Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,645,313	G→C	6.5%	L222L (CTG→CTC) ‡	PP_4954 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,645,313	0	G	C	6.5%	244.1 / 6.4	77	L222L (CTG→CTC) ‡	PP_4954	membrane protein
Reads supporting (aligned to +/- strand):  ref base G (34/38);  new base C (3/2);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

GGTACAGCAGAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACGCACTGACCGA  >  NC_002947/5645166‑5645459                                                                                                                                                    |                                                                                                                                                    ggTACAGCAGAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGa                                                                                                                                                    <  6:211911/149‑1 (MQ=255)       gcagAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAg                                                                                                                                              >  6:235824/1‑149 (MQ=255)        cagAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGc                                                                                                                                             >  1:15698/1‑149 (MQ=255)         agagCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGc                                                                                                                                             >  3:89293/1‑148 (MQ=255)                     gACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGat‑catc                                                                                                                               <  1:368755/149‑1 (MQ=255)                               cGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCggg                                                                                                                     >  4:24917/1‑149 (MQ=255)                                 aGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCTAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTg                                                                                                                   <  2:231393/149‑1 (MQ=255)                                      ggCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGCT‑CATCCTGCACCGGGTGGGCaa                                                                                                              >  7:228837/1‑149 (MQ=255)                                      ggCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGc                                                                                                                <  1:42501/147‑1 (MQ=255)                                       gCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTgg                                                                                                                  <  7:297353/144‑1 (MQ=255)                                       gCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTgg                                                                                                                  >  8:297353/1‑144 (MQ=255)                                         aGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAg                                                                                                           <  3:24917/149‑1 (MQ=255)                                              ccTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGccaa                                                                                                       <  8:182973/148‑1 (MQ=255)                                              ccTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGccaa                                                                                                       >  7:182973/1‑148 (MQ=255)                                                 gAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTgg                                                                                                                  <  4:226748/134‑1 (MQ=255)                                                 gAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTgg                                                                                                                  >  3:226748/1‑134 (MQ=255)                                                    gCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACt                                                                                                >  5:91198/1‑149 (MQ=255)                                                      tCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTc                                                                                               >  1:260435/1‑148 (MQ=255)                                                          ggCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAAc                                                                                                             <  1:318095/130‑1 (MQ=255)                                                          ggCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAAc                                                                                                             >  2:318095/1‑130 (MQ=255)                                                          ggCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGGAgg                                                                                          >  3:116677/1‑149 (MQ=255)                                                             cAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAg                                                                                                                                              <  1:174604/95‑1 (MQ=255)                                                             cAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAg                                                                                                                                              >  2:174604/1‑95 (MQ=255)                                                             cAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGGAGGTaa                                                                                       <  4:116677/149‑1 (MQ=255)                                                             cAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGt                                                                                         >  3:107633/1‑147 (MQ=255)                                                             cAGCCTGTTTGAACCGGGCCGCAAGCTCGACGTCGAGGCACTGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAATGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTaa                                                                                       <  5:256106/149‑1 (MQ=255)                                                               gCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGc                                                                                     <  6:91198/149‑1 (MQ=255)                                                                ccTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGcc                                                                                    <  4:169908/149‑1 (MQ=255)                                                                ccTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGcc                                                                                    <  2:260435/149‑1 (MQ=255)                                                                    tttGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTa                                                                                >  3:216163/1‑149 (MQ=255)                                                                     ttGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTAc                                                                               >  7:198562/1‑149 (MQ=255)                                                                      tGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTa                                                                                <  4:78791/147‑1 (MQ=255)                                                                      tGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTa                                                                                >  3:78791/1‑147 (MQ=255)                                                                      tGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑AATCCTGCACAGGGTGCGCAACAGCCCACCAAACTCGAAGGTAAGCCGCTAc                                                                               >  2:363851/1‑148 (MQ=255)                                                                       gAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTAcc                                                                              <  5:235824/148‑1 (MQ=255)                                                                           cGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAg                                                                         >  2:232227/1‑149 (MQ=255)                                                                           cGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAg                                                                         <  4:216163/149‑1 (MQ=255)                                                                                cGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGt                                                                     <  1:379564/148‑1 (MQ=255)                                                                                       tCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGt                                                                                                                    <  5:332020/94‑1 (MQ=255)                                                                                       tCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGt                                                                                                                    >  6:332020/1‑94 (MQ=255)                                                                                             tCGAGGCACGGCGCCTGGAACTGGCCCAGCATAACGGCAAGGTGGTGCCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTTGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAg                                                       <  8:261315/149‑1 (MQ=255)                                                                                              cGAGGCACTGCGCCTGTAACTTGCCCAGCAGAACGGCAAGGTGGTGTCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAg                                                       <  8:266410/148‑1 (MQ=255)                                                                                               gAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAg                                                                                                           >  5:167623/1‑95 (MQ=255)                                                                                               gAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAgg                                                      <  3:167595/148‑1 (MQ=255)                                                                                               gAGGCACGGCGACTGGAACTGGCCCAGCAGAACTGCAAGGTGGTGGACGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAg                                                                                                           <  6:167623/95‑1 (MQ=255)                                                                                                aGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGat                                                    <  2:15698/149‑1 (MQ=255)                                                                                                    aCGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATcc                                                <  1:38201/149‑1 (MQ=255)                                                                                                    aCGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATc                                                 >  3:93842/1‑148 (MQ=255)                                                                                                         gcCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAg                                           >  3:65129/1‑149 (MQ=255)                                                                                                             ggAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAg                                                                         <  1:220502/115‑1 (MQ=255)                                                                                                              gAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCgt                                        <  4:93842/147‑1 (MQ=255)                                                                                                              gAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCgt                                        <  3:137150/147‑1 (MQ=255)                                                                                                                    gCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAATGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTg                                 <  1:363851/148‑1 (MQ=255)                                                                                                                      ccAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGc                                          <  6:7573/137‑1 (MQ=255)                                                                                                                      ccAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGc                                          >  5:7573/1‑137 (MQ=255)                                                                                                                      ccAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCt                              >  2:312623/1‑149 (MQ=255)                                                                                                                        agcagAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCg                            >  2:333596/1‑149 (MQ=255)                                                                                                                          cagAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCa                          <  4:107633/149‑1 (MQ=255)                                                                                                                           agAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCAc                         >  5:284525/1‑149 (MQ=255)                                                                                                                                ggCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTAccc                    <  8:228837/149‑1 (MQ=255)                                                                                                                                  cAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGc                                          <  4:155740/125‑1 (MQ=255)                                                                                                                                  cAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGc                                          >  3:155740/1‑125 (MQ=255)                                                                                                                                  cAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTAcccc                   >  4:253779/1‑148 (MQ=255)                                                                                                                                      gtggtgGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACaa              >  5:256054/1‑149 (MQ=255)                                                                                                                                       tggtggCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAAc             <  8:198562/149‑1 (MQ=255)                                                                                                                                       ccttggCTGCGTTCAGCGCGGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAAc             >  1:39673/4‑149 (MQ=255)                                                                                                                                         ttggCTGCGTTCAGCGCGGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTAccc                    >  7:99996/2‑140 (MQ=255)                                                                                                                                         ttggCTGCGTTCAGCGCGGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTAccc                    <  8:99996/139‑1 (MQ=255)                                                                                                                                         ttggCTGCGTTCAGCGCGGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAAc             >  7:54876/2‑147 (MQ=255)                                                                                                                                         ttggCTGCGTTCAGCGCGGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTCTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAAc             <  8:54876/146‑1 (MQ=255)                                                                                                                                         gtggCCGCGCTGAACGCAGCCAAGGAGATGCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACg            >  1:28958/1‑149 (MQ=255)                                                                                                                                         gtggCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTAc                      <  2:81777/138‑1 (MQ=255)                                                                                                                                         gtggCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTAc                      >  1:81777/1‑138 (MQ=255)                                                                                                                                              cgcgCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCa                                               >  6:104679/1‑108 (MQ=255)                                                                                                                                              cgcgCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCa                                               <  5:104679/108‑1 (MQ=255)                                                                                                                                              cgcgCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACGCACTGa      >  8:253375/1‑149 (MQ=255)                                                                                                                                                  cTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACGCACTGACCGa  <  1:333596/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GGTACAGCAGAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACGCACTGACCGA  >  NC_002947/5645166‑5645459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑