     >  6:68417/1‑146 (MQ=255)
                              gggggTTACGTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGTCAATTTTGCGGTGGAGCAAAGAt                                                                                                                <  4:102820/149‑1 (MQ=255)
                                  gTTAAGTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTgg                                                                                                            >  3:299749/1‑149 (MQ=255)
                                       gTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCg                                                                                                                             <  3:271673/127‑1 (MQ=255)
                                       gTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCg                                                                                                                             >  4:271673/1‑127 (MQ=255)
                                       gTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTg                                                                                                       <  4:98631/149‑1 (MQ=255)
                                        tttGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGc                                                                                                      <  4:299749/149‑1 (MQ=255)
                                                   gCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCa                                                                                           >  3:243400/1‑149 (MQ=255)
                                                       gATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTgg                                                                                                            <  8:187842/128‑1 (MQ=255)
                                                       gATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATcgc                                                                                       >  8:141811/1‑149 (MQ=255)
                                                       gATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATAGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTgg                                                                                                            >  7:187842/1‑128 (MQ=255)
                                                            gACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAag                                                                                                  <  4:313432/133‑1 (MQ=255)
                                                            gACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAag                                                                                                  >  3:313432/1‑133 (MQ=255)
                                                              ccTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACaa                                                                                <  2:207019/149‑1 (MQ=255)
                                                               cTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAg                                                                               >  2:49711/1‑149 (MQ=255)
                                                               cTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAg                                                                               <  7:141811/149‑1 (MQ=255)
                                                                   aTGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCg                                                                           >  2:90475/1‑149 (MQ=255)
                                                                     gCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCt                                                                         >  4:94260/1‑149 (MQ=255)
                                                                         cTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCca                                                                     <  4:243400/149‑1 (MQ=255)
                                                                           ggTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCca                                                                     >  3:180489/1‑147 (MQ=255)
                                                                            gTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCcaca                                                                   <  5:68417/148‑1 (MQ=255)
                                                                              gCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGAt                                                                >  7:115457/1‑149 (MQ=255)
                                                                               cTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATg                                                               >  8:243910/1‑149 (MQ=255)
                                                                                tGAACCCGGGCGCCGAACCGCCCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGc                                                              >  3:331979/1‑149 (MQ=255)
                                                                                  aaCCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCg                                                            >  1:218718/1‑149 (MQ=255)
                                                                                  aaCCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCg                                                            >  2:154495/1‑149 (MQ=255)
                                                                                   aCCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCgg                                                           >  2:313453/1‑149 (MQ=255)
                                                                                    cccGGTCGCCTAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGa                                                                                     <  8:361457/122‑1 (MQ=255)
                                                                                    cccGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAagcagc                                                                                              >  3:106212/1‑113 (MQ=255)
                                                                                    cccGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAagcagc                                                                                              <  4:106212/113‑1 (MQ=255)
                                                                                    cccGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGc                                                          >  5:314311/1‑149 (MQ=255)
                                                                                    cccGGGCGCCGAACCGACCTGAAATGAGGTCCATCGTGTGAGCATCGAGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGa                                                                                     >  7:361457/1‑122 (MQ=255)
                                                                                     ccGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGTGGCTGCAAAGCAGCCCATcgc                                                                                       <  4:95584/119‑1 (MQ=255)
                                                                                     ccGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATcgc                                                                                       >  3:95584/1‑119 (MQ=255)
                                                                                         gcgcCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCAcggc                                                     >  4:340951/1‑149 (MQ=255)
                                                                                             cGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCCGTCGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCAcggccg                                                   >  1:298932/1‑149 (MQ=255)
                                                                                             cGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATc                                                                                         <  5:252874/109‑1 (MQ=255)
                                                                                             cGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATc                                                                                         >  6:252874/1‑109 (MQ=255)
                                                                                              gAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACggccggcc                                                <  4:180489/149‑1 (MQ=255)
                                                                                               aaCCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACggccggcc                                                >  3:231082/1‑148 (MQ=255)
                                                                                                 ccGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGAt                                                                >  8:243407/1‑130 (MQ=255)
                                                                                                 ccGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGAt                                                                <  7:243407/130‑1 (MQ=255)
                                                                                                 ccGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTAc                                             <  1:313453/149‑1 (MQ=255)
                                                                                                 ccGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTAc                                             <  2:218718/149‑1 (MQ=255)
                                                                                                  cGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTAc                                             >  8:251641/1‑148 (MQ=255)
                                                                                                           aTGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCTGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATa                                    >  8:342612/1‑148 (MQ=255)
                                                                                                           aTGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATaa                                   <  1:90475/149‑1 (MQ=255)
                                                                                                               ggTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCg                               >  1:216833/1‑149 (MQ=255)
                                                                                                                   cATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCGGcc                            >  2:317063/1‑148 (MQ=255)
                                                                                                                    aTCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCGGCCCa                          <  4:231082/149‑1 (MQ=255)
                                                                                                                       gtgtGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCGGCCCAGc                        <  1:317063/148‑1 (MQ=255)
                                                                                                                          tgAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCGGCCCAGCCgct                    >  2:252840/1‑149 (MQ=255)
                                                                                                                           gAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCGGCCCAGCCgctg                   >  7:221951/1‑149 (MQ=255)
                                                                                                                             gCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCGGCCCAGCCgctgct                 >  4:23643/1‑149 (MQ=255)
                                                                                                                                  gcgAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGTACGGCCGGCCTACAACTGGATAAACCGGCCCAGCCGCTGCTTCAGc            <  7:243910/149‑1 (MQ=255)
                                                                                                                                  gcgAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCGGCCCAGCCGCTGCTTCAGc            <  4:336592/149‑1 (MQ=255)
                                                                                                                                    gccggggCTGCTTTGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACt                                         >  3:304278/5‑118 (MQ=38)
                                                                                                                                    gccggggCTGCTTTGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACt                                         <  4:304278/114‑1 (MQ=38)
                                                                                                                                    gccggggCTGCTTTGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACt                                         <  4:309423/114‑1 (MQ=38)
                                                                                                                                    gccggggCTGCTTTGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACt                                         >  3:309423/5‑118 (MQ=38)
                                                                                                                                    gccggggCTGCTTTGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCGGCCCAGCCGCTGCTTCAGCAt          >  2:356536/5‑149 (MQ=255)
                                                                                                                                    gccggggCTGCTTTGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCGGCCCAGCCGCTGCTTCAGCAt          >  2:310288/5‑149 (MQ=255)
                                                                                                                                     aaaGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCGGCCCAGCCGCTGCTTCAGCATg         <  6:314311/149‑1 (MQ=255)
                                                                                                                                          gCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCGGCCCAGCCGCTGCTTCAGCATGCTGt     >  7:138839/1‑148 (MQ=255)
                                                                                                                                            tGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCGGCCAAGCCGCTGCTTCAGCATGCTGTtct  >  4:283326/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CTTCGTGGTGACCGGCCAGATGCCGCAGGTGGGGGTTAAGTTTGGCCGCTGGCTTGATCTGACCTTCATGCAACTGGTGCTGAACCCGGGCGCCGAACCGACCTGAAATGCGGTCCATCGTGTGAGCATCGCGAAAGGGCTGCAAAGCAGCCC‑‑CGGCAATTTTGCGGTGGAGCAAAGATCTGGGGCTGCAAAGCAGCCCATCGCGACACAAGGCCGCTCCCACAGATGCCGGCACGGCCGGCCTACAACTGGATAAACCGGCCCAGCCGCTGCTTCAGCATGCTGTTCT  >  NC_002947/5514516‑5514804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: