Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,650,792 T→A 5.5% D134V (GAC→GTC)  PP_4959 ← diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,650,7920TA 5.5% 219.6 / 2.3 73D134V (GAC→GTC) PP_4959diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (34/35);  new base A (2/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01

AATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCGGG  >  NC_002947/5650644‑5650936
                                                                                                                                                    |                                                                                                                                                
aaTGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGt                                                                                                                                                  <  4:138408/149‑1 (MQ=255)
     ccGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGag                                                                                                                                              >  3:304298/1‑148 (MQ=255)
         aTTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTCGACCAGCAgcg                                                                                                                                         <  6:81803/149‑1 (MQ=255)
             gCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTg                                                                                                                                     >  2:77172/1‑149 (MQ=255)
             gCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTg                                                                                                                                     <  1:203261/149‑1 (MQ=255)
              ctaatcGGTTGTTGTCATTGGCATCATGCACCAGATTGGGTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTGACGACTCAGCTGCTGCAGCATGCGCCGGTTGGGGTGCTGTTGGAGCAGCGCCTgg                                                                                                                                    <  2:286831/144‑1 (MQ=255)
               aaaaCGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGa                                                                                                                                   >  2:122349/1‑149 (MQ=255)
               aaaaCGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGa                                                                                                                                   >  1:36931/1‑149 (MQ=255)
                 aaCGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCAGCAGCATGCGCCGCTTGCGCTGCTGGTCGAGCAGCGCCTGGAcc                                                                                                                                 >  8:151544/1‑149 (MQ=255)
                    ggttgatgCCCATGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTg                                                                                                                              >  8:126387/4‑149 (MQ=255)
                     gatgatGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTgg                                                                                                                             >  2:120030/1‑149 (MQ=255)
                      atgatgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTggg                                                                                                                            <  6:348520/149‑1 (MQ=255)
                       tgatgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGc                                                                                                                           <  1:350990/149‑1 (MQ=255)
                       tgatgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGc                                                                                                                           <  4:44005/149‑1 (MQ=255)
                         atgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCtt                                                                                                                         <  3:375458/149‑1 (MQ=255)
                           gCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCtt                                                                                                                         >  7:71491/1‑147 (MQ=255)
                            cccatggCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                       <  7:126387/143‑1 (MQ=255)
                            ccATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                       <  6:20395/148‑1 (MQ=255)
                            ccATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGAGCCGGTTGCGCTGCTGGTCCAGCAGCGCCTGGACCTTGGGCGTGAg                                                                                                                      >  6:83681/1‑149 (MQ=255)
                             cATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAgg                                                                                                                     <  6:390690/149‑1 (MQ=255)
                                 ggCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGAttt                                                                                                                 <  7:15556/149‑1 (MQ=255)
                                  gCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTtg                                                                                                                >  3:187299/1‑149 (MQ=255)
                                   cATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCAGGACTCAGCTGCTGCAGCTTGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTtgt                                                                                                               <  7:151544/149‑1 (MQ=255)
                                   cATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTtgt                                                                                                               <  6:282702/149‑1 (MQ=255)
                                   cATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTtgt                                                                                                               <  5:57259/149‑1 (MQ=255)
                                     tCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTgg                                                                                                             >  3:340098/1‑149 (MQ=255)
                                     tCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTgg                                                                                                             <  4:304298/149‑1 (MQ=255)
                                       aaCCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGt                                                                                                           <  3:35736/149‑1 (MQ=255)
                                            ccaGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGc                                                                                                                           <  2:131913/128‑1 (MQ=255)
                                            ccaGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGc                                                                                                                           >  1:131913/1‑128 (MQ=255)
                                                aaTGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGtt                                                                                                  <  2:36931/149‑1 (MQ=255)
                                                          gcggcATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTCGACCAGCAgcgca                                                                                                                                       <  6:364248/102‑2 (MQ=255)
                                                          gcggcATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTCGACCAGCAgcgca                                                                                                                                       >  5:364248/1‑101 (MQ=255)
                                                               aTTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCATGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCa                                                                                   >  2:135622/1‑149 (MQ=255)
                                                                     cAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGgcgc                                                                             >  7:93593/1‑149 (MQ=255)
                                                                       ggATGGACGCAATGAAGCCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTGGAGGATTTGTGGGACGAACGGTGTGAACATGTAGTCCACGGcgccg                                                                           >  6:1594/1‑149 (MQ=255)
                                                                        gATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCAc                                                                                  >  3:3034/1‑141 (MQ=255)
                                                                        gATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCAc                                                                                  <  4:3034/141‑1 (MQ=255)
                                                                         aTGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCt                                                                         <  8:93593/149‑1 (MQ=255)
                                                                             aCGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCg                                                                     >  5:244534/1‑149 (MQ=255)
                                                                                 aTTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTggg                                                                                                            <  6:310560/106‑1 (MQ=255)
                                                                                 aTTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTggg                                                                                                            >  5:310560/1‑106 (MQ=255)
                                                                                 aTTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGc                                                                 <  8:71491/149‑1 (MQ=255)
                                                                                  ttGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGcc                                                                <  1:77172/149‑1 (MQ=255)
                                                                                     aaGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCttt                                                             <  1:120030/149‑1 (MQ=255)
                                                                                     aaGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCttt                                                             <  4:187299/149‑1 (MQ=255)
                                                                                     aaGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCttt                                                             >  1:250163/1‑149 (MQ=255)
                                                                                     aaGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCttt                                                             >  1:203319/1‑149 (MQ=255)
                                                                                       ggCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGa                                                           <  1:135622/149‑1 (MQ=255)
                                                                                         cccTGTCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGc                                                         <  2:90395/149‑1 (MQ=255)
                                                                                         cccTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGc                                                         <  2:85333/149‑1 (MQ=255)
                                                                                           cTGGCCGTCTCCAGCTCACGACTCAGCTCCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCAc                                                       >  4:189532/1‑149 (MQ=255)
                                                                                              gCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACgg                                                     <  2:203319/148‑1 (MQ=255)
                                                                                                    tcCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCGTGAGGAGTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCGTTGAGCGCGGCCGCtgc                                              >  8:274991/1‑147 (MQ=255)
                                                                                                      cAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCGACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCtt                                               <  4:340098/146‑1 (MQ=255)
                                                                                                       aGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGa                                           >  7:134848/1‑149 (MQ=255)
                                                                                                        gCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGt                                                                                                   >  5:270153/1‑92 (MQ=255)
                                                                                                        gCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGt                                                                                                   <  6:270153/92‑1 (MQ=255)
                                                                                                            aCGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACtttt                                      >  1:9062/1‑149 (MQ=255)
                                                                                                               aCTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGt                                   <  2:250163/149‑1 (MQ=255)
                                                                                                                    gctgctGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCttt                                                             <  8:347042/118‑1 (MQ=255)
                                                                                                                       gctgcAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGt                            >  3:268663/1‑148 (MQ=255)
                                                                                                                          gcagcaTGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCAGCTGGCGTAGCCTTTGAGCAAGGCCGCTTCGGACTTTTCGTTGACGGTGAg                         >  2:244232/1‑148 (MQ=255)
                                                                                                                             gcaTGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGaa                      >  8:131549/1‑148 (MQ=255)
                                                                                                                             gcaTGCCCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGT‑CACGGCGCCGCATGCGTAGCCATTTAGCACGGCCGCTTCGGACATTTAGTTGGCGGTGAGGaaaa                    >  3:327229/1‑149 (MQ=255)
                                                                                                                                 gcgcAGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGATGGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGa                 >  5:230191/1‑149 (MQ=255)
                                                                                                                                   gcCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGaa                                                                                              <  1:1393/70‑1 (MQ=255)
                                                                                                                                   gcCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGaa                                                                                              >  2:1393/1‑70 (MQ=255)
                                                                                                                                   gcCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCg                                             <  1:127125/119‑1 (MQ=255)
                                                                                                                                   gcCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCg                                             >  2:127125/1‑119 (MQ=255)
                                                                                                                                   gcCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTGGAGGATTTCTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATc               >  5:267572/1‑149 (MQ=255)
                                                                                                                                         ggcgcTGCTCGACCAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGt          >  1:14326/2‑148 (MQ=255)
                                                                                                                                             ctgctgGTCGAGCAGCGCCTGGACCTTAGGCTTGAGGATTTGTGAGTCGAACGGTTTGAACATGTAGTCAACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTc     >  8:55576/1‑149 (MQ=255)
                                                                                                                                                ctgGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCggg  <  7:131549/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AATGGCCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCGGG  >  NC_002947/5650644‑5650936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑