Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,641,460 C→G 9.1% Q375E (CAG→GAG)  PP_4951 → EmrB/QacA family drug resistance transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,641,4600CG 9.1% 158.0 / 6.9 55Q375E (CAG→GAG) PP_4951EmrB/QacA family drug resistance transporter
Reads supporting (aligned to +/- strand):  ref base C (24/26);  new base G (3/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01

ATCGAACGGTTCGGCTATCGCATCATCCTCACCGGTAATACCTTGCTGTTGGGCATCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTGGGTGTGGCCTGCGCCGGCGCACTGTTGGGCGGCTTTACTGCGGC  >  NC_002947/5641313‑5641608
                                                                                                                                                   |                                                                                                                                                    
aTCGAACGGTTCGGCTATCGCATCATCCTCACCGGTAATACCTTGCTGTTGGGCATCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCa                                                                                                                                                     <  1:26838/149‑1 (MQ=255)
    aaCGGTTCGGCTATCGCATCATCCTCACCGGTAATACCTTGCTGTTGGGCATCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGc                                                                                                                                                      >  7:151192/1‑144 (MQ=255)
    aaCGGTTCGGCTATCGCATCATCCTCACCGGTAATACCTTGCTGTTGGGCATCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGc                                                                                                                                                      <  8:151192/144‑1 (MQ=255)
    aaCGGTTCGGCTATCGCATCATCCTCACCGGTAATACCTTGCTGTTGGGCATCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGtt                                                                                                                                                  >  5:97000/1‑148 (MQ=255)
             tcTATCGCATCATACTCACCGGTAATACCTTGCTGTTGGGGTTCCTGCTGTGAATGCTGGGCCTGGGGGACGAGCAGTCCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGATCTCCATGCAGTTCACTGCGATg                                                                                                                                        <  7:210042/148‑1 (MQ=255)
                 tCGCATCATCCTCACCGGTAATACCTTGCTGTTGGGCATCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGaa                                                                                                                                      <  8:34363/147‑1 (MQ=255)
                 tCGCATCATCCTCACCGGTAATACCTTGCTGTTGGGCATCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAaca                                                                                                                                    <  8:225650/149‑1 (MQ=255)
                     atcatcCTCACCGGTAATACCTTGCTGTTGGGCATCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGt                                                                                                                                <  6:369460/149‑1 (MQ=255)
                        atcCTCACCGGTAATACCTTGCTGTTGGGCATCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGAc                                                                                                                             >  6:38327/1‑149 (MQ=255)
                            tCACCGGTAATACCTTGCTGTTGGGCATCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCt                                                                                                                          >  2:138531/1‑148 (MQ=255)
                                 ggTAATGCCTTGCTGTTGGGCATCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGa                                                                                                                    >  6:336065/1‑149 (MQ=255)
                                     aTACCTTGCTGTTGGGCATCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACt                                                                                                                                              >  1:149748/1‑119 (MQ=255)
                                            gCTGTTGGGCATCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTcgacga                                                                                                           <  2:211582/147‑1 (MQ=255)
                                                       tCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGa                                                                                                                              >  6:370628/1‑117 (MQ=255)
                                                       tCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGa                                                                                                                              <  5:370628/117‑1 (MQ=255)
                                                       tCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTcg                                                                                                               <  4:304770/132‑1 (MQ=255)
                                                       tCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGATCATCGATCTcg                                                                                                               >  3:304770/1‑132 (MQ=255)
                                                          tgctgGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCagcagc                                                                                           >  5:324469/1‑149 (MQ=255)
                                                          tgctgGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCagcagc                                                                                           >  8:200097/1‑149 (MQ=255)
                                                            ctgGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCgg                                                                                         <  5:38327/149‑1 (MQ=255)
                                                               gCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGt                                                                                        <  5:336065/147‑1 (MQ=255)
                                                               gCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGt                                                                                        <  7:267341/147‑1 (MQ=255)
                                                                cAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAAc                                                                                     <  7:200097/149‑1 (MQ=255)
                                                                 acgcctgggacaggTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTTAACTCCATTCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGAcgccagcgcca                                                                                                <  6:266975/135‑1 (MQ=255)
                                                                 aaGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGAcgccagcgcca                                                                                                <  6:271763/137‑1 (MQ=255)
                                                                 aaGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGAcgccagcgcca                                                                                                >  5:266975/1‑137 (MQ=255)
                                                                 aaGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGAcgccagcgcca                                                                                                >  5:271763/1‑137 (MQ=255)
                                                                 aaGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTa                                                                                       <  4:177169/146‑1 (MQ=255)
                                                                 aaGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTa                                                                                       >  3:177169/1‑146 (MQ=255)
                                                                     ctgggcctggTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCct                                                                                <  6:324469/149‑1 (MQ=255)
                                                                       gggcctggTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCctgc                                                                              >  2:17017/1‑149 (MQ=255)
                                                                              gtggACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCg                                                                         <  1:138531/147‑1 (MQ=255)
                                                                                      gCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGt                                                                                        <  1:340280/124‑1 (MQ=255)
                                                                                      gCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGc                                                                                  >  3:195695/1‑130 (MQ=255)
                                                                                          aCCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACt                                                           <  1:17017/149‑1 (MQ=255)
                                                                                            cccTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGt                                                         >  8:15256/1‑149 (MQ=255)
                                                                                               ttATTGGCTGCTGTTTGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCctgc                                                                              <  4:280142/125‑1 (MQ=255)
                                                                                                  ttGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCt                                                     >  2:358791/1‑147 (MQ=255)
                                                                                                     gctgctGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGt                                                                                        >  2:316322/1‑109 (MQ=255)
                                                                                                     gctgctGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGcc                                                >  4:166234/1‑149 (MQ=255)
                                                                                                           gTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGAcgccagcgcca                                                                                                >  4:33641/1‑95 (MQ=255)
                                                                                                           gTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGAcgccagcgcca                                                                                                <  3:33641/95‑1 (MQ=255)
                                                                                                            ttGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTGGgt                                           <  7:271600/147‑1 (MQ=255)
                                                                                                                tGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTGGGTGTGGcc                                     >  2:128689/1‑149 (MQ=255)
                                                                                                                            ttCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTGGGTGTgg                                       >  6:11198/1‑135 (MQ=255)
                                                                                                                            ttCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTGGGTGTgg                                       <  5:11198/135‑1 (MQ=255)
                                                                                                                            ttCTGGGCGCAGTGAACTCCATGCAGTTAACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCTCGCAACTGTAGCTGAGCCTGGGTGTGGCCTGAGCCGgcgc                          >  8:347607/1‑148 (MQ=255)
                                                                                                                                  tcgcAGTGAACTGCATGGAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTgg                                             >  1:18326/2‑123 (MQ=255)
                                                                                                                                  tcgcAGTGAACTGCATGGAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTgg                                             <  2:18326/122‑1 (MQ=255)
                                                                                                                                   cgcAGTGAACTGCATGGAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTGGGTGTGGCCTGCGCCGGCGCACTGTTgg                  >  4:96307/1‑149 (MQ=255)
                                                                                                                                   cgcAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTGGGTGTGGCCTGCGCCGGCGCACTGTTgg                  <  1:128689/149‑1 (MQ=255)
                                                                                                                                    gcAGTGAACTGCATGGAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTGGGTGTGGCCTGCGCCGGCGCACTGTTggg                 >  8:135545/1‑149 (MQ=255)
                                                                                                                                        tGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTGGGTGTGGCCTGCGCCGGCGCACTGTTGggcggc             <  3:166234/149‑1 (MQ=255)
                                                                                                                                          aaCTGCATGGAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTGGGTGTGGCCTGCGCCGGCGCACTGTTGGGCGGCt            <  7:135545/148‑1 (MQ=255)
                                                                                                                                           aCTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGAcgccagcgcca                                                                                                <  6:43654/63‑1 (MQ=255)
                                                                                                                                           aCTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGAcgccagcgcca                                                                                                >  5:43654/1‑63 (MQ=255)
                                                                                                                                                aTGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTGGGTGTGGCCTGCGCCGGCGCACTGTTGGGCGGCTTTACTGc     >  2:188037/1‑149 (MQ=255)
                                                                                                                                                   cAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTGGGTGTGGCCTGCGCCGGCGCACTGTTGGGCGGCTTTACTGCggc  <  1:188037/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATCGAACGGTTCGGCTATCGCATCATCCTCACCGGTAATACCTTGCTGTTGGGCATCCTGCTGGCAAGCCTGGGCCTGGTGGACGAGCAGACCCCTTATTGGCTGCTGTTGGTGCAACTTGGCCTTCTGGGCGCAGTGAACTCCATGCAGTTCACTGCGATGAACACAGTGACGCTCATCGATCTCGACGACGCCAGCGCCAGCAGCGGTAACAGCCTGCTGTCGGTGGTCGCGCAACTGTCGCTGAGCCTGGGTGTGGCCTGCGCCGGCGCACTGTTGGGCGGCTTTACTGCGGC  >  NC_002947/5641313‑5641608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑