| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,586,523 | T→C | 6.2% | T255A (ACC→GCC) | PP_4914 ← | phytase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,586,523 | 0 | T | C | 6.2% | 189.8 / 2.1 | 64 | T255A (ACC→GCC) | PP_4914 | phytase‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (28/32); new base C (2/2); total (30/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
ATCGATCACCAGTGCTTCGGCCAGGCCAAACGGCTGCGCGTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTCAGCAGCACGCAACCACGCCCGCAGGTCCAGACCGACTGCTGGCGCT > NC_002947/5586377‑5586669 | aTCGATCACCAGTGCTTCGGCCAGGCCAAACGGCTGCGCGTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTaa < 7:287091/149‑1 (MQ=255)aTCGATCACCAGTGCTTCGGCCAGGCCAAACGGCTGCGCGTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTa > 1:24488/1‑148 (MQ=255)aTCGATCACCAGTGCTGCGGCCAGGCCAAACGGCTGCGCGTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTaa < 8:314316/149‑1 (MQ=255) gATCACCAGTGCTTCGGCCAGGCCAAACGGCTGCGCGTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAAc < 2:24488/148‑1 (MQ=255) cGGCCAGGCCAAACGGCTGCGCGTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCTACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGCAAGCTGTTTACCCTCGaa < 8:247016/148‑1 (MQ=255) ccaggccaAACGGCTGCGCGTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAAccag < 7:121995/149‑1 (MQ=255) aggccaAACGGCTGCGCGTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAAccaggc < 4:361764/149‑1 (MQ=255) aggccaAACGGCTGCGCGTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAAccaggc < 3:179190/149‑1 (MQ=255) ccaAACGGCTGCGCGTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAAccaggccag < 3:50647/149‑1 (MQ=255) cTGCGCGTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGa < 7:306747/149‑1 (MQ=255) gcgcGTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGCAAGCTGTTTACCCTCGAAc < 6:197597/131‑1 (MQ=255) gcgcGTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGCAAGCTGTTTACCCTCGAAc > 5:197597/1‑131 (MQ=255) cgTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTcg > 7:49853/1‑149 (MQ=255) cgTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTcg > 5:242266/1‑149 (MQ=255) gcgcCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGaa < 6:230943/141‑1 (MQ=255) gcgcCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGaa > 5:230943/1‑141 (MQ=255) gcCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGa > 2:206033/1‑148 (MQ=255) gTGACTCGACCAGCGCATCGGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAgg > 2:366614/1‑149 (MQ=255) cTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTc > 3:254880/1‑111 (MQ=255) cTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTc < 4:254880/111‑1 (MQ=255) cAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGaa < 8:257229/108‑1 (MQ=255) cAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGaa > 7:257229/1‑108 (MQ=255) aGCACATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCAtt < 3:107815/149‑1 (MQ=255) cgcATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTgg < 6:242266/149‑1 (MQ=255) gcATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTggg < 7:160120/149‑1 (MQ=255) aTCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCCATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACCGGGCCCTGGCATTGGGCa > 1:362802/1‑149 (MQ=255) cagcagCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATc > 6:23459/1‑149 (MQ=255) cagcagCAAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATc > 8:316397/1‑149 (MQ=255) cGACCAGCAACGCTCGACCTTGCCACTGTGGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGtt < 5:47633/149‑1 (MQ=255) cAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTggg < 1:35952/131‑1 (MQ=255) cAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTggg > 2:35952/1‑131 (MQ=255) aGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTg > 6:286193/1‑145 (MQ=255) aGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTg < 5:286193/145‑1 (MQ=255) gCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCAttt > 6:54106/1‑149 (MQ=255) aCGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGcgcg < 6:196961/136‑1 (MQ=255) aCGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGcgcg > 5:196961/1‑136 (MQ=255) tCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGtgcg < 7:147089/132‑1 (MQ=255) tCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGtgcg > 8:147089/1‑132 (MQ=255) tGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCg > 3:102834/1‑149 (MQ=255) ccACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGc < 1:206033/148‑1 (MQ=255) aCTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTca < 4:102834/149‑1 (MQ=255) tGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGcc > 5:377482/1‑98 (MQ=255) tGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGcc < 6:377482/98‑1 (MQ=255) tGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTcagc < 7:316397/149‑1 (MQ=255) tatCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTCAGCAGCACg > 3:14842/1‑149 (MQ=255) cACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTcagc > 1:197750/1‑140 (MQ=255) cACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTcagc < 2:197750/140‑1 (MQ=255) aCGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTCAGCAGCACGCaa < 4:356790/148‑1 (MQ=255) aCGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTCAGCAGCACGCaa > 3:356790/1‑148 (MQ=255) aCGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTCAGCAGCACGCAAc < 1:366614/149‑1 (MQ=255) gCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGcc > 8:292996/1‑82 (MQ=255) gCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGcc < 7:292996/82‑1 (MQ=255) gCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTca > 6:114176/1‑131 (MQ=255) gCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTca < 5:114176/131‑1 (MQ=255) gCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTCAGCAGCACGCAACCACGcc > 8:9322/1‑149 (MQ=255) gCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTCAGCAGCACGCAACCACGcc > 8:3765/1‑149 (MQ=255) ggCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTCAGCAGCACGCAACCACGCCCGCAGGTCCAg < 8:49853/148‑1 (MQ=255) gCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTCAGCAGCACGCAACCACGCCCGCAGGTCCAGAc < 5:23459/149‑1 (MQ=255) cGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTgcg < 3:51982/87‑1 (MQ=255) cGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTgcg > 4:51982/1‑87 (MQ=255) cGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTCAGCAGCACGCAACCACGCCCGCAGGTCCAGACCGAct < 5:54106/148‑1 (MQ=255) tCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTCAGCAGCACGCAACCACGCCCGCAGGTCCAGACCGActgctg > 1:241091/1‑149 (MQ=255) cGAGGGCAAGCTGTTTACCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTAAGCAGCACGCAACCACGCCCGCAGGTCCAGACCGACTGCTgg > 2:331921/1‑149 (MQ=255) ggTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTCAGCAGCACGCAACCACGCCCGCAGGTCCAGACCGACTGCTGGCGCt > 5:224629/1‑149 (MQ=255) | ATCGATCACCAGTGCTTCGGCCAGGCCAAACGGCTGCGCGTAGCGCCGTGACTCGACCAGCGCATCAGCAGCGAACGACCAGCAACGCTCGACCTTGCCACTGTCGATATCACGTCGGCACACCCGGTAGGCGTTACGTTCGAGGGTAAACAGCTTGCCCTCGAACCAGGCCAGGTCAGCGAAGTCGCGCGACAGGGCCCTGGCATTGGGCATCTGCGCGGGTTGCATTTCGACCCCCGCCTCGCTCAGCAGCACGCAACCACGCCCGCAGGTCCAGACCGACTGCTGGCGCT > NC_002947/5586377‑5586669 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |