Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,645,315	A→G	6.6%	N223S (AAC→AGC)	PP_4954 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,645,315	0	A	G	6.6%	224.2 / 4.1	76	N223S (AAC→AGC)	PP_4954	membrane protein
Reads supporting (aligned to +/- strand):  ref base A (34/37);  new base G (3/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

GCAGAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACGCACTGACCGAG  >  NC_002947/5645172‑5645460                                                                                                                                                |                                                                                                                                                   gcagAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAg                                                                                                                                               >  6:235824/1‑149 (MQ=255)  cagAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGc                                                                                                                                              >  1:15698/1‑149 (MQ=255)   agagCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGc                                                                                                                                              >  3:89293/1‑148 (MQ=255)               gACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGat‑catc                                                                                                                                <  1:368755/149‑1 (MQ=255)                         cGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCggg                                                                                                                      >  4:24917/1‑149 (MQ=255)                           aGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCTAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTg                                                                                                                    <  2:231393/149‑1 (MQ=255)                                ggCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGCT‑CATCCTGCACCGGGTGGGCaa                                                                                                               >  7:228837/1‑149 (MQ=255)                                ggCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGc                                                                                                                 <  1:42501/147‑1 (MQ=255)                                 gCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTgg                                                                                                                   >  8:297353/1‑144 (MQ=255)                                 gCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTgg                                                                                                                   <  7:297353/144‑1 (MQ=255)                                   aGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAg                                                                                                            <  3:24917/149‑1 (MQ=255)                                        ccTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGccaa                                                                                                        <  8:182973/148‑1 (MQ=255)                                        ccTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGccaa                                                                                                        >  7:182973/1‑148 (MQ=255)                                           gAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTgg                                                                                                                   >  3:226748/1‑134 (MQ=255)                                           gAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTgg                                                                                                                   <  4:226748/134‑1 (MQ=255)                                              gCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACt                                                                                                 >  5:91198/1‑149 (MQ=255)                                                tCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTc                                                                                                >  1:260435/1‑148 (MQ=255)                                                    ggCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAAc                                                                                                              >  2:318095/1‑130 (MQ=255)                                                    ggCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAAc                                                                                                              <  1:318095/130‑1 (MQ=255)                                                    ggCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGGAgg                                                                                           >  3:116677/1‑149 (MQ=255)                                                       cAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAg                                                                                                                                               >  2:174604/1‑95 (MQ=255)                                                       cAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAg                                                                                                                                               <  1:174604/95‑1 (MQ=255)                                                       cAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGGAGGTaa                                                                                        <  4:116677/149‑1 (MQ=255)                                                       cAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGt                                                                                          >  3:107633/1‑147 (MQ=255)                                                       cAGCCTGTTTGAACCGGGCCGCAAGCTCGACGTCGAGGCACTGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAATGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTaa                                                                                        <  5:256106/149‑1 (MQ=255)                                                         gCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGc                                                                                      <  6:91198/149‑1 (MQ=255)                                                          ccTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGcc                                                                                     <  4:169908/149‑1 (MQ=255)                                                          ccTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGcc                                                                                     <  2:260435/149‑1 (MQ=255)                                                              tttGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTa                                                                                 >  3:216163/1‑149 (MQ=255)                                                               ttGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTAc                                                                                >  7:198562/1‑149 (MQ=255)                                                                tGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTa                                                                                 >  3:78791/1‑147 (MQ=255)                                                                tGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTa                                                                                 <  4:78791/147‑1 (MQ=255)                                                                tGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑AATCCTGCACAGGGTGCGCAACAGCCCACCAAACTCGAAGGTAAGCCGCTAc                                                                                >  2:363851/1‑148 (MQ=255)                                                                 gAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTAcc                                                                               <  5:235824/148‑1 (MQ=255)                                                                     cGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAg                                                                          >  2:232227/1‑149 (MQ=255)                                                                     cGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAg                                                                          <  4:216163/149‑1 (MQ=255)                                                                          cGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGt                                                                      <  1:379564/148‑1 (MQ=255)                                                                                 tCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGt                                                                                                                     >  6:332020/1‑94 (MQ=255)                                                                                 tCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGt                                                                                                                     <  5:332020/94‑1 (MQ=255)                                                                                       tCGAGGCACGGCGCCTGGAACTGGCCCAGCATAACGGCAAGGTGGTGCCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTTGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAg                                                        <  8:261315/149‑1 (MQ=255)                                                                                        cGAGGCACTGCGCCTGTAACTTGCCCAGCAGAACGGCAAGGTGGTGTCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAg                                                        <  8:266410/148‑1 (MQ=255)                                                                                         gAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAg                                                                                                            >  5:167623/1‑95 (MQ=255)                                                                                         gAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAgg                                                       <  3:167595/148‑1 (MQ=255)                                                                                         gAGGCACGGCGACTGGAACTGGCCCAGCAGAACTGCAAGGTGGTGGACGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAg                                                                                                            <  6:167623/95‑1 (MQ=255)                                                                                          aGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGat                                                     <  2:15698/149‑1 (MQ=255)                                                                                              aCGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATcc                                                 <  1:38201/149‑1 (MQ=255)                                                                                              aCGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATc                                                  >  3:93842/1‑148 (MQ=255)                                                                                                   gcCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAg                                            >  3:65129/1‑149 (MQ=255)                                                                                                       ggAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAg                                                                          <  1:220502/115‑1 (MQ=255)                                                                                                        gAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCgt                                         <  4:93842/147‑1 (MQ=255)                                                                                                        gAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCgt                                         <  3:137150/147‑1 (MQ=255)                                                                                                              gCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAATGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTg                                  <  1:363851/148‑1 (MQ=255)                                                                                                                ccAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGc                                           <  6:7573/137‑1 (MQ=255)                                                                                                                ccAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGc                                           >  5:7573/1‑137 (MQ=255)                                                                                                                ccAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCt                               >  2:312623/1‑149 (MQ=255)                                                                                                                  agcagAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCg                             >  2:333596/1‑149 (MQ=255)                                                                                                                    cagAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCa                           <  4:107633/149‑1 (MQ=255)                                                                                                                     agAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCAc                          >  5:284525/1‑149 (MQ=255)                                                                                                                          ggCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTAccc                     <  8:228837/149‑1 (MQ=255)                                                                                                                            cAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGc                                           <  4:155740/125‑1 (MQ=255)                                                                                                                            cAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGc                                           >  3:155740/1‑125 (MQ=255)                                                                                                                            cAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTAcccc                    >  4:253779/1‑148 (MQ=255)                                                                                                                                gtggtgGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACaa               >  5:256054/1‑149 (MQ=255)                                                                                                                                 tggtggCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAAc              <  8:198562/149‑1 (MQ=255)                                                                                                                                 ccttggCTGCGTTCAGCGCGGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAAc              >  1:39673/4‑149 (MQ=255)                                                                                                                                   ttggCTGCGTTCAGCGCGGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTAccc                     <  8:99996/139‑1 (MQ=255)                                                                                                                                   ttggCTGCGTTCAGCGCGGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTAccc                     >  7:99996/2‑140 (MQ=255)                                                                                                                                   ttggCTGCGTTCAGCGCGGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAAc              >  7:54876/2‑147 (MQ=255)                                                                                                                                   ttggCTGCGTTCAGCGCGGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTCTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAAc              <  8:54876/146‑1 (MQ=255)                                                                                                                                   gtggCCGCGCTGAACGCAGCCAAGGAGATGCATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACg             >  1:28958/1‑149 (MQ=255)                                                                                                                                   gtggCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTAc                       >  1:81777/1‑138 (MQ=255)                                                                                                                                   gtggCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTAc                       <  2:81777/138‑1 (MQ=255)                                                                                                                                        cgcgCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCa                                                <  5:104679/108‑1 (MQ=255)                                                                                                                                        cgcgCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCa                                                >  6:104679/1‑108 (MQ=255)                                                                                                                                        cgcgCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACGCACTGa       >  8:253375/1‑149 (MQ=255)                                                                                                                                            cTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACGCACTGACCGa   <  1:333596/149‑1 (MQ=255)                                                                                                                                               aaCGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAg                                                        >  2:141246/1‑93 (MQ=255)                                                                                                                                               aaCGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAg                                                        <  1:141246/93‑1 (MQ=255)                                                                                                                                               aaCGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACGCACTGACCGAg  <  2:28958/147‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   GCAGAGCCTGGCCAGACTGTTCTTCGAGCTGGGCAGCTACCTGAAGCTCAAGGCCAGCCTGTTTGAACCGGTCCGCACGCTCGACGTCGAGGCACGGCGCCTGGAACTGGCCCAGCAGAACGGCAAGGTGGTGGCCGCGCTGAACGCAGCCAAGGAGAT‑CATCCTGCACCGGGTGGGCAACAGCCAACCAAACTCGAAGGTAAGCCGCTACCTCAAGCTGTACTTCCTGGCCCAGGATATCCACGAGCGTGTCAGTGCCTCGCACTACCCCTACAACGCACTGACCGAG  >  NC_002947/5645172‑5645460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑